MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it CESTA, a positive regulator of brassinosteroid biosynthesis. CESTA, a positive regulator of brassinosteroid biosynthesis. EMBO J. (0261-4189) 2011 imex curation Poppenberger B., Rozhon W., Khan M., Husar S., Adam G., Luschnig C., Fujioka S., Sieberer T. brigitte.poppenberger@univie.ac.at Only protein-protein interactions arath-protoplast Arabidopsis thaliana protoplast Protoplast defined as a membrane-bound cell from which the outer wall has been partially or completely removed. fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Poppenberger B., Rozhon W., Khan M., Husar S., Adam G., Luschnig C., Fujioka S., Sieberer T. brigitte.poppenberger@univie.ac.at EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation CESTA, a positive regulator of brassinosteroid biosynthesis. CESTA, a positive regulator of brassinosteroid biosynthesis. EMBO J. (0261-4189) 2011 imex curation Poppenberger B., Rozhon W., Khan M., Husar S., Adam G., Luschnig C., Fujioka S., Sieberer T. brigitte.poppenberger@univie.ac.at Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Poppenberger B., Rozhon W., Khan M., Husar S., Adam G., Luschnig C., Fujioka S., Sieberer T. brigitte.poppenberger@univie.ac.at EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation CESTA, a positive regulator of brassinosteroid biosynthesis. CESTA, a positive regulator of brassinosteroid biosynthesis. EMBO J. (0261-4189) 2011 imex curation Poppenberger B., Rozhon W., Khan M., Husar S., Adam G., Luschnig C., Fujioka S., Sieberer T. brigitte.poppenberger@univie.ac.at Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Poppenberger B., Rozhon W., Khan M., Husar S., Adam G., Luschnig C., Fujioka S., Sieberer T. brigitte.poppenberger@univie.ac.at EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation CESTA, a positive regulator of brassinosteroid biosynthesis. CESTA, a positive regulator of brassinosteroid biosynthesis. EMBO J. (0261-4189) 2011 imex curation Poppenberger B., Rozhon W., Khan M., Husar S., Adam G., Luschnig C., Fujioka S., Sieberer T. brigitte.poppenberger@univie.ac.at Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Poppenberger B., Rozhon W., Khan M., Husar S., Adam G., Luschnig C., Fujioka S., Sieberer T. brigitte.poppenberger@univie.ac.at EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation bh075_arath Transcription factor bHLH75 BHLH75 EN78 Basic helix-loop-helix protein 75 Transcription factor EN 78 bHLH transcription factor bHLH075 F4F7.28 At1g25330 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MARFEPYNYNNGHDPFFAHINQNPELINLDLPASTPSSFMLFSNGALVDANHNNSHFFPNLLHGNTRRKGNKEESGSKRRRKRSEEEEAMNGDETQKPKDVVHVRAKRGQATDSHSLAERVRREKINERLKCLQDLVPGCYKAMGMAVMLDVIIDYVRSLQNQIEFLSMKLSAASACYDLNSLDIEPTDIFQGGNIHSAAEMERILRESVGTQPPNFSSTLPF mint FDB154E92AFC1181 bee3_arath Transcription factor BEE 3 BEE3 BHLH50 EN76 Basic helix-loop-helix protein 50 Protein Brassinosteroid enhanced expression 3 Transcription factor EN 76 bHLH transcription factor bHLH050 F25P22.25 At1g73830 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MANLSSDFQTFTMDDPIRQLAELSNTLHHFQTFPPPFSSSLDSLFFHNQFPDHFPGKSLENNFHQGIFFPSNIQNNEESSSQFDTKKRKSLMEAVSTSENSVSDQTLSTSSAQVSINGNISTKNNSSRRGKRSKNREEEKEREVVHVRARRGQATDSHSIAERVRRGKINERLKCLQDIVPGCYKTMGMATMLDEIINYVQSLQNQVEFLSMKLTAASSYYDFNSETDAVESMQKAKAREAVEMGQGRDGSSVFHSSSWTL mint 61DBE158B7764EF6 bee1_arath Transcription factor BEE 1 BEE1 BHLH44 EN77 Basic helix-loop-helix protein 44 Protein Brassinosteroid enhanced expression 1 Transcription factor EN 77 bHLH transcription factor bHLH044 F15H18.11 F15H18.16 At1g18400 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MANFENLSSDFQTIAMDIYSSITQAADLNNNNSNLHFQTFHPSSTSLESLFLHHHQQQLLHFPGNSPDSSNNFSSTSSFLHSDHNIVDETKKRKALLPTLSSSETSGVSDNTNVIATETGSLRRGKRLKKKKEEEDEKEREVVHVRARRGQATDSHSLAERVRRGKINERLRCLQDMVPGCYKAMGMATMLDEIINYVQSLQNQVEFLSMKLTAASSFYDFNSETDAVDSMQRAKARETVEMGRQTRDGSPVFHLSTWSL 404B546423859BA1 ksg7_arath Shaggy-related protein kinase eta ASK7 BIN2 DWF12 UCU1 ASK-eta Protein BRASSINOSTEROID INSENSITIVE 2 Protein ULTRACURVATA 1 F28A21.120 At4g18710 SK21 Shaggy-related protein kinase 21 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MADDKEMPAAVVDGHDQVTGHIISTTIGGKNGEPKQTISYMAERVVGTGSFGIVFQAKCLETGETVAIKKVLQDRRYKNRELQLMRVMDHPNVVCLKHCFFSTTSKDELFLNLVMEYVPESLYRVLKHYSSANQRMPLVYVKLYMYQIFRGLAYIHNVAGVCHRDLKPQNLLVDPLTHQVKICDFGSAKQLVKGEANISYICSRFYRAPELIFGATEYTTSIDIWSAGCVLAELLLGQPLFPGENAVDQLVEIIKVLGTPTREEIRCMNPHYTDFRFPQIKAHPWHKIFHKRMPPEAIDFASRLLQYSPSLRCTALEACAHPFFDELREPNARLPNGRPFPPLFNFKQEVAGSSPELVNKLIPDHIKRQLGLSFLNQSGT DIP protein Q39011 original sequence version: 100 DIP protein Q39011 original sequence version: 121 DIP protein Q39011 original sequence version: 146 DIP protein Q39011 original sequence version: 129 DIP protein Q39011 original sequence version: 113 86C2FD5F77201168 bhlh75-bee1 2 CES 5 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined BEE1 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association mint SF2D bhlh75-bee3 2 CES 5 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined BEE3 6 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association mint SF2D bhlh75-bhlh75 2 CES 5 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined CES 5 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association mint SF2D bee1-bhlh75 3 CES 5 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell yfp tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined BEE1 7 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell yfp tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association F8C mint bee1-bhlh75-1 1 CES 5 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell cfp tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined BEE1 7 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell yfp tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization F8B mint bee3-bhlh75 1 CES 5 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell cfp tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined BEE3 6 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell yfp tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization SF2C mint ask7-bhlh75 4 CES 5 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra predetermined predetermined participant predetermined enzyme target enzyme target substrate substrate neutral component neutral component purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined BIN2 8 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined phosphorylation phosphorylation reaction phosphorylation F8D mint