MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Interaction of calmodulin with Sec61α limits Ca2+ leakage from the endoplasmic reticulum. Interaction of calmodulin with Sec61α limits Ca2+ leakage from the endoplasmic reticulum. EMBO J. (0261-4189) 2011 imex curation Erdmann F., Schäuble N., Lang S., Jung M., Honigmann A., Ahmad M., Dudek J., Benedix J., Harsman A., Kopp A., Helms V., Cavalié A., Wagner R., Zimmermann R. Only protein-protein interactions in vitro In vitro density sedimentation cosedimentation through density gradient density sedimentation predetermined predetermined participant predetermined Erdmann F., Schäuble N., Lang S., Jung M., Honigmann A., Ahmad M., Dudek J., Benedix J., Harsman A., Kopp A., Helms V., Cavalié A., Wagner R., Zimmermann R. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation Interaction of calmodulin with Sec61α limits Ca2+ leakage from the endoplasmic reticulum. Interaction of calmodulin with Sec61α limits Ca2+ leakage from the endoplasmic reticulum. EMBO J. (0261-4189) 2011 imex curation Erdmann F., Schäuble N., Lang S., Jung M., Honigmann A., Ahmad M., Dudek J., Benedix J., Harsman A., Kopp A., Helms V., Cavalié A., Wagner R., Zimmermann R. Only protein-protein interactions in vitro In vitro peptide array peptide array predetermined predetermined participant predetermined Erdmann F., Schäuble N., Lang S., Jung M., Honigmann A., Ahmad M., Dudek J., Benedix J., Harsman A., Kopp A., Helms V., Cavalié A., Wagner R., Zimmermann R. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation Interaction of calmodulin with Sec61α limits Ca2+ leakage from the endoplasmic reticulum. Interaction of calmodulin with Sec61α limits Ca2+ leakage from the endoplasmic reticulum. EMBO J. (0261-4189) 2011 imex curation Erdmann F., Schäuble N., Lang S., Jung M., Honigmann A., Ahmad M., Dudek J., Benedix J., Harsman A., Kopp A., Helms V., Cavalié A., Wagner R., Zimmermann R. Only protein-protein interactions in vitro In vitro fcs fluorescence correlation spectroscopy FCS FCS fcs predetermined predetermined participant predetermined Erdmann F., Schäuble N., Lang S., Jung M., Honigmann A., Ahmad M., Dudek J., Benedix J., Harsman A., Kopp A., Helms V., Cavalié A., Wagner R., Zimmermann R. EMBO J. (0261-4189) 2011 Only protein-protein interactions imex curation calm_rat Calmodulin Calm1 Calm Cam Cam1 Calm2 Cam2 Camb Calm3 Cam3 Camc CaMII protein protein rat Rattus norvegicus (Rat) Rat MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK DIP protein P62161 original sequence version: 125 DIP protein P62161 original sequence version: 51 DIP protein P62161 original sequence version: 55 DIP protein P62161 original sequence version: 59 DIP protein P62161 original sequence version: 80 DIP protein P62161 original sequence version: 81 DIP protein P62161 original sequence version: 132 DIP protein P62161 original sequence version: 96 6B4BC3FCDE10727B s61a1_canlf Protein transport protein Sec61 subunit alpha isoform 1 SEC61A1 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MAIKFLEVIKPFCVILPEIQKPERKIQFKEKVLWTAITLFIFLVCCQIPLFGIMSSDSADPFYWMRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVGDTPKDRALFNGAQKLFGMIITIGQSIVYVMTGMYGDPSEMGAGICLLITIQLFVAGLIVLLLDELLQKGYGLGSGISLFIATNICETIVWKAFSPTTVNTGRGMEFEGAIIALFHLLATRTDKVRALREAFYRQNLPNLMNLIATIFVFAVVIYFQGFRVDLPIKSARYRGQYNTYPIKLFYTSNIPIILQSALVSNLYVISQMLSARFSGNLLVSLLGTWSDTSSGGPARAYPVGGLCHYLSPPESFGSVLEDPVHAVVYIVFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRETSMVHELNRYIPTAAAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQYFEIFVKEQSEVGSMGALLF mint Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins B1A9B7A6697327FE sc61b_canlf Protein transport protein Sec61 subunit beta SEC61B protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGRTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS mint Necessary for protein translocation in the endoplasmic reticulum 5FAFF4197A62FA49 calm1-sec61a1 3 Sec61 alpha 5 peptide synthesis peptide synthesis predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Calmodulin 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction f1d mint We found that CaM binding to the IQ488 peptide in the presence of saturating calcium concentrations (4 mM) occurred via a single-site binding mechanism (Hill slope: 1.1±0.19; KD 121±28 nM; Figure 1D) and GST-CaM bound to the IQ488 peptide with a Hill slope of 0.88±0.07 and a KD of 115±12 nM (Figure 1E). calm1-sec61a1-1 3 Sec61 alpha 5 peptide synthesis peptide synthesis predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Calmodulin 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction f1e mint We found that CaM binding to the IQ488 peptide in the presence of saturating calcium concentrations (4 mM) occurred via a single-site binding mechanism (Hill slope: 1.1±0.19; KD 121±28 nM; Figure 1D) and GST-CaM bound to the IQ488 peptide with a Hill slope of 0.88±0.07 and a KD of 115±12 nM (Figure 1E). sec61a1-calm1 2 Sec61 alpha 5 peptide synthesis peptide synthesis predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified P38377:p.Arg24Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Tue Jul 12 10:58:03 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 18:40:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg24ala' Tue Jun 30 20:11:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg24Ala' binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Calmodulin 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction f1f mint Consistent with the FCS results, the wild-type Sec61α IQ motif bound 14C-labelled GST-CaM in the presence of free calcium (Figure 1F, spots 1, 7, and 10; defined as 100% binding). When Ca2+ was chelated with EGTA, GST-CaM binding was nearly abolished (spots 1, 7, and 10; 17±5% binding). The exchange of the conserved residues to alanine at positions 7 and 11 drastically reduced CaM binding (spots 5 and 6; 24±4.8% binding). sec61a1-calm1-1 2 Sec61 alpha 5 peptide synthesis peptide synthesis predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P38377:p.Lys29Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A Tue Jul 12 10:58:00 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 15:06:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys29ala' Tue Jun 30 16:55:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys29Ala' Calmodulin 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction f1f mint Consistent with the FCS results, the wild-type Sec61α IQ motif bound 14C-labelled GST-CaM in the presence of free calcium (Figure 1F, spots 1, 7, and 10; defined as 100% binding). When Ca2+ was chelated with EGTA, GST-CaM binding was nearly abolished (spots 1, 7, and 10; 17±5% binding). The exchange of the conserved residues to alanine at positions 7 and 11 drastically reduced CaM binding (spots 5 and 6; 24±4.8% binding). calm1-sec61b 1 Sec61 beta 6 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level Calmodulin 4 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization mint f2b f2c f2d f2e