IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structure and catalytic mechanism of the E. coli chemotaxis phosphatase CheZ. Structure and catalytic mechanism of the E. coli chemotaxis phosphatase CheZ. Nat. Struct. Biol. (1072-8368) 2002 imex curation Zhao R., Collins E.J., Bourret R.B., Silversmith R.E. silversr@med.unc.edu 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Zhao R., Collins E.J., Bourret R.B., Silversmith R.E. silversr@med.unc.edu Accepted 2012-DEC-18 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. Nat. Struct. Biol. (1072-8368) 2002 Only protein-protein interactions imex curation Structure and catalytic mechanism of the E. coli chemotaxis phosphatase CheZ. Structure and catalytic mechanism of the E. coli chemotaxis phosphatase CheZ. Nat. Struct. Biol. (1072-8368) 2002 imex curation Zhao R., Collins E.J., Bourret R.B., Silversmith R.E. silversr@med.unc.edu 04-Feb-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zhao R., Collins E.J., Bourret R.B., Silversmith R.E. silversr@med.unc.edu Accepted 2012-DEC-18 by ORCHARD 04-Feb-2013: Contacted by IntAct-Help. Nat. Struct. Biol. (1072-8368) 2002 Only protein-protein interactions imex curation chez_ecoli Protein phosphatase CheZ cheZ b1881 JW1870 Chemotaxis protein CheZ protein protein ecoli Escherichia coli (strain K12) MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEAAEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPIDLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIERQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF DIP protein P0A9H9 original sequence version: 75 42308F5AC0685D27 chey_ecoli Chemotaxis protein CheY cheY b1882 JW1871 protein protein ecoli Escherichia coli (strain K12) MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALNKLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAGASGYVVKPFTAATLEEKLNKIFEKLGM DIP protein P0AE67 original sequence version: 95 DIP protein P0AE67 original sequence version: 56 DIP protein P0AE67 original sequence version: 96 E4B60B8A73DA14DC chey-chez-2 2 4 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role neutral component neutral component P0A9H9:p.Glu134Lys mutation mutation certain certain sequence position certain certain certain sequence position certain E K Wed May 02 14:59:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu134lys' Tue Jun 30 16:15:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu134Lys' ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 1 2.9 A R value: 0.279 R free: 0.298 chey-chez-4 1 3 competitor competitor competitor neutral component neutral component P0A9H9:p.Gln147Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 20:10:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln147ala' Tue Jun 30 16:59:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln147Ala' ecoli Escherichia coli (strain K12) 4 competitor competitor competitor neutral component neutral component P0AE67:p.Asn59Arg mutation mutation certain certain sequence position certain certain certain sequence position certain N R Wed May 02 19:30:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn59arg' Tue Jun 30 16:06:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn59Arg' ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role neutral component neutral component fluorescein fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 2C