IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ SHOX interacts with the chondrogenic transcription factors SOX5 and SOX6 to activate the aggrecan enhancer. SHOX interacts with the chondrogenic transcription factors SOX5 and SOX6 to activate the aggrecan enhancer. Hum. Mol. Genet. (0964-6906) 2011 imex curation Aza-Carmona M., Shears DJ., Yuste-Checa P., Barca-Tierno V., Hisado-Oliva A., Belinchon A., Benito-Sanz S., Rodriguez JI., Argente J., Campos-Barros A., Scambler PJ., Heath KE. colocalisation studies not included since i could only see colocalisation to the cells in question not to any specific region (all the cells had staining.) kheath.hulp@salud.madrid.org 9.5-10.5 dpc cDNA library orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Aza-Carmona M., Shears DJ., Yuste-Checa P., Barca-Tierno V., Hisado-Oliva A., Belinchon A., Benito-Sanz S., Rodriguez JI., Argente J., Campos-Barros A., Scambler PJ., Heath KE. colocalisation studies not included since i could only see colocalisation to the cells in question not to any specific region (all the cells had staining.) kheath.hulp@salud.madrid.org Accepted 2011-FEB-03 by ORCHARD 9.5-10.5 dpc cDNA library Hum. Mol. Genet. (0964-6906) 2011 orchard Only protein-protein interactions imex curation SHOX interacts with the chondrogenic transcription factors SOX5 and SOX6 to activate the aggrecan enhancer. SHOX interacts with the chondrogenic transcription factors SOX5 and SOX6 to activate the aggrecan enhancer. Hum. Mol. Genet. (0964-6906) 2011 imex curation Aza-Carmona M., Shears DJ., Yuste-Checa P., Barca-Tierno V., Hisado-Oliva A., Belinchon A., Benito-Sanz S., Rodriguez JI., Argente J., Campos-Barros A., Scambler PJ., Heath KE. colocalisation studies not included since i could only see colocalisation to the cells in question not to any specific region (all the cells had staining.) kheath.hulp@salud.madrid.org 9.5-10.5 dpc cDNA library orchard Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Aza-Carmona M., Shears DJ., Yuste-Checa P., Barca-Tierno V., Hisado-Oliva A., Belinchon A., Benito-Sanz S., Rodriguez JI., Argente J., Campos-Barros A., Scambler PJ., Heath KE. colocalisation studies not included since i could only see colocalisation to the cells in question not to any specific region (all the cells had staining.) kheath.hulp@salud.madrid.org Accepted 2011-FEB-03 by ORCHARD 9.5-10.5 dpc cDNA library Hum. Mol. Genet. (0964-6906) 2011 orchard Only protein-protein interactions imex curation SHOX interacts with the chondrogenic transcription factors SOX5 and SOX6 to activate the aggrecan enhancer. SHOX interacts with the chondrogenic transcription factors SOX5 and SOX6 to activate the aggrecan enhancer. Hum. Mol. Genet. (0964-6906) 2011 imex curation Aza-Carmona M., Shears DJ., Yuste-Checa P., Barca-Tierno V., Hisado-Oliva A., Belinchon A., Benito-Sanz S., Rodriguez JI., Argente J., Campos-Barros A., Scambler PJ., Heath KE. colocalisation studies not included since i could only see colocalisation to the cells in question not to any specific region (all the cells had staining.) kheath.hulp@salud.madrid.org 9.5-10.5 dpc cDNA library orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Aza-Carmona M., Shears DJ., Yuste-Checa P., Barca-Tierno V., Hisado-Oliva A., Belinchon A., Benito-Sanz S., Rodriguez JI., Argente J., Campos-Barros A., Scambler PJ., Heath KE. colocalisation studies not included since i could only see colocalisation to the cells in question not to any specific region (all the cells had staining.) kheath.hulp@salud.madrid.org Accepted 2011-FEB-03 by ORCHARD 9.5-10.5 dpc cDNA library Hum. Mol. Genet. (0964-6906) 2011 orchard Only protein-protein interactions imex curation sox6_mouse Transcription factor SOX-6 Sox6 Sox-6 SOX-LZ protein protein mouse Mus musculus Mouse MSSKQATSPFACTADGEEAMTQDLTSREKEEGSDQHPASHLPLHPIMHNKPHSEELPTLVSTIQQDADWDSVLSSQQRMESENNKLCSLYSFRNTSTSPHKPDEGSREREIMNSVTFGTPERRKGSLADVVDTLKQKKLEEMTRTEQEDSSCMEKLLSKDWKEKMERLNTSELLGEIKGTPESLAEKERQLSTMITQLISLREQLLAAHDEQKKLAASQIEKQRQQMDLARQQQEQIARQQQQLLQQQHKINLLQQQIQVQGHMPPLMIPIFPHDQRTLAAAAAAQQGFLFPPGITYKPGDNYPVQFIPSTMAAAAASGLSPLQLQKGHVSHPQINPRLKGISDRFGRNLDPSEHGGGHSYNHRQIEQLYAAQLASMQVSPGAKMPSTPQPPNSAGAVSPTGIKNEKRGTSPVTQVKDETTAQPLNLSSRPKTAEPVKSPTSPTQNLFPASKTSPVNLPNKSSIPSPIGGSLGRGSSLDILSSLNSPALFGDQDTVMKAIQEARKMREQIQREQQQQPHGVDGKLSSMNNMGLSNCRTEKERTRFENLGPQLTGKSSEDGKLGPGVIDLTRPEDAEGSKAMNGSAAKLQQYYCWPTGGATVAEARVYRDARGRASSEPHIKRPMNAFMVWAKDERRKILQAFPDMHNSNISKILGSRWKSMSNQEKQPYYEEQARLSKIHLEKYPNYKYKPRPKRTCIVDGKKLRIGEYKQLMRSRRQEMRQFFTVGQQPQMPITTGTGVVYPGAITMATTTPSPQMTSDCSSTSASPEPSLPVIQSTYGMKMDGASLAGNDMINGEDEMEAYDDYEDDPKSDYSSENEAPEPVSAN F777BAB7CFB1E93B sox5_human Transcription factor SOX-5 SOX5 protein protein human Homo sapiens Human MLTDPDLPQEFERMSSKRPASPYGEADGEVAMVTSRQKVEEEESDGLPAFHLPLHVSFPNKPHSEEFQPVSLLTQETCGHRTPTSQHNTMEVDGNKVMSSFAPHNSSTSPQKAEEGGRQSGESLSSTALGTPERRKGSLADVVDTLKQRKMEELIKNEPEETPSIEKLLSKDWKDKLLAMGSGNFGEIKGTPESLAEKERQLMGMINQLTSLREQLLAAHDEQKKLAASQIEKQRQQMELAKQQQEQIARQQQQLLQQQHKINLLQQQIQVQGQLPPLMIPVFPPDQRTLAAAAQQGFLLPPGFSYKAGCSDPYPVQLIPTTMAAAAAATPGLGPLQLQQLYAAQLAAMQVSPGGKLPGIPQGNLGAAVSPTSIHTDKSTNSPPPKSKDEVAQPLNLSAKPKTSDGKSPTSPTSPHMPALRINSGAGPLKASVPAALASPSARVSTIGYLNDHDAVTKAIQEARQMKEQLRREQQVLDGKVAVVNSLGLNNCRTEKEKTTLESLTQQLAVKQNEEGKFSHAMMDFNLSGDSDGSAGVSESRIYRESRGRGSNEPHIKRPMNAFMVWAKDERRKILQAFPDMHNSNISKILGSRWKAMTNLEKQPYYEEQARLSKQHLEKYPDYKYKPRPKRTCLVDGKKLRIGEYKAIMRNRRQEMRQYFNVGQQAQIPIATAGVVYPGAIAMAGMPSPHLPSEHSSVSSSPEPGMPVIQSTYGVKGEEPHIKEEIQAEDINGEIYDEYDEEEDDPDVDYGSDSENHIAGQAN 02B5B13429A59CAA shox_human Short stature homeobox protein SHOX PHOG Pseudoautosomal homeobox-containing osteogenic protein Short stature homeobox-containing protein protein protein human Homo sapiens Human MEELTAFVSKSFDQKSKDGNGGGGGGGGKKDSITYREVLESGLARSRELGTSDSSLQDITEGGGHCPVHLFKDHVDNDKEKLKEFGTARVAEGIYECKEKREDVKSEDEDGQTKLKQRRSRTNFTLEQLNELERLFDETHYPDAFMREELSQRLGLSEARVQVWFQNRRAKCRKQENQMHKGVILGTANHLDACRVAPYVNMGALRMPFQQVQAQLQLEGVAHAHPHLHPHLAAHAPYLMFPPPPFGLPIASLAESASAAAVVAAAAKSNSKNSSIADLRLKARKHAEALGL 0F2A61A3051CB360 sox5_mouse Transcription factor SOX-5 Sox5 Sox-5 protein protein mouse Mus musculus Mouse MLTDPDLPQEFERMSSKRPASPYGETDGEVAMVTSRQKVEEEESERLPAFHLPLHVSFPNKPHSEEFQPVSLLTQETCGPRTPTVQHNTMEVDGNKVMSSLSPYNSSTSPQKAEEGGRQSGESVSSAALGTPERRKGSLADVVDTLKQRKMEELIKNEPEDTPSIEKLLSKDWKDKLLAMGSGNFGEIKGTPESLAEKERQLMGMINQLTSLREQLLAAHDEQKKLAASQIEKQRQQMELAKQQQEQIARQQQQLLQQQHKINLLQQQIQVQGQLPPLMIPVFPPDQRTLAAAAQQGFLLPPGFSYKAGCSDPYPVQLIPTTMAAAAAATPGLGPLQLQQFYAAQLAAMQVSPGGKLLGLPQGNLGAAVSPTSIHTDKSTNSPPPKSKDEVAQPLNLSAKPKTSDGKSPASPTSPHMPALRINSGAGPLKASVPAALASPSARVSTIGYLNDHDAVTKAIQEARQMKEQLRREQQALDGKVAVVNSIGLSNCRTEKEKTTLESLTQQLAVKQNEEGKFSHGMMDFNMSGDSDGSAGVSESRIYRESRGRGSNEPHIKRPMNAFMVWAKDERRKILQAFPDMHNSNISKILGSRWKAMTNLEKQPYYEEQARLSKQHLEKYPDYKYKPRPKRTCLVDGKKLRIGEYKAIMRNRRQEMRQYFNVGQQAQIPIATAGVVYPSAIAMAGMPSPHLPSEHSSVSSSPEPGMPVIQSTYGAKGEEPHIKEEIQAEDINGEIYEEYDEEEEDPDVDYGSDSENHIAGQAN E38B1470ECCB1DE7 sox6_human Transcription factor SOX-6 SOX6 protein protein human Homo sapiens Human MSSKQATSPFACAADGEDAMTQDLTSREKEEGSDQHVASHLPLHPIMHNKPHSEELPTLVSTIQQDADWDSVLSSQQRMESENNKLCSLYSFRNTSTSPHKPDEGSRDREIMTSVTFGTPERRKGSLADVVDTLKQKKLEEMTRTEQEDSSCMEKLLSKDWKEKMERLNTSELLGEIKGTPESLAEKERQLSTMITQLISLREQLLAAHDEQKKLAASQIEKQRQQMDLARQQQEQIARQQQQLLQQQHKINLLQQQIQVQGHMPPLMIPIFPHDQRTLAAAAAAQQGFLFPPGITYKPGDNYPVQFIPSTMAAAAASGLSPLQLQKGHVSHPQINQRLKGLSDRFGRNLDTFEHGGGHSYNHKQIEQLYAAQLASMQVSPGAKMPSTPQPPNTAGTVSPTGIKNEKRGTSPVTQVKDEAAAQPLNLSSRPKTAEPVKSPTSPTQNLFPASKTSPVNLPNKSSIPSPIGGSLGRGSSLDILSSLNSPALFGDQDTVMKAIQEARKMREQIQREQQQQQPHGVDGKLSSINNMGLNSCRNEKERTRFENLGPQLTGKSNEDGKLGPGVIDLTRPEDAEGSKAMNGSAAKLQQYYCWPTGGATVAEARVYRDARGRASSEPHIKRPMNAFMVWAKDERRKILQAFPDMHNSNISKILGSRWKSMSNQEKQPYYEEQARLSKIHLEKYPNYKYKPRPKRTCIVDGKKLRIGEYKQLMRSRRQEMRQFFTVGQQPQIPITTGTGVVYPGAITMATTTPSPQMTSDCSSTSASPEPSLPVIQSTYGMKTDGGSLAGNEMINGEDEMEMYDDYEDDPKSDYSSENEAPEAVSAN 38CA781528C839CF shox-sox5 1 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association shox-sox6-1 1 6 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association shox-sox5 3 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 1 sox6-shox 3 6 unspecified role unspecified role prey prey O15266:p.Leu132Val mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L V Wed May 02 17:08:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu132val' Tue Jun 30 17:24:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu132Val' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O15266:p.Lys116Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K E Wed May 02 16:19:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys116glu' Tue Jun 30 19:28:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys116Glu' O15266:p.Arg168Tyr mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R Y Wed May 02 19:55:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg168tyr' Tue Jun 30 20:13:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg168Tyr' O15266:p.Arg173Cys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R C Wed May 02 18:52:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg173cys' Tue Jun 30 17:40:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg173Cys' O15266:p.Ala170Pro mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain A P Wed May 02 15:42:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala170pro' Tue Jun 30 19:49:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala170Pro' O15266:p.Arg195Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R P Wed May 02 15:14:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg195pro' Tue Jun 30 18:45:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg195Pro' O15266:p.Ala233Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain A E Wed May 02 16:31:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala233glu' Tue Jun 30 19:02:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala233Glu' O15266:p.Asp278Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain D A Wed May 02 20:12:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp278ala' Tue Jun 30 19:44:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp278Ala' 8 unspecified role unspecified role bait bait physical association physical association 1, 4, 5 shox-shox 3 6 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 1 shox-sox5-1 2 6 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2 shox-sox6-2 2 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2 sox6-shox-1 3 8 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 2nd region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey physical association physical association 1, 6 Binding of deletion mutant 263-787 increased binding over full-length protein.