MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it A longevity protein, Lag2, interacts with SCF complex and regulates SCF function. A longevity protein, Lag2, interacts with SCF complex and regulates SCF function. EMBO J. (0261-4189) 2009 imex curation Liu Y., Mimura S., Kishi T., Kamura T. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Liu Y., Mimura S., Kishi T., Kamura T. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation A longevity protein, Lag2, interacts with SCF complex and regulates SCF function. A longevity protein, Lag2, interacts with SCF complex and regulates SCF function. EMBO J. (0261-4189) 2009 imex curation Liu Y., Mimura S., Kishi T., Kamura T. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Liu Y., Mimura S., Kishi T., Kamura T. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation A longevity protein, Lag2, interacts with SCF complex and regulates SCF function. A longevity protein, Lag2, interacts with SCF complex and regulates SCF function. EMBO J. (0261-4189) 2009 imex curation Liu Y., Mimura S., Kishi T., Kamura T. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Liu Y., Mimura S., Kishi T., Kamura T. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation skp1_yeast Suppressor of kinetochore protein 1 SKP1 CBF3D YDR328C D9798.14 Centromere DNA-binding protein complex CBF3 subunit D E3 ubiquitin ligase complex SCF subunit SKP1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVTSNVVLVSGEGERFTVDKKIAERSLLLKNYLNDMHDSNLQNNSDSESDSDSETNHKSKDNNNGDDDDEDDDEIVMPVPNVRSSVLQKVIEWAEHHRDSNFPDEDDDDSRKSAPVDSWDREFLKVDQEMLYEIILAANYLNIKPLLDAGCKVVAEMIRGRSPEEIRRTFNIVNDFTPEEEAAIRRENEWAEDR 746DDE6470A69432 746DDE6470A69432 saf1_yeast SCF-associated factor 1 YBR2017 YBR280C SAF1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEVESREKEPDAGLSPDIVQATLPFLSSDDIKNLSQTNKYYNTLLDFDHSKILWHELFHKAFGTLKTNDEPFQGRNSAEFKTCTETILREAFPTLSWQEVYQLRAYDAKFYSWGYLKHGRLGYTASSNNELVATSLNGPSPRFKYGVNTPTEVPWFNSRTTSRTSNFTPSEDPLSAIKKDGDEIIAQVSSGGFSFQILTESGNLYSSGSTFSGGLKGPGPSGSQHDYNPFREMIHNMERSYPRITSRSNGSTVNTTGTFSGRRMSGSHPSTAYEPGNATTAQHITIDSGGAPAASPGGSHSGVPRTTMPSMGPHENIYSQIEMLERSANKAVPGNNHIRRMFARNSFPLYSGRDENLGSFNDIQFVAVSSGRSHFLAMDTDNNIYSWDSTESDQGVKIEFANLPSRATNPILKIASGWNFNCCYIYKVGLVAWKERDAIQKGESFAFAKYEIVPNTNDVNGDSRIVDFACLQDNCVFFINNNGDKLWKYHNGLNQIVDLNIVGKLCKINACFASLVLFTDTHCYTLKVTNGDVDKDSLTELDINENVISVASGDYHTVALTERGHLYSWGIESQDCGCLGLGPSEKIVNELHIGNWEGQRNIRVVKPTKIELPEDYICVSVTAGGWQTGALIIKKH 358D751C612744BE 358D751C612744BE aim23_yeast Altered inheritance of mitochondria protein 23, mitochondrial YJL131C J0682 AIM23 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLKVPLSDVLSQKMLFLKSFRYFHCTKYFSRDNASSTTDIFRNAMKRKRELANLKEQSHGNVARNAAFPKEYIKRPKQVPRNATNRKKILITWSTGTDRAKEAANSVVSEIFKKNHKGNIKVVDPTTHRIEASNIRYFAKGIDLDKVGLSIVNVEQIDNENQIPLVKIVESRVALKKYSDFLAKKKEKELMELGVLNKSYKNLVTDKKEDNLKHIKISWQIESDDLKRQKAHEIVSLLKKGNKVTLYLDDKNNINSNNWLENFEELDRSQKGEPPRLPESVFQKRAAVLETLKEIVSEYANDPVLLGNMNSKMIMKLIPKDVKPQNNDKRALKELRKKERQEKLQKRIQRKKMNEM BCF907223417B6C2 lag2_yeast Cullin-associated NEDD8-dissociated protein 1 homolog LAG2 CAND1 homolog Longevity-assurance protein 2 YOL025W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLHISKLIEQYRSTKDNDLKYMLLRQNFKINDIEDELAPLVNELLLPVLVEEQDMEILNLVSFQVLPDLVLSMISDPAAAQLGWVISLICDPLLNQSMIHANRSFVLIETLRNVLQKIENSPHLDYHQPVNSSLEFISKFIVEMKRHMCDVDAAQLSHSLSESNMLIYIESLNLLLKFSFFSDAASPSVMVTLPFDILNDVFTIAQDYSATNTNESIDRITEKLLLTSTQLTHPVDLENLCPKMKYNTLAAVSRIWYKFGPIVDKLFTNRLLPVLFPPQMGEECNVEDVLEIVHNFHPYFSIRRLKDNRPLLSDSTISQLREGLFGMLSILNDSLTRTQNENDHGSDNLIDSDDGFGSDNDPEQQAYLDELVSEGYDENMYDGDTDDEDADDINVEKNDEATKDITETNKILLIFSELHYPQEERFSELLVELQTKIAINTSLIDKILSKETTELPTHNGEIADLNEILNEVKGNKPIRKNVIFCTLAHTLSLQSGSELSVLQLSIEVIDHLLVKNHSNNITRGEQFQLIKLILPHLKTNKSFIDTLKAGNFTQKIDEGVTLRTMILSLLLQLFPLDYSMLGEILPTIARYSVRDKDLGVRDLSFQLLDQILRTYYNYLIGIDWEWYKDDFYQVLQETCIKKDINTNLLLQFPPYLPHD C5B8483C3BD758C1 rbx1_yeast RING-box protein HRT1 E3 ubiquitin-protein ligase complex SCF subunit HRT1 High level expression reduces Ty3 transposition protein 1 HRT1 RBX1 ROC1 Regulator of cullins protein 1 YOL133W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNEVDRMDVDEDESQNIAQSSNQSAPVETKKKRFEIKKWTAVAFWSWDIAVDNCAICRNHIMEPCIECQPKAMTDTDNECVAAWGVCNHAFHLHCINKWIKTRDACPLDNQPWQLARCGR A9C3193E48CAF881 A9C3193E48CAF881 cdc53_yeast Cell division control protein 53 CDC53 YDL132W D2190 Cullin-A E3 ubiquitin ligase complex SCF subunit CDC53 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSETLPRSDDLEATWNFIEPGINQILGNEKNQASTSKRVYKILSPTMYMEVYTAIYNYCVNKSRSSGHFSTDSRTGQSTILVGSEIYEKLKNYLKNYILNFKQSNSETFLQFYVKRWKRFTIGAIFLNHAFDYMNRYWVQKERSDGKRHIFDVNTLCLMTWKEVMFDPSKDVLINELLDQVTLGREGQIIQRSNISTAIKSLVALGIDPQDLKKLNLNVYIQVFEKPFLKKTQEYYTQYTNDYLEKHSVTEYIFEAHEIIKREEKAMTIYWDDHTKKPLSMALNKVLITDHIEKLENEFVVLLDARDIEKITSLYALIRRDFTLIPRMASVFENYVKKTGENEISSLLAMHKHNIMKNENANPKKLALMTAHSLSPKDYIKKLLEVHDIFSKIFNESFPDDIPLAKALDNACGAFININEFALPAGSPKSATSKTSEMLAKYSDILLKKATKPEVASDMSDEDIITIFKYLTDKDAFETHYRRLFAKRLIHGTSTSAEDEENIIQRLQAANSMEYTGKITKMFQDIRLSKILEDDFAVALKNEPDYSKAKYPDLQPFVLAENMWPFSYQEVEFKLPKELVPSHEKLKESYSQKHNGRILKWLWPLCRGELKADIGKPGRMPFNFTVTLFQMAILLLYNDADVLTLENIQEGTSLTIQHIAAAMVPFIKFKLIQQVPPGLDALVKPETQFKLSRPYKALKTNINFASGVKNDILQSLSGGGHDNHGNKLGNKRLTEDERIEKELNTERQIFLEACIVRIMKAKRNLPHTTLVNECIAQSHQRFNAKVSMVKRAIDSLIQKGYLQRGDDGESYAYLA 22BC8B35034EDDEF 22BC8B35034EDDEF yd306_yeast F-box protein YDR306C YDR306C protein protein yeast Saccharomyces cerevisiae Baker's yeast MANKSRPKKIKAPYRKYVAGEGFSSTRNDNKAKEFTITIPEDAELIETPQGSYYYDETNDTIVKLTRLSNEKKDKKGRKQSPSSSSTSSSKGEKNGKVIESEEARMHSVSVKMVLPWEIQHRIIHYLDIPEKEEKLNKTANGKKTTTGINMNYLLVCRNWYAMCLPKLYYAPALTSKNFNGFVDTIIINKKKNLGHYVFELNLSTILQSGRNSFVSKLLRRCCSNLTKFIAPQTSFGYAPLISLKSCHDLKFLDLGLVSETVKLKELFSAIKNFTKLTHLSFPRSSIDCQGFQDIQWPQNLRYLKLSGGITNEFVIDTKWPTTITTLEFSYCPQITELSIYSLLSQIGDNLKHLFFHYPMPSLAENSLDHVFTYCANLISLQLMVDYCSKWCFSEFMLSKLVEYDRPLKTLYLECSGSLGLASKIHPDDLTIAILESRLPCLKNICVSPKLGWNMKSDEVADLVVSLEDQDGSLYLNY F0F69FBE96FD0155 F0F69FBE96FD0155 das1_yeast F-box protein DAS1 YJL149W J0634 DAS1 DST1-delta 6-azauracil sensitivity protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPFQDYFQKKKAAFINRNNKSNADASALRDINDININFAAKSKNYVFPLTKLPDELMQEVFSHLPQPDRLQLCLVNKRLNKIATKLLYRRIYLNDSNVVKSDFMHLAINWTLLNLPSSLKEEESRDIANCKLKKLIETLQNNIHITEVIQWIRINWDLDSTLQRSILSILCNQGKSLQRLENVTDPACNDIISNGHFSRSNVSSFDMAPPNSLPEMVVPENYIPNLTKYLSQRISSRLSHMTLFIDPLKLFNYLYPLDIKLQIIDLKLHWRREFYNNDYFVKKIRPGNPLTKLSEVFDKRTLKILTIISWNDTLLKRETEMLKDFKEFENLEDLSLISIKQDVHILVDLFSSLTNLKRLKMDFLEEYVPEPTNPHIFLSILLACSKLQFIDLRYDGLIPQIINIQENKFQLNQQCNCTNCQIVFSDILKGKIFMFPEDYYIHDLQDIAAKDIFKMMKYLSLLPYSKACDAYPSVRTQPMNLTNFVTKMNRNLLEYRNSKSQLVPKIVNNPHQHSTVTSTSTAHMSEPEMIIIDDDDDDDEINAAIPPSSDDTAATISTDLELPHESLTKRDIIMCYHALIHHFKSIYVTFLKSFPHLRFLMLNDIPTIVMEENNERIFEPVFYHYDYKSNLYGWSKESNKNLENDSNNNNNNSDTIARIATVM C48B6CAB40920027 C48B6CAB40920027 cdc4_yeast Cell division control protein 4 CDC4 YFL009W F-box protein CDC4 E3 ubiquitin ligase complex SCF subunit CDC4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGSFPLAEFPLRDIPVPYSYRVSGGIASSGSVTALVTAAGTHRNSSTAKTVETEDGEEDIDEYQRKRAAGSGESTPERSDFKRVKHDNHKTLHPVNLQNTGAASVDNDGLHNLTDISNDAEKLLMSVDDGSAAPSTLSVNMGVASHNVAAPTTVNAATITGSDVSNNVNSATINNPMEEGALPLSPTASSPGTTTPLAKTTKTINNNNNIADLIESKDSIISPEYLSDEIFSAINNNLPHAYFKNLLFRLVANMDRSELSDLGTLIKDNLKRDLITSLPFEISLKIFNYLQFEDIINSLGVSQNWNKIIRKSTSLWKKLLISENFVSPKGFNSLNLKLSQKYPKLSQQDRLRLSFLENIFILKNWYNPKFVPQRTTLRGHMTSVITCLQFEDNYVITGADDKMIRVYDSINKKFLLQLSGHDGGVWALKYAHGGILVSGSTDRTVRVWDIKKGCCTHVFKGHNSTVRCLDIVEYKNIKYIVTGSRDNTLHVWKLPKESSVPDHGEEHDYPLVFHTPEENPYFVGVLRGHMASVRTVSGHGNIVVSGSYDNTLIVWDVAQMKCLYILSGHTDRIYSTIYDHERKRCISASMDTTIRIWDLENIWNNGECSYATNSASPCAKILGAMYTLQGHTALVGLLRLSDKFLVSAAADGSIRGWDANDYSRKFSYHHTNLSAITTFYVSDNILVSGSENQFNIYNLRSGKLVHANILKDADQIWSVNFKGKTLVAAVEKDGQSFLEILDFSKASKINYVSNPVNSSSSSLESISTSLGLTRTTIIP 0348F2F8FA78F3BC 0348F2F8FA78F3BC lag2-cdc53 3 LAG2 7 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined CDC53 9 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association mint f1e f1g lag2-cdc4 3 CDC53 9 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified RBX1 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified CDC4 12 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified LAG2 7 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SKP1 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified physical association physical association f1d homomint mint yb9w-cdc53 3 CDC53 9 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level LAG2 5 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f1e mint yjo9-lag2 2 YJL149W 11 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined LAG2 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f1a mint yjn1-lag2 2 YJL131C 6 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined LAG2 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f1a mint yd306-lag2 2 YDR306C 10 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined LAG2 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f1a mint cdc53-lag2 2 CDC53 9 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined LAG2 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f1a mint yb9w-cdc53-1 1 CDC34 5 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined CDC53 9 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified physical association physical association f7 mint