IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ C. elegans CED-12 acts in the conserved crkII/DOCK180/Rac pathway to control cell migration and cell corpse engulfment. C. elegans CED-12 acts in the conserved crkII/DOCK180/Rac pathway to control cell migration and cell corpse engulfment. Dev. Cell (1534-5807) 2001 imex curation Wu YC., Tsai MC., Cheng LC., Chou CJ., Weng NY. http://dx.doi.org/10.1016/S1534-5807(01)00056-9 yichun@ccms.ntu.edu.tw 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 3 hybrid three hybrid 3-hybrid tri hybrid tri-hybrid assay 3 hybrid predetermined predetermined participant predetermined Wu YC., Tsai MC., Cheng LC., Chou CJ., Weng NY. http://dx.doi.org/10.1016/S1534-5807(01)00056-9 yichun@ccms.ntu.edu.tw Accepted 2012-NOV-16 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. Dev. Cell (1534-5807) 2001 Only protein-protein interactions imex curation C. elegans CED-12 acts in the conserved crkII/DOCK180/Rac pathway to control cell migration and cell corpse engulfment. C. elegans CED-12 acts in the conserved crkII/DOCK180/Rac pathway to control cell migration and cell corpse engulfment. Dev. Cell (1534-5807) 2001 imex curation Wu YC., Tsai MC., Cheng LC., Chou CJ., Weng NY. http://dx.doi.org/10.1016/S1534-5807(01)00056-9 yichun@ccms.ntu.edu.tw 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Wu YC., Tsai MC., Cheng LC., Chou CJ., Weng NY. http://dx.doi.org/10.1016/S1534-5807(01)00056-9 yichun@ccms.ntu.edu.tw Accepted 2012-NOV-16 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. Dev. Cell (1534-5807) 2001 Only protein-protein interactions imex curation C. elegans CED-12 acts in the conserved crkII/DOCK180/Rac pathway to control cell migration and cell corpse engulfment. C. elegans CED-12 acts in the conserved crkII/DOCK180/Rac pathway to control cell migration and cell corpse engulfment. Dev. Cell (1534-5807) 2001 imex curation Wu YC., Tsai MC., Cheng LC., Chou CJ., Weng NY. http://dx.doi.org/10.1016/S1534-5807(01)00056-9 yichun@ccms.ntu.edu.tw 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Wu YC., Tsai MC., Cheng LC., Chou CJ., Weng NY. http://dx.doi.org/10.1016/S1534-5807(01)00056-9 Interaction between DOCK180 and KIAA0281 not captured as origin of DOCK180 not given. yichun@ccms.ntu.edu.tw Accepted 2012-NOV-16 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. Dev. Cell (1534-5807) 2001 Only protein-protein interactions imex curation ced12_caeel Cell death abnormality protein 12 ced-12 Y106G6E.5 protein protein caeel Caenorhabditis elegans MAFHMPLKELQPVDTSLPEHVVKGAVVIDKEFTIWNHRAVIPSNALHTVFITINLLEQKLTDVVKMAARSMNLSEDDSYGLMADKPKRFITDDNLNSLGSGFILTLCASPDHYVKRITEILTEGNNISQMENAVKTLDEFSLDPALIEAFYRCSSLELLFSLIRDDRVCMSSTLLSTCLRALSSMLELAVGDFTWKSVPNDVVVSMASLVTGKAKREEANTLLAALQMLEQLVIGDDTTRDWILEEVPIETLIRHVEKSDERIALCALSLMNSMIRRCPDDEKRFELIKSLEVVPFRNAVHSSLLRGGGGVRNLNAIEQLVEVQRSLISAYETSPPTDAEVQKILDIESSEDVSEEIREMWKSQIGEHRCGRLAAISMVQFAEKSPQDLRMLISENTMRIEGGKWQLIPMWMRCCDIAAELFRVIPGRDELDRLIVVLFSTETPFPAVFACIVHLFHRTWREMQAKGGEMEKVACVVLEQLRHVLKRREIQDVEELSADLETFSYRAMQEIWREEQLGKENIQLHSEAVIQLKSKLRPKMEELVRINHLNYLKLGAVFRKPQKSKSLAKLAFWHWKLDASEKMLTITGCDGENYVEGVQRDDIRQVWIKDIADVTNNDEIDRKASSSRFTSSPSTQMLRGIRVQLKTTNDMKEGEVLMALTSDETQSVIWLEGLAELIGSKAVKSETDAMVERMLKMELRVRLLNVKLTNPEEKPEIPPIPDDIKSFISKF 5243F167AE0C868B ced2_caeel Cell death abnormality protein 2 ced-2 Cell-corpse engulfment protein CED-2 Y41D4B.13 protein protein caeel Caenorhabditis elegans MTTNGFDPFEWRSFYFPGMSREEAHKLLGEPQVSIGTFLMRDSSRPGEYSLTVREADEGNAVCHYLIERGEPKEDGTAAAGVKIANQSFPDIPALLNHFKMRVLTEASLLAAYKKPIIEVVVGTFKFTGERETDLPFEQGERLEILSKTNQDWWEARNALGTTGLVPANYVQIQMEFHNDRTSKGASQSSIGSSGGGAERFSSASTSSDNIELQPRLPAKAKVTFDRVPNAYDPTQLRVKKGQTVLVTQKMSNGMYKAELDGQIGSVPHTYLRFTAVSE DIP protein Q9NHC3 original sequence version: 72 5CE7DA478948970B g5een3_caeel C02F4.1 ced-5 CELE_C02F4.1 protein protein caeel Caenorhabditis elegans MATTGFYYAIVQYTFEDPLPSCDAPRLELFIGDRICVYGQHGEWGYGRKFDEKNGKCGIFPLAYVQIVQKSMFVSTSDGYLVVDEISRVINEWWTKIKELMVETTRIGSFEDLMDSFNELLIIKTKIESGGIPIEELSKLRLRVSKLVDRGNTILGQDVVIRNEEGVPLDVESLSLLRTYEAHISSKGRVGSLMREKPENVTINDSFSLLLSIKSVELHCKYSCEISISLYDLDKKMFTTDSYTFLWNSGSGKHTDLNLKALFTDFAKEDIQKKYLMVTRVVHVSPIESSNATMRKHGHHEATIPKTFYCRQSYASDIMEMSSIFLAGVGHEAKERVIFLNREPELPLSLKAYHATNRIPKNLSNDMETKLLISTQLVPGNVSQIKARHPHLFSRCPAAILRRADRTAVSIEDSRNEMYITLMQAELSGKSSDRNIEARLHVVESNGHVMENVFETISVTGSQLSTVYKSIVVYHTDKPMWTEPIKIALPSCASHDVYLRILFYSKKAYDKPKPEKGPFAIAHVQLIRSSALLCDGEHDLAVYKIDNPGSHFDESNVHYMNLPDTRRTLKESIGSAKPHSQGFSLSDKSFVMISTHSCSSMLTQNEHLLNVLRWRVNCVNLTSSLVALAQPIGDTEHEMIRFFSHLLDALFEIWHDRETSEKVVFDVIVAVLRICEEPRHYPQAAKIFEVYLKRFSFTSAALKILKCLNHYVISDSEDSNEKARNAFKVMGPLFKLVVVSKKCGIKFEEFDDFEKTYKQYLREFMKSLVALMSEKKAKMTVQNTALKSIPTIIDLLNESDSVSPENLCGFIVDLMNNFGSNIVTRERLNFIAQIVETRFFSLSICREQLLIPCLQIALDIIQIDNMATEKGEFADRAAECASIIAAILERLFADAKSGEGNAERELTSFILMVYRPLVQAMIRVIHDDKHTDDDARGHFFSVILALLDKMSAQMFSEYVEERSSDIDKRDFLMEMVQMIRDLLNRNAFPSTWKDMIMLQNKVIHKSLRFVMSAVQTFFSNDKFCVEMWREYMVTVVSFVTQEGLNSKHEWMKNEDEDMRIQLRKAAAKDLRSMWFRLTPSQKLNYIPSMIGSFLKVSLVDDDETREATIPIFFDMMQTEYNTSASRSFTEFASELVSQLDTNVDQHSATKGFKEHFRQLSITLCQSDKELMANGGEELIERIDRLLTALIEYHEVASKSLVECVDSLMSRTVQLMRYYNQYSHKELYVKYIYKLYDLHTSYGNKIEAAKTLLRHATMLTFDDDALPQWLIARSLNRHRELHRQLKEDLMEEAGNLFSKGEDWEDALIVYNQLVPVYQNIIMDYDKLAGLLQKIAQLYTSISRTERAYFYYYLVAFYGQGFPAYLNGHKFVFRSEKLEMHGEFMQRIMKMYDNPEKIMKTDPCPHLVDSPGRYIQVFNIDPIGTGCSFENNPEVKPVIKKYFRYYNIQTFEYSKVEERKDTKWTSIDPSSEFMRNWLVRRRIKTADSLPTDLRFTEIVELSDPIYVTPLQNAVEQMRKKNKELNETAASAESNPNFDLKLLSRDILGVVSAAVMGGVKNYEVFFTEACRNICECGEQSVIMELSSLIIEQVEILEYCCYVHASRCQGEARAINTMLADSFDSHRRYVEENFGKTRSRLPTHANIRLSSFDSDSINDGMMTMKSGKAAVLGNAVANLIYSNKRSSGPGTPVNLSSRSAPSFDNISSKLRSSLAISKLAPPPRPPNPISPINRGPSSNYEGIRMRAHSHQASTVSLNHMSVAPPLPPRPDRPDMDPNRTLKRKN 96058311215D67E2 ced_12-ced_5-1 2 ced-12 6 unspecified role unspecified role prey prey purified purified region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ced-5_1415-1781 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro ced-12N 4 unspecified role unspecified role bait bait ced-12_397-731 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag autoradiography autoradiography undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 3 A ced_12-ced_5-2 2 ced-12C 4 unspecified role unspecified role bait bait gst gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ced-12n sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ced-5 6 unspecified role unspecified role prey prey purified purified 35s 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 autoradiography autoradiography undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ced-5_1415-1781 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro physical association physical association and less strongly with the N-terminal region of CED-12-GST fusion Figure 3 A ced_5-ced_12 3 6 unspecified role unspecified role bait bait ced-5_1415-1689 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey ced-12_397-731 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 23 25 physical association physical association Figure 3. ced-2_ced-5_ced-12 1 5 unspecified role unspecified role prey prey electroporation electroporation ced-12C 4 unspecified role unspecified role bait bait ced-12_397-731 sufficient to bind sufficient binding region sufficient to bind protein staining protein staining certain certain sequence position certain certain certain sequence position certain ced-5C 6 unspecified role unspecified role ancillary ancillary electroporation electroporation ced-5_1415-1781 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 3.