UniProt UniProt http://www.uniprot.org Yeast homologues of tomosyn and lethal giant larvae function in exocytosis and are associated with the plasma membrane SNARE, Sec9. Yeast homologues of tomosyn and lethal giant larvae function in exocytosis and are associated with the plasma membrane SNARE, Sec9. J. Cell Biol. (0021-9525) 1999 imex curation Lehman K., Rossi G., Adamo JE., Brennwald P. pjbrennw@mail.med.cornell.edu orchard Only protein-protein interactions in vitro In vitro pull down pull down autoradiography autoradiography Lehman K., Rossi G., Adamo JE., Brennwald P. pjbrennw@mail.med.cornell.edu Accepted 2011-APR-19 by ORCHARD J. Cell Biol. (0021-9525) 1999 orchard Only protein-protein interactions imex curation Yeast homologues of tomosyn and lethal giant larvae function in exocytosis and are associated with the plasma membrane SNARE, Sec9. Yeast homologues of tomosyn and lethal giant larvae function in exocytosis and are associated with the plasma membrane SNARE, Sec9. J. Cell Biol. (0021-9525) 1999 imex curation Lehman K., Rossi G., Adamo JE., Brennwald P. pjbrennw@mail.med.cornell.edu orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Lehman K., Rossi G., Adamo JE., Brennwald P. CoIP was performed in presence of a cross-linking agent DSP and with 35S radio-labeled proteins, and participants were revealed by a second step of IPs after cleaving the cross-link. pjbrennw@mail.med.cornell.edu Accepted 2011-APR-19 by ORCHARD J. Cell Biol. (0021-9525) 1999 orchard Only protein-protein interactions imex curation Yeast homologues of tomosyn and lethal giant larvae function in exocytosis and are associated with the plasma membrane SNARE, Sec9. Yeast homologues of tomosyn and lethal giant larvae function in exocytosis and are associated with the plasma membrane SNARE, Sec9. J. Cell Biol. (0021-9525) 1999 imex curation Lehman K., Rossi G., Adamo JE., Brennwald P. pjbrennw@mail.med.cornell.edu orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Lehman K., Rossi G., Adamo JE., Brennwald P. pjbrennw@mail.med.cornell.edu Accepted 2011-APR-19 by ORCHARD J. Cell Biol. (0021-9525) 1999 orchard Only protein-protein interactions imex curation sro77_yeast Lethal(2) giant larvae protein homolog SRO77 SRO77 SNI2 YBL106C YBL0806 SOP2 Suppressor of RHO3 protein 77 Sodium protection protein 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFKKSRHLKNVSNAIKSARVHDVSNGINSKFFDTKKICTYGINGRITVTTFDYTQSLLAVATTAGEIHVYGQKQIEVVFTLKNRPQIKHMRFIKGIYLIAVDEKSNIIVLSVHSKQILTTVFCPNSITCIETDPSLDWMLIGLESGSILIYDVDRNQMSKLKIENFQKSVFLPKERLSPVISIQWNPRDIGTILISYEHITVIYSFIDYKVKQHFFYQLEPYAPGGDLSTNIEKKRTPKVIQSLYHPNSLHILTVHEDNSLVFWDVNSGKLIHARSIFETHVNFPNPALKDCSFTETPAIFKVSWLCQRNPEYTSLLIATKATENPCLPQEITMIDLGGTPMYSVTSFDAMSKYYAKPVQQKLFSLIGKAPLINFLPLPKASPYFGGCHDTNLILLLLEDGELETLIYPAGSFSSKASIFPRSLAWVRPTVTTCIAQSVQKNLWLGMMTIAQSESFLKGGIPATRNIRRHETRSALLTGHSNGSVRIWDASHSEVTDNAVFEVNTAKVLNRATNLAIKNISFASETLELAVSSEVGDVILFKFETNKFYGQLPKSDALQLKFSRFSLDDSKTILVDVSDRGPTNVKQGFIPSTVIHAKKGAVSAIMNSNIGFVAVGFIEGTLIILDRRGPAIIFNENIRVISKAGSSYVSTVHFCVMEYGDDGFSSILMLCGTDIGELMTFKILPATNGRFEVKFTDATKTNNQGKILGINSFAKDTGYSCSATISKMQGLSKGIAIPGFVTISGANDIRLVSPGKSKDTHALFKYPIATSGLSFIPIIDGKGERKLSTIMIVLLINGDIKVLTVPELKEVKNLRCPVPLSAQYVENSSILENGDIVIRTGKFQASLISVLNESATGTNHTADISQHTPIDTLYNPDLKIGYRPQVNSLQWARGTIYCTPYQLDELLGGIERPESKYEESAIARGCISSSSSNAARKLPPGTEDHRYARPVRSSGRSNGYGVLKSVSRAIETRLDTVETTINDYATTMGQTMNDAMEETGRDMMKSAVGF 3547845334179A40 sro7_yeast Lethal(2) giant larvae protein homolog SRO7 SRO7 SNI1 YPR032W YP9367.12 SOP1 Suppressor of RHO3 protein 7 Polarity protein SRO7 Sodium protection protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFGSKRLKNVKEAFKSLKGQNSETPIENSKASFKSKNSKTSTISKDAKSSSSLKIPISSNNKNKIFSLAETNKYGMSSKPIAAAFDFTQNLLAIATVTGEVHIYGQQQVEVVIKLEDRSAIKEMRFVKGIYLVVINAKDTVYVLSLYSQKVLTTVFVPGKITSIDTDASLDWMLIGLQNGSMIVYDIDRDQLSSFKLDNLQKSSFFPAARLSPIVSIQWNPRDIGTVLISYEYVTLTYSLVENEIKQSFIYELPPFAPGGDFSEKTNEKRTPKVIQSLYHPNSLHIITIHEDNSLVFWDANSGHMIMARTVFETEINVPQPDYIRDSSTNAAKISKVYWMCENNPEYTSLLISHKSISRGDNQSLTMIDLGYTPRYSITSYEGMKNYYANPKQMKIFPLPTNVPIVNILPIPRQSPYFAGCHNPGLILLILGNGEIETMLYPSGIFTDKASLFPQNLSWLRPLATTSMAASVPNKLWLGALSAAQNKDYLLKGGVRTKRQKLPAEYGTAFITGHSNGSVRIYDASHGDIQDNASFEVNLSRTLNKAKELAVDKISFAAETLELAVSIETGDVVLFKYEVNQFYSVENRPESGDLEMNFRRFSLNNTNGVLVDVRDRAPTGVRQGFMPSTAVHANKGKTSAINNSNIGFVGIAYAAGSLMLIDRRGPAIIYMENIREISGAQSACVTCIEFVIMEYGDDGYSSILMVCGTDMGEVITYKILPASGGKFDVQLMDITNVTSKGPIHKIDAFSKETKSSCLATIPKMQNLSKGLCIPGIVLITGFDDIRLITLGKSKSTHKGFKYPLAATGLSYISTVEKNNDRKNLTVIITLEINGHLRVFTIPDFKEQMSEHIPFPIAAKYITESSVLRNGDIAIRVSEFQASLFSTVKEQDTLAPVSDTLYINGIRIPYRPQVNSLQWARGTVYCTPAQLNELLGGVNRPASKYKESIIAEGSFSERSSDDNNANHPEHQYTKPTRKGRNSSYGVLRNVSRAVETRWDAVEDRFNDYATAMGETMNEAVEQTGKDVMKGALGF FFB0901883009815 FFB0901883009815 sec9_yeast Protein transport protein SEC9 SEC9 HSS7 YGR009C protein protein yeast Saccharomyces cerevisiae Baker's yeast MGLKKFFKIKPPEEATPEQNKDTLMELGISVKNPSKKRKEKFAAYGKFANDKAEDKVYAPPGYEQYARPQDELEDLNASPLDANANEATAGSNRGSSGTQDLGNGAESNSMQDPYAIENDDYRYDDDPYARFQANKSNGRGSVNAAPYGDYGGGYNGTSLNSYNNDGPYSNQNTSNSWVNANGRNSLNHSNSTLNVGPSRQTRQPPVSTSTNSLSLDQRSPLANPMQEKRNPYADMNSYGGAYDSNTNRSSGTRQGSSKNANPYASMANDSYSNGNLNRSANPYSSRSVRQPQSQQAPMTYTPSFIASDEAARNSEVDLNEEPRTGEFDFEEVYADKSAENRAALDEPDLNAVMTNEDSIDLNASEVDHSSRQQQQQQWFMDEQQQQQQHFNATNNQYGDQRGYKTFEEIQKEEEARQQQEEDEAVDEIKQEIKFTKQSSVASTRNTLKMAQDAERAGMNTLGMLGHQSEQLNNVEGNLDLMKVQNKVADEKVAELKKLNRSILAVHVSNPFNSKRRRREREEQLKNRKIEEKLMREQTSQQLSQSTQRIEGAMNANNNISEVRERYQRKNVLEKAKRYQFENDEEDDEMELEIDRNLDQIQQVSNRLKKMALTTGKELDSQQKRLNNIEESTDDLDINLHMNTNRLAGIR EA314D73D20A10C7 EA314D73D20A10C7 sec9-sni1 3 6 unspecified role unspecified role bait bait c-terminal region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey c-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range certain certain sequence position certain physical association physical association No figure sec9-sni1-1 1 5 unspecified role unspecified role prey prey c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1A sec9-sni2 1 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Fig. 1A sec9-sni1-2 2 6 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 8