IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Caenorhabditis elegans hif-1 gene encodes a bHLH-PAS protein that is required for adaptation to hypoxia. The Caenorhabditis elegans hif-1 gene encodes a bHLH-PAS protein that is required for adaptation to hypoxia. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2001 imex curation Jiang H., Guo R., Powell-Coffman J.A. HIF-1 Binds AHA-1. The mammalian hypoxia-inducible factors consist of two protein subunits: HIF-1 alpha (or the related HIF-2 alpha or HIF-3 alpha) and ARNT (or ARNT2 or ARNT3). To determine whether C. elegans HIF-1 forms a similar complex, we assayed its ability to bind AHA-1, the ortholog of mammalian ARNT. We expressed both gene products in rabbit reticulocyte lysates and immunoprecipitated the proteins with an antibody that specifically recognizes AHA-1. When HIF-1 is coincubated with AHA-1, both proteins are immunoprecipitated (Fig. 2.2, lane 5). Thus, HIF-1 binds AHA-1 in vitro. japc@iastate.edu 19-Jun-2012: Contacted by JYOTI. Only protein-protein interactions rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Jiang H., Guo R., Powell-Coffman J.A. HIF-1 Binds AHA-1. The mammalian hypoxia-inducible factors consist of two protein subunits: HIF-1 alpha (or the related HIF-2 alpha or HIF-3 alpha) and ARNT (or ARNT2 or ARNT3). To determine whether C. elegans HIF-1 forms a similar complex, we assayed its ability to bind AHA-1, the ortholog of mammalian ARNT. We expressed both gene products in rabbit reticulocyte lysates and immunoprecipitated the proteins with an antibody that specifically recognizes AHA-1. When HIF-1 is coincubated with AHA-1, both proteins are immunoprecipitated (Fig. 2.2, lane 5). Thus, HIF-1 binds AHA-1 in vitro. japc@iastate.edu Accepted 2012-FEB-28 by ORCHARD Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2001 Only protein-protein interactions imex curation hif1_caeel Hypoxia-inducible factor 1 hif-1 Hypoxia-induced factor 1 F38A6.3 protein protein caeel Caenorhabditis elegans MEDNRKRNMERRRETSRHAARDRRSKESDIFDDLKMCVPIVEEGTVTHLDRIALLRVAATICRLRKTAGNVLENNLDNEITNEVWTEDTIAECLDGFVMIVDSDSSILYVTESVAMYLGLTQTDLTGRALRDFLHPSDYDEFDKQSKMLHKPRGEDTDTTGINMVLRMKTVISPRGRCLNLKSALYKSVSFLVHSKVSTGGHVSFMQGITIPAGQGTTNANASAMTKYTESPMGAFTTRHTCDMRITFVSDKFNYILKSELKTLMGTSFYELVHPADMMIVSKSMKELFAKGHIRTPYYRLIAANDTLAWIQTEATTITHTTKGQKGQYVICVHYVLGIQGAEESLVVCTDSMPAGMQVDIKKEVDDTRDYIGRQPEIVECVDFTPLIEPEDPFDTVIEPVVGGEEPVKQADMGARKNSYDDVLQWLFRDQPSSPPPARYRSADRFRTTEPSNFGSALASPDFMDSSSRTSRPKTSYGRRAQSQGSRTTGSSSTSASATLPHSANYSPLAEGISQCGLNSPPSIKSGQVVYGDARSMGRSCDPSDSSRRFSALSPSDTLNVSSTRGINPVIGSNDVFSTMPFADSIAIAERIDSSPTLTSGEPILCDDLQWEEPDLSCLAPFVDTYDMMQMDEGLPPELQALYDLPDFTPAVPQAPAARPVHIDRSPPAKRMHQSGPSDLDFMYTQHYQPFQQDETYWQGQQQQNEQQPSSYSPFPMLS E6F5762100E179FE aha1_caeel Aryl hydrocarbon receptor nuclear translocator homolog aha-1 AHR-associated protein C25A1.11 protein protein caeel Caenorhabditis elegans MAQDIFMDPWQSATSFAMEDEDMGMPSGKYARMEDEMGENKERFARENHSEIERRRRNKMTHYINELAEMVPQCASLGRKPDKLTILRMAVSHMKGIRGHTAQDETSYKPSFLTDQELKHLILEAANGFLFVVCCQTGKVLYVADSITPVLNLKQEDWLQRNLNELIHPDDQDKIRDQLCGSEVSVNKVLDLKSGSVKREGASTRVHMSCRRGFICRMRVGALEPLHRLRNRRPLFQHAGQNYVVMHCTGYIKNAPPQGINAPASSCLVAIARLQVASMPVCADPTSTNQFSVRVSEDGKMTFIDARVSDLIGLSSDQLIGRYWWNLAHPADEKTLQDSFVALLSDQPMRINIRVRTSTDYIPCTVSAYKFMNPYSEQFEYVVATHQIAPQEDINNWVTAPTVPQPQASEFGELGGAPSAVDYGQSSSGGWRPEAQGAPQAQWQWDPMNGYNQ B00FFE005C9B0BCB aha_1-hif_1-1 1 2 unspecified role unspecified role prey prey hif-1-35s 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 autoradiography autoradiography undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 3 unspecified role unspecified role bait bait aha-1-35s 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 autoradiography autoradiography undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association