InnateDB InnateDB Indoleamine 2,3-dioxygenase is a signaling protein in long-term tolerance by dendritic cells. Indoleamine 2,3-dioxygenase is a signaling protein in long-term tolerance by dendritic cells. Nat. Immunol. (1529-2908) 2011 imex curation Pallotta M.T., Orabona C., Volpi C., Vacca C., Belladonna M.L., Bianchi R., Servillo G., Brunacci C., Calvitti M., Bicciato S., Mazza E.M., Boon L., Grassi F., Fioretti M.C., Fallarino F., Puccetti P., Grohmann U. 30/01/12:- I have finished your corrections for you as I very much wanted to get this paper out of my "checking" area - however you still have ownership of the publication so I cannot accept it. When you return it to me I wil get it accepted. Please let me know if/when you have finished the other publications outstanding... Murine P1 tumor cells with high constitutive expression of phosphatases were used in this experiment; - the exact cell line is not stated. ugrohmann@tin.it 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions mouse-pdc Mouse-Plasmacytoid Dendritic Cells plasmacytoid_dendritic_cell Plasmacytoid Dendritic Cell Look like plasma cells, but have certain characteristics similar to myeloid dendritic cells. They constitute < 0.4% of peripheral blood mononuclear cells (PBMC). In humans these cells express the surface markers CD123, BDCA-2(CD303) and BDCA-4(CD304), but do not express CD11c or CD14, which distinguishes them from conventional dendritic cells or monocytes, respectively. These cells express intracellular Toll-like receptors 7 and 9 which detect ssRNA and CpG DNA motifs, respectively. Upon stimulation and subsequent activation, these cells produce large amounts of type I interferon (mainly IFN-α (alpha) and IFN-β (beta). anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Pallotta M.T., Orabona C., Volpi C., Vacca C., Belladonna M.L., Bianchi R., Servillo G., Brunacci C., Calvitti M., Bicciato S., Mazza E.M., Boon L., Grassi F., Fioretti M.C., Fallarino F., Puccetti P., Grohmann U. 30/01/12:- I have finished your corrections for you as I very much wanted to get this paper out of my "checking" area - however you still have ownership of the publication so I cannot accept it. When you return it to me I wil get it accepted. Please let me know if/when you have finished the other publications outstanding... Murine P1 tumor cells with high constitutive expression of phosphatases were used in this experiment; - the exact cell line is not stated. ugrohmann@tin.it Accepted 2012-JUL-31 by MGT 19-Sept-2012: Contacted by IntAct-Help. Nat. Immunol. (1529-2908) 2011 Only protein-protein interactions imex curation Indoleamine 2,3-dioxygenase is a signaling protein in long-term tolerance by dendritic cells. Indoleamine 2,3-dioxygenase is a signaling protein in long-term tolerance by dendritic cells. Nat. Immunol. (1529-2908) 2011 imex curation Pallotta M.T., Orabona C., Volpi C., Vacca C., Belladonna M.L., Bianchi R., Servillo G., Brunacci C., Calvitti M., Bicciato S., Mazza E.M., Boon L., Grassi F., Fioretti M.C., Fallarino F., Puccetti P., Grohmann U. 30/01/12:- I have finished your corrections for you as I very much wanted to get this paper out of my "checking" area - however you still have ownership of the publication so I cannot accept it. When you return it to me I wil get it accepted. Please let me know if/when you have finished the other publications outstanding... Murine P1 tumor cells with high constitutive expression of phosphatases were used in this experiment; - the exact cell line is not stated. ugrohmann@tin.it 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions mouse Mus musculus Mouse fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Pallotta M.T., Orabona C., Volpi C., Vacca C., Belladonna M.L., Bianchi R., Servillo G., Brunacci C., Calvitti M., Bicciato S., Mazza E.M., Boon L., Grassi F., Fioretti M.C., Fallarino F., Puccetti P., Grohmann U. 30/01/12:- I have finished your corrections for you as I very much wanted to get this paper out of my "checking" area - however you still have ownership of the publication so I cannot accept it. When you return it to me I wil get it accepted. Please let me know if/when you have finished the other publications outstanding... Murine P1 tumor cells with high constitutive expression of phosphatases were used in this experiment; - the exact cell line is not stated. ugrohmann@tin.it Accepted 2012-JUL-31 by MGT 19-Sept-2012: Contacted by IntAct-Help. Nat. Immunol. (1529-2908) 2011 Only protein-protein interactions imex curation Indoleamine 2,3-dioxygenase is a signaling protein in long-term tolerance by dendritic cells. Indoleamine 2,3-dioxygenase is a signaling protein in long-term tolerance by dendritic cells. Nat. Immunol. (1529-2908) 2011 imex curation Pallotta M.T., Orabona C., Volpi C., Vacca C., Belladonna M.L., Bianchi R., Servillo G., Brunacci C., Calvitti M., Bicciato S., Mazza E.M., Boon L., Grassi F., Fioretti M.C., Fallarino F., Puccetti P., Grohmann U. 30/01/12:- I have finished your corrections for you as I very much wanted to get this paper out of my "checking" area - however you still have ownership of the publication so I cannot accept it. When you return it to me I wil get it accepted. Please let me know if/when you have finished the other publications outstanding... Murine P1 tumor cells with high constitutive expression of phosphatases were used in this experiment; - the exact cell line is not stated. ugrohmann@tin.it 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions mouse-pdc Mouse-Plasmacytoid Dendritic Cells plasmacytoid_dendritic_cell Plasmacytoid Dendritic Cell Look like plasma cells, but have certain characteristics similar to myeloid dendritic cells. They constitute < 0.4% of peripheral blood mononuclear cells (PBMC). In humans these cells express the surface markers CD123, BDCA-2(CD303) and BDCA-4(CD304), but do not express CD11c or CD14, which distinguishes them from conventional dendritic cells or monocytes, respectively. These cells express intracellular Toll-like receptors 7 and 9 which detect ssRNA and CpG DNA motifs, respectively. Upon stimulation and subsequent activation, these cells produce large amounts of type I interferon (mainly IFN-α (alpha) and IFN-β (beta). pull down pull down western blot western blot Immuno blot Immuno blot Pallotta M.T., Orabona C., Volpi C., Vacca C., Belladonna M.L., Bianchi R., Servillo G., Brunacci C., Calvitti M., Bicciato S., Mazza E.M., Boon L., Grassi F., Fioretti M.C., Fallarino F., Puccetti P., Grohmann U. 30/01/12:- I have finished your corrections for you as I very much wanted to get this paper out of my "checking" area - however you still have ownership of the publication so I cannot accept it. When you return it to me I wil get it accepted. Please let me know if/when you have finished the other publications outstanding... Murine P1 tumor cells with high constitutive expression of phosphatases were used in this experiment; - the exact cell line is not stated. ugrohmann@tin.it Accepted 2012-JUL-31 by MGT 19-Sept-2012: Contacted by IntAct-Help. Nat. Immunol. (1529-2908) 2011 Only protein-protein interactions imex curation Indoleamine 2,3-dioxygenase is a signaling protein in long-term tolerance by dendritic cells. Indoleamine 2,3-dioxygenase is a signaling protein in long-term tolerance by dendritic cells. Nat. Immunol. (1529-2908) 2011 imex curation Pallotta M.T., Orabona C., Volpi C., Vacca C., Belladonna M.L., Bianchi R., Servillo G., Brunacci C., Calvitti M., Bicciato S., Mazza E.M., Boon L., Grassi F., Fioretti M.C., Fallarino F., Puccetti P., Grohmann U. 30/01/12:- I have finished your corrections for you as I very much wanted to get this paper out of my "checking" area - however you still have ownership of the publication so I cannot accept it. When you return it to me I wil get it accepted. Please let me know if/when you have finished the other publications outstanding... Murine P1 tumor cells with high constitutive expression of phosphatases were used in this experiment; - the exact cell line is not stated. ugrohmann@tin.it 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions mouse Mus musculus Mouse pull down pull down western blot western blot Immuno blot Immuno blot Pallotta M.T., Orabona C., Volpi C., Vacca C., Belladonna M.L., Bianchi R., Servillo G., Brunacci C., Calvitti M., Bicciato S., Mazza E.M., Boon L., Grassi F., Fioretti M.C., Fallarino F., Puccetti P., Grohmann U. 30/01/12:- I have finished your corrections for you as I very much wanted to get this paper out of my "checking" area - however you still have ownership of the publication so I cannot accept it. When you return it to me I wil get it accepted. Please let me know if/when you have finished the other publications outstanding... Murine P1 tumor cells with high constitutive expression of phosphatases were used in this experiment; - the exact cell line is not stated. ugrohmann@tin.it Accepted 2012-JUL-31 by MGT 19-Sept-2012: Contacted by IntAct-Help. Nat. Immunol. (1529-2908) 2011 Only protein-protein interactions imex curation ptn6_mouse Tyrosine-protein phosphatase non-receptor type 6 Ptpn6 Hcp Hcph Ptp1C Protein-tyrosine phosphatase 1C Hematopoietic cell protein-tyrosine phosphatase 70Z-SHP SH-PTP1 PTPTY-42 protein protein mouse Mus musculus Mouse MVRWFHRDLSGPDAETLLKGRGVPGSFLARPSRKNQGDFSLSVRVDDQVTHIRIQNSGDFYDLYGGEKFATLTELVEYYTQQQGILQDRDGTIIHLKYPLNCSDPTSERWYHGHISGGQAESLLQAKGEPWTFLVRESLSQPGDFVLSVLNDQPKAGPGSPLRVTHIKVMCEGGRYTVGGSETFDSLTDLVEHFKKTGIEEASGAFVYLRQPYYATRVNAADIENRVLELNKKQESEDTAKAGFWEEFESLQKQEVKNLHQRLEGQRPENKSKNRYKNILPFDHSRVILQGRDSNIPGSDYINANYVKNQLLGPDENSKTYIASQGCLDATVNDFWQMAWQENTRVIVMTTREVEKGRNKCVPYWPEVGTQRVYGLYSVTNSREHDTAEYKLRTLQISPLDNGDLVREIWHYQYLSWPDHGVPSEPGGVLSFLDQINQRQESLPHAGPIIVHCSAGIGRTGTIIVIDMLMESISTKGLDCDIDIQKTIQMVRAQRSGMVQTEAQYKFIYVAIAQFIETTKKKLEIIQSQKGQESEYGNITYPPAVRSAHAKASRTSSKHKEEVYENVHSKSKKEEKVKKQRSADKEKNKGSLKRK DIP protein P29351 original sequence version: 154 DIP protein P29351 original sequence version: 102 DIP protein P29351 original sequence version: 117 DIP protein P29351 original sequence version: 164 DIP protein P29351 original sequence version: 134 CF17300D032638D2 ptn11_mouse Tyrosine-protein phosphatase non-receptor type 11 Ptpn11 Protein-tyrosine phosphatase SYP SH-PTP2 protein protein mouse Mus musculus Mouse MTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIVDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDCDIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRRIEEEQKSKRKGHEYTNIKYSLVDQTSGDQSPLPPCTPTPPCAEMREDSARVYENVGLMQQQRSFR DIP protein P35235 original sequence version: 93 DIP protein P35235 original sequence version: 99 DIP protein P35235 original sequence version: 138 6C71EEEECCF7F159 i23o1_mouse Indoleamine 2,3-dioxygenase 1 Ido1 Ido Indo Indoleamine-pyrrole 2,3-dioxygenase protein protein mouse Mus musculus Mouse MALSKISPTEGSRRILEDHHIDEDVGFALPHPLVELPDAYSPWVLVARNLPVLIENGQLREEVEKLPTLSTDGLRGHRLQRLAHLALGYITMAYVWNRGDDDVRKVLPRNIAVPYCELSEKLGLPPILSYADCVLANWKKKDPNGPMTYENMDILFSFPGGDCDKGFFLVSLLVEIAASPAIKAIPTVSSAVERQDLKALEKALHDIATSLEKAKEIFKRMRDFVDPDTFFHVLRIYLSGWKCSSKLPEGLLYEGVWDTPKMFSGGSAGQSSIFQSLDVLLGIKHEAGKESPAEFLQEMREYMPPAHRNFLFFLESAPPVREFVISRHNEDLTKAYNECVNGLVSVRKFHLAIVDTYIMKPSKKKPTDGDKSEEPSNVESRGTGGTNPMTFLRSVKDTTEKALLSWP DIP protein P28776 original sequence version: 79 317681EABF96A727 ido1-ptpn11-5 4 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain p_tyr_115 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey physical association physical association Fig. 3C ido1-ptpn11-3 4 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p_tyr_253 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 3C ido1-ptpn11-6 3 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait ptyr253 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3C TGF-beta ido1-ptpn11-7 3 7 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ptyr115 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey physical association physical association Fig. 3C TGF-beta ido1-ptpn11-8 1 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey association association Fig. 3D TGF-B ido1-ptpn6-4 2 5 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component P28776:p.[Tyr115Phe;Tyr253Phe] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 19:08:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr115phe,tyr253phe' Tue Jun 30 16:53:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr115Phe;Tyr253Phe]' colocalization colocalization Fig. 3G