IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Agonist mediated internalization of M2 mAChR is beta-arrestin-dependent Agonist mediated internalization of M2 mAChR is beta-arrestin-dependent J Mol Signal. 2006 imex curation Kymry T Jones, Maria Echeverry, Valerie A Mosser, Alicia Gates and Darrell A Jackson Source of beta arrestin taken from back reference PMID: 10725388 darrell.jackson@umontana.edu 13-Sept-2010: Contacted by JYOTI. orchard Only protein-protein interactions Curation by: Molecular Connections Pvt. Ltd rat-aortic_smooth_muscle rat aortic smooth muscle cells aortic_smooth_muscle aortic smooth muscle cells confocal microscopy confocal microscopy immunostaining immunostaining Kymry T Jones, Maria Echeverry, Valerie A Mosser, Alicia Gates and Darrell A Jackson Source of beta arrestin taken from back reference PMID: 10725388 darrell.jackson@umontana.edu Accepted 2010-AUG-30 by ORCHARD. J Mol Signal. 2006 orchard Only protein-protein interactions imex curation Curation by: Molecular Connections Pvt. Ltd Agonist mediated internalization of M2 mAChR is beta-arrestin-dependent Agonist mediated internalization of M2 mAChR is beta-arrestin-dependent J Mol Signal. 2006 imex curation Kymry T Jones, Maria Echeverry, Valerie A Mosser, Alicia Gates and Darrell A Jackson Source of beta arrestin taken from back reference PMID: 10725388 darrell.jackson@umontana.edu 13-Sept-2010: Contacted by JYOTI. orchard Only protein-protein interactions Curation by: Molecular Connections Pvt. Ltd chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 confocal microscopy confocal microscopy immunostaining immunostaining Kymry T Jones, Maria Echeverry, Valerie A Mosser, Alicia Gates and Darrell A Jackson Source of beta arrestin taken from back reference PMID: 10725388 darrell.jackson@umontana.edu Accepted 2010-AUG-30 by ORCHARD. 13-Sept-2010: Contacted by JYOTI. J Mol Signal. 2006 orchard Only protein-protein interactions imex curation Curation by: Molecular Connections Pvt. Ltd Agonist mediated internalization of M2 mAChR is beta-arrestin-dependent Agonist mediated internalization of M2 mAChR is beta-arrestin-dependent J Mol Signal. 2006 imex curation Kymry T Jones, Maria Echeverry, Valerie A Mosser, Alicia Gates and Darrell A Jackson Source of beta arrestin taken from back reference PMID: 10725388 darrell.jackson@umontana.edu 13-Sept-2010: Contacted by JYOTI. orchard Only protein-protein interactions Curation by: Molecular Connections Pvt. Ltd mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. confocal microscopy confocal microscopy immunostaining immunostaining Kymry T Jones, Maria Echeverry, Valerie A Mosser, Alicia Gates and Darrell A Jackson Source of beta arrestin taken from back reference PMID: 10725388 darrell.jackson@umontana.edu Accepted 2010-AUG-30 by ORCHARD. 13-Sept-2010: Contacted by JYOTI. J Mol Signal. 2006 orchard Only protein-protein interactions imex curation Curation by: Molecular Connections Pvt. Ltd Agonist mediated internalization of M2 mAChR is beta-arrestin-dependent Agonist mediated internalization of M2 mAChR is beta-arrestin-dependent J Mol Signal. 2006 imex curation Kymry T Jones, Maria Echeverry, Valerie A Mosser, Alicia Gates and Darrell A Jackson Source of beta arrestin taken from back reference PMID: 10725388 darrell.jackson@umontana.edu 13-Sept-2010: Contacted by JYOTI. orchard Only protein-protein interactions Curation by: Molecular Connections Pvt. Ltd human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Kymry T Jones, Maria Echeverry, Valerie A Mosser, Alicia Gates and Darrell A Jackson Source of beta arrestin taken from back reference PMID: 10725388 darrell.jackson@umontana.edu Accepted 2010-AUG-30 by ORCHARD. 13-Sept-2010: Contacted by JYOTI. J Mol Signal. 2006 orchard Only protein-protein interactions imex curation Curation by: Molecular Connections Pvt. Ltd acm2_pig Muscarinic acetylcholine receptor M2 CHRM2 protein protein pig Sus scrofa (Pig) Pig MNNSTNSSNSGLALTSPYKTFEVVFIVLVAGSLSLVTIIGNILVMVSIKVNRHLQTVNNYFLFSLACADLIIGVFSMNLYTLYTVIGYWPLGPVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPVKRTTKMAGMMIAAAWVLSFILWAPAILFWQFIVGVRTVEDGECYIQFFSNAAVTFGTAIAAFYLPVIIMTVLYWHISRASKSRIKKDKKEPVANQEPVSPSLVQGRIVKPNNNNMPGSDEALEHNKIQNGKAPRDAVTENCVQGEEKESSNDSTSVSAVASNMRDDEITQDENTVSTSLGHSKDENSKQTCIKIVTKTQKSDSCTPANTTVELVGSSGQNGDEKQNIVARKIVKMTKQPAKKKPPPSREKKVTRTILAILLAFIITWAPYNVMVLINTFCAPCIPNTVWTIGYWLCYINSTINPACYALCNATFKKTFKHLLMCHYKNIGATR 53D089F179789CD9 arrb2_rat Beta-arrestin-2 Arrb2 Arrestin beta-2 protein protein rat Rattus norvegicus (Rat) Rat MGEKPGTRVFKKSSPNCKLTVYLGKRDFVDHLDKVDPVDGVVLVDPDYLKDRKVFVTLTCAFRYGREDLDVLGLSFRKDLFIATYQAFPPMPNPPRPPTRLQDRLLKKLGQHAHPFFFTIPQNLPCSVTLQPGPEDTGKACGVDFEIRAFCAKSIEEKSHKRNSVRLIIRKVQFAPETPGPQPSAETTRHFLMSDRRSLHLEASLDKELYYHGEPLNVNVHVTNNSAKTVKKIRVSVRQYADICLFSTAQYKCPVAQLEQDDQVSPSSTFCKVYTITPLLSDNREKRGLALDGQLKHEDTNLASSTIVKEGANKEVLGILVSYRVKVKLVVSRGGDVSVELPFVLMHPKPHDHITLPRPQSAPREIDIPVDTNLIEFDTNYATDDDIVFEDFARLRLKGMKDDDCDDQFC DIP protein P29067 original sequence version: 115 DIP protein P29067 original sequence version: 112 DIP protein P29067 original sequence version: 100 0DFA6A897C2B86BA arrb2-chrm2 3 5 unspecified role unspecified role neutral component neutral component n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component c-terminus c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization fig 4 carbachol arrb2-chrm2-3 4 5 unspecified role unspecified role neutral component neutral component n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component c-terminus c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization carbachol fig 8 and fig 5 arrb2-chrm2-2 2 5 unspecified role unspecified role neutral component neutral component n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component c-terminus c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization fig 5 carbachol arrb2-chrm2-1 1 5 unspecified role unspecified role neutral component neutral component n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component c-terminus c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization carbachol fig 5