IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The inside-out mechanism of dicers from budding yeasts. The inside-out mechanism of dicers from budding yeasts. Cell (0092-8674) 2011 imex curation Weinberg D.E., Nakanishi K., Patel D.J., Bartel D.P. RNA sequences not given so RNA binding assays not captured. pateld@mskcc.org, dbartel@wi.mit.edu 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Weinberg D.E., Nakanishi K., Patel D.J., Bartel D.P. RNA sequences not given so RNA binding assays not captured. pateld@mskcc.org, dbartel@wi.mit.edu Accepted 2011-AUG-31 by MGT 23-Sept-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation The inside-out mechanism of dicers from budding yeasts. The inside-out mechanism of dicers from budding yeasts. Cell (0092-8674) 2011 imex curation Weinberg D.E., Nakanishi K., Patel D.J., Bartel D.P. RNA sequences not given so RNA binding assays not captured. pateld@mskcc.org, dbartel@wi.mit.edu 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Weinberg D.E., Nakanishi K., Patel D.J., Bartel D.P. RNA sequences not given so RNA binding assays not captured. pateld@mskcc.org, dbartel@wi.mit.edu Accepted 2011-AUG-31 by MGT 23-Sept-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation The inside-out mechanism of dicers from budding yeasts. The inside-out mechanism of dicers from budding yeasts. Cell (0092-8674) 2011 imex curation Weinberg D.E., Nakanishi K., Patel D.J., Bartel D.P. RNA sequences not given so RNA binding assays not captured. pateld@mskcc.org, dbartel@wi.mit.edu 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight silver stain molecular weight estimation by silver staining weight silver stain Weinberg D.E., Nakanishi K., Patel D.J., Bartel D.P. RNA sequences not given so RNA binding assays not captured. Cross-linking agent - glutaraldehyde pateld@mskcc.org, dbartel@wi.mit.edu Accepted 2011-AUG-31 by MGT 23-Sept-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation I''ve shortened the construct to encompass the deltaN (with a bit of help from InterPro) - I don't see the problem in merging the different figures to the shortest sufficient to bind region so have left the figs using the longer construct as part of this interaction. The inside-out mechanism of dicers from budding yeasts. The inside-out mechanism of dicers from budding yeasts. Cell (0092-8674) 2011 imex curation Weinberg D.E., Nakanishi K., Patel D.J., Bartel D.P. RNA sequences not given so RNA binding assays not captured. pateld@mskcc.org, dbartel@wi.mit.edu 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions in vitro In vitro light scattering light scattering predetermined predetermined participant predetermined Weinberg D.E., Nakanishi K., Patel D.J., Bartel D.P. RNA sequences not given so RNA binding assays not captured. pateld@mskcc.org, dbartel@wi.mit.edu Accepted 2011-AUG-31 by MGT 23-Sept-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation a7tr32_vanpo Kpol_455p11 protein protein vanpo Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) MSDQVYVPPTSAPMSNELKVREFYRLHNACVKLKESIKLIYENPLVTDQNVLNLGTAENTIDYTILNTPTLNVAKTLLGNRYSLDLIDLFQSHDFKDSNTDVDMFIKYPVVYDENLENLAFMHKSFPNMNAHLNDAQKTQLSNERLEFLGDSWLGALVSYIVYTRFPSANEGMLSQMKESIVNNNNLFDWSTKLNFTKRLQGNIATPTRVVKDKMSKRYADCVEAYIGALVIDRFGTEFLDIKEWLEELSEKKLAKSSQMVIKEPLNKNAKNELAELLQINKLGHKLHYRKLTEMPPFRVEVKIGDILLDEAEGNSIREAEHRAAMKVLENDELLRKYSVYDLEDGNSKKYSIEETDLKHDIESSGISNENFLQNEGNGTENIESKFKQLNISNDSNKDDLVNNITNSVFSKLQDVVSKIVIDAISESIPNNPHKIEQSRNGKVGTQTNTSATTSDVLSVQTSIPKSESTSADKAPITCDPNAKQALYTLLSRSQVFPDYEMVQHSMDNFTANCIIKGSGIFLASGRGRSKKIAQQNAASAALRSPILKSILEEQNNF 86DA95077ECA2886 dcr1-dcr1 1 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 2.29A Working 17.74%, Free:21.97% Figure 2A, Table S2 dcr1-dcr1-1 2 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. Figure 2A dcr1-dcr1-2 4 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. Figure 2B dcr1-dcr1-3 3 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 4F, Supp. Figures 2C, 2F The coordinates of the delta N construct have not been given in the publication so have been inferred from Fig. 1A and InterPro annotation. a7tr32 1 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain A7TR32:p.Glu224Gln mutation mutation certain certain sequence position certain certain certain sequence position certain E Q Wed May 02 19:47:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu224gln' Tue Jun 30 17:32:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu224Gln' ecoli Escherichia coli (strain K12) direct interaction direct interaction 1.98A Working 19.65%, Free:23.59% Figure 2C, Table S2