mbinfo MBInfo http://www.mechanobio.info/ The polarization defect of Wiskott-Aldrich syndrome macrophages is linked to dislocalization of the Arp2/3 complex. The polarization defect of Wiskott-Aldrich syndrome macrophages is linked to dislocalization of the Arp2/3 complex. J. Immunol. (0022-1767) 2000 imex curation Linder S., Higgs H., Hufner K., Schwarz K., Pannicke U., Aepfelbacher M. Interactions between WASP-VC and actin (Fig 3B) could not be curated since the specific isoform of actin was not specified and could not be identified. Interaction detection : GST pull down. The source of p41-Arc (endogenous) inferred from the cell lysates (human macrophages) used. The source of WASP inferred from the amino acid length of the constructs. stefan. linder@klp.med.uni-muenchen.de 15-Nov-2012: Contacted by pporras. Only protein-protein interactions human-macrophage Human macrophages macrophage Macrophage fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Linder S., Higgs H., Hufner K., Schwarz K., Pannicke U., Aepfelbacher M. Interactions between WASP-VC and actin (Fig 3B) could not be curated since the specific isoform of actin was not specified and could not be identified. Interaction detection : GST pull down. The source of p41-Arc (endogenous) inferred from the cell lysates (human macrophages) used. The source of WASP inferred from the amino acid length of the constructs. stefan. linder@klp.med.uni-muenchen.de Accepted 2012-OCT-04 by ORCHARD 15-Nov-2012: Contacted by pporras. J. Immunol. (0022-1767) 2000 Only protein-protein interactions imex curation The polarization defect of Wiskott-Aldrich syndrome macrophages is linked to dislocalization of the Arp2/3 complex. The polarization defect of Wiskott-Aldrich syndrome macrophages is linked to dislocalization of the Arp2/3 complex. J. Immunol. (0022-1767) 2000 imex curation Linder S., Higgs H., Hufner K., Schwarz K., Pannicke U., Aepfelbacher M. Interactions between WASP-VC and actin (Fig 3B) could not be curated since the specific isoform of actin was not specified and could not be identified. Interaction detection : GST pull down. The source of p41-Arc (endogenous) inferred from the cell lysates (human macrophages) used. The source of WASP inferred from the amino acid length of the constructs. stefan. linder@klp.med.uni-muenchen.de 15-Nov-2012: Contacted by pporras. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Linder S., Higgs H., Hufner K., Schwarz K., Pannicke U., Aepfelbacher M. Interactions between WASP-VC and actin (Fig 3B) could not be curated since the specific isoform of actin was not specified and could not be identified. Interaction detection : GST pull down. The source of p41-Arc (endogenous) inferred from the cell lysates (human macrophages) used. The source of WASP inferred from the amino acid length of the constructs. stefan. linder@klp.med.uni-muenchen.de Accepted 2012-OCT-04 by ORCHARD 15-Nov-2012: Contacted by pporras. J. Immunol. (0022-1767) 2000 Only protein-protein interactions imex curation arc1b_human Actin-related protein 2/3 complex subunit 1B Arp2/3 complex 41 kDa subunit p41-ARC ARPC1B ARC41 protein protein human Homo sapiens Human MAYHSFLVEPISCHAWNKDRTQIAICPNNHEVHIYEKSGAKWTKVHELKEHNGQVTGIDWAPESNRIVTCGTDRNAYVWTLKGRTWKPTLVILRINRAARCVRWAPNENKFAVGSGSRVISICYFEQENDWWVCKHIKKPIRSTVLSLDWHPNNVLLAAGSCDFKCRIFSAYIKEVEERPAPTPWGSKMPFGELMFESSSSCGWVHGVCFSASGSRVAWVSHDSTVCLADADKKMAVATLASETLPLLALTFITDNSLVAAGHDCFPVLFTYDAAAGMLSFGGRLDVPKQSSQRGLTARERFQNLDKKASSEGGTAAGAGLDSLHKNSVSQISVLSGGKAKCSQFCTTGMDGGMSIWDVKSLESALKDLKIK DIP protein O15143 original sequence version: 110 DIP protein O15143 original sequence version: 150 1939F5B63D40BD27 wasp_human Wiskott-Aldrich syndrome protein WAS IMD2 protein protein human Homo sapiens Human MSGGPMGGRPGGRGAPAVQQNIPSTLLQDHENQRLFEMLGRKCLTLATAVVQLYLALPPGAEHWTKEHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYSTPTPFFHTFAGDDCQAGLNFADEDEAQAFRALVQEKIQKRNQRQSGDRRQLPPPPTPANEERRGGLPPLPLHPGGDQGGPPVGPLSLGLATVDIQNPDITSSRYRGLPAPGPSPADKKRSGKKKISKADIGAPSGFKHVSHVGWDPQNGFDVNNLDPDLRSLFSRAGISEAQLTDAETSKLIYDFIEDQGGLEAVRQEMRRQEPLPPPPPPSRGGNQLPRPPIVGGNKGRSGPLPPVPLGIAPPPPTPRGPPPPGRGGPPPPPPPATGRSGPLPPPPPGAGGPPMPPPPPPPPPPPSSGNGPAPPPLPPALVPAGGLAPGGGRGALLDQIRQGIQLNKTPGAPESSALQPPPQSSEGLVGALMHVMQKRSRAIHSSDEGEDQAGDEDEDDEWDD DIP protein P42768 original sequence version: 183 DIP protein P42768 original sequence version: 168 DIP protein P42768 original sequence version: 132 DIP protein P42768 original sequence version: 155 7228428672B7CB78 was-arpc1b-1 2 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait acidic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 3B. arpc1b-was 1 4 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 1F.