MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it A single intersubunit salt bridge affects oligomerization and catalytic activity in a bacterial quinone reductase. A single intersubunit salt bridge affects oligomerization and catalytic activity in a bacterial quinone reductase. FEBS J. (1742-464X) 2009 imex curation Binter A., Staunig N., Jelesarov I., Lohner K., Palfey BA., Deller S., Gruber K., Macheroux P. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Binter A., Staunig N., Jelesarov I., Lohner K., Palfey BA., Deller S., Gruber K., Macheroux P. FEBS J. (1742-464X) 2009 Only protein-protein interactions imex curation A single intersubunit salt bridge affects oligomerization and catalytic activity in a bacterial quinone reductase. A single intersubunit salt bridge affects oligomerization and catalytic activity in a bacterial quinone reductase. FEBS J. (1742-464X) 2009 imex curation Binter A., Staunig N., Jelesarov I., Lohner K., Palfey BA., Deller S., Gruber K., Macheroux P. Only protein-protein interactions in vitro In vitro bn-page blue native page bn-page weight by comassie molecular weight estimation by coomasie staining weight by comassie Binter A., Staunig N., Jelesarov I., Lohner K., Palfey BA., Deller S., Gruber K., Macheroux P. FEBS J. (1742-464X) 2009 Only protein-protein interactions imex curation azr_bacsu FMN-dependent NADPH-azoreductase azr yhdA Azobenzene reductase BSU09340 protein protein bacsu Bacillus subtilis MNMLVINGTPRKHGRTRIAASYIAALYHTDLIDLSEFVLPVFNGEAEQSELLKVQELKQRVTKADAIVLLSPEYHSGMSGALKNALDFLSSEQFKYKPVALLAVAGGGKGGINALNNMRTVMRGVYANVIPKQLVLDPVHIDVENATVAENIKESIKELVEELSMFAKAGNPGV mint A51E12FF6D9099D1 azr 1 YhdA 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 4.0 direct interaction direct interaction mint F4 F5 F6 F7 F8 The remaining two variants (K109L and D137L) show different oligomeric states in each case with 4 protomers in the asymmetric unit, which form two dimers identical to the dimers found in the other YhdA structures. In the crystal, these two dimers also form tetramers which according to the PISA analysis should only be marginally stable in solution (dGdiss of -0.1 and 1.4 kcal/mol). azr-1 2 YhdA 3 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 4.0 direct interaction direct interaction F3 mint azr-1 2 YhdA 3 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified O07529:p.Lys109Leu mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K L Tue Jul 12 10:23:04 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 17:29:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys109leu' Tue Jun 30 16:58:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys109Leu' Stoichiometry: 2.0 direct interaction direct interaction F3 mint azr-1 2 YhdA 3 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified O07529:p.Asp137Leu mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D L Tue Jul 12 10:58:21 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 18:13:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp137leu' Tue Jun 30 19:25:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp137Leu' Stoichiometry: 2.0 direct interaction direct interaction F3 mint azr-1 2 YhdA 3 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified O07529:p.Asp137Leu mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D L Tue Jul 12 10:58:23 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 16:10:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp137leu' Tue Jun 30 16:14:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp137Leu' O07529:p.Lys109Leu mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K L Tue Jul 12 10:23:00 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 18:27:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys109leu' Tue Jun 30 17:19:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys109Leu' Stoichiometry: 2.0 direct interaction direct interaction F3 mint azr-1 2 YhdA 3 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified O07529:p.Lys109Asp mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K D Tue Jul 12 10:15:23 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 15:01:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys109asp' Tue Jun 30 17:43:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys109Asp' O07529:p.Asp137Lys mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D K Tue Jul 12 10:54:10 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 16:47:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp137lys' Tue Jun 30 15:40:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp137Lys' direct interaction direct interaction F3 mint