UniProt UniProt http://www.uniprot.org A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. Nat. Cell Biol. (1465-7392) 2010 mimix curation Srikanth S., Jung HJ., Kim KD., Souda P., Whitelegge J., Gwack Y. ygwack@mednet.ucla.edu 26-Sep-2011: Contacted by JYOTI. ORCHARD human Homo sapiens Human fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Srikanth S., Jung HJ., Kim KD., Souda P., Whitelegge J., Gwack Y. ygwack@mednet.ucla.edu Accepted 2011-JUL-20 by ORCHARD 26-Sep-2011: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2010 ORCHARD mimix curation A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. Nat. Cell Biol. (1465-7392) 2010 mimix curation Srikanth S., Jung HJ., Kim KD., Souda P., Whitelegge J., Gwack Y. ygwack@mednet.ucla.edu 26-Sep-2011: Contacted by JYOTI. ORCHARD human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Srikanth S., Jung HJ., Kim KD., Souda P., Whitelegge J., Gwack Y. ygwack@mednet.ucla.edu Accepted 2011-JUL-20 by ORCHARD 26-Sep-2011: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2010 ORCHARD mimix curation A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. Nat. Cell Biol. (1465-7392) 2010 mimix curation Srikanth S., Jung HJ., Kim KD., Souda P., Whitelegge J., Gwack Y. ygwack@mednet.ucla.edu 26-Sep-2011: Contacted by JYOTI. ORCHARD human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Srikanth S., Jung HJ., Kim KD., Souda P., Whitelegge J., Gwack Y. ygwack@mednet.ucla.edu Accepted 2011-JUL-20 by ORCHARD 26-Sep-2011: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2010 ORCHARD mimix curation A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. Nat. Cell Biol. (1465-7392) 2010 mimix curation Srikanth S., Jung HJ., Kim KD., Souda P., Whitelegge J., Gwack Y. ygwack@mednet.ucla.edu 26-Sep-2011: Contacted by JYOTI. ORCHARD human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Srikanth S., Jung HJ., Kim KD., Souda P., Whitelegge J., Gwack Y. ygwack@mednet.ucla.edu Accepted 2011-JUL-20 by ORCHARD 26-Sep-2011: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2010 ORCHARD mimix curation A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. Nat. Cell Biol. (1465-7392) 2010 mimix curation Srikanth S., Jung HJ., Kim KD., Souda P., Whitelegge J., Gwack Y. ygwack@mednet.ucla.edu 26-Sep-2011: Contacted by JYOTI. ORCHARD in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Srikanth S., Jung HJ., Kim KD., Souda P., Whitelegge J., Gwack Y. ygwack@mednet.ucla.edu Accepted 2011-JUL-20 by ORCHARD 26-Sep-2011: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2010 ORCHARD mimix curation A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. Nat. Cell Biol. (1465-7392) 2010 mimix curation Srikanth S., Jung HJ., Kim KD., Souda P., Whitelegge J., Gwack Y. ygwack@mednet.ucla.edu 26-Sep-2011: Contacted by JYOTI. ORCHARD in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Srikanth S., Jung HJ., Kim KD., Souda P., Whitelegge J., Gwack Y. ygwack@mednet.ucla.edu Accepted 2011-JUL-20 by ORCHARD 26-Sep-2011: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2010 ORCHARD mimix curation crcm1_human Calcium release-activated calcium channel protein 1 ORAI1 CRACM1 TMEM142A Protein orai-1 Transmembrane protein 142A protein protein human Homo sapiens Human MHPEPAPPPSRSSPELPPSGGSTTSGSRRSRRRSGDGEPPGAPPPPPSAVTYPDWIGQSYSEVMSLNEHSMQALSWRKLYLSRAKLKASSRTSALLSGFAMVAMVEVQLDADHDYPPGLLIAFSACTTVLVAVHLFALMISTCILPNIEAVSNVHNLNSVKESPHERMHRHIELAWAFSTVIGTLLFLAEVVLLCWVKFLPLKKQPGQPRPTSKPPASGAAANVSTSGITPGQAAAIASTTIMVPFGLIFIVFAVHFYRSLVSHKTDRQFQELNELAEFARLQDQLDHRGDHPLTPGSHYA DIP protein Q96D31 original sequence version: 95 DIP protein Q96D31 original sequence version: 38 DIP protein Q96D31 original sequence version: 58 DIP protein Q96D31 original sequence version: 55 DIP protein Q96D31 original sequence version: 57 DIP protein Q96D31 original sequence version: 53 DIP protein Q96D31 original sequence version: 63 DIP protein Q96D31 original sequence version: 76 DIP protein Q96D31 original sequence version: 79 DIP protein Q96D31 original sequence version: 56 F85F7C06F36628D4 stim1_human Stromal interaction molecule 1 STIM1 GOK protein protein human Homo sapiens Human MDVCVRLALWLLWGLLLHQGQSLSHSHSEKATGTSSGANSEESTAAEFCRIDKPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVVQWLITYVELPQYEETFRKLQLSGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAEKQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGFQIVNNPGIHSLVAALNIDPSWMGSTRPNPAHFIMTDDVDDMDEEIVSPLSMQSPSLQSSVRQRLTEPQHGLGSQRDLTHSDSESSLHMSDRQRVAPKPPQMSRAADEALNAMTSNGSHRLIEGVHPGSLVEKLPDSPALAKKALLALNHGLDKAHSLMELSPSAPPGGSPHLDSSRSHSPSSPDPDTPSPVGDSRALQASRNTRIPHLAGKKAVAEEDNGSIGEETDSSPGRKKFPLKIFKKPLKK DIP protein Q13586 original sequence version: 141 DIP protein Q13586 original sequence version: 99 DIP protein Q13586 original sequence version: 108 DIP protein Q13586 original sequence version: 121 DIP protein Q13586 original sequence version: 125 DIP protein Q13586 original sequence version: 100 FFB5E7CB3D68A7A6 efc4b_human EF-hand calcium-binding domain-containing protein 4B CRACR2A Calcium release-activated calcium channel regulator 2A Calcium release-activated channel regulator 2A EFCAB4B RAB46 Ras-related protein Rab-46 protein protein human Homo sapiens Human MAAPDGRVVSRPQRLGQGSGQGPKGSGACLHPLDSLEQKETQEQTSGQLVMLRKAQEFFQTCDAEGKGFIARKDMQRLHKELPLSLEELEDVFDALDADGNGYLTPQEFTTGFSHFFFSQNNPSQEDAGEQVAQRHEEKVYLSRGDEDLGDMGEDEEAQFRMLMDRLGAQKVLEDESDVKQLWLQLKKEEPHLLSNFEDFLTRIISQLQEAHEEKNELECALKRKIAAYDEEIQHLYEEMEQQIKSEKEQFLLKDTERFQARSQELEQKLLCKEQELEQLTQKQKRLEGQCTALHHDKHETKAENTKLKLTNQELARELERTSWELQDAQQQLESLQQEACKLHQEKEMEVYRVTESLQREKAGLLKQLDFLRERNKHLRDERDICFQKNKAAKANTAASRASWKKRSGSVIGKYVDSRGILRSQSEEEEEVFGIPRRSSLGLSGYPLTEEEPGTGEPGPGGPYPRPLRRIISVEEDPLPQLLDGGFEQPLSKCSEEEEVSDQGVQGQIPEAPPLKLTPTSPRGQPVGKEALCKEESSPSAPDRLFKIVFVGNSAVGKTSFLRRFCEDRFSPGMAATVGIDYRVKTLNVDNSQVALQLWDTAGQERYRCITQQFFRKADGVIVMYDLTDKQSFLSVRRWLSSVEEAVGDRVPVLLLGNKLDNEKEREVPRGLGEQLATENNLIFYECSAYSGHNTKESLLHLARFLKEQEDTVREDTIQVGHPAKKKSCCG DE746B5B604ACC44 orai3_human Protein orai-3 ORAI3 TMEM142C Transmembrane protein 142C protein protein human Homo sapiens Human MKGGEGDAGEQAPLNPEGESPAGSATYREFVHRGYLDLMGASQHSLRALSWRRLYLSRAKLKASSRTSALLSGFAMVAMVEVQLESDHEYPPGLLVAFSACTTVLVAVHLFALMVSTCLLPHIEAVSNIHNLNSVHQSPHQRLHRYVELAWGFSTALGTFLFLAEVVLVGWVKFVPIGAPLDTPTPMVPTSRVPGTLAPVATSLSPASNLPRSSASAAPSQAEPACPPRQACGGGGAHGPGWQAAMASTAIMVPVGLVFVAFALHFYRSLVAHKTDRYKQELEELNRLQGELQAV DIP protein Q9BRQ5 original sequence version: 89 DIP protein Q9BRQ5 original sequence version: 54 DIP protein Q9BRQ5 original sequence version: 55 orai2_human Protein orai-2 TMEM142B ORAI2 PP1729 C7orf19 CBCIP2 CAP-binding protein complex-interacting protein 2 Transmembrane protein 142B protein protein human Homo sapiens Human MSAELNVPIDPSAPACPEPGHKGMDYRDWVRRSYLELVTSNHHSVQALSWRKLYLSRAKLKASSRTSALLSGFAMVAMVEVQLETQYQYPRPLLIAFSACTTVLVAVHLFALLISTCILPNVEAVSNIHNLNSISESPHERMHPYIELAWGFSTVLGILLFLAEVVLLCWIKFLPVDARRQPGPPPGPGSHTGWQAALVSTIIMVPVGLIFVVFTIHFYRSLVRHKTERHNREIEELHKLKVQLDGHERSLQVL DIP protein Q96SN7 original sequence version: 92 DIP protein Q96SN7 original sequence version: 57 D53CD2376F48E23C orai1-efcab4b 3 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey association association 1c Additional proteins were indicated by silver staining, but their identity was not reported (p35, p23) Observed after store depletion of intracellular Ca2+ orai1-efcab4b-1 2 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey association association 2a Enhanced upon Ca2+store depletion efcab4b-orai1 2 9 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey association association 2a Enhanced upon Ca2+ store depletion orai1-efcab4b-2 5 9 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) direct interaction direct interaction 2b Only observed in absence of Ca2+ orai2-efcab4b 4 11 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 2c orai3-efcab4b 4 10 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 2c stim1-orai1 6 8 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) direct interaction direct interaction 2d stim1-efcab4b 6 8 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey direct interaction direct interaction 2d orai1-efcab4b-3 6 7 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey direct interaction direct interaction s7b orai1-efcab4b-4 4 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association s7c efcab4b-stim1 1 8 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component colocalization colocalization s9