mbinfo MBInfo http://www.mechanobio.info/ Activation of Arp2/3 complex-mediated actin polymerization by cortactin. Activation of Arp2/3 complex-mediated actin polymerization by cortactin. Nat. Cell Biol. (1465-7392) 2001 imex curation Uruno T., Liu J., Zhang P., Fan Yx , Egile C., Li R., Mueller S.C., Zhan X. Interaction detection method: GST pull down. The affinity between the proteins was further quantitated by immunoblotting Arp3 from the supernatants and thus calculating the percentage of Arp3 depletion from supernatant due to binding to cortactin/N-WASP. The source of Arp3 and cortactin specified by the author. The source of N-WASP traced from references. zhanx@usa.redcross.org 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells confocal microscopy confocal microscopy immunostaining immunostaining Uruno T., Liu J., Zhang P., Fan Yx , Egile C., Li R., Mueller S.C., Zhan X. Interaction detection method: GST pull down. The affinity between the proteins was further quantitated by immunoblotting Arp3 from the supernatants and thus calculating the percentage of Arp3 depletion from supernatant due to binding to cortactin/N-WASP. The source of Arp3 and cortactin specified by the author. The source of N-WASP traced from references. zhanx@usa.redcross.org Accepted 2012-AUG-14 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Nat. Cell Biol. (1465-7392) 2001 Only protein-protein interactions imex curation Activation of Arp2/3 complex-mediated actin polymerization by cortactin. Activation of Arp2/3 complex-mediated actin polymerization by cortactin. Nat. Cell Biol. (1465-7392) 2001 imex curation Uruno T., Liu J., Zhang P., Fan Yx , Egile C., Li R., Mueller S.C., Zhan X. Interaction detection method: GST pull down. The affinity between the proteins was further quantitated by immunoblotting Arp3 from the supernatants and thus calculating the percentage of Arp3 depletion from supernatant due to binding to cortactin/N-WASP. The source of Arp3 and cortactin specified by the author. The source of N-WASP traced from references. zhanx@usa.redcross.org 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Uruno T., Liu J., Zhang P., Fan Yx , Egile C., Li R., Mueller S.C., Zhan X. Interaction detection method: GST pull down. The affinity between the proteins was further quantitated by immunoblotting Arp3 from the supernatants and thus calculating the percentage of Arp3 depletion from supernatant due to binding to cortactin/N-WASP. The source of Arp3 and cortactin specified by the author. The source of N-WASP traced from references. zhanx@usa.redcross.org Accepted 2012-AUG-14 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Nat. Cell Biol. (1465-7392) 2001 Only protein-protein interactions imex curation Activation of Arp2/3 complex-mediated actin polymerization by cortactin. Activation of Arp2/3 complex-mediated actin polymerization by cortactin. Nat. Cell Biol. (1465-7392) 2001 imex curation Uruno T., Liu J., Zhang P., Fan Yx , Egile C., Li R., Mueller S.C., Zhan X. Interaction detection method: GST pull down. The affinity between the proteins was further quantitated by immunoblotting Arp3 from the supernatants and thus calculating the percentage of Arp3 depletion from supernatant due to binding to cortactin/N-WASP. The source of Arp3 and cortactin specified by the author. The source of N-WASP traced from references. zhanx@usa.redcross.org 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma confocal microscopy confocal microscopy immunostaining immunostaining Uruno T., Liu J., Zhang P., Fan Yx , Egile C., Li R., Mueller S.C., Zhan X. Interaction detection method: GST pull down. The affinity between the proteins was further quantitated by immunoblotting Arp3 from the supernatants and thus calculating the percentage of Arp3 depletion from supernatant due to binding to cortactin/N-WASP. The source of Arp3 and cortactin specified by the author. The source of N-WASP traced from references. zhanx@usa.redcross.org Accepted 2012-AUG-14 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Nat. Cell Biol. (1465-7392) 2001 Only protein-protein interactions imex curation arp3_mouse Actin-related protein 3 Actin-like protein 3 Actr3 Arp3 protein protein mouse Mus musculus Mouse MAGRLPACVVDCGTGYTKLGYAGNTEPQFIIPSCIAIKESAKVGDQAQRRVMKGVDDLDFFIGDEAIEKPTYATKWPIRHGIVEDWDLMERFMEQVIFKYLRAEPEDHYFLLTEPPLNTPENREYTAEIMFESFNVPGLYIAVQAVLALAASWTSRQVGERTLTGTVIDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRDITYFIQQLLRDREVGIPPEQSLETAKAVKERYSYVCPDLVKEFNKYDTDGSKWIKQYTGVNAISKKEFSIDVGYERFLGPEIFFHPEFANPDFTQPISEVVDEVIQNCPIDVRRPLYKNIVLSGGSTMFRDFGRRLQRDLKRTVDARLKLSEELSGGRLKPKPIDVQVITHHMQRYAVWFGGSMLASTPEFYQVCHTKKDYEEIGPSICRHNPVFGVMS 806FED7A08ABA455 arp3_human Actin-related protein 3 ACTR3 ARP3 Actin-like protein 3 protein protein human Homo sapiens Human MAGRLPACVVDCGTGYTKLGYAGNTEPQFIIPSCIAIKESAKVGDQAQRRVMKGVDDLDFFIGDEAIEKPTYATKWPIRHGIVEDWDLMERFMEQVIFKYLRAEPEDHYFLLTEPPLNTPENREYTAEIMFESFNVPGLYIAVQAVLALAASWTSRQVGERTLTGTVIDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRDITYFIQQLLRDREVGIPPEQSLETAKAVKERYSYVCPDLVKEFNKYDTDGSKWIKQYTGINAISKKEFSIDVGYERFLGPEIFFHPEFANPDFTQPISEVVDEVIQNCPIDVRRPLYKNIVLSGGSTMFRDFGRRLQRDLKRTVDARLKLSEELSGGRLKPKPIDVQVITHHMQRYAVWFGGSMLASTPEFYQVCHTKKDYEEIGPSICRHNPVFGVMS DIP protein P61158 original sequence version: 117 DIP protein P61158 original sequence version: 84 DIP protein P61158 original sequence version: 127 23E5564198B81C63 wasl_human Neural Wiskott-Aldrich syndrome protein WASL protein protein human Homo sapiens Human MSSVQQQPPPPRRVTNVGSLLLTPQENESLFTFLGKKCVTMSSAVVQLYAADRNCMWSKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDPPNGPNLPMATVDIKNPEITTNRFYGPQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRGGPPPPPPPPHNSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPSVAVPPPPPNRMYPPPPPALPSSAPSGPPPPPPSVLGVGPVAPPPPPPPPPPPGPPPPPGLPSDGDHQVPTTAGNKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVADGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDEDDEEDFEDDDEWED DIP protein O00401 original sequence version: 55 DIP protein O00401 original sequence version: 147 DIP protein O00401 original sequence version: 150 DIP protein O00401 original sequence version: 136 DIP protein O00401 original sequence version: 138 DIP protein O00401 original sequence version: 125 DIP protein O00401 original sequence version: 112 DIP protein O00401 original sequence version: 126 B5895C6055E23049 acts_rabit Actin, alpha skeletal muscle ACTA1 ACTA Alpha-actin-1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF DIP protein P68135 original sequence version: 41 DIP protein P68135 original sequence version: 27 DIP protein P68135 original sequence version: 28 DIP protein P68135 original sequence version: 96 DIP protein P68135 original sequence version: 29 DIP protein P68135 original sequence version: 89 DIP protein P68135 original sequence version: 39 DIP protein P68135 original sequence version: 42 DIP protein P68135 original sequence version: 59 DIP protein P68135 original sequence version: 55 DIP protein P68135 original sequence version: 60 DIP protein P68135 original sequence version: 65 DIP protein P68135 original sequence version: 67 DIP protein P68135 original sequence version: 63 DIP protein P68135 original sequence version: 68 DIP protein P68135 original sequence version: 79 DIP protein P68135 original sequence version: 88 DIP protein P68135 original sequence version: 84 DIP protein P68135 original sequence version: 101 DIP protein P68135 original sequence version: 94 DIP protein P68135 original sequence version: 95 DIP protein P68135 original sequence version: 97 DIP protein P68135 original sequence version: 100 DIP protein P68135 original sequence version: 102 DIP protein P68135 original sequence version: 83 DF2A3A046346A179 src8_mouse Src substrate cortactin Cttn Ems1 protein protein mouse Mus musculus Mouse MWKASAGHAVSITQDDGGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRENVFQEHQTLKEKELETGPKASHGYGGKFGVEQDRMDRSAVGHEYQSKLSKHCSQVDSVRGFGGKFGVQMDRVDQSAVGFEYQGKTEKHASQKDYSSGFGGKYGVQADRVDKSAVGFDYQGKTEKHESQKDYSKGFGGKYGIDKDKVDKSAVGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKLQLHESQKDYKTGFGGKFGVQSERQDSSAVGFDYKERLAKHESQQDYAKGFGGKYGVQKDRMDKNASTFEEVVQVPSAYQKTVPIEAVTSKTSNIRANFENLAKEREQEDRRKAEAERAQRMAKERQEQEEARRKLEEQARAKKQTPPASPSPQPIEDRPPSSPIYEDAAPFKAEPSYRGSEPEPEYSIEAAGIPEAGSQQGLTYTSEPVYETTEAPGHYQAEDDTYDGYESDLGITAIALYDYQAAGDDEISFDPDDIITNIEMIDDGWWRGVCKGRYGLFPANYVELRQ DIP protein Q60598 original sequence version: 136 DIP protein Q60598 original sequence version: 78 DIP protein Q60598 original sequence version: 135 8F5CA026ACCD6D4F arp3_bovin Actin-related protein 3 ACTR3 ARP3 Actin-like protein 3 Actin-2 protein protein bovin Bos taurus (Bovine) Bovine MAGRLPACVVDCGTGYTKLGYAGNTEPQFIIPSCIAIKESAKVGDQAQRRVMKGVDDLDFFIGDEAIEKPTYATKWPIRHGIVEDWDLMERFMEQVIFKYLRAEPEDHYFLLTEPPLNTPENREYTAEIMFESFNVPGLYIAVQAVLALAASWTSRQVGERTLTGTVIDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRDITYFIQQLLRDREVGIPPEQSLETAKAVKERYSYVCPDLVKEFNKYDTDGSKWIKQYTGINAISKKEFSIDVGYERFLGPEIFFHPEFANPDFTQPISEVVDEVIQNCPIDVRRPLYKNIVLSGGSTMFRDFGRRLQRDLKRTVDARLKLSEELSGGRLKPKPIDVQVITHHMQRYAVWFGGSMLASTPEFYQVCHTKKDYEEIGPSICRHNPVFGVMS DIP protein P61157 original sequence version: 44 DIP protein P61157 original sequence version: 74 DIP protein P61157 original sequence version: 86 23E5564198B81C63 src8_human Src substrate cortactin CTTN EMS1 Amplaxin Oncogene EMS1 protein protein human Homo sapiens Human MWKASAGHAVSIAQDDAGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRENVFQEHQTLKEKELETGPKASHGYGGKFGVEQDRMDKSAVGHEYQSKLSKHCSQVDSVRGFGGKFGVQMDRVDQSAVGFEYQGKTEKHASQKDYSSGFGGKYGVQADRVDKSAVGFDYQGKTEKHESQRDYSKGFGGKYGIDKDKVDKSAVGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKLQLHESQKDYKTGFGGKFGVQSERQDSAAVGFDYKEKLAKHESQQDYSKGFGGKYGVQKDRMDKNASTFEDVTQVSSAYQKTVPVEAVTSKTSNIRANFENLAKEKEQEDRRKAEAERAQRMAKERQEQEEARRKLEEQARAKTQTPPVSPAPQPTEERLPSSPVYEDAASFKAELSYRGPVSGTEPEPVYSMEAADYREASSQQGLAYATEAVYESAEAPGHYPAEDSTYDEYENDLGITAVALYDYQAAGDDEISFDPDDIITNIEMIDDGWWRGVCKGRYGLFPANYVELRQ DIP protein Q14247 original sequence version: 148 7799326C2B4383BB cttn-actr3-8 2 9 unspecified role unspecified role prey prey bovin-brain Bos taurus brain brain Brain [cerebrum] 8 unspecified role unspecified role bait bait Q60598:p.Asp20_Asp21delinsGlyAla mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain DD GA Thu Jul 07 12:16:36 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was asp20gly, asp21ala Fri Jul 22 10:53:09 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'asp20gly,asp21ala' Wed May 02 17:59:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_asp20-21gly_ala' Tue Jun 30 17:17:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp20_Asp21delinsGlyAla' Q60598:p.Trp22Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A Wed May 02 17:05:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp22ala' Tue Jun 30 16:06:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp22Ala' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Fig 1c. wasl-actr3-1 2 9 unspecified role unspecified role prey prey bovin-brain Bos taurus brain brain Brain [cerebrum] 6 unspecified role unspecified role bait bait vca region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The amino acid range for the VCA region traced from references [PMID:10491394]. region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig 1d. Even though the immunoblot of the Arp3-bound N-WASP was not shown in the paper, the interaction (binding affinities) was demonstrated by quantitating the Arp3 immunoblotted in the supernatant and thus calculating the percentage of Arp3 depletion due to binding to N-WASP . acta1-wasl 2 6 unspecified role unspecified role bait bait vca region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The amino acid range for the VCA region traced from references [PMID:10491394]. region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey rabit Oryctolagus cuniculus (Rabbit) Rabbit physical association physical association Fig 2b. actr3-cttn 3 5 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 5a and 6c. actr3-cttn-1 1 8 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 5a. actr3-cttn-2 3 8 unspecified role unspecified role neutral component neutral component n-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 5c, 6b and 6c. cttn-actr3-12 2 9 unspecified role unspecified role prey prey bovin-brain Bos taurus brain brain Brain [cerebrum] 8 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Fig 1d. The binding affinities of Arp3 for cortactin was measured by immunoblotting the supernatants for Arp3. The quantity of Arp3 immunoblotted in the supernatant is inversely proportional to the amount of cortactin-bound Arp3 in the pellet.