IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Camguk/CASK enhances Ether-a-go-go potassium current by a phosphorylation-dependent mechanism. Camguk/CASK enhances Ether-a-go-go potassium current by a phosphorylation-dependent mechanism. J. Neurosci. (0270-6474) 2005 imex curation Marble DD., Hegle AP., Snyder ED., Dimitratos S., Bryant PJ., Wilson GF. wilsongf@umich.edu 06-April-2011: Contacted by JYOTI. jyoti Only protein-protein interactions in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Marble DD., Hegle AP., Snyder ED., Dimitratos S., Bryant PJ., Wilson GF. wilsongf@umich.edu Accepted 2010-Not a month!-8 by JYOTI. 06-April-2011: Contacted by JYOTI. J. Neurosci. (0270-6474) 2005 jyoti Only protein-protein interactions imex curation Camguk/CASK enhances Ether-a-go-go potassium current by a phosphorylation-dependent mechanism. Camguk/CASK enhances Ether-a-go-go potassium current by a phosphorylation-dependent mechanism. J. Neurosci. (0270-6474) 2005 imex curation Marble DD., Hegle AP., Snyder ED., Dimitratos S., Bryant PJ., Wilson GF. wilsongf@umich.edu 06-April-2011: Contacted by JYOTI. jyoti Only protein-protein interactions xenla-oocyte Xenopus laevis oocyte Oocyte anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Marble DD., Hegle AP., Snyder ED., Dimitratos S., Bryant PJ., Wilson GF. wilsongf@umich.edu Accepted 2010-Not a month!-8 by JYOTI. 06-April-2011: Contacted by JYOTI. J. Neurosci. (0270-6474) 2005 jyoti Only protein-protein interactions imex curation Camguk/CASK enhances Ether-a-go-go potassium current by a phosphorylation-dependent mechanism. Camguk/CASK enhances Ether-a-go-go potassium current by a phosphorylation-dependent mechanism. J. Neurosci. (0270-6474) 2005 imex curation Marble DD., Hegle AP., Snyder ED., Dimitratos S., Bryant PJ., Wilson GF. wilsongf@umich.edu 06-April-2011: Contacted by JYOTI. jyoti Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Marble DD., Hegle AP., Snyder ED., Dimitratos S., Bryant PJ., Wilson GF. wilsongf@umich.edu Accepted 2010-Not a month!-8 by JYOTI. 06-April-2011: Contacted by JYOTI. J. Neurosci. (0270-6474) 2005 jyoti Only protein-protein interactions imex curation Camguk/CASK enhances Ether-a-go-go potassium current by a phosphorylation-dependent mechanism. Camguk/CASK enhances Ether-a-go-go potassium current by a phosphorylation-dependent mechanism. J. Neurosci. (0270-6474) 2005 imex curation Marble DD., Hegle AP., Snyder ED., Dimitratos S., Bryant PJ., Wilson GF. wilsongf@umich.edu 06-April-2011: Contacted by JYOTI. jyoti Only protein-protein interactions drome-larva Drosophila melanogaster larva larva Immature form of an organism anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Marble DD., Hegle AP., Snyder ED., Dimitratos S., Bryant PJ., Wilson GF. Interaction observed in larval nervous system, imaginal discs, and body muscle fibers. wilsongf@umich.edu Accepted 2010-Not a month!-8 by JYOTI. 06-April-2011: Contacted by JYOTI. J. Neurosci. (0270-6474) 2005 jyoti Only protein-protein interactions imex curation dlg1_drome Disks large 1 tumor suppressor protein dlg1 l(1)dlg1 CG1725 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MPVKKQEAHRALELLEDYHARLSEPQDRALRIAIERVIRIFKSRLFQALLDIQEFYELTLLDDSKSIQQKTAETLQIATKWEKDGQAVKIADFIKSSNLNRNCAYEFNNDASSNQTNQSALNQNPIANNVSAQAQAEALSRTFKSELEEILNQRMRIESDTENAKEPTVEQQQKQQQAQQRSSRSPQQQNPQQQQGSKSRSGSQTVNGDDSWLYEDIQLERGNSGLGFSIAGGTDNPHIGTDTSIYITKLISGGAAAADGRLSINDIIVSVNDVSVVDVPHASAVDALKKAGNVVKLHVKRKRGTATTPAAGSAAGDARDSAASGPKVIEIDLVKGGKGLGFSIAGGIGNQHIPGDNGIYVTKLMDGGAAQVDGRLSIGDKLIAVRTNGSEKNLENVTHELAVATLKSITDKVTLIIGKTQHLTTSASGGGGGGLSSGQQLSQSQSQLATSQSQSQVHQQQHATPMVNSQSTEPGSRYASTNVLAAVPPGTPRAVSTEDITREPRTITIQKGPQGLGFNIVGGEDGQGIYVSFILAGGPADLGSELKRGDQLLSVNNVNLTHATHEEAAQALKTSGGVVTLLAQYRPEEYNRFEARIQELKQQAALGAGGSGTLLRTTQKRSLYVRALFDYDPNRDDGLPSRGLPFKHGDILHVTNASDDEWWQARRVLGDNEDEQIGIVPSKRRWERKMRARDRSVKFQGHAAANNNLDKQSTLDRKKKNFTFSRKFPFMKSRDEKNEDGSDQEPFMLCYTQDDANAEGASEENVLSYEAVQRLSINYTRPVIILGPLKDRINDDLISEYPDKFGSCVPHTTRPKREYEVDGRDYHFVSSREQMERDIQNHLFIEAGQYNDNLYGTSVASVREVAEKGKHCILDVSGNAIKRLQVAQLYPVAVFIKPKSVDSVMEMNRRMTEEQAKKTYERAIKMEQEFGEYFTGVVQGDTIEEIYSKVKSMIWSQSGPTIWVPSKESL DIP protein P31007 original sequence version: 157 544D1E1AD03B0674 cskp_drome Peripheral plasma membrane protein CASK CASK cmg CG6703 Caki Calcium/calmodulin-dependent protein kinase protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTEDEILFDDVYELCEVIGKGPFSIVRRCIHRESNQQFAVKIVDVAKFTASPGLSTADLKREATICHMLKHPHIVELLETYSSEGMLYMVFEFMEGSDLCFEVVRRAVAGFVYSEAVACHYMRQILEALRYCHENDILHRDVRPACALLATVDNSAPVKLGGFGSAIQLPGTRETIETHGRVGCPHYMAPEVVTRRLYGKGCDVWGAGVMLHVLLSGRLPFLGSGVRLQQSVARGRLSFEAPEWKSISANAKDLVMKMLAANPHHRLSITEVLDHPWIRDRDKLQRTHLADTVEELKRYNARRKLKGAVQAIAGGTNMDPLYATDADMPITGATDEWADEEAGIEAVQRILDCLDDIYSLQDAHVDADVLRDMLRDNRLHQFLQLFDRIAATVVTSNGRAPAAEAVGRCRDVLEQLSSTSGGNSLGGKYAKEELMRLLAAPHMQALLHSHDVVARDVYGEEALRVTPPPMVPYLNGDELDNVEGGELQHVTRVRLVQFQKNTDEPMGITLKMTEDGRCIVARIMHGGMIHRQATLHVGDEIREINGQPVQHQSVGQLQRMLREARGSVTFKIVPSYRSAPPPCEIFVRAQFDYNPLDDELIPCAQAGISFQVGDILQIISKDDHHWWQARLDTVGGSAGLIPSPELQEWRIACQTVDKTKQEQVNCSIFGRKKKQCRDKYLAKHNAIFDTLDVVTYEEVVKVPVGDPNFQRKTLVLLGAHGVGRRHIKNTLISKYPDKYAYPIPHTTRPAKPEEENGRSYYFVSHDEMMADIGANEYLEYGTHEDAMYGTKLDTIRRIHTEGKMAILDVEPQALKILRTAEFTPYVVFIAAPSLQNIADYDGSLERLAKESEMLRQLYGHFFDLTIVNNDISETIATLETAIDRVHTTPQWVPVSWLY 5B16014EC66E0E9B kcnae_drome Potassium voltage-gated channel protein eag eag Ether-a-go-go protein CG10952 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MPGGRRGLVAPQNTFLENIIRRSNSQPDSSFLLANAQIVDFPIVYCNESFCKISGYNRAEVMQKSCRYVCGFMYGELTDKETVGRLEYTLENQQQDQFEILLYKKNNLQCGCALSQFGKAQTQETPLWLLLQVAPIRNERDLVVLFLLTFRDITALKQPIDSEDTKGVLGLSKFAKLARSVTRSRQFSAHLPTLKDPTKQSNLAHMMSLSADIMPQYRQEAPKTPPHILLHYCAFKAIWDWVILCLTFYTAIMVPYNVAFKNKTSEDVSLLVVDSIVDVIFFIDIVLNFHTTFVGPGGEVVSDPKVIRMNYLKSWFIIDLLSCLPYDVFNAFDRDEDGIGSLFSALKVVRLLRLGRVVRKLDRYLEYGAAMLILLLCFYMLVAHWLACIWYSIGRSDADNGIQYSWLWKLANVTQSPYSYIWSNDTGPELVNGPSRKSMYVTALYFTMTCMTSVGFGNVAAETDNEKVFTICMMIIAALLYATIFGHVTTIIQQMTSATAKYHDMLNNVREFMKLHEVPKALSERVMDYVVSTWAMTKGLDTEKVLNYCPKDMKADICVHLNRKVFNEHPAFRLASDGCLRALAMHFMMSHSAPGDLLYHTGESIDSLCFIVTGSLEVIQDDEVVAILGKGDVFGDQFWKDSAVGQSAANVRALTYCDLHAIKRDKLLEVLDFYSAFANSFARNLVLTYNLRHRLIFRKVADVKREKELAERRKNEPQLPQNQDHLVRKIFSKFRRTPQVQAGSKELVGGSGQSDVEKGDGEVERTKVFPKAPKLQASQATLARQDTIDEGGEVDSSPPSRDSRVVIEGAAVSSATVGPSPPVATTSSAAAGAGVSGGPGSGGTVVAIVTKADRNLALERERQIEMASSRATTSDTYDTGLRETPPTLAQRDLIATVLDMKVDVRLELQRMQQRIGRIEDLLGELVKRLAPGAGSGGNAPDNSSGQTTPGDEICAGCGAGGGGTPTTQAPPTSAVTSPVDTVITISSPGASGSGSGTGAGAGSAVAGAGGAGLLNPGATVVSSAGGNGLGPLMLKKRRSKSRKAPAPPKQTLASTAGTATAAPAGVAGSGMTSSAPASADQQQQHQSTADQSPTTPGAELLHLRLLEEDFTAAQLPSTSSGGAGGGGGSGSGATPTTPPPTTAGGSGSGTPTSTTATTTPTGSGTATRGKLDFL B8F86ACBB5627FD3 eag-cmg-1 2 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Immunoprecipitation reactions indicated that, in oocytes, EAG and CMG are part of the same protein complex. Fig. 2A Q02280, Q24210 transfection eag-cmg-2 2 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Immunoprecipitation reactions indicated that, in oocytes, EAG and CMG are part of the same protein complex. Fig. 2A Q02280, Q24210 transfection eag-cmg-3 4 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association EAG and CMG coimmunoprecipitate from Drosophila extracts Fig. 4 eag-cmg-4 3 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction A direct association of EAG and CMG was suggested by in vitro binding assays (Fig. 5). Fig. 5A The expression system for His-tagged proteins not given. Subsequent deletions suggested a shorter construct may bind but results were not consistent. eag-cmg-5 3 7 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sh3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain guk region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ck region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction A direct association of EAG and CMG was suggested by in vitro binding assays (Fig. 5). Fig. 5B The expression system for His-tagged proteins not given. eag-cmg-6 3 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined guk region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction A direct association of EAG and CMG was suggested by in vitro binding assays (Fig. 5). Fig. 5C The expression system for His-tagged proteins not given. eag-cmg-7 3 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sh3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 45 42 direct interaction direct interaction A direct association of EAG and CMG was suggested by in vitro binding assays (Fig. 5). Figs. 5C, 5D, 5F The expression system for His-tagged proteins not given. The interaction is resistant to increases in salt concentration, suggesting that binding is not solely mediated by electrostatic interactions. eag-cmg-8 3 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ck region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 49 51 direct interaction direct interaction A direct association of EAG and CMG was suggested by in vitro binding assays (Fig. 5). Fig. 5C The expression system for His-tagged proteins not given. dlg1-cmg-1 3 5 unspecified role unspecified role bait bait sh3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction A direct association of EAG and CMG was suggested by in vitro binding assays (Fig. 5). Figs. 5C, 5D The expression system for His-tagged proteins not given. eag-cmg-9 1 6 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sh3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction In ELISAs (Fig. 5E), full-lengthCMGbound 6H EAG869 1107 with a Kd of 1 2 nM, whereas the CMG SH3 domain displayed a Kd of 20 nM. Figs. 5C, 5D The expression system for His-tagged proteins not given. Full-length caki bound eag with a KD of 1-2nM eag-cmg-10 3 7 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q02280:p.Arg1037Leu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R L Wed May 02 16:03:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1037leu' Tue Jun 30 20:06:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1037Leu' Q02280:p.Lys1040Met mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K M Wed May 02 16:58:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys1040met' Tue Jun 30 16:19:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys1040Met' 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 6B The expression system for His-tagged proteins not given.