MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url&gt;http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Catalytically active filaments - pyruvate decarboxylase from Neurospora crassa. pH-controlled oligomer structure and catalytic function. Catalytically active filaments - pyruvate decarboxylase from Neurospora crassa. pH-controlled oligomer structure and catalytic function. FEBS J. (1742-464X) 2012 imex curation Hüttl S., Fiebig J., Kutter S., Hause G., Lilie H., Spinka M., König S. Only protein-protein interactions in vitro In vitro light scattering light scattering predetermined predetermined participant predetermined Hüttl S., Fiebig J., Kutter S., Hause G., Lilie H., Spinka M., König S. FEBS J. (1742-464X) 2012 Only protein-protein interactions imex curation Catalytically active filaments - pyruvate decarboxylase from Neurospora crassa. pH-controlled oligomer structure and catalytic function. Catalytically active filaments - pyruvate decarboxylase from Neurospora crassa. pH-controlled oligomer structure and catalytic function. FEBS J. (1742-464X) 2012 imex curation Hüttl S., Fiebig J., Kutter S., Hause G., Lilie H., Spinka M., König S. Only protein-protein interactions in vitro In vitro electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined Hüttl S., Fiebig J., Kutter S., Hause G., Lilie H., Spinka M., König S. FEBS J. (1742-464X) 2012 Only protein-protein interactions imex curation Catalytically active filaments - pyruvate decarboxylase from Neurospora crassa. pH-controlled oligomer structure and catalytic function. Catalytically active filaments - pyruvate decarboxylase from Neurospora crassa. pH-controlled oligomer structure and catalytic function. FEBS J. (1742-464X) 2012 imex curation Hüttl S., Fiebig J., Kutter S., Hause G., Lilie H., Spinka M., König S. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Hüttl S., Fiebig J., Kutter S., Hause G., Lilie H., Spinka M., König S. FEBS J. (1742-464X) 2012 Only protein-protein interactions imex curation pdc_neucr Pyruvate decarboxylase cfp 8-10 nm cytoplasmic filament-associated protein P59NC pdc-1 NCU02193 protein protein neucr Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) MVAQQQGKFTVGDYLAERLAQVGVRHHFVVPGDYNLILLDKLQAHPDLKEVGCANELNCSLAAEGYARANGISACVVTYSVGALSAFNGTGSAYAENLPLVLISGSPNTNDPSQYHILHHTLGHPDYTYQYEMAKKITCCAVAIPRAIDAPRLIDRALRAAILARKPCYIEIPTNLAGATCVRPGPISAITDPITSDKSALEAAAKCAAEYLDGKLKPVILVGPKAGRAGSEKELIEFAEAMGCAVALQPAAKGMFPEDHKQFVGIFWGQVSSDAADAMVHWADAMICVGAVFNDYSTVGWTAVPNIPLMTVDMDHVTFPGAHFSRVRMCEFLSHLATQVTFNDSTMIEYKRLKPDPPHVHTAEREEPLSRKEISRQVQEMLTDKTSLFVDTGDSWFNGIQLKLPPGAKFEIEMQWGHIGWSIPAAFGYALRHPDRHTIVLVGDGSFQVTAQEVSQMVRFKVPITIMLINNRGYTIEVEIHDGSYNKIKNWDYAMLVEAFNSTDGHAKGLLANTAGELADAIKVAESHKEGPTLIECTIDQDDCSKELITWGHYVAAANARPPRNMSVQE mint DF48B86C24EDAACF cfp-1 3 PDC 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 4.0 direct interaction direct interaction sf4 mint Analytical ultracentrifugation of NcPDC solutions yields sedimentation coefficients of 10.4 S at pH 7.5 and 12.6 S at pH 7.0 (figure S4). A sedimentation coefficient of 10 S is typical for the tetrameric state of PDC [21], while the higher value of 12.6 S at pH 7.0 points to a tetramer-octamer equilibrium. These results confirm the conclusions on pH dependent oligomeric equilibria in NcPDC drawn already from the other structural methods. cfp-1 3 PDC 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 8.0 direct interaction direct interaction sf4 mint Analytical ultracentrifugation of NcPDC solutions yields sedimentation coefficients of 10.4 S at pH 7.5 and 12.6 S at pH 7.0 (figure S4). A sedimentation coefficient of 10 S is typical for the tetrameric state of PDC [21], while the higher value of 12.6 S at pH 7.0 points to a tetramer-octamer equilibrium. These results confirm the conclusions on pH dependent oligomeric equilibria in NcPDC drawn already from the other structural methods. cfp-1 1 PDC 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 6.0 direct interaction direct interaction f3 mint Molecular masses calculated from the ratio of the scattering intensities of aqueous solutions of NcPDC and bovine serum albumin, respectively, point to hexamers and even to dodecamers. Even at pH 7.0 a certain tendency to form oligomer structures larger than tetramers is obvious from the scattering patterns at s<0.2 nm-1 (figure 3). cfp-1 1 PDC 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 12.0 direct interaction direct interaction f3 mint Molecular masses calculated from the ratio of the scattering intensities of aqueous solutions of NcPDC and bovine serum albumin, respectively, point to hexamers and even to dodecamers. Even at pH 7.0 a certain tendency to form oligomer structures larger than tetramers is obvious from the scattering patterns at s<0.2 nm-1 (figure 3). cfp-cfp 2 PDC 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified PDC 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction mint f4 sf5 Finally, electron micrographs of NcPDC stained negatively with uranyl acetate clearly document the existence of bundles of filaments at pH 5.0 and 5.5 at magnifications of 30,000-85,000. Individual filaments are 120-200 nm long and possess diameters between 7 and 10 nm. A number of filaments are typically arranged side by side within the bundles. Strikingly, this type of superstructure is totally absent at pH 6.5 (figure 4).