IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ FRIGIDA delays flowering in Arabidopsis via a co-transcriptional mechanism involving direct interaction with the nuclear cap binding complex. FRIGIDA delays flowering in Arabidopsis via a co-transcriptional mechanism involving direct interaction with the nuclear cap binding complex. Plant Physiol. (0032-0889) 2009 imex curation Geraldo N., Baurle I., Kidou SI., Hu X., Dean C. caroline.dean@bbsrc.ac.uk. orchard Only protein-protein interactions nicbe Nicotiana benthamiana anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Geraldo N., Baurle I., Kidou SI., Hu X., Dean C. caroline.dean@bbsrc.ac.uk. Accepted 2009-AUG-14 by ORCHARD. Plant Physiol. (0032-0889) 2009 orchard Only protein-protein interactions imex curation FRIGIDA delays flowering in Arabidopsis via a co-transcriptional mechanism involving direct interaction with the nuclear cap binding complex. FRIGIDA delays flowering in Arabidopsis via a co-transcriptional mechanism involving direct interaction with the nuclear cap binding complex. Plant Physiol. (0032-0889) 2009 imex curation Geraldo N., Baurle I., Kidou SI., Hu X., Dean C. caroline.dean@bbsrc.ac.uk. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Geraldo N., Baurle I., Kidou SI., Hu X., Dean C. caroline.dean@bbsrc.ac.uk. Accepted 2009-AUG-14 by ORCHARD. Plant Physiol. (0032-0889) 2009 orchard Only protein-protein interactions imex curation rga_arath DELLA protein RGA RGA GRS RGA1 At2g01570 F2I9.19 Repressor on the ga1-3 mutant GAI-related sequence Restoration of growth on ammonia protein 1 GRAS family protein 10 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKRDHHQFQGRLSNHGTSSSSSSISKDKMMMVKKEEDGGGNMDDELLAVLGYKVRSSEMAEVALKLEQLETMMSNVQEDGLSHLATDTVHYNPSELYSWLDNMLSELNPPPLPASSNGLDPVLPSPEICGFPASDYDLKVIPGNAIYQFPAIDSSSSSNNQNKRLKSCSSPDSMVTSTSTGTQIGGVIGTTVTTTTTTTTAAGESTRSVILVDSQENGVRLVHALMACAEAIQQNNLTLAEALVKQIGCLAVSQAGAMRKVATYFAEALARRIYRLSPPQNQIDHCLSDTLQMHFYETCPYLKFAHFTANQAILEAFEGKKRVHVIDFSMNQGLQWPALMQALALREGGPPTFRLTGIGPPAPDNSDHLHEVGCKLAQLAEAIHVEFEYRGFVANSLADLDASMLELRPSDTEAVAVNSVFELHKLLGRPGGIEKVLGVVKQIKPVIFTVVEQESNHNGPVFLDRFTESLHYYSTLFDSLEGVPNSQDKVMSEVYLGKQICNLVACEGPDRVERHETLSQWGNRFGSSGLAPAHLGSNAFKQASMLLSVFNSGQGYRVEESNGCLMLGWHTRPLITTSAWKLSTAAY DIP protein Q9SLH3 original sequence version: 84 DIP protein Q9SLH3 original sequence version: 75 DIP protein Q9SLH3 original sequence version: 102 FC92E7F9408072AA frigi_arath Protein FRIGIDA F6N23.25 FRI At4g00650 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSNYPPTVAAQPTTTANPLLQRHQSEQRRRELPKIVETESTSMDITIGQSKQPQFLKSIDELAAFSVAVETFKRQFDDLQKHIESIENAIDSKLESNGVVLAARNNNFHQPMLSPPRNNVSVETTVTVSQPSQEIVPETSNKPEGGRMCELMCSKGLRKYIYANISDQAKLMEEIPSALKLAKEPAKFVLDCIGKFYLQGRRAFTKESPMSSARQVSLLILESFLLMPDRGKGKVKIESWIKDEAETAAVAWRKRLMTEGGLAAAEKMDARGLLLLVACFGVPSNFRSTDLLDLIRMSGSNEIAGALKRSQFLVPMVSGIVESSIKRGMHIEALEMVYTFGMEDKFSAALVLTSFLKMSKESFERAKRKAQSPLAFKEAATKQLAVLSSVMQCMETHKLDPAKELPGWQIKEQIVSLEKDTLQLDKEMEEKARSLSLMEEAALAKRMYNQQIKRPRLSPMEMPPVTSSSYSPIYRDRSFPSQRDDDQDEISALVSSYLGPSTSFPHRSRRSPEYMVPLPHGGLGRSVYAYEHLAPNSYSPGHGHRLHRQYSPSLVHGQRHPLQYSPPIHGQQQLPYGIQRVYRHSPSEERYLGLSNQRSPRSNSSLDPK FE8572DFF3E6726F ncbp2_arath Nuclear cap-binding protein subunit 2 CBP20 At5g44200 20 kDa nuclear cap-binding protein NCBP 20 kDa subunit MLN1.12 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASLFKEQAKLSAYRDRRFSGTQEEFDEALRASTTVYIGNVSFYTTEEQLYELFSRAGEIKKIIMGLDKNTKTPCGFCFVLFYSREDTEDAVKYISGTILDDRPIRVDFDWGFQEGRQWGRGRSGGQVRDEYRTDYDPARGGYGKLVQKELEAQRQLVDYGTGSLGAYPQAAPTNYGNGRRGGGNYGQGGQNRHGRGDYHRKRQRDDDRYGRDNSRRNTDHESRRDTDSDMRPEKNPRFRESGDSDDDGEDDRKRRS DD8B12A0639E1D58 frip1_arath Protein FIP1 FIP1 FRIGIDA-interacting protein 1 At2g06005 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSTERRASSNPMTNEENAMFLDILHEAPLFGHRKSRSLVGSYLYMLLLAGYAILAAGAPWMFHRVQQLTPSLLCCCDVALLVVTGVFQQYFVYQVQKIRLQGYYSFSQKLKHVVRLPFAIAAYGTAAMLLVIVWRPQIHILSISSLQRIIMLVEAVGAGFFMGLYIGYVHQYNSVNSRPDVLKSLYSPLQPSSSMEGLRYYEGRLSDQQTALLQYQRENLHFLSEEILCLQEKLSKYEQSDDGSTPQVDLAHLLAARDQELRTLSAEMNQLQSELRLARSLIAERDAEVQRVNSTNNQYIEENERLRAILSEWSMRAANLERALEVERMSNSELQKEVASTRRKQMLETTTSEQP 92EE836EE3F67AA2 q8gxh1_arath At4g17060/dl4560c protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTIPDDGSPVAMAIDSSTAVTVATTTTRRVPPPCWTDEETAALVNAYKDKWFALRRGNLRAADWDDVAAAVSSSSTVGGPPKSAIQCRHKIEKLRKRYRGEKQRALNRPGKFSSSWDLFPILDAMGFAPVTPAAVETYDPDVDHDDESNGLDGFRVRSKRSGKFSGGYSDSPREVGDGYGVRSRARSNMKMYGGFKSEFDSDHDSGSGFGLKRKYNGNPKVSADFDADSDDEIVLVPKATRLRTHGKPSSGDFSHGSGGGFPLKSFGDRNFASHGFKPKNFSKPEPNFSQDLDYDDEFDDDRAEREGFNPRIQSSRSSSRVNGYSRKDGSYPRNTGASNGYGSSSRFKHEQMNAAAEVESDPIDEVVSSVKMLTEMFVRVENSKMEMIREMEKSRMEMELKHCQMMLESQQQIIGAFAEALSEKKSTNARRPVS B21ED8C100B8F118 gid1b_arath Gibberellin receptor GID1B GID1B At3g63010 T20O10.110 GID1-like protein 2 CXE14 GID1L2 AtCXE14 Carboxylesterase 14 Protein GA INSENSITIVE DWARF 1B protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAGGNEVNLNECKRIVPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFDHVDSTTNLLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWVKSRVWLQSGKDSNVYVYLAGDSSGGNIAHNVAVRATNEGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQATIGFYFLPNNDHFHCLMEELNKFVHSIEDSQSKSSPVLLTP DIP protein Q9LYC1 original sequence version: 78 8D24F47BC40CE4D4 frl1a_arath FRIGIDA-like protein 1 FRL1 Protein SUPPRESSOR OF FRI 8 SUF8 MQK4.4 At5g16320 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTASETIATAINQIDEKKEKLKKAFDDLQAHRSLLSPSFSLSWSEIDSHFSSLQSSLASRFRLLHSTSPLEHDSYRIDASDAGKSSSSEEVSEQPVVEPELRALCEKIDGIGLIKYLIRIWDDETPLNQEVSAAIRYSPDTASMVLDAIEGSNYTPSSSGRSFDVRRVFVLLMEVLIEINANITVDTRNRAKKLAYHWKSKVGVKPFEALVFLHLVAAFELGSEFDTEELSDYVFMIAKYKQATLVCNKIGVDRKRVGKLIKTLLDSGKPILAVKFMYECGMTDEFEPIPVLKSYIKDCREAALRVCVEDNYSLKSQNEASDKEVSALKPLIKIIKDQNLESEFTQEKVEERVEELEKNKALRKRNTTNPPKQEPQQKGKKRTRDCKNGSQVPVPSQQLLSRPEALLMPEHSHHGLQLNPYGLMTSAFSGVVVNPLTGLFGSGATPQSLYYAQQTGYVLPPQYHPPYYSQ 2924997BB01366D6 fri-cbp20 2 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Supplementary Figure 3 fri-cbp20-1 1 5 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Figure 2 The interaction with CBP20 was confirmed using coimmunoprecipitation assays following Nicotiana benthamiana transient expression experiments using FRI-FLAG and CPP20-HA-tagged proteins (Fig. 2). fri-frl1 2 9 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Supplementary Figure 3 fri-fip1 2 4 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Supplementary Figure 3 fri-fip2 2 7 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Supplementary Figure 3 fri-fri 2 4 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Supplementary Figure 3 fri-fip1-1 2 4 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Supplementary Figure 3 fri-fip2-1 2 7 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association Supplementary Figure 3 fri-frl1-1 2 4 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association Supplementary Figure 3 gid1b-rga-1 1 8 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association Supplementary Figure 4 Gibberelic acid.