I2D I2D Protein Ser/Thr phosphatases PPEF interact with calmodulin. Protein Ser/Thr phosphatases PPEF interact with calmodulin. Biochem. Biophys. Res. Commun. (0006-291X) 2002 imex curation Kutuzov MA., Solov'eva OV., Andreeva AV., Bennett N. m.kutuzov@usa.net 21-Dec-2010: Contacted by JYOTI. Only protein-protein interactions Curated by: I2D - Interologous Interaction Database in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Kutuzov MA., Solov'eva OV., Andreeva AV., Bennett N. m.kutuzov@usa.net Accepted 2010-NOV-7 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Biochem. Biophys. Res. Commun. (0006-291X) 2002 orchard Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Protein Ser/Thr phosphatases PPEF interact with calmodulin. Protein Ser/Thr phosphatases PPEF interact with calmodulin. Biochem. Biophys. Res. Commun. (0006-291X) 2002 imex curation Kutuzov MA., Solov'eva OV., Andreeva AV., Bennett N. m.kutuzov@usa.net 21-Dec-2010: Contacted by JYOTI. Only protein-protein interactions Curated by: I2D - Interologous Interaction Database yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kutuzov MA., Solov'eva OV., Andreeva AV., Bennett N. m.kutuzov@usa.net Accepted 2010-NOV-7 by ORCHARD. 21-Dec-2010: Contacted by JYOTI. Biochem. Biophys. Res. Commun. (0006-291X) 2002 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database calm_mouse Calmodulin Calm1 Calm Cam1 Calm2 Cam2 Camb Calm3 Cam3 Camc Cam protein protein mouse Mus musculus Mouse MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK DIP protein P62204 original sequence version: 133 6B4BC3FCDE10727B ppe1_human Serine/threonine-protein phosphatase with EF-hands 1 PPEF1 PPEF PPP7C Protein phosphatase with EF calcium-binding domain Serine/threonine-protein phosphatase 7 protein protein human Homo sapiens Human MGCSSSSTKTRRSDTSLRAALIIQNWYRGYKARLKARQHYALTIFQSIEYADEQGQMQLSTFFSFMLENYTHIHKEELELRNQSLESEQDMRDRWDYVDSIDVPDSYNGPRLQFPLTCTDIDLLLEAFKEQQILHAHYVLEVLFETKKVLKQMPNFTHIQTSPSKEVTICGDLHGKLDDLFLIFYKNGLPSERNPYVFNGDFVDRGKNSIEILMILCVSFLVYPNDLHLNRGNHEDFMMNLRYGFTKEILHKYKLHGKRILQILEEFYAWLPIGTIVDNEILVIHGGISETTDLNLLHRVERNKMKSVLIPPTETNRDHDTDSKHNKVGVTFNAHGRIKTNGSPTEHLTEHEWEQIIDILWSDPRGKNGCFPNTCRGGGCYFGPDVTSKILNKYQLKMLIRSHECKPEGYEICHDGKVVTIFSASNYYEEGSNRGAYIKLCSGTTPRFFQYQVTKATCFQPLRQRVDTMENSAIKILRERVISRKSDLTRAFQLQDHRKSGKLSVSQWAFCMENILGLNLPWRSLSSNLVNIDQNGNVEYMSSFQNIRIEKPVQEAHSTLVETLYRYRSDLEIIFNAIDTDHSGLISVEEFRAMWKLFSSHYNVHIDDSQVNKLANIMDLNKDGSIDFNEFLKAFYVVHRYEDLMKPDVTNLG DF7B78C444EE6484 ppe2_human Serine/threonine-protein phosphatase with EF-hands 2 PPEF2 protein protein human Homo sapiens Human MGSGTSTQHHFAFQNAERAFKAAALIQRWYRRYVARLEMRRRCTWSIFQSIEYAGQQDQVKLHDFFSYLMDHFIPSSHNDRDFLTRIFTEDRFAQDSEMKKCSDYESIEVPDSYTGPRLSFPLLPDHATALVEAFRLKQQLHARYVLNLLYETKKHLVQLPNINRVSTCYSEEITVCGDLHGQLDDLIFIFYKNGLPSPERSYVFNGDFVDRGKDSVEILMILFAFMLVYPKEFHLNRGNHEDHMVNLRYGFTKEVMNKYKVHGKEILRTLQDVFCWLPLATLIDEKVLILHGGVSDITDLELLDKIERSKIVSTMRCKTRQKSEKQMEEKRRANQKSSAQGPIPWFLPESRSLPSSPLRLGSYKAQKTSRSSSIPCSGSLDGRELSRQVRSSVELELERCRQQAGLLVTGEKEEPSRSASEADSEAGELRKPTQEEWRQVVDILWSDPMAQEGCKANTIRGGGCYFGPDVTQQLLQKYNMQFLIRSHECKPEGYEFCHNRKVLTIFSASNYYEVGSNRGAYVKLGPALTPHIVQYQANKVTHTLTMRQRISRVEESALRALREKLFAHSSDLLSEFKKHDADKVGLITLSDWAAAVESVLHLGLPWRMLRPQLVNSSADNMLEYKSWLKNLAKEQLSRENIQSSLLETLYRNRSNLETIFRIIDSDHSGFISLDEFRQTWKLFSSHMNIDITDDCICDLARSIDFNKDGHIDINEFLEAFRLVEKSCPEGDASECPQATNAKDSGCSSPGAH 28ED6E79C5B8906C ppef1-calm 2 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table I ppef2-calm 2 3 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain physical association physical association Table I ppef1-calm-2 1 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal Dihydrofolate Reductase fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1a ppef2-calm-2 1 3 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal Dihydrofolate Reductase fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1b, 3 PPEF2exon2-DHFR binding to Calmodulin is dependent on Ca2+ concentration.