UniProt UniProt http://www.uniprot.org Establishment and maintenance of sister chromatid cohesion in fission yeast by a unique mechanism. Establishment and maintenance of sister chromatid cohesion in fission yeast by a unique mechanism. EMBO J. (0261-4189) 2001 imex curation Tanaka K., Hao Z., Kai M., Okayama H. okayama@m.u-tokyo.ac.jp 06-April-2011: Contacted by JYOTI. orchard Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Tanaka K., Hao Z., Kai M., Okayama H. Fig. 7B okayama@m.u-tokyo.ac.jp Accepted 2011-MAR-02 by ORCHARD 06-April-2011: Contacted by JYOTI. EMBO J. (0261-4189) 2001 orchard Only protein-protein interactions imex curation Establishment and maintenance of sister chromatid cohesion in fission yeast by a unique mechanism. Establishment and maintenance of sister chromatid cohesion in fission yeast by a unique mechanism. EMBO J. (0261-4189) 2001 imex curation Tanaka K., Hao Z., Kai M., Okayama H. okayama@m.u-tokyo.ac.jp 06-April-2011: Contacted by JYOTI. orchard Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Tanaka K., Hao Z., Kai M., Okayama H. okayama@m.u-tokyo.ac.jp Accepted 2011-MAR-02 by ORCHARD 06-April-2011: Contacted by JYOTI. EMBO J. (0261-4189) 2001 orchard Only protein-protein interactions imex curation Establishment and maintenance of sister chromatid cohesion in fission yeast by a unique mechanism. Establishment and maintenance of sister chromatid cohesion in fission yeast by a unique mechanism. EMBO J. (0261-4189) 2001 imex curation Tanaka K., Hao Z., Kai M., Okayama H. okayama@m.u-tokyo.ac.jp 06-April-2011: Contacted by JYOTI. orchard Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast fluorescence imaging fluorescence microscopy fluorescence imaging anti tag immunost anti tag immunostaining anti tag immunost Tanaka K., Hao Z., Kai M., Okayama H. okayama@m.u-tokyo.ac.jp Accepted 2011-MAR-02 by ORCHARD 06-April-2011: Contacted by JYOTI. EMBO J. (0261-4189) 2001 orchard Only protein-protein interactions imex curation rad21_schpo Cohesin subunit rad21 rad21 SPCC338.17c Double-strand-break repair protein rad21 SCC1 homolog protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MFYSEAILSKKGPLAKVWLAAHWEKKLSKVQTLHTSIEQSVHAIVTEETAPMALRLSGQLMLGVVRIYSRKARYLLEDCTEALMRLKMSFQPGQVDMIEPATALQSLKGKDAVTQSANLTLPETITEFDLLVPDSTFDFQWSQLLRTPSRSSNTLELHSLPISSSPSFPSSQLSIEAGRNAQVESGFSLGESFAHVGNDMQFHLPISNSGAATPRSVHSDNQSQISIEVGRDAPAAAATDLSGIIGPQMTKSPASSVTHFSTPSMLPIGGTSLDDELLAPVDDLNLDLGLDDLLGDEQGANAPAIEADEQAETSSIHLPSDIMEDDSSRPAAAGVEEGQVVESATAPQQEKINPQKTVRRQRAIIDPVTELSSKQMKKQLADTSSITSPLCLNTSSIVFNATVNFTRNGKFNTSIFSSNLNPKVNELLQADFKQAILRKRKNESPEEVEPAKHQRTDTSTENQETAEVLDPEEIAAAELANITEAAIATLPQETVVQPEGEAPELGSPMGFPVTALESADDSLFDAPPVMLDEADLLGSERLDSSVSEALPSSQTAKDSLRNKWDPYTEGEKVSFQTLSAGCNREEAVQLFFDVLVLATKDVISVKQDVAIQNEITLTAKRGMLLSSL DB60FF893AE0C9DE eso1_schpo N-acetyltransferase eso1 eso1 ECO1 homolog Sister chromatid cohesion protein eso1 SPBC16A3.11 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MELGKSKFSWKDLQYCDKAGTQNSPLRVVAHIDQDAFYAQVESVRLGLDHSVPLAVQQWQGLIAVNYAARAANISRHETVTEAKKKCPELCTAHVKTWKAGESEAKYHENPNPNYYKTCLDPYRHESVKILNIIKKHAPVVKKASIDECFIELTSDVKRIVLEEYPYLKIPSEDSNVALPQAPVLLWPAEFGMVIEEEVVDRTKEDYERDWDDVFLFYAAKIVKEIRDDIYLQLKYTCSAGVSFNPMLSKLVSSRNKPNKQTILTKNAIQDYLVSLKITDIRMLGGKFGEEIINLLGTDSIKDVWNMSMDFLIDKLGQTNGPLVWNLCHGIDNTEITTQVQIKSMLSAKNFSQQKVKSEEDAINWFQVFASDLRSRFLELEGMRRPKTICLTVVSRFLRKSRSSQIPMNVDISTQFIVEATSKLLRQLQQEFDVYPISNLSISFQNIIEVDRNSRGIEGFLKKSNDEIYMSTSVSPSIEGRAKLLNENMRENNSFELSSEKDIKSPKRLKRGKGKGIFDMLQQTAVSKPTENSADETYTCEECEQKITLSERNEHEDYHIALSISRKERYNNLVPPSHDKPKQVKPKTYGRKTGSKHYAPLSDETNNKRAFLDAFLGNGGNLTPNWKKQTPKAISNSSDNMTQLHLDLANSTVTCSECSMEYNSTSEEDILLHSRFHSRVLGGVTVSFQCSPIYRVNYGLSSDCIYSINSESSLIDQRKAEEALSFVNNELSSEPIETIGVDKYTTFLFISDKKCVGLLLAERISSAYIVDELELNNNNSTSSAVYIKNENLRKGFVLGISRIWVSASRRKQGIASLLLDNALKKFIYGYVISPAEVAFSQPSESGKQFIISWHRSRNNGSSKSLRYAVYES 36ECCD2BC0AEFE94 pds5_schpo Sister chromatid cohesion protein pds5 pds5 Precocious dissociation of sisters protein 5 SPAC110.02 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MIKLQFQRNIVPTHDNPLTTSEILKRLRDLLGELTSLSQDTIDRDSVLPVARSLVNNNLLHHKDKGIRSYTLCCIVELLRLCAPDAPFTLSQLEDIFQVILKILSGLMNQESTYYPQIYEILESLSNVKSAVLIVDLPNAEEFLVNIFRLFFDLARKGTTKNVEFYMLDIINQLINEINTIPAAALNILFAQLISGKGVRQTIGSSDSTNHGPAFQLARNIFHDSADRLQRYVCQYFSDIIFDSRDSLSDSMTTPEFIFSHNLVLQLWKYAPTTLLNIIPQFENELQAEQTSVRLVAIETVGLMLQDNAIWSDYPRVWSAFCGRLNDKSVACRIKCIEVASNALQNSLATSEIIENVVQMLQSKLADTDEKVRVATLKTIEQLTFETFKMQFSVQALKLMGDRLRDRKLNVRLQAIRTLSQIYNRAYQDLIDGVEYSIQMFSWIPSSLLEVFYVNDETTNAAVEICMAELVLQYLSSDTQTRLNRLFLSIKYFSEKAMRVFILLLQRQVKYSELLNYYIECCKNYNGGVMDNDEESITNKLKKVIDIISSKSSNPTLTEATFRKFAELNDRQSYKMLLQTFSIKSEYQVVLKSIKYLFKRVSETLSTASLECFRIFVYRSALFAFNKSNVHEIIQLLNEPVKYHNFLKPSEALLQHLPLIHPNIYGEVVIEVENIIVSSGIESDPKVIKALSQFSKRKKNFSIQTTTAEILRKLCLHGTQEQAKQAATIIAITETKEFKLDMITNIVENLEYNGGLPVRLMTLGQLFLYTLEEVEKVADQVTEFLVKKVIQRFPEKYDDTHNDEEWCTYEKLDNLTMCKVLAIRVLVNRLRAAAGGTEALNIGAPIIKLLKVLLMADGELSPFKNTPKISRAYLRLTASKYFLKLCSIPFYAEHIDFSSYVQISLLCQDENFDVRNLFLTKLQKQLQLKKLPISYYPLLFLTAVDPEEEIKTKASIWIRSQVAFFQKTHDFTMEYVATYLIHLLSHHPDISSIESENSLDFIAYIRFYVDTVVNSENVPIVFHLMQRIKQSYDVIEDGNNYIYVLSDMAQKILQVKSQNFGWSLTTYPKQIKLPYEILRPIPSIDEKKRIFNKIFITPKMESQIEHAIRTPVSSFAKQTTNKHANLKQKKTHSSKSDKKSSRRRKNEKRRKLNEQNPNIRNVPERSSSRFQGIRINYSEAPSSSEEISEEEEEISEEDFDEIEDL 935DABDE0A5E30FF pds5-rad21 3 4 unspecified role unspecified role neutral component neutral component c-ter ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role neutral component neutral component c-ter myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Fig. 4D eso1-pds5 1 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 7B pds5-eso1 1 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 7B rad21-pds5-1 2 6 unspecified role unspecified role prey prey c-ter myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role bait bait c-ter ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 4J