MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Structure and function of a novel endonuclease acting on branched DNA substrates. Structure and function of a novel endonuclease acting on branched DNA substrates. EMBO J. (0261-4189) 2009 imex curation Ren B., Kühn J., Meslet-Cladiere L., Briffotaux J., Norais C., Lavigne R., Flament D., Ladenstein R., Myllykallio H. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ren B., Kühn J., Meslet-Cladiere L., Briffotaux J., Norais C., Lavigne R., Flament D., Ladenstein R., Myllykallio H. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation Structure and function of a novel endonuclease acting on branched DNA substrates. Structure and function of a novel endonuclease acting on branched DNA substrates. EMBO J. (0261-4189) 2009 imex curation Ren B., Kühn J., Meslet-Cladiere L., Briffotaux J., Norais C., Lavigne R., Flament D., Ladenstein R., Myllykallio H. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ren B., Kühn J., Meslet-Cladiere L., Briffotaux J., Norais C., Lavigne R., Flament D., Ladenstein R., Myllykallio H. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation Structure and function of a novel endonuclease acting on branched DNA substrates. Structure and function of a novel endonuclease acting on branched DNA substrates. EMBO J. (0261-4189) 2009 imex curation Ren B., Kühn J., Meslet-Cladiere L., Briffotaux J., Norais C., Lavigne R., Flament D., Ladenstein R., Myllykallio H. Only protein-protein interactions in vitro In vitro dls dynamic light scattering dls predetermined predetermined participant predetermined Ren B., Kühn J., Meslet-Cladiere L., Briffotaux J., Norais C., Lavigne R., Flament D., Ladenstein R., Myllykallio H. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation Structure and function of a novel endonuclease acting on branched DNA substrates. Structure and function of a novel endonuclease acting on branched DNA substrates. EMBO J. (0261-4189) 2009 imex curation Ren B., Kühn J., Meslet-Cladiere L., Briffotaux J., Norais C., Lavigne R., Flament D., Ladenstein R., Myllykallio H. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Ren B., Kühn J., Meslet-Cladiere L., Briffotaux J., Norais C., Lavigne R., Flament D., Ladenstein R., Myllykallio H. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation Structure and function of a novel endonuclease acting on branched DNA substrates. Structure and function of a novel endonuclease acting on branched DNA substrates. EMBO J. (0261-4189) 2009 imex curation Ren B., Kühn J., Meslet-Cladiere L., Briffotaux J., Norais C., Lavigne R., Flament D., Ladenstein R., Myllykallio H. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Ren B., Kühn J., Meslet-Cladiere L., Briffotaux J., Norais C., Lavigne R., Flament D., Ladenstein R., Myllykallio H. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation rfcs_pyrab Replication factor C small subunit rfcS Clamp loader small subunit PabRFC small subunit PAB0068 PYRAB01100 protein protein pyrab Pyrococcus abyssi (strain GE5 / Orsay) MRDMEEVREVKVLEKPWVEKYRPQKLEEIVGQEHIVKRLKHYVKTGSMPHLLFAGPPGVGKCLTGDAKVIANGELTTIGELVERISNGKLGPTPVRGLTVLGIDEDGKLVELPVEYVYKDKTSELVKIRTRLGRELKVTPYHPLLVNRRNGKIEWVKAEELKPGDRLAIPSFLPAMLNDNPLAEWLGYFFGNGYTDSEERVVFESKSKELRKRFMELTRKLFQDAEIKEDSGKVYVSSSEVKRLVKSLNKDSIPEQAWKGLRSFLRAYFDCNAEIKDKIIVSTAGKEIAEQISYALAGLGIVAEVDDKGSVIISDPENVSRFLDEIGFSVEEKKEEAKALIKKSTLNLGIYVDKELISYVREKLKLSFYENETMWSPEKAREIAWKLMKEIYYRLDELERFKKALSKSVIIDWSEVEKKKEEISEKTGISVNEILEYAKGKRKPSLEEYVKIAKALGVELKETLEAIFTFGKKYLGYVISDEIETLEEVRKEELKRLKELLNDEKLKKGVAYLIFLAQNELLWDEIIEVEKLKGDFVIYDLHVPKYHNFIGGNLPTVLHNTTAALALARELFGENWRHNFLELNASDERGINVIREKVKEFARTKPIGGASFKIIFLDEADALTQDAQQALRRTMEMFSSNVRFILSCVTGDTKVYTPDEREVKIRDFMNYFENGLIKEVSNRIGRDTVIAAVSFNSRIVGHPVYRLTLESGRIIEATGDHMFLTPEGWKQTYDIKEGSEVLVKPTLEGTPYEPDPRVIIDIKEFYNFLEKIEREHNLKPLKEAKTFRELITKDKEKILRRALELRAEIENGLTKREAEILELISADTWIPRAELEKKARISRTRLNQILQRLEKKGYIERRIEGRKQFVRKIRNGKILRNAMDIKRILEEEFGIKISYTTVKKLLSGNVDGMAYRILKEVKEKWLVRYDDEKAGILARVVGFILGDGHLARNGRIWFNSSKEELEMLANDLRKLGLKPSEIIERDSSSEIQGRKVKGRIYMLYVDNAAFHALLRFWKVEVGNKTKKGYTVPEWIKKGNLFVKREFLRGLFGADGTKPCGKRYNFNGIKLEIRAKKESLERTVEFLNDVADLLREFDVDSKITVSPTKEGFIIRLIVTPNDANYLNFLTRVGYAYAKDTYARLVGEYIRIKLAYKNIILPGIAEKAIELATVTNSTYAAKVLGVSRDFVVNRLKGTQIGITRDFMTFEEFMKERVLNGYVIEKVIKKEKLGYLDVYDVTCARDHSFISNGLVSHNCNYSSKIIEPIQSRCAIFRFRPLNDEDIAKRLRYIAENEGLELTEEGLQAILYIAEGDMRRAINILQAAAALDRKITDENVFLVASRARPEDIREMMLLALKGNFLKAREKLREILLKQGLSGEDVLVQMHREVFNLPIDEPMKVYLADKIGEYNFRLVEGANEMIQLEALLAQFTLIGKKK mint 9725AC4F4240C985 nucs_pyrab Endonuclease NucS nucS Nuclease for ssDNA PAB2263 PYRAB01260 protein protein pyrab Pyrococcus abyssi (strain GE5 / Orsay) MRKVIIKENPSEEEIKELLDLAEKHGGVVTIFARCKVHYEGRAKSELGEGDRIIIIKPDGSFLIHQNKKREPVNWQPPGSKVTFKENSIISIRRRPYERLEVEIIEPYSLVVFLAEDYEELALTGSEAEMANLIFENPRVIEEGFKPIYREKPIRHGIVDVMGVDKDGNIVVLELKRRKADLHAVSQLKRYVDSLKEEYGENVRGILVAPSLTEGAKKLLEKEGLEFRKLEPPKKGNEKRSKQKTLDFFTP mint 648BB6A7CE1E0EFC top6b_pyrab Type 2 DNA topoisomerase 6 subunit B top6B Type II DNA topoisomerase VI subunit B PAB0407 PYRAB05940 protein protein pyrab Pyrococcus abyssi (strain GE5 / Orsay) MAEARDLFKEFKVQSVSEFFRRNAAMLGYTGKIRSLTTIIHEAVTNSLDACEEAGILPYIRVEIEELGKEHYKVIVEDNGPGIPEEYIPHVFGKMLAGTKAHRNIQSRGQQGIGISGAVMFAQITSGKATRVITSTGGEIVEAWVKIDVQKNEGKIVKKLKHPNPKGWRGTRIELEVKDVKYVRSKQGVYWYLKLTAIANPHAHIELVEPDGKLVVFPRSSEDIPEPPVEMKPHPKGVMVDDVYTMAHRSKRSSVRRFLVSEFSRISDKKVDELIKYITALRLIKSETNKEVKEKLYEKLVSGDVDSVLRAFGRKWKKELEKVAKIMDKSPEKLTWHEAEEIVEAFKLMKFLAPPTHGLRPIGEENIKKGLTSILKPEFVTAVTRAPRVYAGGIPFQVEVGLAYGGQIQGSEILRYANRVPLLFDAGSCVITSAVRSIDWKRYKIDSFDSAPLVVLVNVVSVHVPYTSTGKQSIADIDEIHNEIRLALMDAARRLSFYLGGKFRRMYQVKRRKTLEKYLPEIARSLHILTGEPEEKIKEYFLKLIESKIEVEEVSEVEAEEAEA mint 267C0D89DD4C1F22 pcna_pyrab DNA polymerase sliding clamp pcn Proliferating cell nuclear antigen homolog PAB1465 PYRAB13790 protein protein pyrab Pyrococcus abyssi (strain GE5 / Orsay) MPFEIVFEGAKEFAQLIETASRLIDEAAFKVTEEGISMRAMDPSRVVLIDLNLPASIFSKYEVDGEETIGVNMDHLKKVLKRGKAKETLILRKGEENFLEISLQGTATRTFKLPLIDVEEIEVDLPELPFTAKVVILGDVIKEAVKDASLVSDSMKFIAKENEFTMRAEGETQEVEVKLTLEDEGLLDIEVQEETKSAYGISYLSDMVKGLGKADEVTIKFGNEMPMQMEYYIRDEGRLIFLLAPRVEE mint B7E21A135B3F3BC8 rfcl_pyrab Replication factor C large subunit rfcL Clamp loader large subunit PabRFC large subunit PAB0069 PYRAB01110 protein protein pyrab Pyrococcus abyssi (strain GE5 / Orsay) MPEVPWVEKYRPRRLSEIINQEDAISKVKAWIEAWLHGNPPKKKALLLAGPPGSGKTTTVYALAREYNFEVIELNASDERTYDKIARYVQAAYTMDILGKRRKIIFLDEADNIEPSGAPEIAKLIDRARNPIIMAANHYWEVPKEIRDRAELVEYKRLTQRDVINALVRILKREGITVPKEVLVEIAKRASGDLRAAINDLQTVVAGGYEDARYVLAYRDVEKTVFQALGLVFASDNAKRAKLAMMNLDMSPDEFLLWIDENIPHMYLKPEEMARAYEAISKADIYLGRAQRTGNYSLWRYAIDMMTAGVAVAGTKKRGFAKFYPPNTLKMLAESKEERSTRNSIIKKIMSEMHMSKLEAIETMKIIKTIFEKNLDLAAHFTVFLGLSEKEVEFLAGRENAGTIWGKALAIRRKLKKEEEKIRKERKEEEKVEVREEKPEEKVEEKREERETKKEKEKKEEKKAEKKGKQVTLFDFIKK mint A29BB46E376FBF62 q9v1z5_pyrab PAB0190 protein protein pyrab Pyrococcus abyssi (strain GE5 / Orsay) MNTMYLRKELLQPRLYQELIYAKCKERNCLIVLPTGLGKTIIAMMIADYRLNKYGGKVLMLAPTKPLVLQHAETFRKFFNLPPEKIVALTGEVSPSERVKAWNRAKVIIATPQTVENDLLTGKINLEDVVLVIFDEAHRAVGNYAYVYIAREYLNQAKNPHVIGLTASPGSSQEKILEVLRNLGIEHIEYRSENSPDVKPYVQGIKFEWIKVELPGLYKEVRKLLRDMLRDSLKPLAEAGLIESASADLPKKEILRAGQIINEETAKGNHDLRKLLLFHAMALKLHHAIELLETQGLSALRVYLKKLYEEAKTGSTRASKELFLDRRMKKALALLIQAKELGIDHPKMEVLKELVKEQLSKKENSKIIVFTNYRETARKVVEELTKEGIKAKRFVGQATKENDRGMSQREQKLILDSFARGEFNVLVATSVGEEGLDVPEVDLVVFYEPVPSAIRSVQRRGRTGRQKPGRVVILIAQGTRDEAYYWSSKHKERQMRETIRMVSQAIKREKQLSLESYVKKDTEKEMEREEKEERKGEREGVKVVVDSRELRSEVVKRLKTLGVKIEVKTLDVGDYIISDEVAIERKSANDFIQSIIDGRLFDQVKRLKDSYPRPVVIVEGQLYGIRNVHPNAIRGAIASIVIDFGVPIIFSSNPEETANYIFFMARREQEERRREVRVRGEKKALTLAERQRLIVESLPHVSATLAKRLLKHFGSVERVFTASVPELMKVEGIGEKIAREIRRVITSPYTED mint 2B1839EC2CFC97B4 top6a_pyrab Type 2 DNA topoisomerase 6 subunit A Type II DNA topoisomerase VI subunit A PAB2411 PYRAB05950 top6A protein protein pyrab Pyrococcus abyssi (strain GE5 / Orsay) MKLKRQKPKEKFSYDPQKVLKKLEDLAWKILEEVKSGKNPYFDVPTRGLNNVYFDEEARLIKLGDKLSRRYFLNVAHARKFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTRENTFEDQSESDPIIEDLERMLGVLREEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWAVPGTVEHIQFPEVNVDYVLVVETAAMADRLIEEKYPKKENCLIVATQGQASRGVRRLIHRLHYEEGLPIIVFTDGDPYGWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNLEHVTEKLKGIPPDKKGGPTGDYKRLIEELNYPWFQNKEWQRQLKLALKWGVRIEQQALANKSLEFVAKEYLPEKIREGKLLP mint 6462F819FDEBD6BD pcn-nucs 4 PCNA 9 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified NucS 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction f1c mint nucs-pcn 5 NucS 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined DNA topoisomerase 6 subunit B 8 ms participant Identification by mass spectrometry ms participant predetermined predetermined participant predetermined unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level Replication factor C small subunit 6 ms participant Identification by mass spectrometry ms participant predetermined predetermined participant predetermined unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level PCNA 9 ms participant Identification by mass spectrometry ms participant predetermined predetermined participant predetermined unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level Hef 11 ms participant Identification by mass spectrometry ms participant predetermined predetermined participant predetermined unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level Replication factor C large subunit 10 ms participant Identification by mass spectrometry ms participant predetermined predetermined participant predetermined unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level DNA topoisomerase VI subunit A 12 ms participant Identification by mass spectrometry ms participant predetermined predetermined participant predetermined unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level association association sf1 mint nucs-pcn-1 5 NucS 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PCNA 9 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f1c mint nucs-2 2 NucS 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 direct interaction direct interaction f2d mint nucs-2 2 NucS 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 4.0 direct interaction direct interaction f2d mint The crystal NucS dimer further aggregate to form tetramers because of crystallografic two-fold symmetry nucs-2 1 NucS 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 direct interaction direct interaction f1e mint nucs-2 3 NucS 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 direct interaction direct interaction f1d mint