UniProt UniProt http://www.uniprot.org The PAAD/PYRIN-family protein ASC is a dual regulator of a conserved step in nuclear factor kappaB activation pathways. The PAAD/PYRIN-family protein ASC is a dual regulator of a conserved step in nuclear factor kappaB activation pathways. J. Exp. Med. (0022-1007) 2002 imex curation Stehlik C., Fiorentino L., Dorfleutner A., Bruey J.M., Ariza E.M., Sagara J., Reed J.C. For use in the kinase assay, transfected kinases IKKalpha and IKKbeta were immunoprecipitated from HEK293N cells, and washed several times. Origin and range of NFKBIA IkB-alpha as substrate in kinase assays according supplier (Santa Cruz Biotechnology, Inc.). IKKalpha and IKKbeta plasmids have been provided by M. Karin, who originally cloned the respective human sequences (according UniProt). Exact range for used ASC-PAAD construct not provided in the publication. jreed@burnham.org 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. confocal microscopy confocal microscopy immunostaining immunostaining Stehlik C., Fiorentino L., Dorfleutner A., Bruey J.M., Ariza E.M., Sagara J., Reed J.C. For use in the kinase assay, transfected kinases IKKalpha and IKKbeta were immunoprecipitated from HEK293N cells, and washed several times. Origin and range of NFKBIA IkB-alpha as substrate in kinase assays according supplier (Santa Cruz Biotechnology, Inc.). IKKalpha and IKKbeta plasmids have been provided by M. Karin, who originally cloned the respective human sequences (according UniProt). Exact range for used ASC-PAAD construct not provided in the publication. jreed@burnham.org Accepted 2013-JAN-08 by MGT 04-Feb-2013: Contacted by IntAct-Help. J. Exp. Med. (0022-1007) 2002 Only protein-protein interactions imex curation The PAAD/PYRIN-family protein ASC is a dual regulator of a conserved step in nuclear factor kappaB activation pathways. The PAAD/PYRIN-family protein ASC is a dual regulator of a conserved step in nuclear factor kappaB activation pathways. J. Exp. Med. (0022-1007) 2002 imex curation Stehlik C., Fiorentino L., Dorfleutner A., Bruey J.M., Ariza E.M., Sagara J., Reed J.C. For use in the kinase assay, transfected kinases IKKalpha and IKKbeta were immunoprecipitated from HEK293N cells, and washed several times. Origin and range of NFKBIA IkB-alpha as substrate in kinase assays according supplier (Santa Cruz Biotechnology, Inc.). IKKalpha and IKKbeta plasmids have been provided by M. Karin, who originally cloned the respective human sequences (according UniProt). Exact range for used ASC-PAAD construct not provided in the publication. jreed@burnham.org 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Stehlik C., Fiorentino L., Dorfleutner A., Bruey J.M., Ariza E.M., Sagara J., Reed J.C. For use in the kinase assay, transfected kinases IKKalpha and IKKbeta were immunoprecipitated from HEK293N cells, and washed several times. Origin and range of NFKBIA IkB-alpha as substrate in kinase assays according supplier (Santa Cruz Biotechnology, Inc.). IKKalpha and IKKbeta plasmids have been provided by M. Karin, who originally cloned the respective human sequences (according UniProt). Exact range for used ASC-PAAD construct not provided in the publication. jreed@burnham.org Accepted 2013-JAN-08 by MGT 04-Feb-2013: Contacted by IntAct-Help. J. Exp. Med. (0022-1007) 2002 Only protein-protein interactions imex curation The PAAD/PYRIN-family protein ASC is a dual regulator of a conserved step in nuclear factor kappaB activation pathways. The PAAD/PYRIN-family protein ASC is a dual regulator of a conserved step in nuclear factor kappaB activation pathways. J. Exp. Med. (0022-1007) 2002 imex curation Stehlik C., Fiorentino L., Dorfleutner A., Bruey J.M., Ariza E.M., Sagara J., Reed J.C. For use in the kinase assay, transfected kinases IKKalpha and IKKbeta were immunoprecipitated from HEK293N cells, and washed several times. Origin and range of NFKBIA IkB-alpha as substrate in kinase assays according supplier (Santa Cruz Biotechnology, Inc.). IKKalpha and IKKbeta plasmids have been provided by M. Karin, who originally cloned the respective human sequences (according UniProt). Exact range for used ASC-PAAD construct not provided in the publication. jreed@burnham.org 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Stehlik C., Fiorentino L., Dorfleutner A., Bruey J.M., Ariza E.M., Sagara J., Reed J.C. For use in the kinase assay, transfected kinases IKKalpha and IKKbeta were immunoprecipitated from HEK293N cells, and washed several times. Origin and range of NFKBIA IkB-alpha as substrate in kinase assays according supplier (Santa Cruz Biotechnology, Inc.). IKKalpha and IKKbeta plasmids have been provided by M. Karin, who originally cloned the respective human sequences (according UniProt). Exact range for used ASC-PAAD construct not provided in the publication. jreed@burnham.org Accepted 2013-JAN-08 by MGT 04-Feb-2013: Contacted by IntAct-Help. J. Exp. Med. (0022-1007) 2002 Only protein-protein interactions imex curation The PAAD/PYRIN-family protein ASC is a dual regulator of a conserved step in nuclear factor kappaB activation pathways. The PAAD/PYRIN-family protein ASC is a dual regulator of a conserved step in nuclear factor kappaB activation pathways. J. Exp. Med. (0022-1007) 2002 imex curation Stehlik C., Fiorentino L., Dorfleutner A., Bruey J.M., Ariza E.M., Sagara J., Reed J.C. For use in the kinase assay, transfected kinases IKKalpha and IKKbeta were immunoprecipitated from HEK293N cells, and washed several times. Origin and range of NFKBIA IkB-alpha as substrate in kinase assays according supplier (Santa Cruz Biotechnology, Inc.). IKKalpha and IKKbeta plasmids have been provided by M. Karin, who originally cloned the respective human sequences (according UniProt). Exact range for used ASC-PAAD construct not provided in the publication. jreed@burnham.org 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Stehlik C., Fiorentino L., Dorfleutner A., Bruey J.M., Ariza E.M., Sagara J., Reed J.C. For use in the kinase assay, transfected kinases IKKalpha and IKKbeta were immunoprecipitated from HEK293N cells, and washed several times. Origin and range of NFKBIA IkB-alpha as substrate in kinase assays according supplier (Santa Cruz Biotechnology, Inc.). IKKalpha and IKKbeta plasmids have been provided by M. Karin, who originally cloned the respective human sequences (according UniProt). Exact range for used ASC-PAAD construct not provided in the publication. jreed@burnham.org Accepted 2013-JAN-08 by MGT 04-Feb-2013: Contacted by IntAct-Help. J. Exp. Med. (0022-1007) 2002 Only protein-protein interactions imex curation The PAAD/PYRIN-family protein ASC is a dual regulator of a conserved step in nuclear factor kappaB activation pathways. The PAAD/PYRIN-family protein ASC is a dual regulator of a conserved step in nuclear factor kappaB activation pathways. J. Exp. Med. (0022-1007) 2002 imex curation Stehlik C., Fiorentino L., Dorfleutner A., Bruey J.M., Ariza E.M., Sagara J., Reed J.C. For use in the kinase assay, transfected kinases IKKalpha and IKKbeta were immunoprecipitated from HEK293N cells, and washed several times. Origin and range of NFKBIA IkB-alpha as substrate in kinase assays according supplier (Santa Cruz Biotechnology, Inc.). IKKalpha and IKKbeta plasmids have been provided by M. Karin, who originally cloned the respective human sequences (according UniProt). Exact range for used ASC-PAAD construct not provided in the publication. jreed@burnham.org 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions human-thp-1 Homo sapiens acute monocytic leukemia cells thp-1 Human acute monocytic leukemia cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Stehlik C., Fiorentino L., Dorfleutner A., Bruey J.M., Ariza E.M., Sagara J., Reed J.C. For use in the kinase assay, transfected kinases IKKalpha and IKKbeta were immunoprecipitated from HEK293N cells, and washed several times. Origin and range of NFKBIA IkB-alpha as substrate in kinase assays according supplier (Santa Cruz Biotechnology, Inc.). IKKalpha and IKKbeta plasmids have been provided by M. Karin, who originally cloned the respective human sequences (according UniProt). Exact range for used ASC-PAAD construct not provided in the publication. jreed@burnham.org Accepted 2013-JAN-08 by MGT 04-Feb-2013: Contacted by IntAct-Help. J. Exp. Med. (0022-1007) 2002 Only protein-protein interactions imex curation ikba_human NF-kappa-B inhibitor alpha NFKBIA IKBA MAD3 NFKBI I-kappa-B-alpha Major histocompatibility complex enhancer-binding protein MAD3 protein protein human Homo sapiens Human MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL DIP protein P25963 original sequence version: 160 DIP protein P25963 original sequence version: 157 DIP protein P25963 original sequence version: 145 DIP protein P25963 original sequence version: 170 DIP protein P25963 original sequence version: 122 DIP protein P25963 original sequence version: 128 DIP protein P25963 original sequence version: 78 DIP protein P25963 original sequence version: 121 DIP protein P25963 original sequence version: 124 088B313226786395 nemo_human NF-kappa-B essential modulator IKBKG FIP3 NEMO NF-kappa-B essential modifier Inhibitor of nuclear factor kappa-B kinase subunit gamma IkB kinase-associated protein 1 FIP-3 protein protein human Homo sapiens Human MNRHLWKSQLCEMVQPSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPETLQRCLEENQELRDAIRQSNQILRERCEELLHFQASQREEKEFLMCKFQEARKLVERLGLEKLDLKRQKEQALREVEHLKRCQQQMAEDKASVKAQVTSLLGELQESQSRLEAATKECQALEGRARAASEQARQLESEREALQQQHSVQVDQLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVVGSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLKEEAEQHKIVMETVPVLKAQADIYKADFQAERQAREKLAEKKELLQEQLEQLQREYSKLKASCQESARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE DIP protein Q9Y6K9 original sequence version: 85 DIP protein Q9Y6K9 original sequence version: 118 DIP protein Q9Y6K9 original sequence version: 123 DIP protein Q9Y6K9 original sequence version: 141 DIP protein Q9Y6K9 original sequence version: 125 DIP protein Q9Y6K9 original sequence version: 156 DIP protein Q9Y6K9 original sequence version: 83 DIP protein Q9Y6K9 original sequence version: 132 DIP protein Q9Y6K9 original sequence version: 124 DIP protein Q9Y6K9 original sequence version: 90 322D1037881447FF ikka_human Inhibitor of nuclear factor kappa-B kinase subunit alpha CHUK IKKA TCF16 I-kappa-B kinase 1 Conserved helix-loop-helix ubiquitous kinase Nuclear factor NF-kappa-B inhibitor kinase alpha Transcription factor 16 protein protein human Homo sapiens Human MERPPGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIKSCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPEELNILIHDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKIIHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMSGEVRFSSHLPQPNSLCSLVVEPMENWLQLMLNWDPQQRGGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSAKIISFLLPPDESLHSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLISASQQLKAKLEFFHKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYAEVGVIGYLEDQIMSLHAEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPSDHSYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGAVTPQTSAWLPPTSAEHDHSLSCVVTPQDGETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDWSWLTE DIP protein O15111 original sequence version: 91 DIP protein O15111 original sequence version: 123 DIP protein O15111 original sequence version: 133 DIP protein O15111 original sequence version: 118 DIP protein O15111 original sequence version: 125 DIP protein O15111 original sequence version: 96 4EA55C6FFC66FA16 ikkb_human Inhibitor of nuclear factor kappa-B kinase subunit beta IKBKB IKKB I-kappa-B kinase 2 Nuclear factor NF-kappa-B inhibitor kinase beta Serine/threonine protein kinase IKBKB protein protein human Homo sapiens Human MSWSPSLTTQTCGAWEMKERLGTGGFGNVIRWHNQETGEQIAIKQCRQELSPRNRERWCLEIQIMRRLTHPNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRKYLNQFENCCGLREGAILTLLSDIASALRYLHENRIIHRDLKPENIVLQQGEQRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWHSKVRQKSEVDIVVSEDLNGTVKFSSSLPYPNNLNSVLAERLEKWLQLMLMWHPRQRGTDPTYGPNGCFKALDDILNLKLVHILNMVTGTIHTYPVTEDESLQSLKARIQQDTGIPEEDQELLQEAGLALIPDKPATQCISDGKLNEGHTLDMDLVFLFDNSKITYETQISPRPQPESVSCILQEPKRNLAFFQLRKVWGQVWHSIQTLKEDCNRLQQGQRAAMMNLLRNNSCLSKMKNSMASMSQQLKAKLDFFKTSIQIDLEKYSEQTEFGITSDKLLLAWREMEQAVELCGRENEVKLLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKPRDQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVEEVVSLMNEDEKTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNASRLSQPGQLMSQPSTASNSLPEPAKKSEELVAEAHNLCTLLENAIQDTVREQDQSFTALDWSWLQTEEEEHSCLEQAS DIP protein O14920 original sequence version: 89 DIP protein O14920 original sequence version: 100 DIP protein O14920 original sequence version: 121 DIP protein O14920 original sequence version: 126 DIP protein O14920 original sequence version: 136 DIP protein O14920 original sequence version: 128 DIP protein O14920-1 original sequence version: 151 DIP protein O14920 original sequence version: 142 DIP protein O14920 original sequence version: 159 DIP protein O14920 original sequence version: 93 DIP protein O14920 original sequence version: 124 DIP protein O14920-1 original sequence version: 178 DIP protein O14920 original sequence version: 85 F9CADF671AE9E14E asc_human Apoptosis-associated speck-like protein containing a CARD PYD and CARD domain-containing protein Target of methylation-induced silencing 1 Caspase recruitment domain-containing protein 5 ASC CARD5 TMS1 PYCARD protein protein human Homo sapiens Human MGRARDAILDALENLTAEELKKFKLKLLSVPLREGYGRIPRGALLSMDALDLTDKLVSFYLETYGAELTANVLRDMGLQEMAGQLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS DIP protein Q9ULZ3 original sequence version: 116 455987286586F46A chuk-nfkbia-11 4 6 enzyme target enzyme target substrate substrate neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 enzyme enzyme neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O15111:p.Lys44Met mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain K M Wed May 02 14:40:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys44met' Tue Jun 30 18:05:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys44Met' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. phosphorylation phosphorylation reaction phosphorylation 3B PYCARD/ASC (UniProt Ac Q9ULZ3) DAPIN domain or full length PYCARD/ASC TNF-alpha chuk-chuk-4 4 8 putative self putative self putative self putative self nucl transfection nucleic acid transfection nucl transfection O15111:p.Lys44Met mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain K M Wed May 02 15:13:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys44met' Tue Jun 30 17:10:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys44Met' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. phosphorylation phosphorylation reaction phosphorylation true 3B PYCARD/ASC (UniProt Ac Q9ULZ3) DAPIN domain or full length PYCARD/ASC TNF-alpha chuk-ikbkb-1 4 6 enzyme target enzyme target substrate substrate neutral component neutral component purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 enzyme enzyme neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O14920:p.Lys44Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:50:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys44ala' Tue Jun 30 18:09:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys44Ala' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. phosphorylation phosphorylation reaction phosphorylation 3C PYCARD/ASC (UniProt Ac Q9ULZ3) DAPIN domain or full length PYCARD/ASC. TNF-alpha chuk-nfkbia-14 4 6 enzyme target enzyme target substrate substrate neutral component neutral component purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 enzyme enzyme neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. phosphorylation phosphorylation reaction phosphorylation 3D PYCARD/ASC (UniProt Ac Q9ULZ3) DAPIN domain TRAF2 and TRAF6. chuk-nfkbia-15 4 6 enzyme target enzyme target substrate substrate neutral component neutral component purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 enzyme enzyme neutral component neutral component human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. phosphorylation phosphorylation reaction phosphorylation 3E PYCARD/ASC (UniProt Ac Q9ULZ3) DAPIN domain TNFalpha chuk-ikbkb-2 5 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait association association 4A pycard-chuk 5 10 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey association association 4A chuk-ikbkb-4 2 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range dapin_domain sufficient to bind sufficient binding region sufficient to bind range range range range association association 4B pycard-chuk-2 3 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range dapin_domain sufficient to bind sufficient binding region sufficient to bind range range range range 9 unspecified role unspecified role prey prey association association 4B ikbkb-chuk-5 2 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection dapin_domain sufficient to bind sufficient binding region sufficient to bind range range range range n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association 4B chuk-pycard 1 10 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range dapin_domain sufficient to bind sufficient binding region sufficient to bind range range range range 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 4C chuk-ikbkb-5 1 10 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection dapin_domain sufficient to bind sufficient binding region sufficient to bind range range range range n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role neutral component neutral component colocalization colocalization 4D chuk-nfkbia-16 4 8 enzyme enzyme neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O15111:p.Lys44Met mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain K M Wed May 02 20:23:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys44met' Tue Jun 30 20:21:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys44Met' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 6 enzyme target enzyme target substrate substrate neutral component neutral component purified purified human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. phosphorylation phosphorylation reaction phosphorylation 3B PYCARD/ASC (UniProt Ac Q9ULZ3) DAPIN domain or full length PYCARD/ASC TNF-alpha chuk-1 4 8 putative self putative self putative self putative self human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. phosphorylation phosphorylation reaction phosphorylation true 3E PYCARD/ASC (UniProt Ac Q9ULZ3) DAPIN domain TNFalpha