IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap1. The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap1. Nat. Cell Biol. (1465-7392) 2010 imex curation Komatsu M., Kurokawa H., Waguri S., Taguchi K., Kobayashi A., Ichimura Y., Sou YS., Ueno I., Sakamoto A., Tong KI., Kim M., Nishito Y., Iemura S., Natsume T., Ueno T., Kominami E., Motohashi H., Tanaka K., Yamamoto M. Apoptosis - Interactions involving proteins with a function related to apoptosis komatsu-ms@igakuken.or.jp orchard Only protein-protein interactions Curated by Carol from InnateDB human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Komatsu M., Kurokawa H., Waguri S., Taguchi K., Kobayashi A., Ichimura Y., Sou YS., Ueno I., Sakamoto A., Tong KI., Kim M., Nishito Y., Iemura S., Natsume T., Ueno T., Kominami E., Motohashi H., Tanaka K., Yamamoto M. Apoptosis - Interactions involving proteins with a function related to apoptosis komatsu-ms@igakuken.or.jp Accepted 2011-MAR-07 by ORCHARD Nat. Cell Biol. (1465-7392) 2010 orchard Only protein-protein interactions imex curation Curated by Carol from InnateDB The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap1. The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap1. Nat. Cell Biol. (1465-7392) 2010 imex curation Komatsu M., Kurokawa H., Waguri S., Taguchi K., Kobayashi A., Ichimura Y., Sou YS., Ueno I., Sakamoto A., Tong KI., Kim M., Nishito Y., Iemura S., Natsume T., Ueno T., Kominami E., Motohashi H., Tanaka K., Yamamoto M. Apoptosis - Interactions involving proteins with a function related to apoptosis komatsu-ms@igakuken.or.jp orchard Only protein-protein interactions Curated by Carol from InnateDB in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Komatsu M., Kurokawa H., Waguri S., Taguchi K., Kobayashi A., Ichimura Y., Sou YS., Ueno I., Sakamoto A., Tong KI., Kim M., Nishito Y., Iemura S., Natsume T., Ueno T., Kominami E., Motohashi H., Tanaka K., Yamamoto M. Apoptosis - Interactions involving proteins with a function related to apoptosis komatsu-ms@igakuken.or.jp Accepted 2011-MAR-07 by ORCHARD Nat. Cell Biol. (1465-7392) 2010 orchard Only protein-protein interactions imex curation Curated by Carol from InnateDB The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap1. The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap1. Nat. Cell Biol. (1465-7392) 2010 imex curation Komatsu M., Kurokawa H., Waguri S., Taguchi K., Kobayashi A., Ichimura Y., Sou YS., Ueno I., Sakamoto A., Tong KI., Kim M., Nishito Y., Iemura S., Natsume T., Ueno T., Kominami E., Motohashi H., Tanaka K., Yamamoto M. Apoptosis - Interactions involving proteins with a function related to apoptosis komatsu-ms@igakuken.or.jp orchard Only protein-protein interactions Curated by Carol from InnateDB in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Komatsu M., Kurokawa H., Waguri S., Taguchi K., Kobayashi A., Ichimura Y., Sou YS., Ueno I., Sakamoto A., Tong KI., Kim M., Nishito Y., Iemura S., Natsume T., Ueno T., Kominami E., Motohashi H., Tanaka K., Yamamoto M. Apoptosis - Interactions involving proteins with a function related to apoptosis komatsu-ms@igakuken.or.jp Accepted 2011-MAR-07 by ORCHARD Nat. Cell Biol. (1465-7392) 2010 orchard Only protein-protein interactions imex curation Curated by Carol from InnateDB The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap1. The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap1. Nat. Cell Biol. (1465-7392) 2010 imex curation Komatsu M., Kurokawa H., Waguri S., Taguchi K., Kobayashi A., Ichimura Y., Sou YS., Ueno I., Sakamoto A., Tong KI., Kim M., Nishito Y., Iemura S., Natsume T., Ueno T., Kominami E., Motohashi H., Tanaka K., Yamamoto M. Apoptosis - Interactions involving proteins with a function related to apoptosis komatsu-ms@igakuken.or.jp orchard Only protein-protein interactions Curated by Carol from InnateDB human-huh1 Human Huh-1 cell line huh1 human hepatocellular carcinoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Komatsu M., Kurokawa H., Waguri S., Taguchi K., Kobayashi A., Ichimura Y., Sou YS., Ueno I., Sakamoto A., Tong KI., Kim M., Nishito Y., Iemura S., Natsume T., Ueno T., Kominami E., Motohashi H., Tanaka K., Yamamoto M. Apoptosis - Interactions involving proteins with a function related to apoptosis komatsu-ms@igakuken.or.jp Accepted 2011-MAR-07 by ORCHARD Nat. Cell Biol. (1465-7392) 2010 orchard Only protein-protein interactions imex curation Curated by Carol from InnateDB The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap1. The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap1. Nat. Cell Biol. (1465-7392) 2010 imex curation Komatsu M., Kurokawa H., Waguri S., Taguchi K., Kobayashi A., Ichimura Y., Sou YS., Ueno I., Sakamoto A., Tong KI., Kim M., Nishito Y., Iemura S., Natsume T., Ueno T., Kominami E., Motohashi H., Tanaka K., Yamamoto M. Apoptosis - Interactions involving proteins with a function related to apoptosis komatsu-ms@igakuken.or.jp orchard Only protein-protein interactions Curated by Carol from InnateDB in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Komatsu M., Kurokawa H., Waguri S., Taguchi K., Kobayashi A., Ichimura Y., Sou YS., Ueno I., Sakamoto A., Tong KI., Kim M., Nishito Y., Iemura S., Natsume T., Ueno T., Kominami E., Motohashi H., Tanaka K., Yamamoto M. Apoptosis - Interactions involving proteins with a function related to apoptosis komatsu-ms@igakuken.or.jp Accepted 2011-MAR-07 by ORCHARD Nat. Cell Biol. (1465-7392) 2010 orchard Only protein-protein interactions imex curation Curated by Carol from InnateDB The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap1. The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap1. Nat. Cell Biol. (1465-7392) 2010 imex curation Komatsu M., Kurokawa H., Waguri S., Taguchi K., Kobayashi A., Ichimura Y., Sou YS., Ueno I., Sakamoto A., Tong KI., Kim M., Nishito Y., Iemura S., Natsume T., Ueno T., Kominami E., Motohashi H., Tanaka K., Yamamoto M. Apoptosis - Interactions involving proteins with a function related to apoptosis komatsu-ms@igakuken.or.jp orchard Only protein-protein interactions Curated by Carol from InnateDB mouse-hepatocyte Mouse hepatocyte hepatocyte Liver primary cell A hepatocyte is a cell of the main tissue of the liver. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Komatsu M., Kurokawa H., Waguri S., Taguchi K., Kobayashi A., Ichimura Y., Sou YS., Ueno I., Sakamoto A., Tong KI., Kim M., Nishito Y., Iemura S., Natsume T., Ueno T., Kominami E., Motohashi H., Tanaka K., Yamamoto M. Apoptosis - Interactions involving proteins with a function related to apoptosis komatsu-ms@igakuken.or.jp Accepted 2011-MAR-07 by ORCHARD Nat. Cell Biol. (1465-7392) 2010 orchard Only protein-protein interactions imex curation Curated by Carol from InnateDB sqstm_mouse Sequestosome-1 Ubiquitin-binding protein p62 STONE14 Sqstm1 A170 STAP protein protein mouse Mus musculus Mouse MASFTVKAYLLGKEEATREIRRFSFCFSPEPEAEAQAAAGPGPCERLLSRVAVLFPTLRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRREHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHREHSKLIFPNPFGHLSDSFSHSRWLRKLKHGHFGWPGWEMGPPGNWSPRPPRAGDGRPCPTAESASAPPEDPNVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPTTPESSSTGTEDKSNTQPSSCSSEVSKPDGAGEGPAQSLTEQMKKIALESVGQPEEQMESGNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL DIP protein Q64337 original sequence version: 121 DIP protein Q64337 original sequence version: 130 ED4CCCA7741D35CA keap1_mouse Kelch-like ECH-associated protein 1 Cytosolic inhibitor of Nrf2 Keap1 Inrf2 Kiaa0132 protein protein mouse Mus musculus Mouse MQPEPKLSGAPRSSQFLPLWSKCPEGAGDAVMYASTECKAEVTPSQDGNRTFSYTLEDHTKQAFGVMNELRLSQQLCDVTLQVKYEDIPAAQFMAHKVVLASSSPVFKAMFTNGLREQGMEVVSIEGIHPKVMERLIEFAYTASISVGEKCVLHVMNGAVMYQIDSVVRACSDFLVQQLDPSNAIGIANFAEQIGCTELHQRAREYIYMHFGEVAKQEEFFNLSHCQLATLISRDDLNVRCESEVFHACIDWVKYDCPQRRFYVQALLRAVRCHALTPRFLQTQLQKCEILQADARCKDYLVQIFQELTLHKPTQAVPCRAPKVGRLIYTAGGYFRQSLSYLEAYNPSNGSWLRLADLQVPRSGLAGCVVGGLLYAVGGRNNSPDGNTDSSALDCYNPMTNQWSPCASMSVPRNRIGVGVIDGHIYAVGGSHGCIHHSSVERYEPERDEWHLVAPMLTRRIGVGVAVLNRLLYAVGGFDGTNRLNSAECYYPERNEWRMITPMNTIRSGAGVCVLHNCIYAAGGYDGQDQLNSVERYDVETETWTFVAPMRHHRSALGITVHQGKIYVLGGYDGHTFLDSVECYDPDSDTWSEVTRMTSGRSGVGVAVTMEPCRKQIDQQNCTC DIP protein Q9Z2X8 original sequence version: 104 4645DB0122FB5F54 sqstm_human Sequestosome-1 SQSTM1 ORCA OSIL Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa Ubiquitin-binding protein p62 EBI3-associated protein of 60 kDa protein protein human Homo sapiens Human MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL DIP protein Q13501 original sequence version: 139 DIP protein Q13501 original sequence version: 142 DIP protein Q13501 original sequence version: 163 DIP protein Q13501 original sequence version: 146 DIP protein Q13501 original sequence version: 162 DIP protein Q13501 original sequence version: 155 DIP protein Q13501 original sequence version: 140 462D94C171F337CD keap1_human Kelch-like ECH-associated protein 1 Cytosolic inhibitor of Nrf2 Kelch-like protein 19 KEAP1 INRF2 KIAA0132 KLHL19 protein protein human Homo sapiens Human MQPDPRPSGAGACCRFLPLQSQCPEGAGDAVMYASTECKAEVTPSQHGNRTFSYTLEDHTKQAFGIMNELRLSQQLCDVTLQVKYQDAPAAQFMAHKVVLASSSPVFKAMFTNGLREQGMEVVSIEGIHPKVMERLIEFAYTASISMGEKCVLHVMNGAVMYQIDSVVRACSDFLVQQLDPSNAIGIANFAEQIGCVELHQRAREYIYMHFGEVAKQEEFFNLSHCQLVTLISRDDLNVRCESEVFHACINWVKYDCEQRRFYVQALLRAVRCHSLTPNFLQMQLQKCEILQSDSRCKDYLVKIFEELTLHKPTQVMPCRAPKVGRLIYTAGGYFRQSLSYLEAYNPSDGTWLRLADLQVPRSGLAGCVVGGLLYAVGGRNNSPDGNTDSSALDCYNPMTNQWSPCAPMSVPRNRIGVGVIDGHIYAVGGSHGCIHHNSVERYEPERDEWHLVAPMLTRRIGVGVAVLNRLLYAVGGFDGTNRLNSAECYYPERNEWRMITAMNTIRSGAGVCVLHNCIYAAGGYDGQDQLNSVERYDVETETWTFVAPMKHRRSALGITVHQGRIYVLGGYDGHTFLDSVECYDPDTDTWSEVTRMTSGRSGVGVAVTMEPCRKQIDQQNCTC DIP protein Q14145 original sequence version: 107 DIP protein Q14145 original sequence version: 138 DIP protein Q14145 original sequence version: 153 DIP protein Q14145 original sequence version: 106 DIP protein Q14145 original sequence version: 163 CE180F3897BB8C97 nf2l2_human Nuclear factor erythroid 2-related factor 2 NFE2L2 NRF2 Nuclear factor, erythroid derived 2, like 2 HEBP1 protein protein human Homo sapiens Human MMDLELPPPGLPSQQDMDLIDILWRQDIDLGVSREVFDFSQRRKEYELEKQKKLEKERQEQLQKEQEKAFFAQLQLDEETGEFLPIQPAQHIQSETSGSANYSQVAHIPKSDALYFDDCMQLLAQTFPFVDDNEVSSATFQSLVPDIPGHIESPVFIATNQAQSPETSVAQVAPVDLDGMQQDIEQVWEELLSIPELQCLNIENDKLVETTMVPSPEAKLTEVDNYHFYSSIPSMEKEVGNCSPHFLNAFEDSFSSILSTEDPNQLTVNSLNSDATVNTDFGDEFYSAFIAEPSISNSMPSPATLSHSLSELLNGPIDVSDLSLCKAFNQNHPESTAEFNDSDSGISLNTSPSVASPEHSVESSSYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSSGDMVQPLSPSQGQSTHVHDAQCENTPEKELPVSPGHRKTPFTKDKHSSRLEAHLTRDELRAKALHIPFPVEKIINLPVVDFNEMMSKEQFNEAQLALIRDIRRRGKNKVAAQNCRKRKLENIVELEQDLDHLKDEKEKLLKEKGENDKSLHLLKKQLSTLYLEVFSMLRDEDGKPYSPSEYSLQQTRDGNVFLVPKSKKPDVKKN DIP protein Q16236 original sequence version: 80 DIP protein Q16236 original sequence version: 103 DIP protein Q16236 original sequence version: 148 DIP protein Q16236 original sequence version: 102 DIP protein Q16236-1 original sequence version: 113 99FAFD811B6C1416 keap1-sqstm1-1 1 8 unspecified role unspecified role bait bait Q9Z2X8:p.Ser508Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A Wed May 02 19:16:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser508ala' Tue Jun 30 17:15:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser508Ala' region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q9Z2X8:p.Ser555Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A Wed May 02 20:31:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser555ala' Tue Jun 30 19:58:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser555Ala' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9Z2X8:p.Tyr334Ala mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 15:00:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr334ala' Tue Jun 30 17:52:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr334Ala' Q9Z2X8:p.Asn382Ala mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain N A Wed May 02 20:13:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn382ala' Tue Jun 30 19:24:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn382Ala' Q9Z2X8:p.Arg380Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A Wed May 02 15:26:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg380ala' Tue Jun 30 19:43:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg380Ala' Q9Z2X8:p.Gln530Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 18:42:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln530ala' Tue Jun 30 18:06:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln530Ala' Q9Z2X8:p.Ser363Ala mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A Wed May 02 18:25:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser363ala' Tue Jun 30 16:02:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser363Ala' Q9Z2X8:p.Ser602Ala mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A Wed May 02 17:13:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser602ala' Tue Jun 30 15:46:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser602Ala' Q9Z2X8:p.Arg415Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A Wed May 02 15:15:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg415ala' Tue Jun 30 20:16:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg415Ala' 9 unspecified role unspecified role prey prey physical association physical association Fig. 1A, S3D, 3B keap1-nfe2l2 1 8 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9Z2X8:p.Ser555Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:25:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser555ala' Tue Jun 30 17:34:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser555Ala' Q9Z2X8:p.Tyr334Ala mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 15:15:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr334ala' Tue Jun 30 16:33:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr334Ala' Q9Z2X8:p.Ser363Ala mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A Wed May 02 17:30:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser363ala' Tue Jun 30 18:48:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser363Ala' Q9Z2X8:p.Asn382Ala mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain N A Wed May 02 14:46:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn382ala' Tue Jun 30 18:14:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn382Ala' Q9Z2X8:p.Ser602Ala mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A Wed May 02 17:25:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser602ala' Tue Jun 30 18:50:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser602Ala' Q9Z2X8:p.Arg380Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:34:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg380ala' Tue Jun 30 19:25:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg380Ala' Q9Z2X8:p.Arg415Ala mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A Wed May 02 17:40:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg415ala' Tue Jun 30 17:35:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg415Ala' Q9Z2X8:p.Gln530Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 15:45:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln530ala' Tue Jun 30 19:14:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln530Ala' Q9Z2X8:p.Ser508Ala mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A Wed May 02 16:27:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser508ala' Tue Jun 30 18:43:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser508Ala' 11 unspecified role unspecified role prey prey physical association physical association Fig. 1A, S3D, 3B sqstm1-keap1-2 1 10 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q64337:p.Thr352Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A Wed May 02 19:31:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr352ala' Tue Jun 30 15:38:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr352Ala' region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q64337:p.Glu354Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A Wed May 02 14:44:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu354ala' Tue Jun 30 18:26:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu354Ala' Q64337:p.Asp349Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A Wed May 02 17:25:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp349ala' Tue Jun 30 19:40:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp349Ala' Q64337:p.Ser351Ala mutation increasing mutation increasing interaction mutation increasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A Wed May 02 17:32:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser351ala' Tue Jun 30 16:13:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser351Ala' Q64337:p.Pro350Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain P A Wed May 02 17:38:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro350ala' Tue Jun 30 19:02:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro350Ala' physical association physical association Fig. S3B keap1-sqstm1-7 1 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3B. sqstm1-keap1-3 5 8 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q64337:p.Glu354Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A Wed May 02 19:01:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu354ala' Tue Jun 30 16:43:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu354Ala' Q64337:p.Asp349Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A Wed May 02 16:53:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp349ala' Tue Jun 30 16:01:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp349Ala' Q64337:p.Thr352Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A Wed May 02 20:18:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr352ala' Tue Jun 30 17:37:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr352Ala' Q64337:p.Thr352Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A Wed May 02 19:31:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr352ala' Tue Jun 30 16:50:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr352Ala' Q64337:p.Pro350Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain P A Wed May 02 15:16:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro350ala' Tue Jun 30 16:48:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro350Ala' region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1B, S2, S3A keap1-sqstm-3 5 7 unspecified role unspecified role prey prey region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role bait bait Q9Z2X8:p.Ser363Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:31:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser363ala' Tue Jun 30 19:44:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser363Ala' Q9Z2X8:p.Arg415Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A Wed May 02 14:31:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg415ala' Tue Jun 30 17:36:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg415Ala' Q9Z2X8:p.Tyr334Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 17:10:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr334ala' Tue Jun 30 18:23:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr334Ala' Q9Z2X8:p.Asn382Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain N A Wed May 02 15:51:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn382ala' Tue Jun 30 18:27:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn382Ala' Q9Z2X8:p.Ser508Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:25:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser508ala' Tue Jun 30 17:00:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser508Ala' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9Z2X8:p.Ser602Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:46:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser602ala' Tue Jun 30 17:08:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser602Ala' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. S3C sqstm1-keap1-4 6 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 1D. Endogenous sqstm1 was also present in the interaction. keap1-sqstm1-4 4 9 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association Fig. 1E. keap1-sqstm1-5 2 7 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain in vitro In vitro 8 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 2. Table S1 2.8A keap1 forms a six-bladed beta-propeller structure with pseudo six-fold symmetry, the sqstm peptide (aa346-359) binds to the bottom of the beta-propeller structure. R-working:17.8% R-free:23.2% keap1-sqstm1-6 3 7 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3A. Ka=(5.4 ± 0.3) x 10E5 M-1 sqstm1-keap1-5 1 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey physical association physical association Fig. 1D sqstm1-keap1-6 4 9 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Fig. 1E.