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  <entry>
    <source releaseDate="2022-02-04">
      <names>
        <shortLabel>MINT</shortLabel>
        <fullName>MINT, Dpt of Biology, University of Rome Tor Vergata</fullName>
        <alias type="synonym" typeAc="MI:1041">MINT</alias>
      </names>
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      </xref>
      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://mint.bio.uniroma2.it/mint</attribute>
        <attribute name="url" nameAc="MI:0614">url&amp;gt;http://mint.bio.uniroma2.it/mint/</attribute>
        <attribute name="url" nameAc="MI:0614">https://mint.bio.uniroma2.it</attribute>
        <attribute name="id-validation-regexp" nameAc="MI:0628">MINT-[0-9]+</attribute>
        <attribute name="search-url" nameAc="MI:0615">https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac}</attribute>
        <attribute name="definition">The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules.</attribute>
        <attribute name="url" nameAc="MI:0614">http://mint.bio.uniroma2.it</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Phosphatidylinositol-(4,5)-bisphosphate enables efficient secretion of HIV-1 Tat by infected T-cells.</fullName>
        </names>
        <bibref>
          <xref>
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            <secondaryRef db="imex" dbAc="MI:0670" id="IM-15074" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Phosphatidylinositol-(4,5)-bisphosphate enables efficient secretion of HIV-1 Tat by infected T-cells.</attribute>
            <attribute name="journal" nameAc="MI:0885">EMBO J. (0261-4189)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2010</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Rayne F., Debaisieux S., Yezid H., Lin YL., Mettling C., Konate K., Chazal N., Arold ST., Pugnière M., Sanchez F., Bonhoure A., Briant L., Loret E., Roy C., Beaumelle B.</attribute>
            <attribute name="imex-exported"/>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="mint" dbAc="MI:0471" id="MINT-7908951" refType="identity" refTypeAc="MI:0356"/>
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          <secondaryRef db="pubmed" dbAc="MI:0446" id="20224549" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="9606">
            <names>
              <shortLabel>human-t cells cd 4+</shortLabel>
              <fullName>Homo sapiens</fullName>
            </names>
            <tissue>
              <names>
                <shortLabel>t-cell_cd4+</shortLabel>
                <fullName>t cell cd4+</fullName>
              </names>
              <xref>
                <primaryRef db="intact" dbAc="MI:0469" id="IA:2473" refType="identity" refTypeAc="MI:0356"/>
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              </xref>
            </tissue>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>fluorescence imaging</shortLabel>
            <fullName>fluorescence microscopy</fullName>
            <alias type="synonym" typeAc="MI:1041">fluorescence imaging</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0416" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608494" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>immunostaining</shortLabel>
            <fullName>immunostaining</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0422" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608793" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Rayne F., Debaisieux S., Yezid H., Lin YL., Mettling C., Konate K., Chazal N., Arold ST., Pugnière M., Sanchez F., Bonhoure A., Briant L., Loret E., Roy C., Beaumelle B.</attribute>
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          <attribute name="publication year" nameAc="MI:0886">2010</attribute>
          <attribute name="imex-exported"/>
          <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
          <attribute name="imex curation" nameAc="MI:0959"/>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>gag_hv1br</shortLabel>
          <fullName>Gag polyprotein</fullName>
          <alias type="gene name" typeAc="MI:0301">gag</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Pr55Gag</alias>
        </names>
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          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-7845043" refType="identity" refTypeAc="MI:0356"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005198" version="SP_160"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0008270" version="SP_160"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0016020" version="SP_160"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0019013" version="SP_160"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0020002" version="SP_160"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0042025" version="SP_160"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0055036" version="SP_160"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0072494" version="SP_160"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0039702" version="SP_160"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR012344"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR008919"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR014817"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR000721"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR000071"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR008916"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR001878"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR010999"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR036875"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
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            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="11686">
          <names>
            <shortLabel>hv1br</shortLabel>
            <fullName>Human immunodeficiency virus type 1 group M subtype B (isolate BRU/LAI)</fullName>
            <alias type="synonym" typeAc="MI:1041">HIV-1</alias>
          </names>
        </organism>
        <sequence>MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFAVNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKIEEEQNKSKKKAQQAAADTGHSSQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQGVGGPGHKARVLAEAMSQVTNSATIMMQRGNFRNQRKIVKCFNCGKEGHIARNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSYKGRPGNFLQSRPEPTAPPFLQSRPEPTAPPEESFRSGVETTTPSQKQEPIDKELYPLTSLRSLFGNDPSSQ</sequence>
        <attributeList>
          <attribute name="comment" nameAc="MI:0612">mint</attribute>
          <attribute name="crc64">9103D701A843466F</attribute>
        </attributeList>
      </interactor>
      <interactor id="3">
        <names>
          <shortLabel>tat_hv1br</shortLabel>
          <fullName>Protein Tat</fullName>
          <alias type="gene name" typeAc="MI:0301">tat</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Transactivating regulatory protein</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P04610" version="SP_84" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="MINT-7908963" refType="intact-secondary"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005576" version="SP_151"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0006351" version="SP_151"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0010801" version="SP_151"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0032968" version="SP_151"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0042805" version="SP_151"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0044196" version="SP_151"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0046872" version="SP_151"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0050434" version="SP_151"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0051092" version="SP_151"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0052312" version="SP_151"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0060081" version="SP_151"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:1900182" version="SP_151"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:1901856" version="SP_151"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:1990970" version="SP_151"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1JFW"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1JM4"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR001831"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR036963"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
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            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="11686">
          <names>
            <shortLabel>hv1br</shortLabel>
            <fullName>Human immunodeficiency virus type 1 group M subtype B (isolate BRU/LAI)</fullName>
            <alias type="synonym" typeAc="MI:1041">HIV-1</alias>
          </names>
        </organism>
        <sequence>MEPVDPRLEPWKHPGSQPKTACTTCYCKKCCFHCQVCFTTKALGISYGRKKRRQRRRPPQGSQTHQVSLSKQPTSQPRGDPTGPKE</sequence>
        <attributeList>
          <attribute name="comment" nameAc="MI:0612">mint</attribute>
          <attribute name="comment" nameAc="MI:0612">homomint</attribute>
          <attribute name="crc64">9B1B4A915FAF8A14</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4" imexId="IM-15074-1">
        <names>
          <shortLabel>gag-tat</shortLabel>
        </names>
        <xref>
          <primaryRef db="mint" dbAc="MI:0471" id="MINT-7909043" refType="identity" refTypeAc="MI:0356"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005886" refType="go-definition-ref" refTypeAc="MI:0242"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-15074-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
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        <participantList>
          <participant id="5">
            <names>
              <alias type="author assigned name" typeAc="MI:0345">p24</alias>
            </names>
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            </xref>
            <interactorRef>2</interactorRef>
            <participantIdentificationMethodList>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>immunostaining</shortLabel>
                  <fullName>immunostaining</fullName>
                </names>
                <xref>
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              </participantIdentificationMethod>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>predetermined</shortLabel>
                  <fullName>predetermined participant</fullName>
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                </xref>
              </participantIdentificationMethod>
            </participantIdentificationMethodList>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
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                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
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                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>over-expressed</shortLabel>
                  <fullName>over expressed level</fullName>
                  <alias type="synonym" typeAc="MI:1041">over-expressed</alias>
                </names>
                <xref>
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                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
              <experimentalPreparation>
                <names>
                  <shortLabel>fixed cell</shortLabel>
                  <fullName>fixed cell</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0348" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537839" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <featureList>
              <feature id="6">
                <names>
                  <shortLabel>p24</shortLabel>
                  <fullName>p24</fullName>
                </names>
                <xref>
                  <primaryRef db="mint" dbAc="MI:0471" id="MINT-7909053" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-7845097" refType="identity" refTypeAc="MI:0356"/>
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                <featureType>
                  <names>
                    <shortLabel>polyprotein frag</shortLabel>
                    <fullName>polyprotein fragment</fullName>
                    <alias type="synonym" typeAc="MI:1041">polyprotein frag</alias>
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                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0828" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1218736" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14577292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
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                      </xref>
                    </startStatus>
                    <begin position="133"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
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                    </endStatus>
                    <end position="363"/>
                  </featureRange>
                </featureRangeList>
              </feature>
            </featureList>
          </participant>
          <participant id="7">
            <names>
              <alias type="author assigned name" typeAc="MI:0345">TAT</alias>
            </names>
            <xref>
              <primaryRef db="mint" dbAc="MI:0471" id="MINT-7909045" refType="identity" refTypeAc="MI:0356"/>
            </xref>
            <interactorRef>3</interactorRef>
            <participantIdentificationMethodList>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>immunostaining</shortLabel>
                  <fullName>immunostaining</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0422" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608793" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </participantIdentificationMethod>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>predetermined</shortLabel>
                  <fullName>predetermined participant</fullName>
                  <alias type="synonym" typeAc="MI:1041">predetermined</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </participantIdentificationMethod>
            </participantIdentificationMethodList>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>over-expressed</shortLabel>
                  <fullName>over expressed level</fullName>
                  <alias type="synonym" typeAc="MI:1041">over-expressed</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0506" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537736" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
              <experimentalPreparation>
                <names>
                  <shortLabel>fixed cell</shortLabel>
                  <fullName>fixed cell</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0348" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537839" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>colocalization</shortLabel>
            <fullName>colocalization</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0403" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608832" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="comment" nameAc="MI:0612">homomint</attribute>
          <attribute name="comment" nameAc="MI:0612">mint</attribute>
          <attribute name="source-text">Neomycin displaces Tat and Gag from the plasma membrane of infected cells.</attribute>
          <attribute name="figure legend" nameAc="MI:0599">f2d sf1 f5d</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>