MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Cellular vacuolation induced by Clostridium perfringens epsilon-toxin. Cellular vacuolation induced by Clostridium perfringens epsilon-toxin. FEBS J. (1742-464X) 2011 imex curation Nagahama M., Itohayashi Y., Hara H., Higashihara M., Fukatani Y., Takagishi T., Oda M., Kobayashi K., Nakagawa I., Sakurai J. nagahama@ph.bunri-u.ac.jp Only protein-protein interactions in vitro In vitro density sedimentation cosedimentation through density gradient density sedimentation weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Nagahama M., Itohayashi Y., Hara H., Higashihara M., Fukatani Y., Takagishi T., Oda M., Kobayashi K., Nakagawa I., Sakurai J. nagahama@ph.bunri-u.ac.jp FEBS J. (1742-464X) 2011 Only protein-protein interactions imex curation e7d8r1_clopf etx protein protein clostridium perfringens d Clostridium perfringens D MKKNLVKSLAIASAVISIYSIVNIVSPTNVIAKEISNTVSNEMSKKASYDNVDTLIEKGRYNTKYNYLKRMEKYYPNAMAYFDKVTINPQGNDFYINNPKVELDGEPSMNYLEDVYVGKALLTNDTQQEQKLKSQSFTCKNTDTVTATTTHTVGTSIQATAKFTVPFNETGVSLTTSYSFANTNTNTNSKEITHNVPSQDILVPANTTVEVIAYLKKVNVKGNVKLVGQVSGSEWGEIPSYLAFPRDGYKFSLSDTVNKSDLNEDGTININGKGNYSAVMGDELIVKVRNLNTNNVQEYVIPVDKKEKSNDSNIVKYRSLSIKAPGIKAEAAAK mint 8C80F71E3E30B0B6 etx-etx 1 Epsilon-toxin 2 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep Epsilon-toxin 2 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep colocalization colocalization mint f4a f4b f4c We investigated whether the toxin bound to MDCK cells could be recovered in Triton X-100-insoluble fractions. As shown in Fig. 4A, when the cells were incubated with 125I-epsilon-toxin at 4 °C for 60 min, the monomer (28 kDa) of the toxin was found in the soluble fractions (no. 7-10). We first showed that the monomer binds to the triton-soluble fraction at 4 °C. When MDCK cells preincubated with 125118 I-epsilon-toxin at 4 °C for 60 min were incubated at 37 °C for 240 min (Fig. 4B), the insoluble fractions (no. 3-5) were found to contain an oligomer (about 180 kDa). As shown in Fig. 4C, oligomer formation of the toxin began after 15 min of incubation at 37 °C and reached a maximum after 60 min of incubation.