I2D I2D A subcortical maternal complex essential for preimplantation mouse embryogenesis. A subcortical maternal complex essential for preimplantation mouse embryogenesis. Dev. Cell (1534-5807) 2008 imex curation Li L., Baibakov B., Dean J. jurrien@helix.nih.gov,lile@mail.nih.gov 21-Dec-2010: Contacted by JYOTI. orchard Only protein-protein interactions Curation by: I2D - Interologous Interaction Database mouse-morula Mus musculus morula morula mass of blastomeres preceding blastula stage confocal microscopy confocal microscopy immunostaining immunostaining Li L., Baibakov B., Dean J. jurrien@helix.nih.gov jurrien@helix.nih.gov,lile@mail.nih.gov Accepted 2010-OCT-9 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Dev. Cell (1534-5807) 2008 orchard Only protein-protein interactions imex curation Curation by: I2D - Interologous Interaction Database A subcortical maternal complex essential for preimplantation mouse embryogenesis. A subcortical maternal complex essential for preimplantation mouse embryogenesis. Dev. Cell (1534-5807) 2008 imex curation Li L., Baibakov B., Dean J. jurrien@helix.nih.gov,lile@mail.nih.gov 21-Dec-2010: Contacted by JYOTI. orchard Only protein-protein interactions Curation by: I2D - Interologous Interaction Database mouse-blastocyst Mus musculus blastocyst blastocyst blastocyst confocal microscopy confocal microscopy immunostaining immunostaining Li L., Baibakov B., Dean J. jurrien@helix.nih.gov jurrien@helix.nih.gov,lile@mail.nih.gov Accepted 2010-OCT-9 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Dev. Cell (1534-5807) 2008 orchard Only protein-protein interactions imex curation Curation by: I2D - Interologous Interaction Database A subcortical maternal complex essential for preimplantation mouse embryogenesis. A subcortical maternal complex essential for preimplantation mouse embryogenesis. Dev. Cell (1534-5807) 2008 imex curation Li L., Baibakov B., Dean J. jurrien@helix.nih.gov,lile@mail.nih.gov 21-Dec-2010: Contacted by JYOTI. orchard Only protein-protein interactions Curation by: I2D - Interologous Interaction Database mouse-ovary Mouse ovary ovary Ovary anti bait coip anti bait coimmunoprecipitation anti bait coip sequence tag sequence tag identification sequence tag Li L., Baibakov B., Dean J. jurrien@helix.nih.gov jurrien@helix.nih.gov,lile@mail.nih.gov Accepted 2010-OCT-9 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Dev. Cell (1534-5807) 2008 orchard Only protein-protein interactions imex curation Curation by: I2D - Interologous Interaction Database A subcortical maternal complex essential for preimplantation mouse embryogenesis. A subcortical maternal complex essential for preimplantation mouse embryogenesis. Dev. Cell (1534-5807) 2008 imex curation Li L., Baibakov B., Dean J. jurrien@helix.nih.gov,lile@mail.nih.gov 21-Dec-2010: Contacted by JYOTI. orchard Only protein-protein interactions Curation by: I2D - Interologous Interaction Database chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Li L., Baibakov B., Dean J. Type of COS cells not specified. Author confirms that all constructs for Floped, Filia, Mater and Tle-6 are mouse. jurrien@helix.nih.gov jurrien@helix.nih.gov,lile@mail.nih.gov Accepted 2010-OCT-9 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Dev. Cell (1534-5807) 2008 orchard Only protein-protein interactions imex curation Curation by: I2D - Interologous Interaction Database A subcortical maternal complex essential for preimplantation mouse embryogenesis. A subcortical maternal complex essential for preimplantation mouse embryogenesis. Dev. Cell (1534-5807) 2008 imex curation Li L., Baibakov B., Dean J. jurrien@helix.nih.gov,lile@mail.nih.gov 21-Dec-2010: Contacted by JYOTI. orchard Only protein-protein interactions Curation by: I2D - Interologous Interaction Database mouse-ovum Mus musculus egg cell ovum egg cell molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Li L., Baibakov B., Dean J. Superose 6 10/300 GL column jurrien@helix.nih.gov jurrien@helix.nih.gov,lile@mail.nih.gov Accepted 2010-OCT-9 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Dev. Cell (1534-5807) 2008 orchard Only protein-protein interactions imex curation Curation by: I2D - Interologous Interaction Database A subcortical maternal complex essential for preimplantation mouse embryogenesis. A subcortical maternal complex essential for preimplantation mouse embryogenesis. Dev. Cell (1534-5807) 2008 imex curation Li L., Baibakov B., Dean J. jurrien@helix.nih.gov,lile@mail.nih.gov 21-Dec-2010: Contacted by JYOTI. orchard Only protein-protein interactions Curation by: I2D - Interologous Interaction Database mouse-ovum Mus musculus egg cell ovum egg cell confocal microscopy confocal microscopy immunostaining immunostaining Li L., Baibakov B., Dean J. jurrien@helix.nih.gov jurrien@helix.nih.gov,lile@mail.nih.gov Accepted 2010-OCT-9 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Dev. Cell (1534-5807) 2008 orchard Only protein-protein interactions imex curation Curation by: I2D - Interologous Interaction Database A subcortical maternal complex essential for preimplantation mouse embryogenesis. A subcortical maternal complex essential for preimplantation mouse embryogenesis. Dev. Cell (1534-5807) 2008 imex curation Li L., Baibakov B., Dean J. jurrien@helix.nih.gov,lile@mail.nih.gov 21-Dec-2010: Contacted by JYOTI. orchard Only protein-protein interactions Curation by: I2D - Interologous Interaction Database mouse-embryo Mus musculus embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. confocal microscopy confocal microscopy immunostaining immunostaining Li L., Baibakov B., Dean J. jurrien@helix.nih.gov jurrien@helix.nih.gov,lile@mail.nih.gov Accepted 2010-OCT-9 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Dev. Cell (1534-5807) 2008 orchard Only protein-protein interactions imex curation Curation by: I2D - Interologous Interaction Database A subcortical maternal complex essential for preimplantation mouse embryogenesis. A subcortical maternal complex essential for preimplantation mouse embryogenesis. Dev. Cell (1534-5807) 2008 imex curation Li L., Baibakov B., Dean J. jurrien@helix.nih.gov,lile@mail.nih.gov 21-Dec-2010: Contacted by JYOTI. orchard Only protein-protein interactions Curation by: I2D - Interologous Interaction Database chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li L., Baibakov B., Dean J. Type of COS cells not specified Author confirms that all constructs used for Floped, Filia, Mater and Tle-6 are mouse. jurrien@helix.nih.gov jurrien@helix.nih.gov,lile@mail.nih.gov Accepted 2010-OCT-9 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Dev. Cell (1534-5807) 2008 orchard Only protein-protein interactions imex curation Curation by: I2D - Interologous Interaction Database A subcortical maternal complex essential for preimplantation mouse embryogenesis. A subcortical maternal complex essential for preimplantation mouse embryogenesis. Dev. Cell (1534-5807) 2008 imex curation Li L., Baibakov B., Dean J. jurrien@helix.nih.gov,lile@mail.nih.gov 21-Dec-2010: Contacted by JYOTI. orchard Only protein-protein interactions Curation by: I2D - Interologous Interaction Database mouse-zygote Mus musculus zygote zygote fertilised egg cell confocal microscopy confocal microscopy immunostaining immunostaining Li L., Baibakov B., Dean J. jurrien@helix.nih.gov jurrien@helix.nih.gov,lile@mail.nih.gov Accepted 2010-OCT-9 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Dev. Cell (1534-5807) 2008 orchard Only protein-protein interactions imex curation Curation by: I2D - Interologous Interaction Database A subcortical maternal complex essential for preimplantation mouse embryogenesis. A subcortical maternal complex essential for preimplantation mouse embryogenesis. Dev. Cell (1534-5807) 2008 imex curation Li L., Baibakov B., Dean J. jurrien@helix.nih.gov,lile@mail.nih.gov 21-Dec-2010: Contacted by JYOTI. orchard Only protein-protein interactions Curation by: I2D - Interologous Interaction Database mouse-ovary Mouse ovary ovary Ovary anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li L., Baibakov B., Dean J. jurrien@helix.nih.gov jurrien@helix.nih.gov,lile@mail.nih.gov Accepted 2010-OCT-9 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Dev. Cell (1534-5807) 2008 orchard Only protein-protein interactions imex curation Curation by: I2D - Interologous Interaction Database nalp5_mouse NACHT, LRR and PYD domains-containing protein 5 Nlrp5 Mater Nalp5 Maternal antigen that embryos require Ooplasm-specific protein 1 protein protein mouse Mus musculus Mouse MGPPEKESKAILKARGLEEEQKSERKMTSPENDSKSIQKDQGPEQEQTSESTMGPPEKESKAILKARGLEEEQKSERKMTSPENDSKSIQKDQGPEQEQTSESTMGPPEKDSKAILKARGLEEEQKSESTMSPSENVSRAILKDSGSEEVEQASERKMTSPENDSKSIQKDQGPEQEQTSETLQSKEEDEVTEADKDNGGDLQDYKAHVIAKFDTSVDLHYDSPEMKLLSDAFKPYQKTFQPHTIILHGRPGVGKSALARSIVLGWAQGKLFQKMSFVIFFSVREIKWTEKSSLAQLIAKECPDSWDLVTKIMSQPERLLFVIDGLDDMDSVLQHDDMTLSRDWKDEQPIYILMYSLLRKALLPQSFLIITTRNTGLEKLKSMVVSPLYILVEGLSASRRSQLVLENISNESDRIQVFHSLIENHQLFDQCQAPSVCSLVCEALQLQKKLGKRCTLPCQTLTGLYATLVFHQLTLKRPSQSALSQEEQITLVGLCMMAAEGVWTMRSVFYDDDLKNYSLKESEILALFHMNILLQVGHNSEQCYVFSHLSLQDFFAALYYVLEGLEEWNQHFCFIENQRSIMEVKRTDDTRLLGMKRFLFGLMNKDILKTLEVLFEYPVIPTVEQKLQHWVSLIAQQVNGTSPMDTLDAFYCLFESQDEEFVGGALKRFQEVWLLINQKMDLKVSSYCLKHCQNLKAIRVDIRDLLSVDNTLELCPVVTVQETQCKPLLMEWWGNFCSVLGSLRNLKELDLGDSILSQRAMKILCLELRNQSCRIQKLTFKSAEVVSGLKHLWKLLFSNQNLKYLNLGNTPMKDDDMKLACEALKHPKCSVETLRLDSCELTIIGYEMISTLLISTTRLKCLSLAKNRVGVKSMISLGNALSSSMCLLQKLILDNCGLTPASCHLLVSALFSNQNLTHLCLSNNSLGTEGVQQLCQFLRNPECALQRLILNHCNIVDDAYGFLAMRLANNTKLTHLSLTMNPVGDGAMKLLCEALKEPTCYLQELELVDCQLTQNCCEDLACMITTTKHLKSLDLGNNALGDKGVITLCEGLKQSSSSLRRLGLGACKLTSNCCEALSLAISCNPHLNSLNLVKNDFSTSGMLKLCSAFQCPVSNLGIIGLWKQEYYARVRRQLEEVEFVKPHVVIDGDWYASDEDDRNWWKN EF14379B454E9376 ooep_mouse Oocyte-expressed protein homolog Ooep Oep19 Sddr Oocyte- and embryo-specific protein 19 STAT3 downstream gene and differentiation regulator Factor located in oocytes permitting embryonic development protein protein mouse Mus musculus Mouse MASHTADADAKPDSDSQKLLNVLPVSLRLRTRPWWFPIQEVSNPLVLYMEAWVAERVIGTDQAEISEIEWMCQALLTVDSVNSGNLAEITIFGQPSAQTRMKNILLNMAAWHKENELQRAVKVKEVEEFLKIRASSILSKLSKKGLKLAGFPLPLEGRETQMES 95B121777F282D39 khdc3_mouse KH domain-containing protein 3 Khdc3 Protein Filia protein protein mouse Mus musculus Mouse MASLKRFQTLVPLDHKQGTLFEIIGEPKLPKWFHVECLEDPKRLYVEPRLLEIMFGKDGEHIPHLESMLHTLIHVNVWGPERRAEIWIFGPPPFRRDVDRMLTDLAHYCRMKLMEIEALEAGVERRRMAAHKAATQPAPVKVREAAPRPASVKVPETATQPAPVKVREAAPQPAPVQEVREAAPQQASVQEEVREAATEQAPVQEVREAATEQAPVQEVSEAATEQAPVQEVNEAATEQASVQAVREAATRPAPGKVRKAATQPAPVQVCQEATQLAPVKVREAATQPASGKVREAATQLAPVKVRKAATQLAPVKVHEAATQPAPGKVSDAATQSASVQVREAATQLSPVEATDTSQLAQVKADEAFAQHTSGEAHQVANGQSPIEVCETATGQHSLDVSRALSQKCPEVFEWETQSCLDGSYVIVQPPRDAWESFIIL 19C1B93DACC42851 tle6_mouse Transducin-like enhancer protein 6 Tle6 Grg6 Groucho-related protein 6 protein protein mouse Mus musculus Mouse MTSHRQSSDTFGGILPSTLSSRYLSIVNQLPEEFSSVVSEMMVHLENIFSLAENFFQAIERFSRTPDLLERNKMSIGVGAEGDSWPCHVSHEAPMGSAQTTENSAKEEDKQVPESAALQHPKFKSTPGPQLPTRRRFLSESDELQDPQPVWDAEPQFCQGFLIQGLWELFMDSRQKNQQEHGGEDSSQESKDSGLCDFKPEPQPRHRNSLSDSADPFLIKSPSALLDYYQEDVSRPQPETQESSGRADKFLKPLSWGSEVLESSCNQPSTALWQLERFTVPQALQKVRVLKHQELLLVVAVSSFTRHVFTCSQSGIKVWNLVNQVAEDRDPESHLKCSVQDNKVYLRTCLLSSNSRTLFAGGYNLPGVIVWDLAAPSLYEKCQLPCEGLSCQALANTKENMALAGFTDGTVRIWDLRTQEIVRNLKGPTNSARNLVVKDDNIWTGGLDACLRCWDLRMAKVSLEHLFQSQIMSLAHSPTEDWLLLGLANGQHCLFNSRKRDQVLTVDTKDNTILGLKFSPNGKWWASVGMGNFITVHSMPTGAKLFQVPEVGPVRCFDMTENGRLIITGSRDCASVYHIKY 946369E084B800F6 ooep-nlrp5 3 11 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait association association Fig 3A ooep-nlrp5-1 10 13 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey association association Fig 4A ooep-nlrp5-2 10 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait association association Fig 4a ooep-nlrp5-3 4 12 unspecified role unspecified role bait bait region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role prey prey association association Fig 4b ooep-nlrp5-4 4 13 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role prey prey association association Fig 4c filia-nlrp5-1 4 13 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig 4e ooep-nlrp5-6 8 11 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig 4d ooep-nlrp5-7 5 13 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component physical association physical association Fig 4f ooep-nlrp5-8 6 12 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 5a ooep-nlrp5-9 9 12 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 5a ooep-nlrp5-10 7 12 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 5a ooep-nlrp5-11 1 11 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 5a ooep-nlrp5-12 2 12 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 5a