IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex. Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex. J. Mol. Biol. (0022-2836) 2001 imex curation Chen J., Rappsilber J., Chiang YC., Russell P., Mann M., Denis CL. This complex was further purified by gel filtration and anti-CCR4 coimmunoprecipitation. cldenis@christa.unh.edu 23-Feb-2010: Contacted by JYOTI. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Chen J., Rappsilber J., Chiang YC., Russell P., Mann M., Denis CL. This complex was further purified by gel filtration and anti-CCR4 coimmunoprecipitation. cldenis@christa.unh.edu Accepted 2010-MAR-10 by JYOTI. 23-Feb-2010: Contacted by JYOTI. J. Mol. Biol. (0022-2836) 2001 jyoti Only protein-protein interactions imex curation Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex. Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex. J. Mol. Biol. (0022-2836) 2001 imex curation Chen J., Rappsilber J., Chiang YC., Russell P., Mann M., Denis CL. This complex was further purified by gel filtration and anti-CCR4 coimmunoprecipitation. cldenis@christa.unh.edu 23-Feb-2010: Contacted by JYOTI. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Chen J., Rappsilber J., Chiang YC., Russell P., Mann M., Denis CL. Beta-galactosidase activity was measured in units/mg protein. This complex was further purified by gel filtration and anti-CCR4 coimmunoprecipitation. cldenis@christa.unh.edu Accepted 2010-MAR-10 by JYOTI. 23-Feb-2010: Contacted by JYOTI. J. Mol. Biol. (0022-2836) 2001 jyoti Only protein-protein interactions imex curation Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex. Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex. J. Mol. Biol. (0022-2836) 2001 imex curation Chen J., Rappsilber J., Chiang YC., Russell P., Mann M., Denis CL. This complex was further purified by gel filtration and anti-CCR4 coimmunoprecipitation. cldenis@christa.unh.edu 23-Feb-2010: Contacted by JYOTI. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Chen J., Rappsilber J., Chiang YC., Russell P., Mann M., Denis CL. This complex was further purified by gel filtration and anti-CCR4 coimmunoprecipitation. cldenis@christa.unh.edu Accepted 2010-MAR-10 by JYOTI. 23-Feb-2010: Contacted by JYOTI. J. Mol. Biol. (0022-2836) 2001 jyoti Only protein-protein interactions imex curation Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex. Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex. J. Mol. Biol. (0022-2836) 2001 imex curation Chen J., Rappsilber J., Chiang YC., Russell P., Mann M., Denis CL. This complex was further purified by gel filtration and anti-CCR4 coimmunoprecipitation. cldenis@christa.unh.edu 23-Feb-2010: Contacted by JYOTI. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down fingerprinting peptide massfingerprinting fingerprinting Chen J., Rappsilber J., Chiang YC., Russell P., Mann M., Denis CL. This complex was further purified by gel filtration and anti-CCR4 coimmunoprecipitation. cldenis@christa.unh.edu Accepted 2010-MAR-10 by JYOTI. 23-Feb-2010: Contacted by JYOTI. J. Mol. Biol. (0022-2836) 2001 jyoti Only protein-protein interactions imex curation Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex. Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex. J. Mol. Biol. (0022-2836) 2001 imex curation Chen J., Rappsilber J., Chiang YC., Russell P., Mann M., Denis CL. This complex was further purified by gel filtration and anti-CCR4 coimmunoprecipitation. cldenis@christa.unh.edu 23-Feb-2010: Contacted by JYOTI. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chen J., Rappsilber J., Chiang YC., Russell P., Mann M., Denis CL. This complex was further purified by gel filtration and anti-CCR4 coimmunoprecipitation. cldenis@christa.unh.edu Accepted 2010-MAR-10 by JYOTI. 23-Feb-2010: Contacted by JYOTI. J. Mol. Biol. (0022-2836) 2001 jyoti Only protein-protein interactions imex curation not2_yeast General negative regulator of transcription subunit 2 CDC36 DNA19 NOT2 YDL165W cell division cycle protein 36 protein protein yeast Saccharomyces cerevisiae Baker's yeast MEKFGLKALVPLLKLEDKELSSTYDHSMTLGADLSSMLYSLGIPRDSQDHRVLDTFQSPWAETSRSEVEPRFFTPESFTNIPGVLQSTVTPPCFNSIQNDQQRVALFQDETLFFLFYKHPGTVIQELTYLELRKRNWRYHKTLKAWLTKDPMMEPIVSADGLSERGSYVFFDPQRWEKCQRDFLLFYNAIM 7D0B69657F791371 7D0B69657F791371 ssn2_schpo Mediator of RNA polymerase II transcription subunit 13 srb9 med13 trap240 Mediator complex subunit 13 Suppressor of RNA polymerase B srb9 SPAC589.02c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSYQVCFKKYVKMRQWPHVLSLRVYASYFLLYNNLPLSSAMVHDGVHLISIKTILTDQPCPNTIYYAKYQVQRKDNEDAASLLEDKEAYLRNQDCIVHAKNDLLFVYDFQAIPSIPEESSSFMLLNSGAFSRLALFQKDELALLLDLYINFLQGLKKTVLYWLCKEYNFVPIYGVLLPLKLHPSFDTQLWNIYGYPVVDLQLSVLSKGHIEFYLKPTRQTVYRLSEVDNLVKKLDTVIRLAPTGCLATLTSVHANASAQTVDALKHRYGFSLTTTSKWVGVTLESSALEFSWPLELCFLETSALRMNDDSLSSNLTDLNNLVLPSNVVNNKKELTEFANEEAEASDKRKEGFTEKEETADAVVTLVPSHSSSPVNYSINSAKSTPASIKVNEEILVADHNVSDDILMEEIDDVGITEADFDYFDLPNVEEKVEMIEPNFANTMTTLDNEEINTSISQSNTSPNLNTHENIPKQMEIQSDDRMVTEDLNPYNVEVDIPEISLNISDSKIPTSAYMPSYYSAVIFPSSISSIFQKYNYGGKYWCPSPSLSTEDLFESFSVAESVTSTDEDICSTNFIQQDFTMEYNHDFFSSSKTPTNISEQSNPDSNYDTLSLAHQVLMNESKSANFDFSFLKSLDLQPTITLGKNDLLNAILSQNLWFRSLPFWKSMTTSFMMSQDVLNFSSYMRKPIRDYLEKILLGESSAVFLSKSPENYLSSINNGHHALNDNPPSQVNFSETLVNFSQPPRVLLKYNEKKLSLDSSAPENWISLCLQPYGESKDFEVFLLSSKSPDVSSKAISFFYDVQLAYENCKLGKLNLSETSINERVMGFSTNINETDNYDDNETTQSDTATSYEQLASVCVNELSGKNVLFFYFLEDDSEKLLKACQHFICVKDSIKRLGDNKFEDKSLRICTIPNSIFDSPNSHTTNSNSFFTKVSLDIYNNDPLLMDGSLKRREPAFLLKKPLLSTLNYQLKDINPRSSALGEYALHVTYTTVEEHLLICNWNDSYGEFETERRYFLQDLEIEDALQQILEVTFTFLNSMHMDWIVIVMKIGEMSDAEYLFWDQAIIPENLQGNVSLTVGYCSAEHGPGSTSKVFSRIPYSASSTVIRNNSSHELSLVAFIREMAMPVPNDEFKKISTILARGYLALDEDESYLPLLSIHLLISRNHDPYLMLNLILKHYLSMIYLQFRTYVSFSSLPLHISTVLYQKQLLQFMASDITHPVTS 19C84096CEEB99BD not4_yeast General negative regulator of transcription subunit 4 MOT2 CCL1 NOT4 SIG1 SSF1 Modulator of transcription 2 YER068W protein protein yeast Saccharomyces cerevisiae Baker's yeast MMNPHVQENLQAIHNALSNFDTSFLSEDEEDYCPLCIEPMDITDKNFFPCPCGYQICQFCYNNIRQNPELNGRCPACRRKYDDENVRYVTLSPEELKMERAKLARKEKERKHREKERKENEYTNRKHLSGTRVIQKNLVYVVGINPPVPYEEVAPTLKSEKYFGQYGKINKIVVNRKTPHSNNTTSEHYHHHSPGYGVYITFGSKDDAARCIAQVDGTYMDGRLIKAAYGTTKYCSSYLRGLPCPNPNCMFLHEPGEEADSFNKRELHNKQQAQQQSGGTAFTRSGIHNNISTSTAGSNTNLLSENFTGTPSPAAMRAQLHHDSHTNAGTPVLTPAPVPAGSNPWGVTQSATPVTSINLSKNSSSINLPTLNDSLGHHTTPTTENTITSTTTTTNTNATSHSHGSKKKQSLAAEEYKDPYDALGNAVDFLDARLHSLSNYQKRPISIKSNIIDEETYKKYPSLFSWDKIEASKKSDNTLANKLVEILAIKPIDYTASVVQFLQSVNVGVNDNITITDNTKTPTQPIRLQTVSQQIQPPLNVSTPPPGIFGPQHKVPIQQQQMGDTSSRNSSDLLNQLINGRKIIAGN 8847084BD9BF48B7 8847084BD9BF48B7 dbf2_yeast Cell cycle protein kinase DBF2 DBF2 Dumbbell forming protein 2 YGR092W protein protein yeast Saccharomyces cerevisiae Baker's yeast MLSKSEKNVDLLAGNMSNLSFDGHGTPGGTGLFPNQNITKRRTRPAGINDSPSPVKPSFFPYEDTSNMDIDEVSQPDMDVSNSPKKLPPKFYERATSNKTQRVVSVCKMYFLEHYCDMFDYVISRRQRTKQVLEYLQQQSQLPNSDQIKLNEEWSSYLQREHQVLRKRRLKPKNRDFEMITQVGQGGYGQVYLARKKDTKEVCALKILNKKLLFKLNETKHVLTERDILTTTRSEWLVKLLYAFQDLQSLYLAMEFVPGGDFRTLLINTRCLKSGHARFYISEMFCAVNALHDLGYTHRDLKPENFLIDAKGHIKLTDFGLAAGTISNERIESMKIRLEKIKDLEFPAFTEKSIEDRRKMYNQLREKEINYANSMVGSPDYMALEVLEGKKYDFTVDYWSLGCMLFESLVGYTPFSGSSTNETYDNLRRWKQTLRRPRQSDGRAAFSDRTWDLITRLIADPINRLRSFEHVKRMSYFADINFSTLRSMIPPFTPQLDSETDAGYFDDFTSEADMAKYADVFKRQDKLTAMVDDSAVSSKLVGFTFRHRNGKQGSSGILFNGLEHSDPFSTFY 648C565D5CE7117D 648C565D5CE7117D not3_yeast General negative regulator of transcription subunit 3 NOT3 YIL038C protein protein yeast Saccharomyces cerevisiae Baker's yeast MAHRKLQQEVDRVFKKINEGLEIFNSYYERHESCTNNPSQKDKLESDLKREVKKLQRLREQIKSWQSSPDIKDKDSLLDYRRSVEIAMEKYKAVEKASKEKAYSNISLKKSETLDPQERERRDISEYLSQMIDELERQYDSLQVEIDKLLLLNKKKKTSSTTNDEKKEQYKRFQARYRWHQQQMELALRLLANEELDPQDVKNVQDDINYFVESNQDPDFVEDETIYDGLNLQSNEAIAHEVAQYFASQNAEDNNTSDANESLQDISKLSKKEQRKLEREAKKAAKLAAKNATGAAIPVAGPSSTPSPVIPVADASKETERSPSSSPIHNATKPEEAVKTSIKSPRSSADNLLPSLQKSPSSATPETPTNVHTHIHQTPNGITGATTLKPATLPAKPAGELKWAVAASQAVEKDRKVTSASSTISNTSTKTPTTAAATTTSSNANSRIGSALNTPKLSTSSLSLQPDNTGASSSAATAAAVLAAGAAAVHQNNQAFYRNMSSSHHPLVSLATNPKSEHEVATTVNQNGPENTTKKVMEQKEEESPEERNKLQVPTFGVFDDDFESDRDSETEPEEEEQPSTPKYLSLEQREAKTNEIKKEFVSDFETLLLPSGVQEFIMSSELYNSQIESKITYKRSRDMCEISRLVEVPQGVNPPSPLDAFRSTQQWDVMRCSLRDIIIGSERLKEDSSSIYAKILENFRTLEMFSLFYNYYFAITPLEREIAYKILNERDWKVSKDGTMWFLRQGEVKFFNEICEVGDYKIFKLDDWTVIDKINFRLDYSFLQPPVDTASEVRDVSVDNNNVNDQSNVTLEQQKQEISHGKQLLKQLKQGKISV 69B76694FCC6846F 69B76694FCC6846F cnot1_human CCR4-NOT transcription complex subunit 1 CNOT1 CDC39 KIAA1007 NOT1 Negative regulator of transcription subunit 1 homolog CCR4-associated factor 1 AD-005 protein protein human Homo sapiens Human MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECALLITKPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIVQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDPLAGMTSLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTGALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSTIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFMQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTMVTTSTTTTVAKTVTVTRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENIQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSIRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDINELKPGNLLKDKDRLKNLDEQLSAPKKDVKQPEELPPITTTTTSTTPATNTTCTATVPPQPQYSYHDINVYSLAGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSISTNLKNSFASALRTASPQQREMMDQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLSQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPTLAMNPQAQALRSLLEVVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERSVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRSNYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQSEFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNLQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS DIP protein A5YKK6 original sequence version: 44 937A0899537BA615 not1_yeast General negative regulator of transcription subunit 1 CDC39 NOT1 ROS1 YCR093W YCR93W YCR1151 Cell division cycle protein 39 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLSATYRDLNTASNLETSKEKQAAQIVIAQISLLFTTLNNDNFESVEREIRHILDRSSVDIYIKVWERLLTLSSRDILQAGKFLLQENLLHRLLLEFAKDLPKKSTDLIELLKERTFNNQEFQKQTGITLSLFIDLFDKSANKDIIESLDRSSQINDFKTIKMNHTNYLRNFFLQTTPETLESNLRDLLHSLEGESLNDLLALLLSEILSPGSQNLQNDPTRSWLTPPMVLDATNRGNVIARSISSLQANQINWNRVFNLMSTKYFLSAPLMPTTASLSCLFAALHDGPVIDEFFSCDWKVIFKLDLAIQLHKWSVQNGCFDLLNAEGTRKVSETIPNTKQSLLYLLSIASLNLELFLQREELSDGPMLAYFQECFFEDFNYAPEYLILALVKEMKRFVLLIENRTVIDEILITLLIQVHNKSPSSFKDVISTITDDSKIVDAAKIIINSDDAPIANFLKSLLDTGRLDTVINKLPFNEAFKILPCARQIGWEGFDTFLKTKVSPSNVDVVLESLEVQTKMTDTNTPFRSLKTFDLFAFHSLIEVLNKCPLDVLQLQRFESLEFSLLIAFPRLINFGFGHDEAILANGDIAGINNDIEKEMQNYLQKMYSGELAIKDVIELLRRLRDSDLPRDQEVFTCITHAVIAESTFFQDYPLDALATTSVLFGSMILFQLLRGFVLDVAFRIIMRFAKEPPESKMFKFAVQAIYAFRIRLAEYPQYCKDLLRDVPALKSQAQVYQSIVEAATLANAPKERSRPVQEMIPLKFFAVDEVSCQINQEGAPKDVVEKVLFVLNNVTLANLNNKVDELKKSLTPNYFSWFSTYLVTQRAKTEPNYHDLYSKVIVAMGSGLLHQFMVNVTLRQLFVLLSTKDEQAIDKKHLKNLASWLGCITLALNKPIKHKNIAFREMLIEAYKENRLEIVVPFVTKILQRASESKIFKPPNPWTVGILKLLIELNEKANWKLSLTFEVEVLLKSFNLTTKSLKPSNFINTPEVIETLSGALGSITLEQQQTEQQRQIILMQQHQQQMLIYQQRQQQQQQRQQQQQHHISANTIADQQAAFGGEGSISHDNPFNNLLGSTIFVTHPDLKRVFQMALAKSVREILLEVVEKSSGIAVVTTTKIILKDFATEVDESKLKTAAIIMVRHLAQSLARATSIEPLKEGIRSTMQSLAPNLMSLSSSPAEELDTAINENIGIALVLIEKASMDKSTQDLADQLMQAIAIRRYHKERRADQPFITQNTNPYSLSLPEPLGLKNTGVTPQQFRVYEEFGKNIPNLDVIPFAGLPAHAPPMTQNVGLTQPQQQQAQMPTQILTSEQIRAQQQQQQLQKSRLNQPSQSAQPPGVNVPNPQGGIAAVQSDLEQNQRVLVHLMDILVSQIKENATKNNLAELGDQNQIKTIIFQILTFIAKSAQKDQLALKVSQAVVNSLFATSESPLCREVLSLLLEKLCSLSLVARKDVVWWLVYALDSRKFNVPVIRSLLEVNLIDATELDNVLVTAMKNKMENSTEFAMKLIQNTVLSDDPILMRMDFIKTLEHLASSEDENVKKFIKEFEDTKIMPVRKGTKTTRTEKLYLVFTEWVKLLQRVENNDVITTVFIKQLVEKGVISDTDNLLTFVKSSLELSVSSFKESDPTDEVFIAIDALGSLIIKLLILQGFKDDTRRDYINAIFSVIVLVFAKDHSQEGTTFNERPYFRLFSNILYEWATIRTHNFVRISDSSTRQELIEFDSVFYNTFSGYLHALQPFAFPGFSFAWVTLLSHRMLLPIMLRLPNKIGWEKLMLLIIDLFKFLDQYTSKHAVSDAVSVVYKGTLRVILGISNDMPSFLIENHYELMNNLPPTYFQLKNVILSAIPKNMTVPNPYDVDLNMEDIPACKELPEVFFDPVIDLHSLKKPVDNYLRIPSNSLLRTILSAIYKDTYDIKKGVGYDFLSVDSKLIRAIVLHVGIEAGIEYKRTSSNAVFNTKSSYYTLLFNLIQNGSIEMKYQIILSIVEQLRYPNIHTYWFSFVLMNMFKSDEWNDQKLEVQEIILRNFLKRIIVNKPHTWGVSVFFTQLINNNDINLLDLPFVQSVPEIKLILQQLVKYSKKYTTSEQDDQSATINRRQTPLQSNA 5B2AE15BCF95E009 5B2AE15BCF95E009 not5_yeast General negative regulator of transcription subunit 5 NOT5 YP9499.27 YPR072W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQRKLQQDIDKLLKKVKEGIEDFDDIYEKFQSTDPSNSSHREKLESDLKREIKKLQKHRDQIKTWLSKEDVKDKQSVLMTNRRLIENGMERFKSVEKLMKTKQFSKEALTNPDIIKDPKELKKRDQVLFIHDCLDELQKQLEQYEAQENEEQTERHEFHIANLENILKKLQNNEMDPEPVEEFQDDIKYYVENNDDPDFIEYDTIYEDMGCEIQPSSSNNEAPKEGNNQTSLSSIRSSKKQERSPKKKAPQRDVSISDRATTPIAPGVESASQSISSTPTPVSTDTPLHTVKDDSIKFDNSTLGTPTTHVSMKKKESENDSEQQLNFPPDRTDEIRKTIQHDVETNAAFQNPLFNDELKYWLDSKRYLMQPLQEMSPKMVSQLESSLLNCPDSLDADSPCLYTKPLSLPHPTSIFFPNEPIRFVYPYDVPLNLTNNENDTDNKFGKDSKAKSKKDDDIYSRTSLARIFMKFDLDTLFFIFYHYQGSYEQFLAARELFKNRNWLFNKVDRCWYYKEIEKLPPGMGKSEEESWRYFDYKKSWLARRCGNDFVYNEEDFEKL 073137119B3E92BC 073137119B3E92BC pop2_yeast Poly(A) ribonuclease POP2 POP2 CAF1 YNR052C N3470 CCR4-associated factor 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MQSMNVQPRVLAVGGEQFFSQRQASEQHQQQNMGPQVYSPKVNRARMFPQGMPVNTINGSVNQEMNNAYLLKQKNEPLLTQQQQQQQQQQQPFNIGTPVSVASLPPGLNVLQQQQQQQQQQQQQQQGVGLNRPLASQLPKHLTNQSMPPIFLPPPNYLFVRDVWKSNLYSEFAVIRQLVSQYNHVSISTEFVGTLARPIGTFRSKVDYHYQTMRANVDFLNPIQLGLSLSDANGNKPDNGPSTWQFNFEFDPKKEIMSTESLELLRKSGINFEKHENLGIDVFEFSQLLMDSGLMMDDSVTWITYHAAYDLGFLINILMNDSMPNNKEDFEWWVHQYMPNFYDLNLVYKIIQEFKNPQLQQSSQQQQQQQYSLTTLADELGLPRFSIFTTTGGQSLLMLLSFCQLSKLSMHKFPNGTDFAKYQGVIYGIDGDQ F3CAF97106A65933 F3CAF97106A65933 cf130_yeast Protein CAF130 CAF130 130 kDa CCR4-associated factor YGR134W protein protein yeast Saccharomyces cerevisiae Baker's yeast MTKKKAATNYAERQNLASEDSSGDSVHFKDFIPLQELLKDKNYVPSVENLEKILYNETMFNDQKICSNLLLEALIITLFTTISGKSALRLIQTSSLKERKSWAQSFENNSSSYASIVLSWKDNDILLLKFLRFLLANKTAPLQINRYNLPEYKLPLSFLIVSKITIPSILLNETYNLLKDYLYSITGRIESLISCSSTFDKPALVVRKILKDYNRMIECRNFYFWYSFNAENRVNLTFSDNISLLMENDEGNAGSGLDDSRFDHQKQPREAIMGRTINDQEQIYSFELNQDGTLEIPNVMEHSLLRHELLFKILNLTTVLTPLLELQFSTLCGLVDPLMQPTPNDKHIISIDFLFQLFLGLMSQSIKTSQEHNDHYDWKFYMCFNMQKIIDATMLRLNCFDFDILNSVNNTDNAVHWKTQLHRWLPHGLNTQDLELLYMIDILAVYTIYKLYEKIPIQLNPFLFSLISLWKNLSCVILLALEIDRIEEENGTYETPLMVRATIRGAAALRSVIATVLNGLVKNNDHDFKHESLNTFMSPYGRKLCHGALYADLRSHTASLLALGASIEDVTDLFADLQSGDRFDEDIRYMFDYECEDYDESFSESDHGGLDESVVNPTEKIASGSNNVFFRRRCNCIFNDDKLVAEDGANEAFGSTNSENVEGAMHNNRNAVHNATTATSDHVVTSPNPLSVRSRSTFEFDYSGEDWRDVPRDFNMYYSPSYSFIHEPKLDVIFSLTLRGATEKLNKEESILLVRSVASCVRNEQDQMILADLESNFSASINGDVEGEGNTKMSKIDNEDLRRTTPDDIYEIWSEESAFERMLNVNHDVAWRLMDEMLMCTGYRRILIWFLTHLELKHSLIYYVFELIMGLRGKPFSGEASDQDKKDDMIYEILKKKQKNEDASGLPFSRQGPIVLSDIETKMLLQEFFMNAAIFLSSKNNEEENEDGEKISLYSLGLVRLICYMVQTLIANDKFFFTKSECTFELQTLLMTWIGILPEAKDLFFKIKTRLAMEEEDSADTMQHEGRKNSDIEKKLNAKPASELNLKLLNLFPSKPANKDDSSPINTLRSFIADYSFDTQVNPPGRRVVFYDGKILPLPKADKPIPLHEYITLAELDVGDSE BF3542816CC78490 BF3542816CC78490 ccr4_yeast CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4 CCR4 Carbon catabolite repressor protein 4 Cytoplasmic deadenylase Glucose-repressible alcohol dehydrogenase transcriptional effector FUN27 YAL021C protein protein yeast Saccharomyces cerevisiae Baker's yeast MNDPSLLGYPNVGPQQQQQQQQQQHAGLLGKGTPNALQQQLHMNQLTGIPPPGLMNNSDVHTSSNNNSRQLLDQLANGNANMLNMNMDNNNNNNNNNNNNNNNGGGSGVMMNASTAAVNSIGMVPTVGTPVNINVNASNPLLHPHLDDPSLLNNPIWKLQLHLAAVSAQSLGQPNIYARQNAMKKYLATQQAQQAQQQAQQQAQQQVPGPFGPGPQAAPPALQPTDFQQSHIAEASKSLVDCTKQALMEMADTLTDSKTAKKQQPTGDSTPSGTATNSAVSTPLTPKIELFANGKDEANQALLQHKKLSQYSIDEDDDIENRMVMPKDSKYDDQLWHALDLSNLQIFNISANIFKYDFLTRLYLNGNSLTELPAEIKNLSNLRVLDLSHNRLTSLPAELGSCFQLKYFYFFDNMVTTLPWEFGNLCNLQFLGVEGNPLEKQFLKILTEKSVTGLIFYLRDNRPEIPLPHERRFIEINTDGEPQREYDSLQQSTEHLATDLAKRTFTVLSYNTLCQHYATPKMYRYTPSWALSWDYRRNKLKEQILSYDSDLLCLQEVESKTFEEYWVPLLDKHGYTGIFHAKARAKTMHSKDSKKVDGCCIFFKRDQFKLITKDAMDFSGAWMKHKKFQRTEDYLNRAMNKDNVALFLKLQHIPSGDTIWAVTTHLHWDPKFNDVKTFQVGVLLDHLETLLKEETSHNFRQDIKKFPVLICGDFNSYINSAVYELINTGRVQIHQEGNGRDFGYMSEKNFSHNLALKSSYNCIGELPFTNFTPSFTDVIDYIWFSTHALRVRGLLGEVDPEYVSKFIGFPNDKFPSDHIPLLARFEFMKTNTGSKKV 5CDB2E8FEDDBEF6F 5CDB2E8FEDDBEF6F caf40_yeast Protein CAF40 YNL288W N0540 40 kDa CCR4-associated factor CAF40 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFSAQKPIYGNGAGVNMGGGGPSTNNPGSMSMPGVPTSMGPGMNQQIPSGGPMLMGNTPNNNNSNENGENNGNNGNNGGNDANATRNNPNMVNNRGAVHALDDPNVYHWICQLTYGPQKEQALLELGRKREQFDDLAVVLWSSFGVMTSLLNEIISVYPMLQPQMLSNNLSNRVCNALVLLQCVASHPETKHLFLQAHIPLFLFPFLNTTSRQRTFEYLRLTSLGVIGALVKNDSQDVITFLLRTDIVPLCLRIMESSSELSKTVAIFILQKILLDDVGLQYICATLERFYAVTNVLKDMVEHLTVSTPPGRLLKHIIRCYLRLSDDLEARRLLKIVLPAKLRDNTFTEVLRDDVGSKRCLAQLLLTLNEETS F3DBABE6AD3C7F3C F3DBABE6AD3C7F3C pop2-not1-1 4 17 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association Fig. 1 ccr4-not1-2 5 15 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait 17 unspecified role unspecified role prey prey association association Fig. 2A As shown in Figure 2(a), both CAF130 and CAF40 could co-immunoprecipitate with CCR4, pop2-not1-2 5 8 unspecified role unspecified role prey prey 17 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey association association Fig. 2b ccr4-not3-1 1 15 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 17 unspecified role unspecified role bait bait physical association physical association Fig. 3 pop2-not1-3 5 15 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey 17 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey association association Fig. 4A, Fig. 5 CAF40 and CAF130 associate with NOT1 independently of CAF1 and CCR4. caf40 and caf130 deletions do not affect the formation of the CCR4-NOT complex. pop2-not1-4 5 17 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey association association Fig. 4B CAF40 and CAF130 associate with NOT1 independently of CAF1 and CCR4. pop2-not1-5 5 12 unspecified role unspecified role prey prey 17 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey association association Fig. 4B CAF40 and CAF130 associate with NOT1 independently of CAF1 and CCR4. Interactions observed in a pop2 -/- cell line. not1-caf40-1 2 12 unspecified role unspecified role bait bait 17 unspecified role unspecified role prey prey physical association physical association author score author-based confidence author score 44 Table 1 not1-caf130-1 2 15 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association author score author-based confidence author score 82 Table 1 not1-caf40-2 2 17 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association author score author-based confidence author score 700 Table 1 dbf2-caf40-1 2 9 unspecified role unspecified role prey prey 17 unspecified role unspecified role bait bait physical association physical association author score author-based confidence author score 940 Table 1 srb9-caf40-1 2 17 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association author score author-based confidence author score 1000 Table 1 not4-caf40-1 2 8 unspecified role unspecified role prey prey 17 unspecified role unspecified role bait bait physical association physical association author score author-based confidence author score 820 Table 1 not1-caf130-2 2 12 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role bait bait physical association physical association author score author-based confidence author score NOT1(1-667) 1500, NOT1(1152-2108) 1900 Table 1 not4-caf130-1 2 13 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait physical association physical association author score author-based confidence author score 29 Table 1 not1-caf130-3 3 12 unspecified role unspecified role prey prey region B42 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role bait bait region Lex A tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 6 Immunoprecipitation with anti-LexA antibody confirmed that LexA-CAF130 could co-immunoprecipitate with B42-NOT1(1-667) ( Figure 6, lane 7), B42-NOT1(1-1152) (lane 6), B42-NOT1(1152-2108) (lane 9) and B42-NOT1(1-2109) (lane 8).