MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Red1 promotes the elimination of meiosis-specific mRNAs in vegetatively growing fission yeast. Red1 promotes the elimination of meiosis-specific mRNAs in vegetatively growing fission yeast. EMBO J. (0261-4189) 2011 imex curation Sugiyama T., Sugioka-Sugiyama R. sugiyamt@biol.tsukuba.ac.jp Only protein-protein interactions schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Sugiyama T., Sugioka-Sugiyama R. sugiyamt@biol.tsukuba.ac.jp EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation Red1 promotes the elimination of meiosis-specific mRNAs in vegetatively growing fission yeast. Red1 promotes the elimination of meiosis-specific mRNAs in vegetatively growing fission yeast. EMBO J. (0261-4189) 2011 imex curation Sugiyama T., Sugioka-Sugiyama R. sugiyamt@biol.tsukuba.ac.jp Only protein-protein interactions schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Sugiyama T., Sugioka-Sugiyama R. sugiyamt@biol.tsukuba.ac.jp EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation red1_schpo NURS complex subunit red1 iss3 SPAC1006.03c red1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSRSINLDELRKKALESKKKNEEDESNDSDKEDGEISEDDPVIDQSNSVPPMKVPTFPEQIPQLPPFDRFPGTNANFFPFGAPFMLPPALMFGPNTVPFFPQTASSNKTFSKRKRSSENSFNNRNKAKSSETSDSSNTSQSFKENRALKDTATSRPLALSSDTSYQKSEKAKSEKSPFLSTSKNSDANYSKTTNQKEAEKAVSQLFEVGVRFNDFIAEGIEPSVVHTLFLKLGLDSSSASSQGSLTLSADKAARSAKLRKIDSNLSDTHILPGDNGTPTVLPERKNLISLPLLKQDDWLSSSKPFGSSTPNVVIEFDSDDDGDDFSNSKIEQSNLEKPPSNSENGLTMSRSDYLALLRNKEEEIRRMTKLILRLESNKKPYRSPTSAADMKLPSVPVAAVDNKSKTHLDTFEKVVDLSSKADFVEAGPSISSSGASSSAATTNSDTTEQILEAPWLRKTEQIAVVHEEHPAQIKKSEIDILNNLIEKEEGELTKYQTLVKSKTEILTQLYTRKKQLLEQQGKGNVACLPKESDLSMDSITEVSAQADENSSQILSSKTSNAPNGTTETDFEDKVPLVDYISPFYRFKSYRFNQQFVERVPLKYRSLTYSNKIEPMKVFCKYETTGGVCNDDHCEASHFRDIKMTDDEIIQDLSRYIEGNDEIEKESYKSGLDIVMKNTDENTDFVDVATRIVEYHNLWKSERMTIPVAKVSI 7386E73363CC1AF6 7386E73363CC1AF6 rrp6_schpo Exosome complex exonuclease rrp6 SPAC1F3.01 SPAC3H8.11 rrp6 Ribosomal RNA-processing protein 6 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDESELFKGLMNSTAYCSELAKVDIPFYKSIDTEFNENIKSVSSRFMRLIELLLSKVDRSRAEDIVDVEDIDNRWAEVSDTLDILFEKADYSIDKAQGLLKKPAIETHASTSDVANKKPKKEKLPYKVIHAAHLTKPQLRFRVQPNNSREFVWSWKLTEKPHSLVPLEKIIAQVKLDPSLKNSLPHPYEPEIQNSVYPPWVSEMSNPIDTGSVDETEPIWVSTESQLSDMLKELQNSKEIAVDLEHHDYRSFRGFVCLMQISNREKDWIVDTLELREELEALNVVFTNPNIIKVFHGATMDIIWLQRDFGLYVVNLFDTYYATKVLGFEGHGLAFLLQKYCDYDADKRYQMADWRIRPLPREMLKYAQSDTHYLLYIWDHLRNELISKSAERKENLMQSVFNSSKQISLRKYELEPYDPIYGLGTDGWRNVLTKFGSSKIIGREALMIYRALHDWRDSVARKEDESVRYVLPNRLLIAIAASKPVEAADVFSISKQLTPIARMYVEDIVKVVQEAEKLYNEQVDREKSQFKEVEKQNQPLAVFSESNTLGDYKVDSSVFEISKQNRSKLKTLLANGSAFWIEGQSQDDLRKARKERLFIVNQNIPFSLTLPCTQGHVESELNVKQSTVTEAANPSLNGEKKQEPIVIRDLGLNKQKRDSSKLNHKEPSNPIEERNEDIEPSEASTSVSKKRKQKKKKKNSGKLTIEAEHVSNDSPIINEAPFDYKNQKNFIADLDSDVGKNKFGKRGFNPLNKVSLPKRNTRELKKRKVSDGKSTSY mint DB1FAC172BFB14A2 pab2_schpo Polyadenylate-binding protein 2 pab2 SPBC16E9.12c Poly(A)-binding protein II protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSDQDALDTQEKELLEMKERVAEMEAEAAKLRAMQEQLDNETEALRNDKESIDAQSVYVGNVDYSVTPEELQSHFASCGSVNRVTILCDKFTGHPKGFAYIEFSEPSLVPNALLLNGSMLHERPLKVTPKRTNVPGMSRGRGRGRGRGRGRGRGGYRGRARGFAPY mint A602B5EFC55600E8 mmi1_schpo RNA binding exosome specificity factor Mmi1 mmi1 Meiotic mRNA interception protein 1 YTH domain-containing protein mmi1 SPCC736.12c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSNTNFSTSRSSKSIPELPNLEALRSLWPPPSLNESGDTRSVWTTHTGEPVASSVLSTSGSNNFSSPLKRPAPESHDAPIGRRLMVDDPRLIKHGKYDFSRHCTDYGHSYEWPYFRSLRRESMLYHTSGSYPESQPPYSSYSTDAPHYYHAGSESSAYYDSRSRLHGIQPPPKRRTLSPPPRRLADPVVVGSSRYVEEEVYRRPPYTLASEVPSSASAYQAGYSSYPVRSSPQLSHEDTRHGIASSGSTRYPFVPANTRASHSPSLLEPYAHSLPSSVAPVGAYPEKSSYLLSNSSNDSASRKEKPKARASTPPPLNFSRASEHRNEKGERISMINPRVVLDENGISHRSRYFIMLCDNETAIAHAKKTSIWAVKKDSSKRISDAYKKASVYFIFVAQQTYNALGYAQVVSDLNSTELPFWSDSSHAGGVRIKWIKTCNLFSAEISEIVSHMDHGSEARDGMEMMYDEGSRLCTLINYAIMKRIGRDR mint 329704519DE3AE4A pap_schpo Poly(A) polymerase pla1 pla1 SPBC646.04 Polynucleotide adenylyltransferase protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MTTKQWGITPPISTAPATEQENALNTALINELKNQNLFESPAESEKRVKVLDELQQITTEFVKKVSLAKHMNEKMANEAGGKIFTYGSYRLGVYGPGSDIDTLVVVPKHVSRDNFFQDLEPMLREREEVTDLAAVPDAYVPIIKFKFLGISIDLIFARLSVPRVPRDLELSDNNLLKGVEERCVLSLNGTRVTDQILQLVPNRAVFKHALRAIKFWAQRRAIYANVVGFPGGVAWAMMVARICQLYPNAVSSVIVAKFFRILHQWNWPQPILLKPIEDGPLQVRIWNPKLYPSDKAHRMPIITPAYPSMCATHNITLSTQTIILREMVRAGEIADQIMVKALPWSALFQKHDFFHRYKHYLTITAAAKTAEAQLKWAGLVESKLRHLVTRLELVDAIALAHPFNKGFDKVYNCSSEEEAQQVASGVTLEVAYESTDHEKLANDTVNEEKADNTESKADGSENGEKQIFPVYTTTCYIGLELEKKKGHPIKRLDISWPTQEFYELCKKWDKYDDTLMNVFIKNTKNTALPDEVFEPGEERPKATKKRSTADTAHSTEQLKRQKVSTA mint Polymerase that creates the 3' poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor (CF I) E8E0E4165AAFD3D5 yd14_schpo Uncharacterized protein C4G9.04c SPAC4G9.04c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDLVELDYLSALEDLTFNSKPIIHTLTYIAQENEPYAISIVNAIEKHIQKCPPNCKLPALYLLDSISKNLGAPYTYFFGLHLFSTFMSAYTVVEPRLRLKLDQLLATWKQRPPNSSSLEPVFSPIVTAKIENALLKYKSTILRHQSPLLANTSISSFSAPIDANINSYSSFSDPASSYKPSLPSVPFGFQHISGTSPSPGFITLDSLLSDVNRMIVTEQARFIKNPYDNMAKKRFEILLQLKNVLSSSALPYDQLLAIKNQLAQLEKPASPSTSSVATSAPSVPSALSSISSTPFMKPSIPSTIPTIPSAYSASVSSQPPLTHSYVHPGPQSHKYSLSSGPPASLYNANALTPEESSSIDSLFANLQAAGLVPPSAGGKSQGPQASCTEAVSLTADIDLSKSSLATPRPKLSSLLYENYSNQCANCGRRYGNDPESRKELDKHSDWHFRINKRIRESSLHGINRCWFVMEEEWVNSKEEEDLITETAQEIEEQRQKQMESVRSQYVLTPLDPIAASEPCPICQEKFQSVWHEEAEVWVFMNAVEEEGRIFHATCLQEVRPSENKHSNTNTSTQNLAEAAVSSNIKNATGDASKDSQPDVQNLLQGIDIQSILQALGKRKERDDSMDSMSSKVIKQESK mint 7994539361A29522 mmi1-red1 1 red1 3 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined mmi1 6 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell cfp tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization mint We hypothesized that Red1 co-localizes with Mmi1 and Red1 function is under the control of Mei2-meiRNA in meiosis. To explore this hypothesis, we examined the Red1 localization pattern during meiosis. Unexpectedly, Red1 foci disappeared before the completion of conjugation and re-appeared in spores (Figure 7A). f3a f7a red1-rrp6 1 red1 3 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rrp6 4 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f4a mint pla1-red1 1 red1 3 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pla1 7 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f4a mint pab2-red1 1 red1 3 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pab2 5 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f4a mint red1-yd14 1 red1 3 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pcf11 8 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f4a mint red1-mmi1 2 red1 3 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined mmi1 6 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell cfp tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f3b mint mmi1-red1-1 2 red1 3 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined mmi1 6 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell cfp tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f3b mint pla1-red1-1 2 red1 3 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pla1 7 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association mint In addition, Red1 association with Pla1 and Rrp6 was refractory to RNase treatment (Supplementary Figure S4B), suggesting that Red1 physically binds to Pla1 and Rrp6. f4b sf4b rrp6-red1 2 red1 3 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rrp6 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association mint In addition, Red1 association with Pla1 and Rrp6 was refractory to RNase treatment (Supplementary Figure S4B), suggesting that Red1 physically binds to Pla1 and Rrp6. f4b sf4b mmi1-red1-2 2 red1 3 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined mmi1 6 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell cfp tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association mint sf4a