UniProt UniProt http://www.uniprot.org Solution structure of NOD1 CARD and mutational analysis of its interaction with the CARD of downstream kinase RICK. Solution structure of NOD1 CARD and mutational analysis of its interaction with the CARD of downstream kinase RICK. J. Mol. Biol. (0022-2836) 2007 imex curation Manon F., Favier A., Nunez G., Simorre JP., Cusack S. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of RIPK2 inferred by mutant mapping cusack@embl-grenoble.fr orchard Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Manon F., Favier A., Nunez G., Simorre JP., Cusack S. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of RIPK2 inferred by mutant mapping cusack@embl-grenoble.fr Accepted 2011-MAR-23 by ORCHARD J. Mol. Biol. (0022-2836) 2007 orchard Only protein-protein interactions imex curation nod1_human Nucleotide-binding oligomerization domain-containing protein 1 Caspase recruitment domain-containing protein 4 NOD1 CARD4 protein protein human Homo sapiens Human MEEQGHSEMEIIPSESHPHIQLLKSNRELLVTHIRNTQCLVDNLLKNDYFSAEDAEIVCACPTQPDKVRKILDLVQSKGEEVSEFFLYLLQQLADAYVDLRPWLLEIGFSPSLLTQSKVVVNTDPVSRYTQQLRHHLGRDSKFVLCYAQKEELLLEEIYMDTIMELVGFSNESLGSLNSLACLLDHTTGILNEQGETIFILGDAGVGKSMLLQRLQSLWATGRLDAGVKFFFHFRCRMFSCFKESDRLCLQDLLFKHYCYPERDPEEVFAFLLRFPHVALFTFDGLDELHSDLDLSRVPDSSCPWEPAHPLVLLANLLSGKLLKGASKLLTARTGIEVPRQFLRKKVLLRGFSPSHLRAYARRMFPERALQDRLLSQLEANPNLCSLCSVPLFCWIIFRCFQHFRAAFEGSPQLPDCTMTLTDVFLLVTEVHLNRMQPSSLVQRNTRSPVETLHAGRDTLCSLGQVAHRGMEKSLFVFTQEEVQASGLQERDMQLGFLRALPELGPGGDQQSYEFFHLTLQAFFTAFFLVLDDRVGTQELLRFFQEWMPPAGAATTSCYPPFLPFQCLQGSGPAREDLFKNKDHFQFTNLFLCGLLSKAKQKLLRHLVPAAALRRKRKALWAHLFSSLRGYLKSLPRVQVESFNQVQAMPTFIWMLRCIYETQSQKVGQLAARGICANYLKLTYCNACSADCSALSFVLHHFPKRLALDLDNNNLNDYGVRELQPCFSRLTVLRLSVNQITDGGVKVLSEELTKYKIVTYLGLYNNQITDVGARYVTKILDECKGLTHLKLGKNKITSEGGKYLALAVKNSKSISEVGMWGNQVGDEGAKAFAEALRNHPSLTTLSLASNGISTEGGKSLARALQQNTSLEILWLTQNELNDEVAESLAEMLKVNQTLKHLWLIQNQITAKGTAQLADALQSNTGITEICLNGNLIKPEEAKVYEDEKRIICF DIP protein Q9Y239 original sequence version: 140 DIP protein Q9Y239 original sequence version: 99 DIP protein Q9Y239 original sequence version: 105 0A9DF5FC6487E21A ripk2_human Receptor-interacting serine/threonine-protein kinase 2 RIPK2 CARDIAK RICK RIP2 UNQ277/PRO314/PRO34092 RIP-like-interacting CLARP kinase Receptor-interacting protein 2 CARD-containing interleukin-1 beta-converting enzyme-associated kinase Tyrosine-protein kinase RIPK2 protein protein human Homo sapiens Human MNGEAICSALPTIPYHKLADLRYLSRGASGTVSSARHADWRVQVAVKHLHIHTPLLDSERKDVLREAEILHKARFSYILPILGICNEPEFLGIVTEYMPNGSLNELLHRKTEYPDVAWPLRFRILHEIALGVNYLHNMTPPLLHHDLKTQNILLDNEFHVKIADFGLSKWRMMSLSQSRSSKSAPEGGTIIYMPPENYEPGQKSRASIKHDIYSYAVITWEVLSRKQPFEDVTNPLQIMYSVSQGHRPVINEESLPYDIPHRARMISLIESGWAQNPDERPSFLKCLIELEPVLRTFEEITFLEAVIQLKKTKLQSVSSAIHLCDKKKMELSLNIPVNHGPQEESCGSSQLHENSGSPETSRSLPAPQDNDFLSRKAQDCYFMKLHHCPGNHSWDSTISGSQRAAFCDHKTTPCSSAIINPLSTAGNSERLQPGIAQQWIQSKREDIVNQMTEACLNQSLDALLSRDLIMKEDYELVSTKPTRTSKVRQLLDTTDIQGEEFAKVIVQKLKDNKQMGLQPYPEILVVSRSPSLNLLQNKSM DIP protein O43353 original sequence version: 159 DIP protein O43353-1 original sequence version: 116 DIP protein O43353 original sequence version: 116 575A692239505792 ripk2-nod1 1 3 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O43353:p.[Arg444Glu;Arg483Glu;Arg488Glu] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R E certain certain sequence position certain certain certain sequence position certain R E certain certain sequence position certain certain certain sequence position certain R E Fri Jul 22 11:03:36 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg488glu,arg483glu,arg488glu' Wed May 02 16:54:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg444glu,arg483glu,arg488glu' Tue Jun 30 18:02:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg444Glu;Arg483Glu;Arg488Glu]' O43353:p.Arg444Glu mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R E Wed May 02 17:28:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg444glu' Tue Jun 30 19:54:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg444Glu' O43353:p.[Arg444Glu;Arg483Glu] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R E certain certain sequence position certain certain certain sequence position certain R E Wed May 02 16:05:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg444glu,arg483glu' Tue Jun 30 19:44:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg444Glu;Arg483Glu]' 2 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9Y239:p.Glu53Lys mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E K Wed May 02 17:14:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu53lys' Tue Jun 30 18:40:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu53Lys' Q9Y239:p.Glu56Lys mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E K Wed May 02 17:33:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu56lys' Tue Jun 30 17:08:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu56Lys' Q9Y239:p.Arg69Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R E Wed May 02 15:29:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg69glu' Tue Jun 30 17:37:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg69Glu' Q9Y239:p.Asp54Lys mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D K Wed May 02 19:42:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp54lys' Tue Jun 30 18:46:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp54Lys' Q9Y239:p.Val41Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V A Wed May 02 20:03:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val41ala' Tue Jun 30 20:08:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val41Ala' physical association physical association fig 6