MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Importance of polyadenylation in the selective elimination of meiotic mRNAs in growing S. pombe cells. Importance of polyadenylation in the selective elimination of meiotic mRNAs in growing S. pombe cells. EMBO J. (0261-4189) 2010 imex curation Yamanaka S., Yamashita A., Harigaya Y., Iwata R., Yamamoto M. Only protein-protein interactions schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Yamanaka S., Yamashita A., Harigaya Y., Iwata R., Yamamoto M. EMBO J. (0261-4189) 2010 Only protein-protein interactions imex curation Importance of polyadenylation in the selective elimination of meiotic mRNAs in growing S. pombe cells. Importance of polyadenylation in the selective elimination of meiotic mRNAs in growing S. pombe cells. EMBO J. (0261-4189) 2010 imex curation Yamanaka S., Yamashita A., Harigaya Y., Iwata R., Yamamoto M. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Yamanaka S., Yamashita A., Harigaya Y., Iwata R., Yamamoto M. EMBO J. (0261-4189) 2010 Only protein-protein interactions imex curation Importance of polyadenylation in the selective elimination of meiotic mRNAs in growing S. pombe cells. Importance of polyadenylation in the selective elimination of meiotic mRNAs in growing S. pombe cells. EMBO J. (0261-4189) 2010 imex curation Yamanaka S., Yamashita A., Harigaya Y., Iwata R., Yamamoto M. Only protein-protein interactions schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Yamanaka S., Yamashita A., Harigaya Y., Iwata R., Yamamoto M. EMBO J. (0261-4189) 2010 Only protein-protein interactions imex curation Importance of polyadenylation in the selective elimination of meiotic mRNAs in growing S. pombe cells. Importance of polyadenylation in the selective elimination of meiotic mRNAs in growing S. pombe cells. EMBO J. (0261-4189) 2010 imex curation Yamanaka S., Yamashita A., Harigaya Y., Iwata R., Yamamoto M. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Yamanaka S., Yamashita A., Harigaya Y., Iwata R., Yamamoto M. EMBO J. (0261-4189) 2010 Only protein-protein interactions imex curation rrp6_schpo Exosome complex exonuclease rrp6 SPAC1F3.01 SPAC3H8.11 rrp6 Ribosomal RNA-processing protein 6 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDESELFKGLMNSTAYCSELAKVDIPFYKSIDTEFNENIKSVSSRFMRLIELLLSKVDRSRAEDIVDVEDIDNRWAEVSDTLDILFEKADYSIDKAQGLLKKPAIETHASTSDVANKKPKKEKLPYKVIHAAHLTKPQLRFRVQPNNSREFVWSWKLTEKPHSLVPLEKIIAQVKLDPSLKNSLPHPYEPEIQNSVYPPWVSEMSNPIDTGSVDETEPIWVSTESQLSDMLKELQNSKEIAVDLEHHDYRSFRGFVCLMQISNREKDWIVDTLELREELEALNVVFTNPNIIKVFHGATMDIIWLQRDFGLYVVNLFDTYYATKVLGFEGHGLAFLLQKYCDYDADKRYQMADWRIRPLPREMLKYAQSDTHYLLYIWDHLRNELISKSAERKENLMQSVFNSSKQISLRKYELEPYDPIYGLGTDGWRNVLTKFGSSKIIGREALMIYRALHDWRDSVARKEDESVRYVLPNRLLIAIAASKPVEAADVFSISKQLTPIARMYVEDIVKVVQEAEKLYNEQVDREKSQFKEVEKQNQPLAVFSESNTLGDYKVDSSVFEISKQNRSKLKTLLANGSAFWIEGQSQDDLRKARKERLFIVNQNIPFSLTLPCTQGHVESELNVKQSTVTEAANPSLNGEKKQEPIVIRDLGLNKQKRDSSKLNHKEPSNPIEERNEDIEPSEASTSVSKKRKQKKKKKNSGKLTIEAEHVSNDSPIINEAPFDYKNQKNFIADLDSDVGKNKFGKRGFNPLNKVSLPKRNTRELKKRKVSDGKSTSY mint DB1FAC172BFB14A2 mmi1_schpo RNA binding exosome specificity factor Mmi1 mmi1 Meiotic mRNA interception protein 1 YTH domain-containing protein mmi1 SPCC736.12c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSNTNFSTSRSSKSIPELPNLEALRSLWPPPSLNESGDTRSVWTTHTGEPVASSVLSTSGSNNFSSPLKRPAPESHDAPIGRRLMVDDPRLIKHGKYDFSRHCTDYGHSYEWPYFRSLRRESMLYHTSGSYPESQPPYSSYSTDAPHYYHAGSESSAYYDSRSRLHGIQPPPKRRTLSPPPRRLADPVVVGSSRYVEEEVYRRPPYTLASEVPSSASAYQAGYSSYPVRSSPQLSHEDTRHGIASSGSTRYPFVPANTRASHSPSLLEPYAHSLPSSVAPVGAYPEKSSYLLSNSSNDSASRKEKPKARASTPPPLNFSRASEHRNEKGERISMINPRVVLDENGISHRSRYFIMLCDNETAIAHAKKTSIWAVKKDSSKRISDAYKKASVYFIFVAQQTYNALGYAQVVSDLNSTELPFWSDSSHAGGVRIKWIKTCNLFSAEISEIVSHMDHGSEARDGMEMMYDEGSRLCTLINYAIMKRIGRDR mint 329704519DE3AE4A pap_schpo Poly(A) polymerase pla1 pla1 SPBC646.04 Polynucleotide adenylyltransferase protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MTTKQWGITPPISTAPATEQENALNTALINELKNQNLFESPAESEKRVKVLDELQQITTEFVKKVSLAKHMNEKMANEAGGKIFTYGSYRLGVYGPGSDIDTLVVVPKHVSRDNFFQDLEPMLREREEVTDLAAVPDAYVPIIKFKFLGISIDLIFARLSVPRVPRDLELSDNNLLKGVEERCVLSLNGTRVTDQILQLVPNRAVFKHALRAIKFWAQRRAIYANVVGFPGGVAWAMMVARICQLYPNAVSSVIVAKFFRILHQWNWPQPILLKPIEDGPLQVRIWNPKLYPSDKAHRMPIITPAYPSMCATHNITLSTQTIILREMVRAGEIADQIMVKALPWSALFQKHDFFHRYKHYLTITAAAKTAEAQLKWAGLVESKLRHLVTRLELVDAIALAHPFNKGFDKVYNCSSEEEAQQVASGVTLEVAYESTDHEKLANDTVNEEKADNTESKADGSENGEKQIFPVYTTTCYIGLELEKKKGHPIKRLDISWPTQEFYELCKKWDKYDDTLMNVFIKNTKNTALPDEVFEPGEERPKATKKRSTADTAHSTEQLKRQKVSTA mint Polymerase that creates the 3' poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor (CF I) E8E0E4165AAFD3D5 pab2_schpo Polyadenylate-binding protein 2 pab2 SPBC16E9.12c Poly(A)-binding protein II protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSDQDALDTQEKELLEMKERVAEMEAEAAKLRAMQEQLDNETEALRNDKESIDAQSVYVGNVDYSVTPEELQSHFASCGSVNRVTILCDKFTGHPKGFAYIEFSEPSLVPNALLLNGSMLHERPLKVTPKRTNVPGMSRGRGRGRGRGRGRGRGGYRGRARGFAPY mint A602B5EFC55600E8 dis3_schpo Exosome complex exonuclease dis3 dis3 SPBC26H8.10 rrp44 Chromosome disjunction protein 3 Ribosomal RNA-processing protein 44 Mitotic control protein dis3 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSTVSGLKRPQSSEKNHRDRVFVRATRGKVQKVVREQYLRNDIPCQSRACPLCRSKLPKDSRGNVLEPILSEKPMFLEKFGHHYLIPDSNIFYHCIDALEHPNNFFDVIILQTVFSEISSKSIPLYNRMKRLCQEKTKRFTPFSNEFFVDTFVERLDDESANDRNDRAIRNAASWFASHLASLGIKIVLLTDDRENARLAAEQGIQVSTLKDYVQYLPDSEILLDMVSAIADAIASKEQVESGTKNVYELHWSMSRLLACIKNGEVHKGLINISTYNYLEGSVVVPGYNKPVLVSGRENLNRAVQGDIVCIQILPQDQWKTEAEEIADDDEDVVVSTAAEPDSARINDLELITKRNAHPTAKVVGILKRNWRPYVGHVDNATIAQSKGGSQQTVLLTPMDRRVPKIRFRTRQAPRLVGRRIVVAIDLWDASSRYPEGHFVRDLGEMETKEAETEALLLEYDVQHRPFPKAVLDCLPEEGHNWKVPADKTHPLWKNRKDFRDKLICSIDPPGCQDIDDALHACVLPNGNYEVGVHIADVTHFVKPNTSMDSEAASRGTTVYLVDKRIDMLPMLLGTDLCSLRPYVERFAFSCIWEMDENANIIKVHFTKSVIASKEAFSYADAQARIDDQKMQDPLTQGMRVLLKLSKILKQKRMDEGALNLASPEVRIQTDNETSDPMDVEIKQLLETNSLVEEFMLLANISVAQKIYDAFPQTAVLRRHAAPPLTNFDSLQDILRVCKGMHLKCDTSKSLAKSLDECVDPKEPYFNTLLRILTTRCMLSAEYFCSGTFAPPDFRHYGLASPIYTHFTSPIRRYADVLAHRQLAAAIDYETINPSLSDKSRLIEICNGINYRHRMAQMAGRASIEYYVGQALKGGVAEEDAYVIKVFKNGFVVFIARFGLEGIVYTKSLSSVLEPNVEYVEDEYKLNIEIRDQPKPQTVQIQMFQQVRVRVTTVRDEHSGKQKVQITLVY mint Implicated in mitotic control. Essential for cell division and spore germination. May be involved in regulating protein dephosphorylation during mitosis 3802201244B18BAB mmi1-rrp6 3 rrp6 5 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined mmi1 6 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f5a mint mmi1-rrp6-1 3 rrp6 5 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined mmi1 6 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f5a mint dis3-mmi1 3 Dis3 9 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined mmi1 6 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f5b mint mmi1-pla1 3 mmi1 6 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Pla1 7 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f5c mint mmi1-pab2 3 mmi1 6 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Pab2 8 tag fluorescence tag visualisation by fluorescence tag fluorescence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f5d mint mmi1-pab2-1 1 Pab2 8 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell mmi1 6 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association sf3a mint mmi1-pla1-1 1 mmi1 6 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Pla1 7 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association sf3a mint mmi1-pab2-2 4 mmi1 6 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Pab2 8 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association sf3b mint mmi1-pla1-2 4 Pla1 7 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate mmi1 6 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association sf3b mint mmi1-pab2-3 2 Pab2 8 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined mmi1 6 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association sf1 mint