mbinfo MBInfo http://www.mechanobio.info/ Cooperation between mDia1 and ROCK in Rho-induced actin reorganization. Cooperation between mDia1 and ROCK in Rho-induced actin reorganization. Nat. Cell Biol. (1465-7392) 1999 imex curation Watanabe N., Kato T., Fujita A., Ishizaki T., Narumiya S. Interaction detection: GST pull down. The interactions of RhoA, RhoB, RhoC, Rac1 and Cdc42 with mDia1 (Fig 2c) could not be curated since the source of these GTPases was not specified and could not be traced. 15-Nov-2012: Contacted by pporras. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Watanabe N., Kato T., Fujita A., Ishizaki T., Narumiya S. Interaction detection: GST pull down. The interactions of RhoA, RhoB, RhoC, Rac1 and Cdc42 with mDia1 (Fig 2c) could not be curated since the source of these GTPases was not specified and could not be traced. Accepted 2012-NOV-05 by ORCHARD 15-Nov-2012: Contacted by pporras. Nat. Cell Biol. (1465-7392) 1999 Only protein-protein interactions imex curation Cooperation between mDia1 and ROCK in Rho-induced actin reorganization. Cooperation between mDia1 and ROCK in Rho-induced actin reorganization. Nat. Cell Biol. (1465-7392) 1999 imex curation Watanabe N., Kato T., Fujita A., Ishizaki T., Narumiya S. Interaction detection: GST pull down. The interactions of RhoA, RhoB, RhoC, Rac1 and Cdc42 with mDia1 (Fig 2c) could not be curated since the source of these GTPases was not specified and could not be traced. 15-Nov-2012: Contacted by pporras. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Watanabe N., Kato T., Fujita A., Ishizaki T., Narumiya S. Interaction detection: GST pull down. The interactions of RhoA, RhoB, RhoC, Rac1 and Cdc42 with mDia1 (Fig 2c) could not be curated since the source of these GTPases was not specified and could not be traced. Accepted 2012-NOV-05 by ORCHARD 15-Nov-2012: Contacted by pporras. Nat. Cell Biol. (1465-7392) 1999 Only protein-protein interactions imex curation diap1_mouse Protein diaphanous homolog 1 Diaphanous-related formin-1 p140mDIA Diaph1 Diap1 protein protein mouse Mus musculus Mouse MEPSGGGLGPGRGTRDKKKGRSPDELPATGGDGGKHKKFLERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDISDEQVLVLFEQMLVDMNLNEEKQQPLREKDIVIKREMVSQYLHTSKAGMNQKESSRSAMMYIQELRSGLRDMHLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETSGNYDSRNQHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQELREIENEDMKVQLCVFDEQGDEDFFDLKGRLDDIRMEMDDFGEVFQIILNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECVSQIVLHKNGTDPDFKCRHLQIDIERLVDQMIDKTKVEKSEAKATELEKKLDSELTARHELQVEMKKMENDFEQKLQDLQGEKDALDSEKQQITAQKQDLEAEVSKLTGEVAKLSKELEDAKNEMASLSAVVVAPSVSSSAAVPPAPPLPGDSGTVIPPPPPPPPLPGGVVPPSPPLPPGTCIPPPPPLPGGACIPPPPQLPGSAAIPPPPPLPGVASIPPPPPLPGATAIPPPPPLPGATAIPPPPPLPGGTGIPPPPPPLPGSVGVPPPPPLPGGPGLPPPPPPFPGAPGIPPPPPGMGVPPPPPFGFGVPAAPVLPFGLTPKKVYKPEVQLRRPNWSKFVAEDLSQDCFWTKVKEDRFENNELFAKLTLAFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKEEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSENFSSLLELTLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSADQKMTLLHFLAELCENDHPEVLKFPDELAHVEKASRVSAENLQKSLDQMKKQIADVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGDYFVFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQVNRKAGCAVTSLLASELTKDDAMAPGPVKVPKKSEGVPTILEEAKELVGRAS DIP protein O08808 original sequence version: 62 DIP protein O08808 original sequence version: 141 09404164873CA7C1 diaph1-diaph1-3 2 3 unspecified role unspecified role prey prey drai uncategorized protein modification uncategorized protein modification undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined The mDia1-N-terminal fragment was tagged with the Thio-His fusion protein. n-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role bait bait c-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 9 physical association physical association Fig 2c, 2d. The binding of the N-terminal mDia1 to C-terminal mDia1 was disrupted in the presence of GTPgammaS-loaded RhoA and was decreased in the presence of GDP-loaded RhoA. The interaction was not affected in the presence of Rac1 and Cdc42. (Fig 2d). diaph1-diaph1-5 1 3 unspecified role unspecified role bait bait n-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain This N-terminal region is contained within the [delta Pst ] which includes the amino acids [1-449 and 1190-1255]. 3 unspecified role unspecified role prey prey c-terminal region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 13 15 physical association physical association Fig 2b. diaph1-diaph1-7 1 3 unspecified role unspecified role bait bait c-terminal region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey n-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain This N-terminal region is contained in the [delta Pst] mutant which includes the amino acids [1-449 and 1190-1255]. 20 18 physical association physical association Fig 2b.