UniProt UniProt http://www.uniprot.org The cytoplasmic adaptor protein Dok7 activates the receptor tyrosine kinase MuSK via dimerization. The cytoplasmic adaptor protein Dok7 activates the receptor tyrosine kinase MuSK via dimerization. Mol. Cell (1097-2765) 2010 mimix curation Bergamin E., Hallock PT., Burden SJ., Hubbard SR. stevan.hubbard@med.nyu.edu 23-Sept-2011: Contacted by JYOTI. orchard in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Bergamin E., Hallock PT., Burden SJ., Hubbard SR. stevan.hubbard@med.nyu.edu Accepted 2011-AUG-04 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Mol. Cell (1097-2765) 2010 orchard mimix curation The cytoplasmic adaptor protein Dok7 activates the receptor tyrosine kinase MuSK via dimerization. The cytoplasmic adaptor protein Dok7 activates the receptor tyrosine kinase MuSK via dimerization. Mol. Cell (1097-2765) 2010 mimix curation Bergamin E., Hallock PT., Burden SJ., Hubbard SR. stevan.hubbard@med.nyu.edu 23-Sept-2011: Contacted by JYOTI. orchard in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Bergamin E., Hallock PT., Burden SJ., Hubbard SR. stevan.hubbard@med.nyu.edu Accepted 2011-AUG-04 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Mol. Cell (1097-2765) 2010 orchard mimix curation The cytoplasmic adaptor protein Dok7 activates the receptor tyrosine kinase MuSK via dimerization. The cytoplasmic adaptor protein Dok7 activates the receptor tyrosine kinase MuSK via dimerization. Mol. Cell (1097-2765) 2010 mimix curation Bergamin E., Hallock PT., Burden SJ., Hubbard SR. stevan.hubbard@med.nyu.edu 23-Sept-2011: Contacted by JYOTI. orchard in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Bergamin E., Hallock PT., Burden SJ., Hubbard SR. stevan.hubbard@med.nyu.edu Accepted 2011-AUG-04 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Mol. Cell (1097-2765) 2010 orchard mimix curation The cytoplasmic adaptor protein Dok7 activates the receptor tyrosine kinase MuSK via dimerization. The cytoplasmic adaptor protein Dok7 activates the receptor tyrosine kinase MuSK via dimerization. Mol. Cell (1097-2765) 2010 mimix curation Bergamin E., Hallock PT., Burden SJ., Hubbard SR. stevan.hubbard@med.nyu.edu 23-Sept-2011: Contacted by JYOTI. orchard in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Bergamin E., Hallock PT., Burden SJ., Hubbard SR. stevan.hubbard@med.nyu.edu Accepted 2011-AUG-04 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Mol. Cell (1097-2765) 2010 orchard mimix curation dok7_mouse Protein Dok-7 Dok7 Downstream of tyrosine kinase 7 protein protein mouse Mus musculus Mouse MTEAALVEGQVKLRDGKKWKSRWLVLRKPSPVADCLLMLVYKDKCERSKGLRERSSLTLEDICGLEPALPYEGLAHTLAIICLSQAVMLGFDSHEAMCAWDTRIRYALGEVHRFHVTVAPGTKLESGPATLHLCNDILVLARDIPPTVMGQWKLSDLRRYGAVPNGFIFEGGTRCGYWAGVFFLSSAEGEQMSFLFDCIVRGISPTKGPFGLRPVLPDPSSGGPSASEERVAQEALEALQLEKRLSLLSHSGRPGSGGDDRSLSSSSSEASHSDISASSRLTAWPEQSSSSAGTSQEGPGLVAAQGPGEAMLGASRPPLKPLRPRQLQEVGRQSSSDSGIATGSHSSYSGSFSSYAGSNLDVWRAGEEFGSLLSLPPGASAPEPRLCACPPGAAEYQVPTSLRHHYDTPRSLRQAPRDPSPASQGSSDHGSATDLGGQAPTGCPSSWLGARRRGQATEGPGSDAALPSPSPGESWEAGSPHAGPPPAFFLSCSICGGLKVKPPP musk_mouse Muscle, skeletal receptor tyrosine-protein kinase Musk Nsk2 Muscle-specific tyrosine-protein kinase receptor protein protein mouse Mus musculus Mouse MRELVNIPLLQMLTLVAFSGTEKLPKAPVITTPLETVDALVEEVATFMCAVESYPQPEISWTRNKILIKLFDTRYSIRENGQLLTILSVEDSDDGIYCCIANNGVGGAVESCGALQVKMKPKITRPPINVKIIEGLKAVLPCTTMGNPKPSVSWIKGDNALRENSRIAVLESGSLRIHNVQKEDAGQYRCVAKNSLGTAYSKLVKLEVEVFARILRAPESHNVTFGSFVTLRCTAIGIPVPTISWIENGNAVSSGSIQESVKDRVIDSRLQLFITKPGLYTCIATNKHGEKFSTAKAAATVSIAEWSKSQKDSQGYCAQYRGEVCDAVLAKDALVFFNTSYRDPEDAQELLIHTAWNELKAVSPLCRPAAEALLCNHLFQECSPGVVPTPMPICREYCLAVKELFCAKEWQAMEGKAHRGLYRSGMHLLPVPECSKLPSMHRDPTACTRLPYLDYKKENITTFPSITSSRPSADIPNLPASTSSFAVSPAYSMTVIISIVSSFALFALLTIATLYCCRRRKEWKNKKRESTAVTLTTLPSELLLDRLHPNPMYQRMPLLLNPKLLSLEYPRNNIEYVRDIGEGAFGRVFQARAPGLLPYEPFTMVAVKMLKEEASADMQADFQREAALMAEFDNPNIVKLLGVCAVGKPMCLLFEYMAYGDLNEFLRSMSPHTVCSLSHSDLSTRARVSSPGPPPLSCAEQLCIARQVAAGMAYLSERKFVHRDLATRNCLVGETMVVKIADFGLSRNIYSADYYKADGNDAIPIRWMPPESIFYNRYTTESDVWAYGVVLWEIFSYGLQPYYGMAHEEVIYYVRDGNILACPENCPLELYNLMRLCWSKLPADRPSFCSIHRILQRMCERAEGTVGV musk_rat Muscle, skeletal receptor tyrosine protein kinase Musk Muscle-specific tyrosine protein kinase receptor protein protein rat Rattus norvegicus (Rat) Rat MRELVNIPLLQMLTLVAFSGTEKLPKAPVITTPLETVDALVEEVATFMCAVESYPQPEISWTRNKILIKLFDTRYSIRENGQLLTILSVEDSDDGIYCCTANNGVGGAVESCGALQVKMKPKITRPPINVKIIEGLKAVLPCTTMGNPKPSVSWIKGDSALRENSRIAVLESGSLRIHNVQKEDAGQYRCVAKNSLGTAYSKLVKLEVEVFARILRAPESHNVTFGSFVTLRCTAIGMPVPTISWIENGNAVSSGSIQENVKDRVIDSRLQLFITKPGLYTCIATNKHGEKFSTAKAAATVSIAEWSKSQKESKGYCAQYRGEVCDAVLVKDSLVFFNTSYPDPEEAQELLIHTAWNELKAVSPLCRPAAEALLCNHLFQECSPGVLPTPMPICREYCLAVKELFCAKEWLAMEGKTHRGLYRSGMHFLPVPECSKLPSMHQDPTACTRLPYLDYKKENITTFPSITSSKPSVDIPNLPASTSSFAVSPAYSMTVIISIMSCFAVFALLTITTLYCCRRRREWKNKKRESAAVTLTTLPSELLLDRLHPNPMYQRMPLLLNPKLLSLEYPRNNIEYVRDIGEGAFGRVFQARAPGLLPYEPFTMVAVKMLKEEASADMQADFQREAALMAEFDNPNIVKLLGVCAVGKPMCLLFEYMAYGDLNEFLRSMSPHTVCSLSHSDLSTRARVSSPGPPPLSCAEQLCIARQVAAGMAYLSERKFVHRDLATRNCLVGENMVVKIADFGLSRNIYSADYYKADGNDAIPIRWMPPESIFYNRYTTESDVWAYGVVLWEIFSYGLQPYYGMAHEEVIYYVRDGNILACPENCPLELYNLMRLCWSKLPADRPSFCSIHRILQRMCERAEGTVGV C146B4E74EE25B24 dok7-musk 4 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 2.6 A R factor 0.255 R free 0.302 for the synthetic peptide, residue numbering fits mouse dok7-dok7 4 5 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction 2.6 A monomer in solution, dimer (tetramer?) in crystal. Authors speculate the protein may dimerize upon MUSK binding. See also text p.103 and Fig 3 This crystal was described in the article but not deposited with PDB, as only partially refined. dok7-musk-2 3 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component cytoplasmic region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 20 18 direct interaction direct interaction Fig 3A dok7-musk-3 2 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 23 25 direct interaction direct interaction supplementary fig S1 for the synthetic peptide, residue numbering fits mouse musk-musk-2 1 6 putative self putative self putative self putative self phosphorylation phosphorylation reaction phosphorylation true fig 3