MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Bactofilins, a ubiquitous class of cytoskeletal proteins mediating polar localization of a cell wall synthase in Caulobacter crescentus. Bactofilins, a ubiquitous class of cytoskeletal proteins mediating polar localization of a cell wall synthase in Caulobacter crescentus. EMBO J. (0261-4189) 2010 imex curation Kühn J., Briegel A., Mörschel E., Kahnt J., Leser K., Wick S., Jensen GJ., Thanbichler M. Only protein-protein interactions strain na1000 / cb15 Caulobacter crescentus solution sedimentati cosedimentation in solution solution sedimentati western blot western blot Immuno blot Immuno blot Kühn J., Briegel A., Mörschel E., Kahnt J., Leser K., Wick S., Jensen GJ., Thanbichler M. EMBO J. (0261-4189) 2010 Only protein-protein interactions imex curation Bactofilins, a ubiquitous class of cytoskeletal proteins mediating polar localization of a cell wall synthase in Caulobacter crescentus. Bactofilins, a ubiquitous class of cytoskeletal proteins mediating polar localization of a cell wall synthase in Caulobacter crescentus. EMBO J. (0261-4189) 2010 imex curation Kühn J., Briegel A., Mörschel E., Kahnt J., Leser K., Wick S., Jensen GJ., Thanbichler M. Only protein-protein interactions caucr Caulobacter crescentus (strain ATCC 19089 / CB15) Caulobacter vibrioides fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Kühn J., Briegel A., Mörschel E., Kahnt J., Leser K., Wick S., Jensen GJ., Thanbichler M. EMBO J. (0261-4189) 2010 Only protein-protein interactions imex curation Bactofilins, a ubiquitous class of cytoskeletal proteins mediating polar localization of a cell wall synthase in Caulobacter crescentus. Bactofilins, a ubiquitous class of cytoskeletal proteins mediating polar localization of a cell wall synthase in Caulobacter crescentus. EMBO J. (0261-4189) 2010 imex curation Kühn J., Briegel A., Mörschel E., Kahnt J., Leser K., Wick S., Jensen GJ., Thanbichler M. Only protein-protein interactions caucr Caulobacter crescentus (strain ATCC 19089 / CB15) Caulobacter vibrioides anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Kühn J., Briegel A., Mörschel E., Kahnt J., Leser K., Wick S., Jensen GJ., Thanbichler M. EMBO J. (0261-4189) 2010 Only protein-protein interactions imex curation q9a753_cauvc bacA CC_1873 protein protein caucr Caulobacter crescentus (strain ATCC 19089 / CB15) Caulobacter vibrioides MFSKQAKSNNKAPARIEPLPTPMAATPAEPARRAPPKVASLLSADLTIEGGVTGEGELQIDGVVKGDVRVGRLTVGETGHVEGSVYAEAVEVRGRVVGAITSKQVRLYGTSYVDGDITHEQLAMETGAFFQGRSLKFQRPAPAPSQPAPHPEHLAIAKSAG mint 2573B3B3A16C127E a0a0h3c9y4_cauvn Lysozyme spmX CCNA_02255 protein protein strain na1000 / cb15 Caulobacter crescentus MKPRHQVSRAAVDLIKRFEGYRQKAAQLPDGRWTVGYGHTLTAREGASVSEKDAEALLLYDLISVAHSVNEHTYTPLNQNQFDALVCFAFNIGLDNFLRSGVLRRINEGSLLQAACAMEMWRKADFEGERIVIDALVRRRSAEKTLFLTPANGEWVPAPSPVLRPKVDYDASCAVPKQTPATVNTRTEGDRVIAQREEGLPLNVVVPEEDAPTATEQSAAAVAARLEAILPETPSAPASMAKPAMRQDDLGLPEPPAPTQPAAMEPAPVLFEAEPQPAPVEPPPAAPVEFTPFRLTPQASEQAEAPVEAPAPTRPAPSEPTLFGAPATGSSVFNLDGFSTSEDATVGVMDVPLVEPEPPRFGNTSLLISLGILGLALFGGGVLWILTVAAGSDGQVKLFGYGASMVGAICFVVSAYLLLRRLAGPDVSDEE mint 0BBD75D596B3250E q9a3c7_cauvc Penicillin-insensitive transglycosylase Peptidoglycan TGase CC_3277 protein protein caucr Caulobacter crescentus (strain ATCC 19089 / CB15) Caulobacter vibrioides MNDWTLPPYKFDDGKSPGEPPKPGPASASDGVSQDPWAPQGPDPFRLSSDAATPPPPPEPPPEEPFRADLKHAAAKKKARKWGWVWGTLLVGFLLAVLSVAGGGAYVWFKYLKDTPALPSREALFAVNRAPGIRFEDRNGQVIATRGPRYGQRITLGTVPNYVPMAFLAAEDRRFYQHGAIDVQGIARAAWINWRAGKTRQGASTLTQQLAKGLFLTPDRVVKRKLQEMLLAYKLEQILTKDEILELYLNRIYFGAGTYGIDGASQTYFGKPASQLTLSEAALLASLPKAPSRLALTRDMERALARSRLILANMRKEGWITPEQESRALDDTPRLSPMALQDEGDYGWVLDYATAEAVKIAGQNAPDLVVRLTIDPLLQSEGAEVVRQTMATETTRSGASQAALLSLSADGAIRAMVGGTDYSESPFNRAVQAKRQPGSTFKPFVYAAAVEKGVLPTDMRVDEPVKFGTWEPENYSGGYRGPMTVEDALVTSINTVAVKLGQEVGGPAIGDLVRRFGITSLPPSPDLSVALGSYEVNLLQITSGFQVFQQGGLRIEPYVIESITTQGGQQIFQHQPPQGERRVYDVAHASMMVKMMKKVVTQGTAQRAAFGRPVAGKTGTSQNWRDAWFVGFTPDYVTGVWVGNDDEKPMNKVVGGDIPASIWRRFMMTAHQTLAVRDFEWLLPDPAPQSEPDPRNGFYETLSAEFSRAASELETTTPVAPAPGQPPPDNLPY mint D7F31A03BF19C7BF q9a421_cauvc CC_3022 protein protein caucr Caulobacter crescentus (strain ATCC 19089 / CB15) Caulobacter vibrioides MSSLKLPADNPTFLAMVGSCQVWSPGLDNAGKRPRSCCLGLPNAHFETRSPPMFAKKKETPAKLALTPLEPQRAATPQVAQPQPQPAPAPARPKPASLIAQGLTIRGDVVGDIEVHLDCVVMGDVKVGKLIVGPNARIEGAVVAQAMEIHGMVIGAVTAQSVRLYGTARVDGDITHEQLAMEPGAEFQGRSLKFRRPAPTPQPGQQAPAAPTPAAPPTPPSASQTPYNHSAH mint BF6DE256CA4F4358 baca-q9a421 1 BacB 7 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level BacA 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level SpmX 5 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization f2b mint baca-q9a421-1 2 BacB 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined BacA 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell cfp tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f1b mint baca-q9a421-2 2 BacB 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined BacA 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell cfp tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f4b mint baca-q9a421-3 3 BacB 7 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level BacA 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f4a mint q9a421-baca 3 BacA 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level BacB 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f4a mint baca-q9a3c7 3 BacA 4 weight silver stain molecular weight estimation by silver staining weight silver stain predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PbpC 6 ms participant Identification by mass spectrometry ms participant predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level BacB 7 ms participant Identification by mass spectrometry ms participant predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association f5a mint q9a421-baca-1 3 PbpC 6 ms participant Identification by mass spectrometry ms participant predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level BacB 7 weight silver stain molecular weight estimation by silver staining weight silver stain predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined BacA 4 ms participant Identification by mass spectrometry ms participant predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association f5a mint baca-q9a3c7-1 3 BacA 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell cfp tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PbpC 6 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization mint f5c f5d Finally, overproduction of a BacA eCFP fusion caused Venus PbpC to adopt an elongated localization pattern identical to that observed for BacA eCFP (Figure 5D).