IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Galpha4 G protein subunit interacts with the MAP kinase ERK2 using a D-motif that regulates developmental morphogenesis in Dictyostelium. The Galpha4 G protein subunit interacts with the MAP kinase ERK2 using a D-motif that regulates developmental morphogenesis in Dictyostelium. Dev. Biol. (0012-1606) 2009 imex curation Nguyen HN., Hadwiger JA. jeff.hadwiger@okstate.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions dicdi Dictyostelium discoideum (Slime mold) Slime mold anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Nguyen HN., Hadwiger JA. jeff.hadwiger@okstate.edu Accepted 2010-OCT-3 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Dev. Biol. (0012-1606) 2009 jyoti Only protein-protein interactions imex curation The Galpha4 G protein subunit interacts with the MAP kinase ERK2 using a D-motif that regulates developmental morphogenesis in Dictyostelium. The Galpha4 G protein subunit interacts with the MAP kinase ERK2 using a D-motif that regulates developmental morphogenesis in Dictyostelium. Dev. Biol. (0012-1606) 2009 imex curation Nguyen HN., Hadwiger JA. jeff.hadwiger@okstate.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions dicdi Dictyostelium discoideum (Slime mold) Slime mold pull down pull down anti tag western anti tag western blot anti tag western Nguyen HN., Hadwiger JA. jeff.hadwiger@okstate.edu Accepted 2010-OCT-3 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Dev. Biol. (0012-1606) 2009 jyoti Only protein-protein interactions imex curation erk2_dicdi Extracellular signal-regulated kinase 2 erkB dagC erk2 Defective in aggregation protein C MAP kinase 2 DDB_G0283903 protein protein dicdi Dictyostelium discoideum (Slime mold) Slime mold MSSEDIDKHVLRKYEVLQKIGKGAYGIVWKAIDKKTKQTVALKKIFDAFQNATDAQRTFREIMFLQELHGHENIIKLLNVIKADNDRDIYLVFEHMETDLHAVIRAKILEEIHKQYTIYQLLKALKYMHSANVLHRDIKPSNLLLNSECLVKVADFGLARSITSLESIAEANPVLTEYVATRWYRAPEILLGSTKYTKGVDMWSIGCILGELLGEKAMFPGNSTMNQLDLIIEVTGRPSAEDIEAIKSPFAGTMLESLPPSNPRSLSDMYPSASVDALDLLKKLLQFNPDKRITAEEALAHPFVTQFHNPAEEPHFDRIIKISIDDGQKFPIAEYRNRLYNDIIKKKKEERKKQTNPTKPDTTAPTLST 3AB5C85E6F10722B gpa4_dicdi Guanine nucleotide-binding protein alpha-4 subunit gpaD DDB_G0285425 protein protein dicdi Dictyostelium discoideum (Slime mold) Slime mold MRFKCFGSEETEQSSKIDKSIETDRRKLRKDVKLLLLGPGESGKSTIFKQMKIIQEDGGYSVEELLEYRAFVYSNCISQMEALLTASAKLNIELEVENKQRAANVLRRTIGNEPWLLLAADIKHLWEDKGIKETYAQKDKHFQLNDSAAYFFDNIDRYMREDFVPNEQDVLRCRVRTTGIQESEFTFDKIRLKIVDVGGQRSQRRKWIHCFDCVTAVIFVAAMSDYDQVLREDESVNRTRESLALFKEIVNCDYFKETPIVLFLNKKDLFKEKLKRVPLQSCFSDYTGPNKYKDAAMFIQSQYLAQGPSPRTIYTHATCAVDTENIKFVFRAVRQTILSQALEHF 6B92FF0FFF5775B9 erk2-gpad 2 4 unspecified role unspecified role prey prey P34042:p.Arg107_Arg108delinsGluGlu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain RR EE Thu Jul 07 12:17:29 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was arg107_108glu Fri Jul 22 11:14:38 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg107glu,arg108glu' Wed May 02 18:01:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg107-108glu_glu' Tue Jun 30 19:04:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg107_Arg108delinsGluGlu' P34042:p.Gln200Leu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 15:50:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln200leu' Tue Jun 30 19:35:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln200Leu' undefined mutation arg107_108leu gln200leu mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:00:17 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'mutated region' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Possible Gα4 subunit-ERK2 interactions in Dictyostelium were investigated using a pull-down assay that examined the presence of a Myc-tagged Gα4 subunit in the extraction of a His6-tagged ERK2 protein. Fig. 2D, Fig 3 erk2-gpad-1 1 4 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association The Gα4 subunit-ERK2 interaction was also demonstrated by the detection of the His6-ERK2 in immunoprecipitates of myc-Gα4 subunit. In erk2− cells transformed with the His6-ERK2 and myc-Gα4 subunit expression vectors, the Myc-Gα4 subunits were immunoprecipitated using α-Myc antibodies and the resulting immune complexes contained the His6-ERK2 Fig. 2E