IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3. A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3. J. Biol. Chem. (0021-9258) 2004 imex curation Simpson R.J., Yi Lee S.H., Bartle N., Sum E.Y., Visvader J.E., Matthews J.M., Mackay J.P., Crossley M. j.mackay@mmb.usyd.edu.au 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Simpson R.J., Yi Lee S.H., Bartle N., Sum E.Y., Visvader J.E., Matthews J.M., Mackay J.P., Crossley M. j.mackay@mmb.usyd.edu.au Accepted 2011-OCT-04 by MGT Murine erythroleukemia cell cDNA J. Biol. Chem. (0021-9258) 2004 Only protein-protein interactions imex curation A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3. A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3. J. Biol. Chem. (0021-9258) 2004 imex curation Simpson R.J., Yi Lee S.H., Bartle N., Sum E.Y., Visvader J.E., Matthews J.M., Mackay J.P., Crossley M. j.mackay@mmb.usyd.edu.au 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Simpson R.J., Yi Lee S.H., Bartle N., Sum E.Y., Visvader J.E., Matthews J.M., Mackay J.P., Crossley M. j.mackay@mmb.usyd.edu.au Accepted 2011-OCT-04 by MGT 21-Dec-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2004 Only protein-protein interactions imex curation A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3. A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3. J. Biol. Chem. (0021-9258) 2004 imex curation Simpson R.J., Yi Lee S.H., Bartle N., Sum E.Y., Visvader J.E., Matthews J.M., Mackay J.P., Crossley M. j.mackay@mmb.usyd.edu.au 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Simpson R.J., Yi Lee S.H., Bartle N., Sum E.Y., Visvader J.E., Matthews J.M., Mackay J.P., Crossley M. j.mackay@mmb.usyd.edu.au Accepted 2011-OCT-04 by MGT 21-Dec-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2004 Only protein-protein interactions imex curation A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3. A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3. J. Biol. Chem. (0021-9258) 2004 imex curation Simpson R.J., Yi Lee S.H., Bartle N., Sum E.Y., Visvader J.E., Matthews J.M., Mackay J.P., Crossley M. j.mackay@mmb.usyd.edu.au 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Simpson R.J., Yi Lee S.H., Bartle N., Sum E.Y., Visvader J.E., Matthews J.M., Mackay J.P., Crossley M. j.mackay@mmb.usyd.edu.au Accepted 2011-OCT-04 by MGT 21-Dec-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2004 Only protein-protein interactions imex curation A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3. A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3. J. Biol. Chem. (0021-9258) 2004 imex curation Simpson R.J., Yi Lee S.H., Bartle N., Sum E.Y., Visvader J.E., Matthews J.M., Mackay J.P., Crossley M. j.mackay@mmb.usyd.edu.au 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Simpson R.J., Yi Lee S.H., Bartle N., Sum E.Y., Visvader J.E., Matthews J.M., Mackay J.P., Crossley M. j.mackay@mmb.usyd.edu.au Accepted 2011-OCT-04 by MGT 21-Dec-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2004 Only protein-protein interactions imex curation A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3. A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3. J. Biol. Chem. (0021-9258) 2004 imex curation Simpson R.J., Yi Lee S.H., Bartle N., Sum E.Y., Visvader J.E., Matthews J.M., Mackay J.P., Crossley M. j.mackay@mmb.usyd.edu.au 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Simpson R.J., Yi Lee S.H., Bartle N., Sum E.Y., Visvader J.E., Matthews J.M., Mackay J.P., Crossley M. j.mackay@mmb.usyd.edu.au Accepted 2011-OCT-04 by MGT 21-Dec-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2004 Only protein-protein interactions imex curation A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3. A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3. J. Biol. Chem. (0021-9258) 2004 imex curation Simpson R.J., Yi Lee S.H., Bartle N., Sum E.Y., Visvader J.E., Matthews J.M., Mackay J.P., Crossley M. j.mackay@mmb.usyd.edu.au 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Simpson R.J., Yi Lee S.H., Bartle N., Sum E.Y., Visvader J.E., Matthews J.M., Mackay J.P., Crossley M. j.mackay@mmb.usyd.edu.au Accepted 2011-OCT-04 by MGT 21-Dec-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2004 Only protein-protein interactions imex curation tacc3_mouse Transforming acidic coiled-coil-containing protein 3 Tacc3 Aint ARNT-interacting protein protein protein mouse Mus musculus Mouse MSLHVLNDENVPNEKSSQCRDFQFLPPELTGRSSVLCLSQKENVPPQSQAKATNVTFQTPPRDPQTHRILSPNMTNKREAPFGLQNDHCVFLQKENQRPLAPVDDAPVVQMAAEILRAEGELQEGILTSSSLSASTSLLDSELVTPPIEPVLEPSHQGLEPVLESELVTPPVEPVLEPSHQELEPVLESELVTPPIEPVLEPSHQGLEPVLDSELVTPPIEPVLEPSHQGLEPVLESELVTPPIEPVLEPSHQGLEPVLDSELVTPPIEPVLEPSHQGLEPVLDSELVTPPIEPLLEPSHQGLEPVVDLKEESFRDPSEVLGTGAEVDYLEQFGTSSFKESAWRKQSLYVKFDPLLKDSPLRPMPVAPITNSTQDTEEESGSGKPTEAELVNLDFLGDLDVPVSAPTPVWSLEPRGLLPAEPIVDVLKYSQKDLDAVVNVMQQENLELKSKYEDLNTKYLEMGKSVDEFEKIAYKSLEEAEKQRELKEIAEDKIQKVLKERDQLNADLNSMEKSFSDLFKRFEKRKEVIEGYQKNEESLKKYVGECIVKIEKEGQRYQALKIHAEEKLRLANEEIAQVHSKAQAEVLALQASLRKAQMQNHSLEMTLEQKTKEIDELTRICDDLISKMEKI 92D6324D3890E9CB gata1_mouse Erythroid transcription factor Gata1 Gf-1 Eryf1 GATA-binding factor 1 NF-E1 DNA-binding protein protein protein mouse Mus musculus Mouse MDFPGLGALGTSEPLPQFVDSALVSSPSDSTGFFSSGPEGLDAASSSTSPNAATAAASALAYYREAEAYRHSPVFQVYPLLNSMEGIPGGSPYASWAYGKTALYPASTVCPSHEDAPSQALEDQEGKSNNTFLDTLKTERLSPDLLTLGTALPASLPVTGSAYGGADFPSPFFSPTGSPLSSAAYSSPKFHGSLPLAPCEARECVNCGATATPLWRRDRTGHYLCNACGLYHKMNGQNRPLIRPKKRMIVSKRAGTQCTNCQTTTTTLWRRNASGDPVCNACGLYFKLHQVNRPLTMRKDGIQTRNRKASGKGKKKRGSNLAGAGAAEGPAGGFMVVAGSSSSGNCGEVASGLALGTAGTAHLYQGLGPVVLSGPVSHLMPFPGPLLGSPTTSFPTGPAPTTSSTSVIAPLSS DIP protein P17679 original sequence version: 100 fog1_mouse Zinc finger protein ZFPM1 Zfpm1 Fog Fog1 Friend of GATA protein 1 Zinc finger protein multitype 1 protein protein mouse Mus musculus Mouse MSRRKQSNPRQIKRSLRDMEAGEEAKAMDSSPKEQEAPDPEAPAIEEPPSPPREDVSPPAVPAPPESPEDPEDMEGQELEMRPQDEEKEEKEEEAAMASPWSGPEELELALQDGQRCVRARLSLTEGLSWGPFYGSIQTRALSPEREEPGPAVTLMVDESCWLRMLPQVLTEEAANSEIYRKDDALWCRVTKVVPSGGLLYVRLVTEPHGAPRHPVQEPVEPGGLAPVHTDIQLLPQQAGMASILATAVINKDVFPCKDCGIWYRSERNLQAHLLYYCASRQRAGSPVSATEEKPKETYPNERVCPFPQCRKSCPSASSLEIHMRSHSGERPFVCLICLSAFTTKANCERHLKVHTDTLSGVCHNCGFISTTRDILYSHLVTNHMVCQPGSKGEIYSPGAGHPAAKLPPDSLAGFQQHSLMHSPLVPADKAPTPSSGLDSKAEVTNGETRVPPQNGGSSESPAAPRTIKVEAAEEPEATRASGPGEPGPQAPSRTPSPHSPNPVRVKTELSSPTPGSSPGPGELTMAGTLFLPQYVFSPDAGTTTVPTAPQASEILAKMSELVHNRLQQGAGSSGAAGTPTGLFSGTKGATCFECEITFNNINNFYVHKRLYCSGRRAPEDPPTVRRPKAATGPARAPAGAAAEPDPSRSSPGPGPREEEASGTTTPEAEAAGRGSEGSQSPGSSVDDAEDDPSRTLCEACNIRFSRHETYTVHKRYYCASRHDPPPRRPPAPTTAPGPAAPALTAPPVRTRRRRKLYELPAAGAPPPAAGPAPVPVVPSPTAELPSSPRPGSASAGPAPALSPSPVPDGPIDLSKRPRRQSPDAPTALPALADYHECTACRVSFHSLEAYLAHKKYSCPAAPLRTTALCPYCPPNGRVRGDLVEHLRQAHGLQVAKPAASPGAEPRTPAERAPRDSPDGRAPRSPSPAPENTPSDPADQGARTPSKGPPAPAPAPGGGGGHRYCRLCNIRFSSLSTFIAHKKYYCSSHAAEHVK DIP protein O35615 original sequence version: 82 293255B28151ECB8 zfpm1-tacc3 1 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15 13 physical association physical association zfpm1-gata1 1 9 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association zfpm1-tacc3-1 5 10 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 26 23 physical association physical association Fig. 1B tacc3-zfpm1 4 8 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role prey prey physical association physical association Fig. 1C zfpm1-tacc3-2 7 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role bait bait O35615:p.Thr343Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A Wed May 02 15:49:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr343ala' Tue Jun 30 17:45:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr343Ala' O35615:p.Thr344Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A Wed May 02 14:36:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr344ala' Tue Jun 30 16:43:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr344Ala' O35615:p.Asn347Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain N A Wed May 02 14:28:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn347ala' Tue Jun 30 19:35:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn347Ala' O35615:p.Arg350Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:08:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg350ala' Tue Jun 30 19:52:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg350Ala' O35615:p.Val354Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A Wed May 02 18:57:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val354ala' Tue Jun 30 19:46:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val354Ala' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 33 40 physical association physical association Fig. 1A,1D. tacc3-zfpm1-1 3 8 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O35615:p.[Glu330Lys;Leu336Arg;Glu349Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain E K certain certain sequence position certain certain certain sequence position certain L R certain certain sequence position certain certain certain sequence position certain E A Wed May 02 15:59:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu330lys,leu336arg,glu349ala' Tue Jun 30 17:21:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu330Lys;Leu336Arg;Glu349Ala]' region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 43 47 direct interaction direct interaction Fig. 2C zfpm1-tacc3-3 7 8 unspecified role unspecified role prey prey Q9JJ11:p.Arg619_Ile620delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain RI AA Thu Jul 07 12:16:03 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was arg619_ile620ala Fri Jul 22 11:11:55 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg619ala,ile620ala' Wed May 02 14:27:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_ile619-620ala_ala' Tue Jun 30 19:00:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg619_Ile620delinsAlaAla' Q9JJ11:p.Ile625_Ser626delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain IS AA Thu Jul 07 12:07:35 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was ile625_ser626ala Fri Jul 22 10:50:20 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ile625ala,ser626ala' Wed May 02 17:43:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile_ser625-626ala_ala' Tue Jun 30 18:36:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile625_Ser626delinsAlaAla' Q9JJ11:p.Glu629_Lys630delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain EK AA Thu Jul 07 12:07:35 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was glu629_lys630ala Fri Jul 22 10:53:19 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'glu629ala,lys630ala' Wed May 02 19:11:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_lys629-630ala_ala' Tue Jun 30 17:37:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu629_Lys630delinsAlaAla' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9JJ11:p.Asp615_Glu616delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain DE AA Thu Jul 07 12:15:59 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was asp615_glu616ala Fri Jul 22 11:06:57 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'asp615ala,glu616ala' Wed May 02 16:58:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_glu615-616ala_ala' Tue Jun 30 18:44:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp615_Glu616delinsAlaAla' Q9JJ11:p.Asp622_Asp623delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain DD AA Thu Jul 07 12:15:59 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was asp622-623ala Fri Jul 22 11:11:14 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'asp622ala,asp623ala' Wed May 02 15:12:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_asp622-623ala_ala' Tue Jun 30 16:39:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp622_Asp623delinsAlaAla' 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 55 59 physical association physical association Fig. 3 tacc3-tacc3 6 8 unspecified role unspecified role neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 4B tacc3-zfpm1-2 2 10 unspecified role unspecified role neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 67 70 direct interaction direct interaction Fig. 2D Ka=1.4 x 10E4 m-1 We do not have the unit terms to add Ka/Kon as a parameter.