UniProt UniProt http://www.uniprot.org Role of histone H2A ubiquitination in Polycomb silencing. Role of histone H2A ubiquitination in Polycomb silencing. Nature (0028-0836) 2004 imex curation Wang H., Wang L., Erdjument-Bromage H., Vidal M., Tempst P., Jones RS., Zhang Y. H2A ubiquitination assay not included, H2A identity cannot be established (fig3b) yi_zhang@med.unc.edu 26-Sep-2011: Contacted by JYOTI. ORCHARD Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Wang H., Wang L., Erdjument-Bromage H., Vidal M., Tempst P., Jones RS., Zhang Y. H2A ubiquitination assay not included, H2A identity cannot be established (fig3b) yi_zhang@med.unc.edu Accepted 2011-JUL-11 by ORCHARD 26-Sep-2011: Contacted by JYOTI. Nature (0028-0836) 2004 ORCHARD Only protein-protein interactions imex curation Role of histone H2A ubiquitination in Polycomb silencing. Role of histone H2A ubiquitination in Polycomb silencing. Nature (0028-0836) 2004 imex curation Wang H., Wang L., Erdjument-Bromage H., Vidal M., Tempst P., Jones RS., Zhang Y. H2A ubiquitination assay not included, H2A identity cannot be established (fig3b) yi_zhang@med.unc.edu 26-Sep-2011: Contacted by JYOTI. ORCHARD Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom western blot western blot Immuno blot Immuno blot Wang H., Wang L., Erdjument-Bromage H., Vidal M., Tempst P., Jones RS., Zhang Y. H2A ubiquitination assay not included, H2A identity cannot be established (fig3b) yi_zhang@med.unc.edu Accepted 2011-JUL-11 by ORCHARD 26-Sep-2011: Contacted by JYOTI. Nature (0028-0836) 2004 ORCHARD Only protein-protein interactions imex curation Role of histone H2A ubiquitination in Polycomb silencing. Role of histone H2A ubiquitination in Polycomb silencing. Nature (0028-0836) 2004 imex curation Wang H., Wang L., Erdjument-Bromage H., Vidal M., Tempst P., Jones RS., Zhang Y. H2A ubiquitination assay not included, H2A identity cannot be established (fig3b) yi_zhang@med.unc.edu 26-Sep-2011: Contacted by JYOTI. ORCHARD Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip fingerprinting peptide massfingerprinting fingerprinting Wang H., Wang L., Erdjument-Bromage H., Vidal M., Tempst P., Jones RS., Zhang Y. H2A ubiquitination assay not included, H2A identity cannot be established (fig3b) yi_zhang@med.unc.edu Accepted 2011-JUL-11 by ORCHARD 26-Sep-2011: Contacted by JYOTI. Nature (0028-0836) 2004 ORCHARD Only protein-protein interactions imex curation Role of histone H2A ubiquitination in Polycomb silencing. Role of histone H2A ubiquitination in Polycomb silencing. Nature (0028-0836) 2004 imex curation Wang H., Wang L., Erdjument-Bromage H., Vidal M., Tempst P., Jones RS., Zhang Y. H2A ubiquitination assay not included, H2A identity cannot be established (fig3b) yi_zhang@med.unc.edu 26-Sep-2011: Contacted by JYOTI. ORCHARD Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang H., Wang L., Erdjument-Bromage H., Vidal M., Tempst P., Jones RS., Zhang Y. H2A ubiquitination assay not included, H2A identity cannot be established (fig3b) yi_zhang@med.unc.edu Accepted 2011-JUL-11 by ORCHARD 26-Sep-2011: Contacted by JYOTI. Nature (0028-0836) 2004 ORCHARD Only protein-protein interactions imex curation ring1_human E3 ubiquitin-protein ligase RING1 RING finger protein 1 Polycomb complex protein RING1 RING1 RNF1 Really interesting new gene 1 protein RING-type E3 ubiquitin transferase RING1 protein protein human Homo sapiens Human MTTPANAQNASKTWELSLYELHRTPQEAIMDGTEIAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQAMHRAQRVRRPIPGSDQTTTMSGGEGEPGEGEGDGEDVSSDSAPDSAPGPAPKRPRGGGAGGSSVGTGGGGTGGVGGGAGSEDSGDRGGTLGGGTLGPPSPPGAPSPPEPGGEIELVFRPHPLLVEKGEYCQTRYVKTTGNATVDHLSKYLALRIALERRQQQEAGEPGGPGGGASDTGGPDGCGGEGGGAGGGDGPEEPALPSLEGVSEKQYTIYIAPGGGAFTTLNGSLTLELVNEKFWKVSRPLELCYAPTKDPK DIP protein Q06587 original sequence version: 103 DIP protein Q06587 original sequence version: 147 DIP protein Q06587 original sequence version: 135 6959787479DE9DAB ring2_human E3 ubiquitin-protein ligase RING2 RING finger protein 2 RING finger protein 1B RING finger protein BAP-1 Protein DinG Huntingtin-interacting protein 2-interacting protein 3 RNF2 BAP1 DING HIPI3 RING1B RING-type E3 ubiquitin transferase RING2 protein protein human Homo sapiens Human MSQAVQTNGTQPLSKTWELSLYELQRTPQEAITDGLEIVVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCADCIITALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSRDEYEAHQERVLARINKHNNQQALSHSIEEGLKIQAMNRLQRGKKQQIENGSGAEDNGDSSHCSNASTHSNQEAGPSNKRTKTSDDSGLELDNNNAAMAIDPVMDGASEIELVFRPHPTLMEKDDSAQTRYIKTSGNATVDHLSKYLAVRLALEELRSKGESNQMNLDTASEKQYTIYIATASGQFTVLNGSFSLELVSEKYWKVNKPMELYYAPTKEHK DIP protein Q99496 original sequence version: 95 DIP protein Q99496 original sequence version: 94 DIP protein Q99496 original sequence version: 96 DIP protein Q99496 original sequence version: 125 DIP protein Q99496 original sequence version: 142 DIP protein Q99496 original sequence version: 159 DIP protein Q99496 original sequence version: 129 DIP protein Q99496 original sequence version: 99 DIP protein Q99496 original sequence version: 118 90EA546E1F4DB7EC bmi1_human Polycomb complex protein BMI-1 BMI1 PCGF4 RNF51 Polycomb group RING finger protein 4 RING finger protein 51 protein protein human Homo sapiens Human MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETSKYCPICDVQVHKTRPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAAHPSADAANGSNEDRGEVADEDKRIITDDEIISLSIEFFDQNRLDRKVNKDKEKSKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELESDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSPSGNHQSSFANRPRKSSVNGSSATSSG DIP protein P35226 original sequence version: 105 DIP protein P35226 original sequence version: 136 DIP protein P35226 original sequence version: 110 DIP protein P35226 original sequence version: 129 030A7D396BADA543 phc2_human Polyhomeotic-like protein 2 Early development regulatory protein 2 PHC2 EDR2 PH2 protein protein human Homo sapiens Human MENELPVPHTSSSACATSSTSGASSSSGCNNSSSGGSGRPTGPQISVYSGIPDRQTVQVIQQALHRQPSTAAQYLQQMYAAQQQHLMLQTAALQQQHLSSAQLQSLAAVQQASLVSNRQGSTSGSNVSAQAPAQSSSINLAASPAAAQLLNRAQSVNSAAASGIAQQAVLLGNTSSPALTASQAQMYLRAQMLIFTPTATVATVQPELGTGSPARPPTPAQVQNLTLRTQQTPAAAASGPTPTQPVLPSLALKPTPGGSQPLPTPAQSRNTAQASPAGAKPGIADSVMEPHKKGDGNSSVPGSMEGRAGLSRTVPAVAAHPLIAPAYAQLQPHQLLPQPSSKHLQPQFVIQQQPQPQQQQPPPQQSRPVLQAEPHPQLASVSPSVALQPSSEAHAMPLGPVTPALPLQCPTANLHKPGGSQQCHPPTPDTGPQNGHPEGVPHTPQRRFQHTSAVILQLQPASPPQQCVPDDWKEVAPGEKSVPETRSGPSPHQQAIVTAMPGGLPVPTSPNIQPSPAHETGQGIVHALTDLSSPGMTSGNGNSASSIAGTAPQNGENKPPQAIVKPQILTHVIEGFVIQEGAEPFPVGRSSLLVGNLKKKYAQGFLPEKLPQQDHTTTTDSEMEEPYLQESKEEGAPLKLKCELCGRVDFAYKFKRSKRFCSMACAKRYNVGCTKRVGLFHSDRSKLQKAGAATHNRRRASKASLPPLTKDTKKQPTGTVPLSVTAALQLTHSQEDSSRCSDNSSYEEPLSPISASSSTSRRRQGQRDLELPDMHMRDLVGMGHHFLPSEPTKWNVEDVYEFIRSLPGCQEIAEEFRAQEIDGQALLLLKEDHLMSAMNIKLGPALKIYARISMLKDS DIP protein Q8IXK0 original sequence version: 67 2E3FD3898CFD702B ring1-rnf2-4 2 5 unspecified role unspecified role unspecified role unspecified role 6 unspecified role unspecified role unspecified role unspecified role physical association physical association 2c ring1-rnf2-1 1 5 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component physical association physical association 2b ring1-bmi1 3 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey association association 2d bmi1-ring1 4 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey association association 2e