IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Identification and characterization of unique proline-rich peptides binding to the mitochondrial fission protein hFis1. Identification and characterization of unique proline-rich peptides binding to the mitochondrial fission protein hFis1. J. Biol. Chem. (0021-9258) 2010 imex curation Serasinghe M.N., Seneviratne A.M., Smrcka A.V., Yoon Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Anti-M13 antibodies were used for the detection of the prey. yisang_yoon@urmc.rochester.edu 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro phage display phage display elisa enzyme linked immunosorbent assay ELISA ELISA elisa Serasinghe M.N., Seneviratne A.M., Smrcka A.V., Yoon Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Anti-M13 antibodies were used for the detection of the prey. yisang_yoon@urmc.rochester.edu Accepted 2011-SEP-26 by ORCHARD 21-Dec-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation Identification and characterization of unique proline-rich peptides binding to the mitochondrial fission protein hFis1. Identification and characterization of unique proline-rich peptides binding to the mitochondrial fission protein hFis1. J. Biol. Chem. (0021-9258) 2010 imex curation Serasinghe M.N., Seneviratne A.M., Smrcka A.V., Yoon Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Anti-M13 antibodies were used for the detection of the prey. yisang_yoon@urmc.rochester.edu 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Serasinghe M.N., Seneviratne A.M., Smrcka A.V., Yoon Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Anti-M13 antibodies were used for the detection of the prey. yisang_yoon@urmc.rochester.edu Accepted 2011-SEP-26 by ORCHARD 21-Dec-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation Identification and characterization of unique proline-rich peptides binding to the mitochondrial fission protein hFis1. Identification and characterization of unique proline-rich peptides binding to the mitochondrial fission protein hFis1. J. Biol. Chem. (0021-9258) 2010 imex curation Serasinghe M.N., Seneviratne A.M., Smrcka A.V., Yoon Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Anti-M13 antibodies were used for the detection of the prey. yisang_yoon@urmc.rochester.edu 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro phage display phage display nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Serasinghe M.N., Seneviratne A.M., Smrcka A.V., Yoon Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Anti-M13 antibodies were used for the detection of the prey. yisang_yoon@urmc.rochester.edu Accepted 2011-SEP-26 by ORCHARD J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation These versions of the interacting peptides were generated by phage display, so the cell machinery generated them using the gene information provided by the phage. They were not chemically synthesized, then, that's why I used Bpm13 as the origin for these. Identification and characterization of unique proline-rich peptides binding to the mitochondrial fission protein hFis1. Identification and characterization of unique proline-rich peptides binding to the mitochondrial fission protein hFis1. J. Biol. Chem. (0021-9258) 2010 imex curation Serasinghe M.N., Seneviratne A.M., Smrcka A.V., Yoon Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Anti-M13 antibodies were used for the detection of the prey. yisang_yoon@urmc.rochester.edu 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Serasinghe M.N., Seneviratne A.M., Smrcka A.V., Yoon Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Anti-M13 antibodies were used for the detection of the prey. yisang_yoon@urmc.rochester.edu Accepted 2011-SEP-26 by ORCHARD 21-Dec-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation ph1 Peptide 1 group 1, expressed in M13 phage. PMID: 19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro QHTPHPLPPFLI fis1_human Mitochondrial fission 1 protein FIS1 TTC11 FIS1 homolog Tetratricopeptide repeat protein 11 CGI-135 protein protein human Homo sapiens Human MEAVLNELVSVEDLLKFEKKFQSEKAAGSVSKSTQFEYAWCLVRSKYNDDIRKGIVLLEELLPKGSKEEQRDYVFYLAVGNYRLKEYEKALKYVRGLLQTEPQNNQAKELERLIDKAMKKDGLVGMAIVGGMALGVAGLAGLIGLAVSKSKS 6E76EC02B3731A9B ph8 Peptide 8, group 2, expressed in phage M13. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro ESSWQDMLYLLR ph7 peptide 7, group 2, expressed in M13 phage. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro HLWEHEMILDLL pep2 peptide 2, group 1, chemically synthesized. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) SHKQDPLPWPRF ph2 peptide 2, group 1, expressed in M13 phage. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro SHKQDPLPWPRF ph4 Peptide 4, group 1, expressed in phage M13. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro STPNPLPRWLVP ph3 Peptide 3, group 3, expressed in phage M13. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro WKTQTHPFWDDL ph5 Peptide 5, group 1, expressed in phage M13. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro KVQLPLPYPFPF ph9 Peptide 9, group 3, expressed in phage M13. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro SGIHAYPHWLDH ph13 Peptide 13, group 1, expressed in phage M13. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro KHDPLPYPHFLL pep4 Peptide 4, group 1, chemically synthesized. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) STPNPLPRWLVP ph10 Peptide 10, group 2, expressed in phage M13. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro THRWQEELTLLL pep13 peptide 13, group 1, chemically synthesized. PMID:19864424 peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) KHDPLPYPHFLL pep1 Peptide 1, group 1. PMID:19864624 peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) QHTPHPLPPFLI fis1-ph1-1 3 5 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. 6 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 1b fis1-ph2-1 3 10 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. 6 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. ecoli Escherichia coli (strain K12) direct interaction direct interaction 1b fis1-ph3 3 12 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. 6 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. ecoli Escherichia coli (strain K12) direct interaction direct interaction 1b fis1-ph4 3 6 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. direct interaction direct interaction 1b fis1-ph5 3 13 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. 6 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. ecoli Escherichia coli (strain K12) direct interaction direct interaction 1b fis1-ph13 3 15 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. 6 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. ecoli Escherichia coli (strain K12) direct interaction direct interaction 1b fis1-ph7 3 8 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. bpm13 Enterobacteria phage M13 Bacteriophage M13 6 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. ecoli Escherichia coli (strain K12) direct interaction direct interaction 1b fis1-ph8 3 7 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. bpm13 Enterobacteria phage M13 Bacteriophage M13 6 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. ecoli Escherichia coli (strain K12) direct interaction direct interaction 1b fis1-ph10 3 17 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. bpm13 Enterobacteria phage M13 Bacteriophage M13 6 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. ecoli Escherichia coli (strain K12) direct interaction direct interaction 1b fis1-ph9-1 3 6 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. bpm13 Enterobacteria phage M13 Bacteriophage M13 direct interaction direct interaction 1b fis1-ph1-1 1 6 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytosolic region without alpha-1 domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. Q9Y3D6:p.Leu42Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 18:07:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu42pro' Tue Jun 30 16:00:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu42Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu58Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 15:33:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu58pro' Tue Jun 30 18:06:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu58Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu91Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 19:54:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu91pro' Tue Jun 30 16:37:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu91Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu77Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 14:28:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu77pro' Tue Jun 30 15:44:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu77Pro' Mutation performed on the hFis1[32-122] form. ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. direct interaction direct interaction 1c, 1d, 2a and 2b fis1-ph3-2 1 6 unspecified role unspecified role bait bait purified purified Q9Y3D6:p.Leu91Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 16:43:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu91pro' Tue Jun 30 17:03:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu91Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu42Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 15:34:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu42pro' Tue Jun 30 16:14:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu42Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu77Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 18:14:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu77pro' Tue Jun 30 17:22:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu77Pro' Mutation performed on the hFis1[32-122] form. cytosolic region without alpha-1 domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. Q9Y3D6:p.Leu58Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 18:48:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu58pro' Tue Jun 30 16:51:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu58Pro' Mutation performed on the hFis1[32-122] form. ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. direct interaction direct interaction 1c, 1d, 2a and 2b fis1-ph4-2 1 11 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. 6 unspecified role unspecified role bait bait purified purified Q9Y3D6:p.Leu77Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 15:31:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu77pro' Tue Jun 30 16:50:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu77Pro' Q9Y3D6:p.Leu77Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 18:00:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu77pro' Tue Jun 30 18:25:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu77Pro' Mutation performed on the hFis1[32-122] form. cytosolic region without alpha-1 domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9Y3D6:p.Leu42Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 16:47:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu42pro' Tue Jun 30 16:42:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu42Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu58Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 15:57:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu58pro' Tue Jun 30 18:21:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu58Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu91Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 18:51:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu91pro' Tue Jun 30 19:09:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu91Pro' Mutation performed on the hFis1[32-122] form. ecoli Escherichia coli (strain K12) direct interaction direct interaction 1c, 1d, 2a and 2b fis1-ph9-3 1 6 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytosolic region without alpha-1 domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. Q9Y3D6:p.Leu58Pro mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 17:33:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu58pro' Tue Jun 30 20:23:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu58Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu42Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 16:05:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu42pro' Tue Jun 30 17:48:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu42Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu91Pro mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 15:43:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu91pro' Tue Jun 30 20:08:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu91Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu77Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 15:23:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu77pro' Tue Jun 30 17:31:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu77Pro' Mutation performed on the hFis1[32-122] form. ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. bpm13 Enterobacteria phage M13 Bacteriophage M13 direct interaction direct interaction 1c, 1d, 2a and 2b The units of the EC50 parameter are number of phage units. fis1-ph2-3 1 10 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. 6 unspecified role unspecified role bait bait purified purified Q9Y3D6:p.Leu58Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 18:16:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu58pro' Tue Jun 30 17:03:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu58Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu77Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 18:32:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu77pro' Tue Jun 30 19:46:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu77Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu91Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 16:07:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu91pro' Tue Jun 30 16:20:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu91Pro' Mutation performed on the hFis1[32-122] form. his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytosolic region without alpha-1 domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q9Y3D6:p.Leu42Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 17:08:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu42pro' Tue Jun 30 16:07:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu42Pro' Mutation performed on the hFis1[32-122] form. ecoli Escherichia coli (strain K12) direct interaction direct interaction 1c, 1d, 2a and 2b fis1-ph5-2 1 6 unspecified role unspecified role prey prey purified purified Q9Y3D6:p.Leu91Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 14:54:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu91pro' Tue Jun 30 17:46:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu91Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu58Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 17:16:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu58pro' Tue Jun 30 19:52:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu58Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu42Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 15:23:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu42pro' Tue Jun 30 18:16:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu42Pro' Mutation performed on the hFis1[32-122] form. myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytosolic region without alpha-1 domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. Q9Y3D6:p.Leu77Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 18:45:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu77pro' Tue Jun 30 16:29:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu77Pro' Mutation performed on the hFis1[32-122] form. bpm13 Enterobacteria phage M13 Bacteriophage M13 13 unspecified role unspecified role bait bait infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. direct interaction direct interaction 1c, 1d, 2a and 2b fis1-ph13-2 1 6 unspecified role unspecified role bait bait purified purified Q9Y3D6:p.Leu42Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 18:32:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu42pro' Tue Jun 30 16:42:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu42Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu58Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 16:17:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu58pro' Tue Jun 30 19:53:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu58Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu77Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 17:57:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu77pro' Tue Jun 30 16:20:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu77Pro' Mutation performed on the hFis1[32-122] form. his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. cytosolic region without alpha-1 domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9Y3D6:p.Leu91Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 14:51:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu91pro' Tue Jun 30 18:29:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu91Pro' Mutation performed on the hFis1[32-122] form. ecoli Escherichia coli (strain K12) 15 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. direct interaction direct interaction 1c, 1d, 2a and 2b The units of the EC50 parameter are number of phage units. fis1-ph8-2 1 6 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. cytosolic region without alpha-1 domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q9Y3D6:p.Leu77Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 15:17:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu77pro' Tue Jun 30 20:21:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu77Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu91Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 18:49:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu91pro' Tue Jun 30 17:14:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu91Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu42Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 18:20:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu42pro' Tue Jun 30 16:58:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu42Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu58Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 20:23:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu58pro' Tue Jun 30 17:38:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu58Pro' Mutation performed on the hFis1[32-122] form. ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. bpm13 Enterobacteria phage M13 Bacteriophage M13 direct interaction direct interaction 1c, 1d, 2a and 2b The units of the EC50 parameter are number of phage units. fis1-ph7-2 1 8 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. bpm13 Enterobacteria phage M13 Bacteriophage M13 6 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytosolic region without alpha-1 domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q9Y3D6:p.Leu42Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 18:20:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu42pro' Tue Jun 30 18:25:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu42Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu77Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 19:23:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu77pro' Tue Jun 30 19:52:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu77Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu58Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 15:27:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu58pro' Tue Jun 30 19:58:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu58Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu91Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 19:03:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu91pro' Tue Jun 30 17:53:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu91Pro' Mutation performed on the hFis1[32-122] form. ecoli Escherichia coli (strain K12) direct interaction direct interaction 1c, 1d, 2a and 2b fis1-ph10-2 1 17 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. bpm13 Enterobacteria phage M13 Bacteriophage M13 6 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. cytosolic region without alpha-1 domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9Y3D6:p.Leu42Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 20:02:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu42pro' Tue Jun 30 19:19:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu42Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu77Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 15:11:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu77pro' Tue Jun 30 19:01:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu77Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu91Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 18:28:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu91pro' Tue Jun 30 19:47:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu91Pro' Mutation performed on the hFis1[32-122] form. Q9Y3D6:p.Leu58Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 18:56:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu58pro' Tue Jun 30 18:36:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu58Pro' Mutation performed on the hFis1[32-122] form. ecoli Escherichia coli (strain K12) direct interaction direct interaction 1c, 1d, 2a and 2b fis1-ph10-4 1 6 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. ecoli Escherichia coli (strain K12) 17 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. bpm13 Enterobacteria phage M13 Bacteriophage M13 9 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 3b fis1-ph4-4 1 9 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. 6 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3b fis1-ph7-4 1 9 competitor competitor competitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. bpm13 Enterobacteria phage M13 Bacteriophage M13 physical association physical association 3b fis1-ph1-3 1 5 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. 6 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 competitor competitor competitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 3b fis1-ph3-4 1 9 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. direct interaction direct interaction 3b fis1-ph13-4 1 9 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. ecoli Escherichia coli (strain K12) 15 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. direct interaction direct interaction 3b The units of the EC50 parameter are number of phage units. fis1-ph5-3 1 9 competitor competitor competitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. 6 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. bpm13 Enterobacteria phage M13 Bacteriophage M13 physical association physical association 3b fis1-ph8-4 1 9 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. bpm13 Enterobacteria phage M13 Bacteriophage M13 direct interaction direct interaction 3b The units of the EC50 parameter are number of phage units. fis1-ph2-5 1 10 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. 6 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. ecoli Escherichia coli (strain K12) 9 inhibitor inhibitor unspecified role unspecified role purified purified pep2:p.Leu7Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Wed May 02 14:52:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu7ala' Tue Jun 30 16:55:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu7Ala' This mutation abrogates the competitive inhibition action of this peptide in the fi1-ph2 interaction. pep2:p.Pro8Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P A Wed May 02 15:50:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro8ala' Tue Jun 30 17:26:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro8Ala' This mutation greatly diminishes the competitive inhibition action of this peptide in the fi1-ph2 interaction. chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 3a, 3b, 4b, 4e and 4f fis1-ph7-6 1 8 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. bpm13 Enterobacteria phage M13 Bacteriophage M13 6 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 18 competitor competitor competitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 3c fis1-ph1-5 1 5 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. 6 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 18 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 3c fis1-ph5-4 1 13 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. 6 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. bpm13 Enterobacteria phage M13 Bacteriophage M13 18 competitor competitor competitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 3b fis1-ph2-7 1 10 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. 6 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 18 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 3c fis1-ph10-6 1 18 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 17 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. bpm13 Enterobacteria phage M13 Bacteriophage M13 6 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. ecoli Escherichia coli (strain K12) direct interaction direct interaction 3c fis1-ph4-6 1 11 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. 6 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 18 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 3c fis1-ph3-6 1 18 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 12 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. 6 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 3c fis1-ph13-6 1 6 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. ecoli Escherichia coli (strain K12) 15 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. 18 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 4d and 4e The units of the EC50 parameter are number of phage units. fis1-ph8-6 1 18 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. bpm13 Enterobacteria phage M13 Bacteriophage M13 6 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. ecoli Escherichia coli (strain K12) direct interaction direct interaction 3c The units of the EC50 parameter are number of phage units. fis1-ph1-7 1 19 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. 6 unspecified role unspecified role bait bait purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. ecoli Escherichia coli (strain K12) direct interaction direct interaction 4a and 4e fis1-ph4-8 1 16 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey infection infection piii coat protein fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range bpm13 Enterobacteria phage M13 Bacteriophage M13 Peptide expressed in M13 phage, fused with a minor coat protein (pIII) that is exposed in the virions. direct interaction direct interaction 4c and 4e fis1-pep1 4 6 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 19 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 5a and 5k fis1-pep2 4 6 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain Q9Y3D6:p.Leu42Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 19:12:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu42pro' Tue Jun 30 16:13:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu42Pro' ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey purified purified pep2:p.Leu7Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Wed May 02 14:44:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu7ala' Tue Jun 30 15:42:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu7Ala' chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 5b, 5e, 5g and 5k fis1-pep4 4 16 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role bait bait purified purified cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. ecoli Escherichia coli (strain K12) direct interaction direct interaction 5c and 5k fis1-pep13 4 6 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain Q9Y3D6:p.Leu42Pro mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 17:39:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu42pro' Tue Jun 30 19:12:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu42Pro' ecoli Escherichia coli (strain K12) 18 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 5d, 5k and 5h fis1-pep13-1 4 6 unspecified role unspecified role bait bait purified purified cytosolic region experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 18 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 5j and 5k fis1-pep2-2 4 9 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. cytosolic region experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 5i and 5k fis1-pep2-4 2 6 unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 5f fis1-pep13-3 2 18 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component purified purified myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytosolic region without alpha-1 domain experimental feature experimental feature certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Upstream myc tag. ecoli Escherichia coli (strain K12) direct interaction direct interaction 5f