MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Tipin/Tim1/And1 protein complex promotes Pol alpha chromatin binding and sister chromatid cohesion. Tipin/Tim1/And1 protein complex promotes Pol alpha chromatin binding and sister chromatid cohesion. EMBO J. (0261-4189) 2009 imex curation Errico A., Cosentino C., Rivera T., Losada A., Schwob E., Hunt T., Costanzo V. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Errico A., Cosentino C., Rivera T., Losada A., Schwob E., Hunt T., Costanzo V. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation Tipin/Tim1/And1 protein complex promotes Pol alpha chromatin binding and sister chromatid cohesion. Tipin/Tim1/And1 protein complex promotes Pol alpha chromatin binding and sister chromatid cohesion. EMBO J. (0261-4189) 2009 imex curation Errico A., Cosentino C., Rivera T., Losada A., Schwob E., Hunt T., Costanzo V. Only protein-protein interactions xenla- Xenopus laevis egg egg coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip predetermined predetermined participant predetermined Errico A., Cosentino C., Rivera T., Losada A., Schwob E., Hunt T., Costanzo V. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation dpola_xenla DNA polymerase alpha catalytic subunit pola1 pola DNA polymerase alpha catalytic subunit p180 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSDSGSFAASRSRREKTEKSGRKEALERLKRAKAGEKVKYEVEQVSSIYEEVDEAEYSKLVRDRQDDDWIVDDDGTGYVEDGREIFDDDLEDNALADSGKGAKGAPKDKTNVKKSSVSKPNNIKSMFMASAVKKTTDKAVDLSKDDLLGDLLQDLKSQAVPITPPPVITLKKKKLAGSPLNPFSVPPTAPKVLPTSVKRLPAVTKPGHPAAQSKASVPRQIKKEPKAELISSAVGPLKVEAQVKEEDSGMVEFDDGDFDEPMEEDVEITPVDSSTIKTQAQSIKCVKEENIKEEKSSFITSATLNESCWDQIDEAEPMTTEIQVDSSHLPLVTGADGSQVFRFYWLDAYEDQYSQPGVVYLFGKVWIESADAYVSCCVSVKNIERTVYLLPRENRVQLSTGKDTGAPVSMMHVYQEFNEAVAEKYKIMKFKSKKVDKDYAFEIPDVPASSEYLEVRYSADSPQLPQDLKGETFSHVFGTNTSSLELFLLSRKIKGPSWLEIKSPQLSSQPMSWCKVEAVVTRPDQVSVVKDLAPPPVVVLSLSMKTVQNAKTHQNEIVAIAALVHHTFPLDKAPPQPPFQTHFCVLSKLNDCIFPYDYNEAVKQKNANIEIALTERTLLGFFLAKIHKIDPDVIVGHDIYGFDLEVLLQRINSCKVPFWSKIGRLRRSVMPKLGGRSGFAERNAACGRIICDIEISAKELIRCKSYHLSELVHQILKAERVVIPPENIRNAYNDSVHLLYMLENTWIDAKFILQIMCELNVLPLALQITNIAGNVMSRTLMGGRSERNEYLLLHAFTENNFIVPDKPVFKKMQQTTVEDNDDMGTDQNKNKSRKKAAYAGGLVLEPKVGFYDKFILLLDFNSLYPSIIQEYNICFTTVHREAPSTQKGEDQDEIPELPHSDLEMGILPREIRKLVERRRHVKQLMKQPDLNPDLYLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTHQGREILLHTKEMVQKMNLEVIYGDTDSIMINTNCNNLEEVFKLGNRVKSEINKSYKLLEIDIDGIFKSLLLLKKKKYAALTVEPTGDGKYVTKQELKGLDIVRRDWCELAKQAGNYVISQILSDQPRDSIVENIQKKLTEIGENVTNGTVPITQYEINKALTKDPQDYPDKKSLPHVHVALWINSQGGRKVKAGDTISYVICQDGSNLSASQRAYAQEQLQKQENLSIDTQYYLSQQVHPVVARICEPIDGIDSALIAMWLGLDPSQFRAHRHYQQDEENDALLGGPSQLTDEEKYRDCERFKFFCPKCGTENIYDNVFDGSGLQIEPGLKRCSKPECDASPLDYVIQVHNKLLLDIRRYIKKYYSGWLVCEEKTCQNRTRRLPLSFSRNGPICQACSKATLRSEYPEKALYTQLCFYRFIFDWDYALEKVVSEQERGHLKKKLFQESENQYKKLKSTVDQVLSRSGYSEVNLSKLFQTLNTIK 25C29BAC5CC6B9E8 wdhd1_xenla WD repeat and HMG-box DNA-binding protein 1 Acidic nucleoplasmic DNA-binding protein 1 wdhd1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MPAIKKNMRYGHPEGHTDVCFDDSGNFLVTCGSDGDIRIWESLDDDDPKSISIGEKAYSFALKNGKVVTAASNNAIQLHTFPDGEPDGILTRFTTNANHVVFNTDGTRIAAGSGDFLVKVLQVEDSTQQKTLRGHSAPVLSVSFDPKDIYLASASCDGSVRIWKISDQTCEAVLPLLEKCNDVFNAKSICRLAWQPKSGKFVAIPVGKAVHLYDRDSLKNICTLSDDFITQPVNIVAWSPCGQYLVAGSVDGCIVAWNIATKACLERIKHEKGYTICALAWHPHLPQIAYTDNEGNLGLLEDVCQGDVKQPSAKVSSAETKDYDELFDGDDDEDFLNGDMIGHEGAVNDEDDDDNFTALTGRPRNRGAIFDDDISSDVPSLKLVGNENPVVEDDQASSVQNFTSVASVKLSYNGPMPTPQQKPFQSGSTPVHLMHRFMVWNSVGVIRCYNDEQDNAIDVEFHDTSIHHAIHLTNSLNHTLADVSQEAVLLACETTEELASKLQCLHFSSWDTSKEWMVDMPKGENIQAICLGQGWVACATSALLIRIFSVGGVQKELISLFGPVVCMASHGEQLIVVYHRGMGFDGDQCLGVQLLELGKKKKQVLHGDPLPLSRKSYLSWLGFTAEGSPCYVDSEGIVRLLNRSLGDTWVPICNTREHCKGKSDHYWVVGIHENPQQVRCIPCKGSRFPPTLPRPAVAVLPFNLPYCQITTEKGQMEEQYWRSQIFSNHSDYLSKHGYECDENFKAEAQKMQQELLMKMFALSCKLEREFRCMELAEFMTQNVMNLAIKYASRSKRLILAQRLSEMALEKAAEQASAEQNEEEDEEEEDFRSRLTAGYSRTATEWGDSRAKPVKQDQYEENNEEEMEEEEKEQEEALESNTPTANPFNKSVKTPDVSESKLGAILSSNQGRVNPFKVSASQKSPAFASNSSRSTSILDNMGKFPRKPSASGSPSTSKSDSVIIKPLAPKAKSKQGQATLFHSVQAKPIAKKTTEEKKAVPTPPKAAADIAENKKPKTGFQLWLDENRPSILSENAGLDESEIIKEGMSRFRMLTSEERMLWTEKAKGDYPGEDGADAKKRKRPEQENMASNGCPQENTDSGIAAAKKHKPLGQSANNKLSAFAFKKE 4F4C4A3FC7422082 tipin_xenla TIMELESS-interacting protein tipin XTipin protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MMDPLDNGLFDLPDYEHTEDENFPPLPPPHSPGADDEAEDVANGDDWTENAGQTQREEAPKPARRVVKRPQPKLDGQRLASQRGLPALRHMFDDVKFKGKGHETEDLKILLRQMENWAHRLFPKLQFEDFLNRLESMGNKKEVQTCLKKIRMDLPIVHDDFLSEEVVVQTEDHAIDMPSEDFSFPDELHVPSPSQPVKVDLSEETLQRIERNRRLALERRMEKMQAQAESQALSQATQSDPNEIPDDAFDAEMLDAVESMTGIPTASQSPPHVDKDLSLVLHSHDLPVQDVSQSPAPCPKPQGDAPPEKALSLVHSALPSTSDPTSPRKPLTEQLPDDADTSSATPYTEAPACTNTKEEY DIP protein Q0IHI4 original sequence version: 14 mint 7F839CB9B51032D8 tipin-pola1 2 tipin 5 polyclonal western polyclonal antibody western blot polyclonal western predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level pola (p180) 3 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f2a mint tipin-wdhd1 2 Tipin 5 polyclonal western polyclonal antibody western blot polyclonal western predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level and1 4 monoclonal western monoclonal antibody western blot monoclonal western predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f4a mint wdhd1-tipin 2 Tipin 5 polyclonal western polyclonal antibody western blot polyclonal western predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level and1 4 monoclonal western monoclonal antibody western blot monoclonal western predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f4a mint wdhd1-pola1 2 tipin 5 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell pola (p180) 3 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell and1 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell association association mint Immunoprecipitation experiments using Tipin or And1 antibodies on nuclear fraction (soluble chromatin proteins) in minimum or maximum licensing condition. fs2 tipin-pola1-1 2 tipin 5 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell pola (p180) 3 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell and1 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell association association mint Immunoprecipitation experiments using Tipin or And1 antibodies on nuclear fraction (soluble chromatin proteins) in minimum or maximum licensing condition. fs2 wdhd1-tipin-1 1 Tipin 5 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined and1 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified direct interaction direct interaction f4b mint wdhd1-pola1-1 1 and1 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pola (p180) 3 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra predetermined predetermined participant predetermined unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated direct interaction direct interaction f4c mint tipin-pola1-2 1 Tipin 5 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pola (p180) 3 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra predetermined predetermined participant predetermined unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated direct interaction direct interaction f4c mint