UniProt UniProt http://www.uniprot.org EphB1 associates with Grb7 and regulates cell migration. EphB1 associates with Grb7 and regulates cell migration. J. Biol. Chem. (0021-9258) 2002 mimix curation Han D.C., Shen T.L., Miao H., Wang B., Guan J.L. jg19@cornell.edu 26-Sep-2011: Contacted by JYOTI. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Han D.C., Shen T.L., Miao H., Wang B., Guan J.L. jg19@cornell.edu Accepted 2011-JUL-15 by ORCHARD 26-Sep-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2002 orchard mimix curation EphB1 associates with Grb7 and regulates cell migration. EphB1 associates with Grb7 and regulates cell migration. J. Biol. Chem. (0021-9258) 2002 mimix curation Han D.C., Shen T.L., Miao H., Wang B., Guan J.L. jg19@cornell.edu 26-Sep-2011: Contacted by JYOTI. orchard crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. pull down pull down western blot western blot Immuno blot Immuno blot Han D.C., Shen T.L., Miao H., Wang B., Guan J.L. jg19@cornell.edu Accepted 2011-JUL-15 by ORCHARD 26-Sep-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2002 orchard mimix curation EphB1 associates with Grb7 and regulates cell migration. EphB1 associates with Grb7 and regulates cell migration. J. Biol. Chem. (0021-9258) 2002 mimix curation Han D.C., Shen T.L., Miao H., Wang B., Guan J.L. jg19@cornell.edu 26-Sep-2011: Contacted by JYOTI. orchard mouse-p19 Mus musculus embryonic teratocarcinoma cell line, epithelial-like, induced by retinoic acid p19 mouse embryonic teratocarcinoma cellline coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Han D.C., Shen T.L., Miao H., Wang B., Guan J.L. jg19@cornell.edu Accepted 2011-JUL-15 by ORCHARD 26-Sep-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2002 orchard mimix curation EphB1 associates with Grb7 and regulates cell migration. EphB1 associates with Grb7 and regulates cell migration. J. Biol. Chem. (0021-9258) 2002 mimix curation Han D.C., Shen T.L., Miao H., Wang B., Guan J.L. jg19@cornell.edu 26-Sep-2011: Contacted by JYOTI. orchard crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. pull down pull down anti tag western anti tag western blot anti tag western Han D.C., Shen T.L., Miao H., Wang B., Guan J.L. jg19@cornell.edu Accepted 2011-JUL-15 by ORCHARD 26-Sep-2011: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2002 orchard mimix curation EphB1 associates with Grb7 and regulates cell migration. EphB1 associates with Grb7 and regulates cell migration. J. Biol. Chem. (0021-9258) 2002 mimix curation Han D.C., Shen T.L., Miao H., Wang B., Guan J.L. jg19@cornell.edu 26-Sep-2011: Contacted by JYOTI. orchard yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Han D.C., Shen T.L., Miao H., Wang B., Guan J.L. jg19@cornell.edu Accepted 2011-JUL-15 by ORCHARD 26-Sep-2011: Contacted by JYOTI. HeLa cell library J. Biol. Chem. (0021-9258) 2002 orchard mimix curation grb7_human Growth factor receptor-bound protein 7 GRB7 GRB7 adapter protein Epidermal growth factor receptor GRB-7 B47 protein protein human Homo sapiens Human MELDLSPPHLSSSPEDLCPAPGTPPGTPRPPDTPLPEEVKRSQPLLIPTTGRKLREEERRATSLPSIPNPFPELCSPPSQSPILGGPSSARGLLPRDASRPHVVKVYSEDGACRSVEVAAGATARHVCEMLVQRAHALSDETWGLVECHPHLALERGLEDHESVVEVQAAWPVGGDSRFVFRKNFAKYELFKSSPHSLFPEKMVSSCLDAHTGISHEDLIQNFLNAGSFPEIQGFLQLRGSGRKLWKRFFCFLRRSGLYYSTKGTSKDPRHLQYVADVNESNVYVVTQGRKLYGMPTDFGFCVKPNKLRNGHKGLRIFCSEDEQSRTCWLAAFRLFKYGVQLYKNYQQAQSRHLHPSCLGSPPLRSASDNTLVAMDFSGHAGRVIENPREALSVALEEAQAWRKKTNHRLSLPMPASGTSLSAAIHRTQLWFHGRISREESQRLIGQQGLVDGLFLVRESQRNPQGFVLSLCHLQKVKHYLILPSEEEGRLYFSMDDGQTRFTDLLQLVEFHQLNRGILPCLLRHCCTRVAL A6867C20AFD46F74 ephb1_mouse Ephrin type-B receptor 1 Ephb1 protein protein mouse Mus musculus Mouse MALDCLLLFLLASAVAAMEETLMDTRTATAELGWTANPASGWEEVSGYDENLNTIRTYQVCNVFEPNQNNWLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTIAADESFSQVDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETMTGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGTFKASQEAEGCSHCPSNSRSPSEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGGRDDVTYNIICKKCRADRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAINGVSSKSPFPPQHVSVNITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNTARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTLTDDDYKSELREQLPLIAGSAAAGVVFVVSLVAISIVCSRKRAYSKEAAYSDKLQHYSTGRGSPGMKIYIDPFTYEDPNEAVREFAKEIDVSFVKIEEVIGAGEFGEVYKGRLKLPGKREIYVAIKTLKAGYSEKQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMIITEFMENGALDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLSEMNYVHRDLAARNILVNSNLVCKVSDFGLSRYLQDDTSDPTYTSSLGGKIPVRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPMDCPAALHQLMLDCWQKDRNSRPRFAEIVNTLDKMIRNPASLKTVATITAVPSQPLLDRSIPDFTAFTTVDDWLSAIKMVQYRDSFLTAGFTSLQLVTQMTSEDLLRIGVTLAGHQKKILSSIHSMRVQMNQSPSVMA E967019C82AA400A ephb1_human Ephrin type-B receptor 1 EPHB1 EPHT2 NET Tyrosine-protein kinase receptor EPH-2 HEK6 ELK EPH-like kinase 6 EPH tyrosine kinase 2 Neuronally-expressed EPH-related tyrosine kinase protein protein human Homo sapiens Human MALDYLLLLLLASAVAAMEETLMDTRTATAELGWTANPASGWEEVSGYDENLNTIRTYQVCNVFEPNQNNWLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTIAADESFSQVDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETMTGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGTFKASQEAEGCSHCPSNSRSPAEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGGRDDVTYNIICKKCRADRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAINGVSSKSPFPPQHVSVNITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNTARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTLTDDDYKSELREQLPLIAGSAAAGVVFVVSLVAISIVCSRKRAYSKEAVYSDKLQHYSTGRGSPGMKIYIDPFTYEDPNEAVREFAKEIDVSFVKIEEVIGAGEFGEVYKGRLKLPGKREIYVAIKTLKAGYSEKQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMIITEFMENGALDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLAEMNYVHRDLAARNILVNSNLVCKVSDFGLSRYLQDDTSDPTYTSSLGGKIPVRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPMDCPAALHQLMLDCWQKDRNSRPRFAEIVNTLDKMIRNPASLKTVATITAVPSQPLLDRSIPDFTAFTTVDDWLSAIKMVQYRDSFLTAGFTSLQLVTQMTSEDLLRIGITLAGHQKKILNSIHSMRVQISQSPTAMA 8044160E24E93A92 grb7-ephb1 5 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association fig1. grb7-ephb1 4 6 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association fig3. grb7-ephb1-3 2 6 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association fig2. figure 5 is a bit indirect evidence. They use Western blot probed with an antibody against phospho-Tyr, and the bands are at the expected position for Grb7 (pull-down) and its interaction partner EphB1 ephb1-ephb1 3 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association fig4 grb7-ephb1 1 6 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association fig1.