IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Disassembly of exon junction complexes by PYM. Disassembly of exon junction complexes by PYM. Cell (0092-8674) 2009 imex curation Gehring NH., Lamprinaki S., Kulozik AE., Hentze MW. It is not always clear if these experiments were performed as anti-bait or anti-tag coimmuniprecipitates - I have assumed that the same methodology is used throughout and is anti-bait coimmunoprecipitation. I have not tried to capture the RNA binding expts as I have no sequence for the RNA, or know anything about it. Cells were RNase treated. Species of origin of cDNAs taken from PMID: 12718880 and 16209946 as cited in the text. niels.gehring@med.uni.heidelberg.de, hentze@embl.de niels.gehring@med.uni.heidelberg.de davet Only protein-protein interactions 13 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Gehring NH., Lamprinaki S., Kulozik AE., Hentze MW. It is not always clear if these experiments were performed as anti-bait or anti-tag coimmuniprecipitates - I have assumed that the same methodology is used throughout and is anti-bait coimmunoprecipitation. I have not tried to capture the RNA binding expts as I have no sequence for the RNA, or know anything about it. Cells were RNase treated. Species of origin of cDNAs taken from PMID: 12718880 and 16209946 as cited in the text. niels.gehring@med.uni.heidelberg.de, hentze@embl.de niels.gehring@med.uni.heidelberg.de, hentze@embl.de Accepted 2009-AUG-23 by DAVET. Cell (0092-8674) 2009 davet Only protein-protein interactions 13 imex curation Disassembly of exon junction complexes by PYM. Disassembly of exon junction complexes by PYM. Cell (0092-8674) 2009 imex curation Gehring NH., Lamprinaki S., Kulozik AE., Hentze MW. It is not always clear if these experiments were performed as anti-bait or anti-tag coimmuniprecipitates - I have assumed that the same methodology is used throughout and is anti-bait coimmunoprecipitation. I have not tried to capture the RNA binding expts as I have no sequence for the RNA, or know anything about it. Cells were RNase treated. Species of origin of cDNAs taken from PMID: 12718880 and 16209946 as cited in the text. niels.gehring@med.uni.heidelberg.de, hentze@embl.de niels.gehring@med.uni.heidelberg.de davet Only protein-protein interactions 13 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gehring NH., Lamprinaki S., Kulozik AE., Hentze MW. It is not always clear if these experiments were performed as anti-bait or anti-tag coimmuniprecipitates - I have assumed that the same methodology is used throughout and is anti-bait coimmunoprecipitation. I have not tried to capture the RNA binding expts as I have no sequence for the RNA, or know anything about it. Cells were RNase treated. Species of origin of cDNAs taken from PMID: 12718880 and 16209946 as cited in the text. niels.gehring@med.uni.heidelberg.de, hentze@embl.de niels.gehring@med.uni.heidelberg.de, hentze@embl.de Accepted 2009-AUG-23 by DAVET. Cell (0092-8674) 2009 davet Only protein-protein interactions 13 imex curation Disassembly of exon junction complexes by PYM. Disassembly of exon junction complexes by PYM. Cell (0092-8674) 2009 imex curation Gehring NH., Lamprinaki S., Kulozik AE., Hentze MW. It is not always clear if these experiments were performed as anti-bait or anti-tag coimmuniprecipitates - I have assumed that the same methodology is used throughout and is anti-bait coimmunoprecipitation. I have not tried to capture the RNA binding expts as I have no sequence for the RNA, or know anything about it. Cells were RNase treated. Species of origin of cDNAs taken from PMID: 12718880 and 16209946 as cited in the text. niels.gehring@med.uni.heidelberg.de, hentze@embl.de niels.gehring@med.uni.heidelberg.de davet Only protein-protein interactions 13 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 cosedimentation cosedimentation western blot western blot Immuno blot Immuno blot Gehring NH., Lamprinaki S., Kulozik AE., Hentze MW. It is not always clear if these experiments were performed as anti-bait or anti-tag coimmuniprecipitates - I have assumed that the same methodology is used throughout and is anti-bait coimmunoprecipitation. I have not tried to capture the RNA binding expts as I have no sequence for the RNA, or know anything about it. Cells were RNase treated. Species of origin of cDNAs taken from PMID: 12718880 and 16209946 as cited in the text. niels.gehring@med.uni.heidelberg.de, hentze@embl.de niels.gehring@med.uni.heidelberg.de, hentze@embl.de Accepted 2009-AUG-23 by DAVET. Cell (0092-8674) 2009 davet Only protein-protein interactions 13 imex curation Disassembly of exon junction complexes by PYM. Disassembly of exon junction complexes by PYM. Cell (0092-8674) 2009 imex curation Gehring NH., Lamprinaki S., Kulozik AE., Hentze MW. It is not always clear if these experiments were performed as anti-bait or anti-tag coimmuniprecipitates - I have assumed that the same methodology is used throughout and is anti-bait coimmunoprecipitation. I have not tried to capture the RNA binding expts as I have no sequence for the RNA, or know anything about it. Cells were RNase treated. Species of origin of cDNAs taken from PMID: 12718880 and 16209946 as cited in the text. niels.gehring@med.uni.heidelberg.de, hentze@embl.de niels.gehring@med.uni.heidelberg.de davet Only protein-protein interactions 13 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Gehring NH., Lamprinaki S., Kulozik AE., Hentze MW. It is not always clear if these experiments were performed as anti-bait or anti-tag coimmuniprecipitates - I have assumed that the same methodology is used throughout and is anti-bait coimmunoprecipitation. I have not tried to capture the RNA binding expts as I have no sequence for the RNA, or know anything about it. Cells were RNase treated. Species of origin of cDNAs taken from PMID: 12718880 and 16209946 as cited in the text. niels.gehring@med.uni.heidelberg.de, hentze@embl.de niels.gehring@med.uni.heidelberg.de, hentze@embl.de Accepted 2009-AUG-23 by DAVET. Cell (0092-8674) 2009 davet Only protein-protein interactions 13 imex curation pym1_human Partner of Y14 and mago PYM1 PYM PYM homolog 1 exon junction complex-associated factor Protein wibg homolog WIBG protein protein human Homo sapiens Human MEAAGSPAATETGKYIASTQRPDGTWRKQRRVKEGYVPQEEVPVYENKYVKFFKSKPELPPGLSPEATAPVTPSRPEGGEPGLSKTAKRNLKRKEKRRQQQEKGEAEALSRTLDKVSLEETAQLPSAPQGSRAAPTAASDQPDSAATTEKAKKIKNLKKKLRQVEELQQRIQAGEVSQPSKEQLEKLARRRALEEELEDLELGL DIP protein Q9BRP8 original sequence version: 84 DIP protein Q9BRP8 original sequence version: 59 087B901279007C05 casc3_human Protein CASC3 CASC3 MLN51 Cancer susceptibility candidate gene 3 protein Metastatic lymph node gene 51 protein Protein barentsz protein protein human Homo sapiens Human MADRRRQRASQDTEDEESGASGSDSGGSPLRGGGSCSGSAGGGGSGSLPSQRGGRTGALHLRRVESGGAKSAEESECESEDGIEGDAVLSDYESAEDSEGEEGEYSEEENSKVELKSEANDAVNSSTKEEKGEEKPDTKSTVTGERQSGDGQESTEPVENKVGKKGPKHLDDDEDRKNPAYIPRKGLFFEHDLRGQTQEEEVRPKGRQRKLWKDEGRWEHDKFREDEQAPKSRQELIALYGYDIRSAHNPDDIKPRRIRKPRYGSPPQRDPNWNGERLNKSHRHQGLGGTLPPRTFINRNAAGTGRMSAPRNYSRSGGFKEGRAGFRPVEAGGQHGGRSGETVKHEISYRSRRLEQTSVRDPSPEADAPVLGSPEKEEAASEPPAAAPDAAPPPPDRPIEKKSYSRARRTRTKVGDAVKLAEEVPPPPEGLIPAPPVPETTPTPPTKTGTWEAPVDSSTSGLEQDVAQLNIAEQNWSPGQPSFLQPRELRGMPNHIHMGAGPPPQFNRMEEMGVQGGRAKRYSSQRQRPVPEPPAPPVHISIMEGHYYDPLQFQGPIYTHGDSPAPLPPQGMLVQPGMNLPHPGLHPHQTPAPLPNPGLYPPPVSMSPGQPPPQQLLAPTYFSAPGVMNFGNPSYPYAPGALPPPPPPHLYPNTQAPSQVYGGVTYYNPAQQQVQPKPSPPRRTPQPVTIKPPPPEVVSRGSS DIP protein O15234 original sequence version: 129 DIP protein O15234 original sequence version: 130 DIP protein O15234 original sequence version: 94 DIP protein O15234 original sequence version: 106 DIP protein O15234 original sequence version: 114 642A4C01C8DD3BE0 rbm8a_human RNA-binding protein 8A HSPC114 MDS014 RNA-binding motif protein 8A Ribonucleoprotein RBM8A RNA-binding protein Y14 Binder of OVCA1-1 RBM8A RBM8 protein protein human Homo sapiens Human MADVLDLHEAGGEDFAMDEDGDESIHKLKEKAKKRKGRGFGSEEGSRARMREDYDSVEQDGDEPGPQRSVEGWILFVTGVHEEATEEDIHDKFAEYGEIKNIHLNLDRRTGYLKGYTLVEYETYKEAQAAMEGLNGQDLMGQPISVDWCFVRGPPKGKRRGGRRRSRSPDRRRR 70BBD03CDDFEECFE rs6_human 40S ribosomal protein S6 RPS6 Phosphoprotein NP33 Small ribosomal subunit protein eS6 OK/SW-cl.2 protein protein human Homo sapiens Human MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK DIP protein P62753 original sequence version: 72 DIP protein P62753 original sequence version: 68 DIP protein P62753 original sequence version: 123 A61E435884E636AE ren3b_human Regulator of nonsense transcripts 3B RENT3B Nonsense mRNA reducing factor 3B Up-frameshift suppressor 3 homolog B UPF3X UPF3B Up-frameshift suppressor 3 homolog on chromosome X protein protein human Homo sapiens Human MKEEKEHRPKEKRVTLLTPAGATGSGGGTSGDSSKGEDKQDRNKEKKEALSKVVIRRLPPTLTKEQLQEHLQPMPEHDYFEFFSNDTSLYPHMYARAYINFKNQEDIILFRDRFDGYVFLDNKGQEYPAIVEFAPFQKAAKKKTKKRDTKVGTIDDDPEYRKFLESYATDNEKMTSTPETLLEEIEAKNRELIAKKTTPLLSFLKNKQRMREEKREERRRREIERKRQREEERRKWKEEEKRKRKDIEKLKKIDRIPERDKLKDEPKIKVHRFLLQAVNQKNLLKKPEKGDEKELDKREKAKKLDKENLSDERASGQSCTLPKRSDSELKDEKPKRPEDESGRDYREREREYERDQERILRERERLKRQEEERRRQKERYEKEKTFKRKEEEMKKEKDTLRDKGKKAESTESIGSSEKTEKKEEVVKRDRIRNKDRPAMQLYQPGARSRNRLCPPDDSTKSGDSAAERKQESGISHRKEGGEE DIP protein Q9BZI7 original sequence version: 74 DIP protein Q9BZI7 original sequence version: 75 F5A8A395783D1A69 rl23_human 60S ribosomal protein L23 RPL23 60S ribosomal protein L17 Large ribosomal subunit protein uL14 protein protein human Homo sapiens Human MSKRGRGGSSGAKFRISLGLPVGAVINCADNTGAKNLYIISVKGIKGRLNRLPAAGVGDMVMATVKKGKPELRKKVHPAVVIRQRKSYRRKDGVFLYFEDNAGVIVNNKGEMKGSAITGPVAKECADLWPRIASNAGSIA DIP protein P62829 original sequence version: 116 807E14139B2EB0B5 mgn_human Protein mago nashi homolog MAGOH MAGOHA protein protein human Homo sapiens Human MESDFYLRYYVGHKGKFGHEFLEFEFRPDGKLRYANNSNYKNDVMIRKEAYVHKSVMEELKRIIDDSEITKEDDALWPPPDRVGRQELEIVIGDEHISFTTSKIGSLIDVNQSKDPEGLRVFYYLVQDLKCLVFSLIGLHFKIKPI DIP protein P61326 original sequence version: 113 DIP protein P61326 original sequence version: 114 DIP protein P61326 original sequence version: 78 DIP protein P61326 original sequence version: 91 DIP protein P61326 original sequence version: 100 FFAD0B075E045875 if4a3_human Eukaryotic initiation factor 4A-III KIAA0111 EIF4A3 DDX48 Eukaryotic translation initiation factor 4A isoform 3 ATP-dependent RNA helicase eIF4A-3 ATP-dependent RNA helicase DDX48 DEAD box protein 48 Eukaryotic initiation factor 4A-like NUK-34 Nuclear matrix protein 265 protein protein human Homo sapiens Human MATTATMATSGSARKRLLKEEDMTKVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQVVLISATLPHEILEMTNKFMTDPIRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVPQVSLIINYDLPNNRELYIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI DIP protein P38919 original sequence version: 160 DIP protein P38919 original sequence version: 161 DIP protein P38919 original sequence version: 124 DIP protein P38919 original sequence version: 137 DIP protein P38919 original sequence version: 157 DIP protein P38919 original sequence version: 146 DIP protein P38919 original sequence version: 151 3A21888CA96CA5EA magoh-rbm8a-5 4 5 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P61326:p.Glu68Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E R Wed May 02 15:32:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu68arg' Tue Jun 30 20:15:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu68Arg' P61326:p.Glu117Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E R Wed May 02 15:24:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu117arg' Tue Jun 30 16:04:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu117Arg' P61326:p.Glu72_Asp73delinsArgLys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain ED RK Thu Jul 07 12:07:01 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was glu72arg_asp73lys Fri Jul 22 10:57:40 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'glu72arg,asp73lys' Wed May 02 18:28:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_asp72-73arg_lys' Tue Jun 30 16:14:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu72_Asp73delinsArgLys' 7 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Immunoprecipitations were done from RNase-A-treated lysates of HeLa cells that were transfected with FLAG-MAGOH, mutants of FLAG-MAGOH, or unfused FLAG as negative control together with V5-PYM and V5-Y14. Fig. 1B magoh-rbm8a-6 4 11 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Immunoprecipitations were done from RNase-A-treated lysates of HeLa cells that were transfected with FLAG-MAGOH, mutants of FLAG-MAGOH, or unfused FLAG as negative control together with V5-PYM and V5-Y14. Fig. 1B, 1C magoh-rbm8a-7 1 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal Strep tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) physical association physical association Purified recombinant PYM or PYM ΔN33 were used for His-pull downs with purified His-Y14ΔN50-MAGOH. Fig. 2D magoh-rbm8a-8 1 5 unspecified role unspecified role prey prey c-terminal Strep tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal Strep tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) physical association physical association Purified recombinant PYM or PYM ΔN33 were used for His-pull downs with purified His-Y14ΔN50-MAGOH. Fig. 2D magoh-rbm8a-9 4 11 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association PYM is associated only with MAGOH-Y14, Fig. 3F magoh-rbm8a-10 4 12 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association and correspondingly, BTZ, UPF3b, and eIF4A3 are found in MAGOH immunoprecipitations Fig. 3F eif4a3-rbm8a-1 4 9 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association In contrast, the EJC proteins BTZ and UPF3b are present in eIF4A3 immunoprecipitations, Fig. 3F wibg-rl23-1 3 8 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component colocalization colocalization The ribosomal association of PYM was confirmed by coimmunoprecipitation of two ribosomal proteins (RPS6 and RPL23) (Figure 4C). Fig. 4C wibg-rl23-2 2 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey association association Coprecipitated proteins were detected by immunoblotting using RPS6 and RPL23 antibodies, and FLAG-PYM was detected with a FLAG antibody. Fig. 4C wibg-rl23-3 3 5 unspecified role unspecified role neutral component neutral component region Venus tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role neutral component neutral component colocalization colocalization By contrast, the majority of PYM ΔC53 is found in the supernatant, confirming the importance of the C-terminal domain for ribosome binding (Diem et al., 2007 M.D. Diem, C.C. Chan, I. Younis and G. Dreyfuss, PYM binds the cytoplasmic exon-junction complex and ribosomes to enhance translation of spliced mRNAs, Nat. Struct. Mol. Biol. 14 (2007), pp. 1173�1179. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (8)Diem et al., 2007). Fig. 4D magoh-rbm8a-12 2 5 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Supp. Fig. 2 magoh-if4a3-1 2 11 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Supp. Fig. 2 magoh-rbm8a-13 2 11 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Supp. Fig. 2