MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Analysis of the region for receptor binding and triggering of oligomerization on Bacillus thuringiensis Cry1Aa toxin. Analysis of the region for receptor binding and triggering of oligomerization on Bacillus thuringiensis Cry1Aa toxin. FEBS J. (1742-464X) 2009 imex curation Obata F., Kitami M., Inoue Y., Atsumi S., Yoshizawa Y., Sato R. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Obata F., Kitami M., Inoue Y., Atsumi S., Yoshizawa Y., Sato R. FEBS J. (1742-464X) 2009 Only protein-protein interactions imex curation q9xy09_bommo btr175 protein protein bommo Bombyx mori Silk moth MGVDVRILATLLLIYAETVLAQERCGFMVAIPRPPRPDLPELDFEGQTWSQRPLIPAADREDVCMDGYHAMTPTYGTQIIYMEEEIEGEVPIAKLNYRGPNVPYIEPAFLSGSFNLLVPVIRRIPDSNGEWHLIITQRQDYETPGMQQYVFNIRIDGETLVAGVSLLIVNIDDNAPIIQALEPCQVDELGEARLTECVYVVTDADGRISTQFMQFRIDSDRGDDKIFYIQGANIPGEWIRMTMTVGINEPLNFETNPLHIFSVTALDSLPNTHTVTLMVQVENVEHRPPRWVEIFAVQQFDEKTAQSFPVRAIDGDTGINKPIHYRLETAEEDTFFHIRTIEGGRSGAILYVDPIDRDTLQREVFQLSIIAYKYDNESSATAANVVIIVNDINDQRPEPLFKEYRLNIMEETALTLNFDQEFGFHDRDLGQNAQYTVRLESDYPADAAKAFYIAPEVGYQRQTFIMGTANHKMLDYEVPEFQRIRLRVIATDMDNEEHVGVAYVYINLINWNDEEPIFEHSVQNVSFKETEGKGFFVANVRAHDRDIDDRVEHTLMGNANNYLSIDKDTGDIHVTQDDFFDYHRQSELFVQVRADDTLGEPFHTATSQLLIHLEDINNTPPTLRLPRGSPNVEENVPEGYIITSEIRATDPDTTAELRFEIDWTTSYATKQGREANPIEFHNCVEIETIYPAINNRGSAIGRLVVKKIRENVTIDYEEFEMLYLTVRVRDLNTVIGDDYDESTFTITIIDMNDNPPIWVPGTLEQSLRVREMSDAGVVIGTLTATDIDGPLYNQVRYTMKANEGTPENLLMIDFYTGQITVKTSGAIDADVPRRYNLYYTVVATDRCYAEDPDDCPDDPTYWETPGQVVIQIIDTNNKIPQPETDQFKAVVYIYEDAVSGDEVVKVIGSDLDRDDIYHTIRYQINYAVNPRLRDFFAVDPDTGRVYVYYTTDEVLDRDGDEPQHRIFFNLIDNFFQQGDGNRNQNDAEVLVVLLDVNDNAPELPEPDELSWSVSESLTKGTRLQPHIYAPDRDEPDTDNSRVGYAIISLTIANREIEVPELFTMIQIQNVTGELETAMDLRGYWGTYAIHIKAYDHGIPQQMSNETYELVIRPYNFHAPVFVFPKHGATLRLARERAVVNGLLATVDGEFLNRIVATDEDGLHAGQVAFEVVGDTEAVDYFHIVNDGENSGTLMLKQLFPEDIREFEVTIRATDGGTEPRPLSTDCTFSVVFVPIQGEPIFPTSTHTVAFIEKEAGLLERHELPRAEDRKNHLCSDDCHNIYYRIIDGNNDGHFGLDETTNVLFLVKELDRSVSETYTLTIAASNSPTGGIALTSTITITVNVREADPQPYFVRDLYTAGISTSDSINRELLILQATHSENAPIIYTIDWSTMVTDPTLASVRETAFILNPHTGVLTLNIQPTASMHGMFEFQVVATDPAGYSDRANVKIYLISTRNRVFFLFVNTLEQVEQNTDFIAQTFSAGFEMTCNIDQVVPATDASGVIMNGITEVRGHFIRDNVPVPADEIETLRGDMVLLTAIQSTLATRLLVLRDLFTDTSPAPDAGSAAVLYALAVLSALLAALCLLLLVIFIIRTKKLNRRLEALTVKKYGSVDSGLNRVGIAAPGTNKHAVEGSNPIWNETIKAPDFDSMSDASNDSDLIGIEDLPHFGENNYFPRDVDEFKTDKPEDIVATHNNNFGFKSTPFSPEFANQFQK mint 2EC68401FB054C0F cr1aa_bactk Pesticidal crystal protein Cry1Aa cry1Aa cry-1-1 cry1A(a) cryA crybns3-1 cryIA(a) icp Insecticidal delta-endotoxin CryIA(a) Crystaline entomocidal protoxin 133 kDa crystal protein protein protein bactk Bacillus thuringiensis subsp. kurstaki MDNNPNINECIPYNCLSNPEVEVLGGERIETGYTPIDISLSLTQFLLSEFVPGAGFVLGLVDIIWGIFGPSQWDAFPVQIEQLINQRIEEFARNQAISRLEGLSNLYQIYAESFREWEADPTNPALREEMRIQFNDMNSALTTAIPLLAVQNYQVPLLSVYVQAANLHLSVLRDVSVFGQRWGFDAATINSRYNDLTRLIGNYTDYAVRWYNTGLERVWGPDSRDWVRYNQFRRELTLTVLDIVALFSNYDSRRYPIRTVSQLTREIYTNPVLENFDGSFRGMAQRIEQNIRQPHLMDILNSITIYTDVHRGFNYWSGHQITASPVGFSGPEFAFPLFGNAGNAAPPVLVSLTGLGIFRTLSSPLYRRIILGSGPNNQELFVLDGTEFSFASLTTNLPSTIYRQRGTVDSLDVIPPQDNSVPPRAGFSHRLSHVTMLSQAAGAVYTLRAPTFSWQHRSAEFNNIIPSSQITQIPLTKSTNLGSGTSVVKGPGFTGGDILRRTSPGQISTLRVNITAPLSQRYRVRIRYASTTNLQFHTSIDGRPINQGNFSATMSSGSNLQSGSFRTVGFTTPFNFSNGSSVFTLSAHVFNSGNEVYIDRIEFVPAEVTFEAEYDLERAQKAVNELFTSSNQIGLKTDVTDYHIDQVSNLVECLSDEFCLDEKQELSEKVKHAKRLSDERNLLQDPNFRGINRQLDRGWRGSTDITIQGGDDVFKENYVTLLGTFDECYPTYLYQKIDESKLKAYTRYQLRGYIEDSQDLEIYLIRYNAKHETVNVPGTGSLWPLSAQSPIGKCGEPNRCAPHLEWNPDLDCSCRDGEKCAHHSHHFSLDIDVGCTDLNEDLGVWVIFKIKTQDGHARLGNLEFLEEKPLVGEALARVKRAEKKWRDKREKLEWETNIVYKEAKESVDALFVNSQYDQLQADTNIAMIHAADKRVHSIREAYLPELSVIPGVNAAIFEELEGRIFTAFSLYDARNVIKNGDFNNGLSCWNVKGHVDVEEQNNQRSVLVVPEWEAEVSQEVRVCPGRGYILRVTAYKEGYGEGCVTIHEIENNTDELKFSNCVEEEIYPNNTVTCNDYTVNQEEYGGAYTSRNRGYNEAPSVPADYASVYEEKSYTDGRRENPCEFNRGYRDYTPLPVGYVTKELEYFPETDKVWIEIGETEGTFIVDSVELLLMEE mint E2EE15AF12E5DD85 btr175-cry1aa 1 btr175 2 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cry1Aa 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified P0A366:p.Tyr445Cys mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y C Tue Jul 12 10:17:36 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 17:07:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr445cys' Tue Jun 30 17:03:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr445Cys' direct interaction direct interaction f2 mint btr175-cry1aa-1 1 btr175 2 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cry1Aa 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified P0A366:p.Arg311Cys mutation decreasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R C Tue Jul 12 10:19:08 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 15:06:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg311cys' Tue Jun 30 15:59:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg311Cys' direct interaction direct interaction mint Increased binding was observed in the DF-bound form of R311C. f2 F4 btr175-cry1aa-2 1 btr175 2 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cry1Aa 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified P0A366:p.Ser373Cys mutation decreasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S C Tue Jul 12 10:19:28 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 17:17:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser373cys' Tue Jun 30 16:43:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser373Cys' direct interaction direct interaction mint f2 F4 S373C displayed decreased binding only in the DF-bound form and not in the IAF-modified form. btr175-cry1aa-3 1 cry1Aa 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified P0A366:p.Asn376Cys mutation decreasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N C Tue Jul 12 10:19:38 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 15:55:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn376cys' Tue Jun 30 15:39:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn376Cys' btr175 2 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction f2 mint btr175-cry1aa-4 1 cry1Aa 3 peptide synthesis peptide synthesis predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain btr175 2 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction f5b mint btr175-cry1aa-5 1 cry1Aa 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified P0A366:p.Arg281Cys mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R C Tue Jul 12 10:16:50 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 18:22:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg281cys' Tue Jun 30 15:36:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg281Cys' btr175 2 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction f4 mint For R281C, a decrease in binding was seen in the DF-bound form, but not in the IAF-modified form. btr175-cry1aa-6 1 cry1Aa 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified P0A366:p.Gln293Cys mutation decreasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q C Tue Jul 12 10:17:01 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 17:00:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln293cys' Tue Jun 30 17:42:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln293Cys' btr175 2 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction f4 mint Although even the DF-bound form of Q293C demonstrated high binding ability. btr175-cry1aa-7 1 cry1Aa 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified P0A366:p.Gly442Cys mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G C Tue Jul 12 10:55:06 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 15:55:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly442cys' Tue Jun 30 15:36:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly442Cys' btr175 2 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction f4 mint G442C showed reduction of binding in both DF-bound and IAF-modified forms. btr175-cry1aa-8 1 cry1Aa 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified P0A366:p.Arg311Cys mutation decreasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R C Tue Jul 12 10:17:16 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 14:52:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg311cys' Tue Jun 30 19:41:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg311Cys' btr175 2 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction f4 mint The IAF-modified form showed reduced binding capability. btr175-cry1aa-9 1 cry1Aa 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified P0A366:p.Asn376Cys mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N C Tue Jul 12 10:14:02 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 16:42:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn376cys' Tue Jun 30 19:27:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn376Cys' btr175 2 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction f4 mint N376C showed decreased binding in both DF-bound and IAF-modified forms.