IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Comparative analysis of fluorescence resonance energy transfer (FRET) and proximity ligation assay (PLA). Comparative analysis of fluorescence resonance energy transfer (FRET) and proximity ligation assay (PLA). Proteomics (1615-9853) 2011 imex curation Mocanu M.M., Varadi T., Szollosi J., Nagy P. The authors conclude that due to saturation effects, PLA is recommended as a semi-quantitative tool for measuring protein associations. correction - comment from Expt-4:- I am not sure what you want me to do about this:-" Also Fig. 2 only. In all other cases they are using Abs to different epitopes in the same molecule so measuring intramolecular associations not inter." Do you want me to enter another interaction with two interactors and stoich = 0, rather than 2? (entered below= I have not entered a Biological Role as either Self or Putative self, as some Figs are showing intermolecular binding, and others intra-mol binding.) Fig 3:- cannot find a cell line, so not entered anywhere. I have not entered a Biological Role as either Self or Putative self, as some Figs are showing intermolecular binding, and others intra-mol binding. Cells were labelled with anti-trastuzumab, anti-pertuzumab (Cy5 -PLA signal detection) and anti-7C2 (AlexaFluor488) antibodies against ERBB2. nagyp@med.unideb.hu 23-Sept-2011: Contacted by JYOTI. orchard Only protein-protein interactions human-a_431 Homo sapiens epidermoid carcinoma a_431 Human epidermoid carcinoma pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Mocanu M.M., Varadi T., Szollosi J., Nagy P. The authors conclude that due to saturation effects, PLA is recommended as a semi-quantitative tool for measuring protein associations. correction - comment from Expt-4:- I am not sure what you want me to do about this:-" Also Fig. 2 only. In all other cases they are using Abs to different epitopes in the same molecule so measuring intramolecular associations not inter." Do you want me to enter another interaction with two interactors and stoich = 0, rather than 2? (entered below= I have not entered a Biological Role as either Self or Putative self, as some Figs are showing intermolecular binding, and others intra-mol binding.) Cells were labelled with anti-trastuzumab, anti-pertuzumab (Cy5 -PLA signal detection) and anti-7C2 (AlexaFluor488) antibodies against ERBB2. nagyp@med.unideb.hu Accepted 2011-AUG-30 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Proteomics (1615-9853) 2011 orchard Only protein-protein interactions imex curation Comparative analysis of fluorescence resonance energy transfer (FRET) and proximity ligation assay (PLA). Comparative analysis of fluorescence resonance energy transfer (FRET) and proximity ligation assay (PLA). Proteomics (1615-9853) 2011 imex curation Mocanu M.M., Varadi T., Szollosi J., Nagy P. The authors conclude that due to saturation effects, PLA is recommended as a semi-quantitative tool for measuring protein associations. correction - comment from Expt-4:- I am not sure what you want me to do about this:-" Also Fig. 2 only. In all other cases they are using Abs to different epitopes in the same molecule so measuring intramolecular associations not inter." Do you want me to enter another interaction with two interactors and stoich = 0, rather than 2? (entered below= I have not entered a Biological Role as either Self or Putative self, as some Figs are showing intermolecular binding, and others intra-mol binding.) Fig 3:- cannot find a cell line, so not entered anywhere. I have not entered a Biological Role as either Self or Putative self, as some Figs are showing intermolecular binding, and others intra-mol binding. Cells were labelled with anti-trastuzumab, anti-pertuzumab (Cy5 -PLA signal detection) and anti-7C2 (AlexaFluor488) antibodies against ERBB2. nagyp@med.unideb.hu 23-Sept-2011: Contacted by JYOTI. orchard Only protein-protein interactions human-a_431 Homo sapiens epidermoid carcinoma a_431 Human epidermoid carcinoma fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Mocanu M.M., Varadi T., Szollosi J., Nagy P. The authors conclude that due to saturation effects, PLA is recommended as a semi-quantitative tool for measuring protein associations. correction - comment from Expt-4:- I am not sure what you want me to do about this:-" Also Fig. 2 only. In all other cases they are using Abs to different epitopes in the same molecule so measuring intramolecular associations not inter." Do you want me to enter another interaction with two interactors and stoich = 0, rather than 2? (entered below= I have not entered a Biological Role as either Self or Putative self, as some Figs are showing intermolecular binding, and others intra-mol binding.) Cells were labelled with anti-trastuzumab, anti-pertuzumab (Cy5 -PLA signal detection) and anti-7C2 (AlexaFluor488) antibodies against ERBB2. nagyp@med.unideb.hu Accepted 2011-AUG-30 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Proteomics (1615-9853) 2011 orchard Only protein-protein interactions imex curation Comparative analysis of fluorescence resonance energy transfer (FRET) and proximity ligation assay (PLA). Comparative analysis of fluorescence resonance energy transfer (FRET) and proximity ligation assay (PLA). Proteomics (1615-9853) 2011 imex curation Mocanu M.M., Varadi T., Szollosi J., Nagy P. The authors conclude that due to saturation effects, PLA is recommended as a semi-quantitative tool for measuring protein associations. correction - comment from Expt-4:- I am not sure what you want me to do about this:-" Also Fig. 2 only. In all other cases they are using Abs to different epitopes in the same molecule so measuring intramolecular associations not inter." Do you want me to enter another interaction with two interactors and stoich = 0, rather than 2? (entered below= I have not entered a Biological Role as either Self or Putative self, as some Figs are showing intermolecular binding, and others intra-mol binding.) Fig 3:- cannot find a cell line, so not entered anywhere. I have not entered a Biological Role as either Self or Putative self, as some Figs are showing intermolecular binding, and others intra-mol binding. Cells were labelled with anti-trastuzumab, anti-pertuzumab (Cy5 -PLA signal detection) and anti-7C2 (AlexaFluor488) antibodies against ERBB2. nagyp@med.unideb.hu 23-Sept-2011: Contacted by JYOTI. orchard Only protein-protein interactions human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Mocanu M.M., Varadi T., Szollosi J., Nagy P. The authors conclude that due to saturation effects, PLA is recommended as a semi-quantitative tool for measuring protein associations. correction - comment from Expt-4:- I am not sure what you want me to do about this:-" Also Fig. 2 only. In all other cases they are using Abs to different epitopes in the same molecule so measuring intramolecular associations not inter." Do you want me to enter another interaction with two interactors and stoich = 0, rather than 2? (entered below= I have not entered a Biological Role as either Self or Putative self, as some Figs are showing intermolecular binding, and others intra-mol binding.) Cells were labelled with anti-trastuzumab, anti-pertuzumab (Cy5 -PLA signal detection) and anti-7C2 (AlexaFluor488) antibodies against ERBB2. nagyp@med.unideb.hu Accepted 2011-AUG-30 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Proteomics (1615-9853) 2011 orchard Only protein-protein interactions imex curation I am not sure what you want me to do about this:-" Also Fig. 2 only. In all other cases they are using Abs to different epitopes in the same molecule so measuring intramolecular associations not inter." Comparative analysis of fluorescence resonance energy transfer (FRET) and proximity ligation assay (PLA). Comparative analysis of fluorescence resonance energy transfer (FRET) and proximity ligation assay (PLA). Proteomics (1615-9853) 2011 imex curation Mocanu M.M., Varadi T., Szollosi J., Nagy P. The authors conclude that due to saturation effects, PLA is recommended as a semi-quantitative tool for measuring protein associations. correction - comment from Expt-4:- I am not sure what you want me to do about this:-" Also Fig. 2 only. In all other cases they are using Abs to different epitopes in the same molecule so measuring intramolecular associations not inter." Do you want me to enter another interaction with two interactors and stoich = 0, rather than 2? (entered below= I have not entered a Biological Role as either Self or Putative self, as some Figs are showing intermolecular binding, and others intra-mol binding.) Fig 3:- cannot find a cell line, so not entered anywhere. I have not entered a Biological Role as either Self or Putative self, as some Figs are showing intermolecular binding, and others intra-mol binding. Cells were labelled with anti-trastuzumab, anti-pertuzumab (Cy5 -PLA signal detection) and anti-7C2 (AlexaFluor488) antibodies against ERBB2. nagyp@med.unideb.hu 23-Sept-2011: Contacted by JYOTI. orchard Only protein-protein interactions human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Mocanu M.M., Varadi T., Szollosi J., Nagy P. The authors conclude that due to saturation effects, PLA is recommended as a semi-quantitative tool for measuring protein associations. correction - comment from Expt-4:- I am not sure what you want me to do about this:-" Also Fig. 2 only. In all other cases they are using Abs to different epitopes in the same molecule so measuring intramolecular associations not inter." Do you want me to enter another interaction with two interactors and stoich = 0, rather than 2? (entered below= I have not entered a Biological Role as either Self or Putative self, as some Figs are showing intermolecular binding, and others intra-mol binding.) Cells were labelled with anti-trastuzumab, anti-pertuzumab (Cy5 -PLA signal detection) and anti-7C2 (AlexaFluor488) antibodies against ERBB2. nagyp@med.unideb.hu Accepted 2011-AUG-30 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Proteomics (1615-9853) 2011 orchard Only protein-protein interactions imex curation Comparative analysis of fluorescence resonance energy transfer (FRET) and proximity ligation assay (PLA). Comparative analysis of fluorescence resonance energy transfer (FRET) and proximity ligation assay (PLA). Proteomics (1615-9853) 2011 imex curation Mocanu M.M., Varadi T., Szollosi J., Nagy P. The authors conclude that due to saturation effects, PLA is recommended as a semi-quantitative tool for measuring protein associations. correction - comment from Expt-4:- I am not sure what you want me to do about this:-" Also Fig. 2 only. In all other cases they are using Abs to different epitopes in the same molecule so measuring intramolecular associations not inter." Do you want me to enter another interaction with two interactors and stoich = 0, rather than 2? (entered below= I have not entered a Biological Role as either Self or Putative self, as some Figs are showing intermolecular binding, and others intra-mol binding.) Fig 3:- cannot find a cell line, so not entered anywhere. I have not entered a Biological Role as either Self or Putative self, as some Figs are showing intermolecular binding, and others intra-mol binding. Cells were labelled with anti-trastuzumab, anti-pertuzumab (Cy5 -PLA signal detection) and anti-7C2 (AlexaFluor488) antibodies against ERBB2. nagyp@med.unideb.hu 23-Sept-2011: Contacted by JYOTI. orchard Only protein-protein interactions human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Mocanu M.M., Varadi T., Szollosi J., Nagy P. The authors conclude that due to saturation effects, PLA is recommended as a semi-quantitative tool for measuring protein associations. correction - comment from Expt-4:- I am not sure what you want me to do about this:-" Also Fig. 2 only. In all other cases they are using Abs to different epitopes in the same molecule so measuring intramolecular associations not inter." Do you want me to enter another interaction with two interactors and stoich = 0, rather than 2? (entered below= I have not entered a Biological Role as either Self or Putative self, as some Figs are showing intermolecular binding, and others intra-mol binding.) Cells were labelled with anti-trastuzumab, anti-pertuzumab (Cy5 -PLA signal detection) and anti-7C2 (AlexaFluor488) antibodies against ERBB2. Cells were labelled with anti-trastuzumab, anti-pertuzumab (Cy5 -PLA signal detection) and anti-7C2 (AlexaFluor488) antibodies against ERBB2. nagyp@med.unideb.hu Accepted 2011-AUG-30 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Proteomics (1615-9853) 2011 orchard Only protein-protein interactions imex curation erbb2_human Receptor tyrosine-protein kinase erbB-2 p185erbB2 MLN19 Proto-oncogene c-ErbB-2 Tyrosine kinase-type cell surface receptor HER2 Proto-oncogene Neu ERBB2 HER2 NEU NGL Metastatic lymph node gene 19 protein protein protein human Homo sapiens Human MELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQSLPTHDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV DIP protein P04626 original sequence version: 198 DIP protein P04626 original sequence version: 122 DIP protein P04626 original sequence version: 206 DIP protein P04626 original sequence version: 195 DIP protein P04626 original sequence version: 150 DIP protein P04626 original sequence version: 179 DIP protein P04626 original sequence version: 146 DIP protein P04626 original sequence version: 147 39E9DFDA04DCF962 egfr_human Epidermal growth factor receptor ERBB1 EGFR Receptor tyrosine-protein kinase erbB-1 Proto-oncogene c-ErbB-1 ERBB HER1 protein protein human Homo sapiens Human MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA DIP protein P00533 original sequence version: 213 DIP protein P00533 original sequence version: 214 DIP protein P00533 original sequence version: 119 DIP protein P00533 original sequence version: 207 DIP protein P00533 original sequence version: 132 DIP protein P00533-1 original sequence version: 197 DIP protein P00533 original sequence version: 163 DIP protein P00533-1 original sequence version: 159 DIP protein P00533 original sequence version: 184 DIP protein P00533-1 original sequence version: 174 DIP protein P00533-1 original sequence version: 191 DIP protein P00533 original sequence version: 218 DIP protein P00533 original sequence version: 221 DIP protein P00533-1 original sequence version: 221 DIP protein P00533 original sequence version: 226 DIP protein P00533 original sequence version: 166 DIP protein P00533-1 original sequence version: 166 DIP protein P00533 original sequence version: 194 DIP protein P00533-1 original sequence version: 194 DIP protein P00533-1 original sequence version: 224 DIP protein P00533 original sequence version: 189 DIP protein P00533-1 original sequence version: 207 DIP protein P00533 original sequence version: 177 DIP protein P00533 original sequence version: 168 D8A2A50B4EFB6ED2 egfr-egfr-5 1 7 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction S1A egfr-egfr-6 2 7 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction S1A EGF her2 3 6 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction Fig 2 her2 4 6 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction S1B, Fig 1, Fig 5, S3. egfr-egfr-7 5 6 unspecified role unspecified role neutral component neutral component colocalization colocalization S2