MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Hsp90 is regulated by a switch point in the C-terminal domain. Hsp90 is regulated by a switch point in the C-terminal domain. EMBO Rep. (1469-221X) 2009 imex curation Retzlaff M., Stahl M., Eberl HC., Lagleder S., Beck J., Kessler H., Buchner J. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Retzlaff M., Stahl M., Eberl HC., Lagleder S., Beck J., Kessler H., Buchner J. EMBO Rep. (1469-221X) 2009 Only protein-protein interactions imex curation Hsp90 is regulated by a switch point in the C-terminal domain. Hsp90 is regulated by a switch point in the C-terminal domain. EMBO Rep. (1469-221X) 2009 imex curation Retzlaff M., Stahl M., Eberl HC., Lagleder S., Beck J., Kessler H., Buchner J. Only protein-protein interactions in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Retzlaff M., Stahl M., Eberl HC., Lagleder S., Beck J., Kessler H., Buchner J. EMBO Rep. (1469-221X) 2009 Only protein-protein interactions imex curation hsp82_yeast ATP-dependent molecular chaperone HSP82 HSP90 YPL240C HSP82 Heat shock protein Hsp90 heat-inducible isoform 82 kDa heat shock protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSLSDPKQLETEPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSGTKAFMEALSAGADVSMIGQFGVGFYSLFLVADRVQVISKSNDDEQYIWESNAGGSFTVTLDEVNERIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEFVAYPIQLVVTKEVEKEVPIPEEEKKDEEKKDEEKKDEDDKKPKLEEVDEEEEKKPKTKKVKEEVQEIEELNKTKPLWTRNPSDITQEEYNAFYKSISNDWEDPLYVKHFSVEGQLEFRAILFIPKRAPFDLFESKKKKNNIKLYVRRVFITDEAEDLIPEWLSFVKGVVDSEDLPLNLSREMLQQNKIMKVIRKNIVKKLIEAFNEIAEDSEQFEKFYSAFSKNIKLGVHEDTQNRAALAKLLRYNSTKSVDELTSLTDYVTRMPEHQKNIYYITGESLKAVEKSPFLDALKAKNFEVLFLTDPIDEYAFTQLKEFEGKTLVDITKDFELEETDEEKAEREKEIKEYEPLTKALKEILGDQVEKVVVSYKLLDAPAAIRTGQFGWSANMERIMKAQALRDSSMSSYMSSKKTFEISPKSPIIKELKKRVDEGGAQDKTVKDLTKLLYETALLTSGFSLDEPTSFASRINRLISLGLNIDEDEETETAPEASTAAPVEEVPADTEMEEVD D7C35676D668FB63 D7C35676D668FB63 hsp82-hsp82 2 Hsp90 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept purified purified fusion proteins fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined HSP90 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role fluorescence donor fluorescence donor purified purified tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction mint f3a st2 hsp82-1 1 Hsp90 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 direct interaction direct interaction t1 mint hsp82-1 1 Hsp90 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P02829:p.Ala577Ile mutation increasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain A I Tue Jul 12 10:16:34 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation increasing' Wed May 02 17:37:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala577ile' Tue Jun 30 20:06:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala577Ile' Stoichiometry: 2.0 direct interaction direct interaction t1 mint hsp82-1 1 Hsp90 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P02829:p.Ala577Asn mutation increasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain A N Tue Jul 12 10:16:32 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation increasing' Wed May 02 15:37:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala577asn' Tue Jun 30 16:23:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala577Asn' direct interaction direct interaction t1 mint