IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Science (0036-8075) 2009 imex curation Park SY., Fung P., Nishimura N., Jensen DR., Fujii H., Zhao Y., Lumba S., Santiago J., Rodrigues A., Chow TF., Alfred SE., Bonetta D., Finkelstein R., Provart NJ., Desveaux D., Rodriguez PL., McCourt P., Zhu JK., Schroeder JI., Volkman BF., Cutler SR. The selection medium contained 15 mM 3-AT and 10 micro Molar pyrabactin. sean.cutler@ucr.edu 23-Dec-2009: Contacted by JYOTI. Arabidopsis etiolated seedling cDNA library. orchard Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Park SY., Fung P., Nishimura N., Jensen DR., Fujii H., Zhao Y., Lumba S., Santiago J., Rodrigues A., Chow TF., Alfred SE., Bonetta D., Finkelstein R., Provart NJ., Desveaux D., Rodriguez PL., McCourt P., Zhu JK., Schroeder JI., Volkman BF., Cutler SR. The selection medium contained 15 mM 3-AT and 10 micro Molar pyrabactin. sean.cutler@ucr.edu Accepted 2009-JUL-17 by ORCHARD. 23-Dec-2009: Contacted by JYOTI. Arabidopsis etiolated seedling cDNA library. Science (0036-8075) 2009 orchard Only protein-protein interactions imex curation Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Science (0036-8075) 2009 imex curation Park SY., Fung P., Nishimura N., Jensen DR., Fujii H., Zhao Y., Lumba S., Santiago J., Rodrigues A., Chow TF., Alfred SE., Bonetta D., Finkelstein R., Provart NJ., Desveaux D., Rodriguez PL., McCourt P., Zhu JK., Schroeder JI., Volkman BF., Cutler SR. The selection medium contained 15 mM 3-AT and 10 micro Molar pyrabactin. sean.cutler@ucr.edu 23-Dec-2009: Contacted by JYOTI. Arabidopsis etiolated seedling cDNA library. orchard Only protein-protein interactions nicbe Nicotiana benthamiana anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SY., Fung P., Nishimura N., Jensen DR., Fujii H., Zhao Y., Lumba S., Santiago J., Rodrigues A., Chow TF., Alfred SE., Bonetta D., Finkelstein R., Provart NJ., Desveaux D., Rodriguez PL., McCourt P., Zhu JK., Schroeder JI., Volkman BF., Cutler SR. The selection medium contained 15 mM 3-AT and 10 micro Molar pyrabactin. sean.cutler@ucr.edu Accepted 2009-JUL-17 by ORCHARD. 23-Dec-2009: Contacted by JYOTI. Arabidopsis etiolated seedling cDNA library. Science (0036-8075) 2009 orchard Only protein-protein interactions imex curation Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Science (0036-8075) 2009 imex curation Park SY., Fung P., Nishimura N., Jensen DR., Fujii H., Zhao Y., Lumba S., Santiago J., Rodrigues A., Chow TF., Alfred SE., Bonetta D., Finkelstein R., Provart NJ., Desveaux D., Rodriguez PL., McCourt P., Zhu JK., Schroeder JI., Volkman BF., Cutler SR. The selection medium contained 15 mM 3-AT and 10 micro Molar pyrabactin. sean.cutler@ucr.edu 23-Dec-2009: Contacted by JYOTI. Arabidopsis etiolated seedling cDNA library. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Park SY., Fung P., Nishimura N., Jensen DR., Fujii H., Zhao Y., Lumba S., Santiago J., Rodrigues A., Chow TF., Alfred SE., Bonetta D., Finkelstein R., Provart NJ., Desveaux D., Rodriguez PL., McCourt P., Zhu JK., Schroeder JI., Volkman BF., Cutler SR. The selection medium contained 15 mM 3-AT and 10 micro Molar pyrabactin. sean.cutler@ucr.edu Accepted 2009-JUL-17 by ORCHARD. 23-Dec-2009: Contacted by JYOTI. Arabidopsis etiolated seedling cDNA library. Science (0036-8075) 2009 orchard Only protein-protein interactions imex curation Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Science (0036-8075) 2009 imex curation Park SY., Fung P., Nishimura N., Jensen DR., Fujii H., Zhao Y., Lumba S., Santiago J., Rodrigues A., Chow TF., Alfred SE., Bonetta D., Finkelstein R., Provart NJ., Desveaux D., Rodriguez PL., McCourt P., Zhu JK., Schroeder JI., Volkman BF., Cutler SR. The selection medium contained 15 mM 3-AT and 10 micro Molar pyrabactin. sean.cutler@ucr.edu 23-Dec-2009: Contacted by JYOTI. Arabidopsis etiolated seedling cDNA library. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Park SY., Fung P., Nishimura N., Jensen DR., Fujii H., Zhao Y., Lumba S., Santiago J., Rodrigues A., Chow TF., Alfred SE., Bonetta D., Finkelstein R., Provart NJ., Desveaux D., Rodriguez PL., McCourt P., Zhu JK., Schroeder JI., Volkman BF., Cutler SR. The selection medium contained 15 mM 3-AT and 10 micro Molar pyrabactin. sean.cutler@ucr.edu Accepted 2009-JUL-17 by ORCHARD. 23-Dec-2009: Contacted by JYOTI. Arabidopsis etiolated seedling cDNA library. Science (0036-8075) 2009 orchard Only protein-protein interactions imex curation p2c78_arath Probable protein phosphatase 2C 78 SAG113 HAI1 Protein HIGHLY ABA-INDUCED 1 Protein SENESCENCE-ASSOCIATED GENE 113 MNC17.110 At5g59220 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAEICYENETMMIETTATVVKKATTTTRRRERSSSQAARRRRMEIRRFKFVSGEQEPVFVDGDLQRRRRRESTVAASTSTVFYETAKEVVVLCESLSSTVVALPDPEAYPKYGVASVCGRRREMEDAVAVHPFFSRHQTEYSSTGFHYCGVYDGHGCSHVAMKCRERLHELVREEFEADADWEKSMARSFTRMDMEVVALNADGAAKCRCELQRPDCDAVGSTAVVSVLTPEKIIVANCGDSRAVLCRNGKAIALSSDHKPDRPDELDRIQAAGGRVIYWDGPRVLGVLAMSRAIGDNYLKPYVISRPEVTVTDRANGDDFLILASDGLWDVVSNETACSVVRMCLRGKVNGQVSSSPEREMTGVGAGNVVVGGGDLPDKACEEASLLLTRLALARQSSDNVSVVVVDLRRDT DIP protein Q9FIF5 original sequence version: 44 DIP protein Q9FIF5 original sequence version: 45 DIP protein Q9FIF5 original sequence version: 92 649CF986D17A92D7 q8l837_arath Glucan endo-1,3-beta-D-glucosidase T9A21.190 T9A21_190 At4g18340 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTSRTFTRHSSLIHVFLLLSLVFSGNILQTVTSLGINYGQVGNNLPSPDKVINLLRSLRITKTRIYDTNPQILSAFANSNIEIIVTIENQVLPLLQDPQQATQWVDSHIKPYVPATRITGIMVGNELFTDDDSSLIGYMMPAIINIHKALVQLGLDRYIQVSSPSSLAVLGESYPPSAGSFKPEVSSVMQQLLDFLEATKSPFWINAYPYFAYKDNPQEIPVDYVLFNRNIGMTDPNTRLHYDNMMYAQVDAVAFAAAKLGYRNIEVRVAETGWPSKGDVGEIGASPVNAATYNRNLMMRQFAGEGTPARRNARLDVYIFALFNEDMKPGPTSEKNYGIFQPDGSLAYNLGFSTMSTTTANSESVTYSSSATKAKRSLEYWTILILAMVQVVMLRLF 7D88A9662B22C15B pyr1_arath Abscisic acid receptor PYR1 PYR1 ABIP6 At4g17870 RCAR11 Protein PYRABACTIN RESISTANCE 1 ABI1-binding protein 6 Regulatory components of ABA receptor 11 T6K21.50 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MPSELTPEERSELKNSIAEFHTYQLDPGSCSSLHAQRIHAPPELVWSIVRRFDKPQTYKHFIKSCSVEQNFEMRVGCTRDVIVISGLPANTSTERLDILDDERRVTGFSIIGGEHRLTNYKSVTTVHRFEKENRIWTVVLESYVVDMPEGNSEDDTRMFADTVVKLNLQKLATVAEAMARNSGDGSGSQVT DIP protein O49686 original sequence version: 50 DIP protein O49686 original sequence version: 63 DIP protein O49686 original sequence version: 54 DIP protein O49686 original sequence version: 92 AD35DA240E286B55 pyl9_arath Abscisic acid receptor PYL9 At1g01360 PYL9 RCAR1 PYR1-like protein 9 ABI1-binding protein 4 Regulatory components of ABA receptor 1 F6F3.16 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MMDGVEGGTAMYGGLETVQYVRTHHQHLCRENQCTSALVKHIKAPLHLVWSLVRRFDQPQKYKPFVSRCTVIGDPEIGSLREVNVKSGLPATTSTERLELLDDEEHILGIKIIGGDHRLKNYSSILTVHPEIIEGRAGTMVIESFVVDVPQGNTKDETCYFVEALIRCNLKSLADVSERLASQDITQ DIP protein Q84MC7 original sequence version: 69 070D485311163568 p2c56_arath Protein phosphatase 2C 56 Protein ABSCISIC ACID-INSENSITIVE 1 ABI1 At4g26080 F20B18.190 Protein phosphatase 2C ABI1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEVSPAIAGPFRPFSETQMDFTGIRLGKGYCNNQYSNQDSENGDLMVSLPETSSCSVSGSHGSESRKVLISRINSPNLNMKESAAADIVVVDISAGDEINGSDITSEKKMISRTESRSLFEFKSVPLYGFTSICGRRPEMEDAVSTIPRFLQSSSGSMLDGRFDPQSAAHFFGVYDGHGGSQVANYCRERMHLALAEEIAKEKPMLCDGDTWLEKWKKALFNSFLRVDSEIESVAPETVGSTSVVAVVFPSHIFVANCGDSRAVLCRGKTALPLSVDHKPDREDEAARIEAAGGKVIQWNGARVFGVLAMSRSIGDRYLKPSIIPDPEVTAVKRVKEDDCLILASDGVWDVMTDEEACEMARKRILLWHKKNAVAGDASLLADERRKEGKDPAAMSAAEYLSKLAIQRGSKDNISVVVVDLKPRRKLKSKPLN DIP protein P49597 original sequence version: 104 DIP protein P49597 original sequence version: 101 DIP protein P49597 original sequence version: 106 DIP protein P49597 original sequence version: 102 DIP protein P49597 original sequence version: 116 DIP protein P49597 original sequence version: 107 DIP protein P49597 original sequence version: 124 DIP protein P49597 original sequence version: 161 4A4C54F04195F572 p2c16_arath Protein phosphatase 2C 16 HAB1 P2C-HA Protein phosphatase 2C HAB1 AtP2C-HA Protein HYPERSENSITIVE TO ABA 1 F28P22.4 At1g72770 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEMTPAVAMTLSLAANTMCESSPVEITQLKNVTDAADLLSDSENQSFCNGGTECTMEDVSELEEVGEQDLLKTLSDTRSGSSNVFDEDDVLSVVEDNSAVISEGLLVVDAGSELSLSNTAMEIDNGRVLATAIIVGESSIEQVPTAEVLIAGVNQDTNTSEVVIRLPDENSNHLVKGRSVYELDCIPLWGTVSIQGNRSEMEDAFAVSPHFLKLPIKMLMGDHEGMSPSLTHLTGHFFGVYDGHGGHKVADYCRDRLHFALAEEIERIKDELCKRNTGEGRQVQWDKVFTSCFLTVDGEIEGKIGRAVVGSSDKVLEAVASETVGSTAVVALVCSSHIVVSNCGDSRAVLFRGKEAMPLSVDHKPDREDEYARIENAGGKVIQWQGARVFGVLAMSRSIGDRYLKPYVIPEPEVTFMPRSREDECLILASDGLWDVMNNQEVCEIARRRILMWHKKNGAPPLAERGKGIDPACQAAADYLSMLALQKGSKDNISIIVIDLKAQRKFKTRT DIP protein Q9CAJ0 original sequence version: 64 DIP protein Q9CAJ0-1 original sequence version: 62 DIP protein Q9CAJ0-1 original sequence version: 77 DIP protein Q9CAJ0 original sequence version: 62 DIP protein Q9CAJ0 original sequence version: 65 DIP protein Q9CAJ0-1 original sequence version: 66 DIP protein Q9CAJ0 original sequence version: 81 DIP protein Q9CAJ0-1 original sequence version: 86 DIP protein Q9CAJ0-1 original sequence version: 108 DIP protein Q9CAJ0 original sequence version: 112 DIP protein Q9CAJ0-1 original sequence version: 81 B001BAF0DD8333E1 p2c75_arath Probable protein phosphatase 2C 75 AHG1 Protein phosphatase 2C AHG1 Protein ABA-HYPERSENSITIVE GERMINATION 1 MIO24.11 At5g51760 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTEIYRTISTGRGDDVSPTKCRERRRRRIEMRRQAAVFGEPSSSRNRDRTDMEVYSSFDVPLRKQARRSEIGGLPADIGGFLAPPAASSCQKSEAPVWKGEETEDEPLYGIVSVMGRSRKMEDSVTVKPNLCKPEVNRQRPVHFFAVYDGHGGSQVSTLCSTTMHTFVKEELEQNLEEEEEGSENDVVERKWRGVMKRSFKRMDEMATSTCVCGTSVPLCNCDPREAAISGSTAVTAVLTHDHIIVANTGDSRAVLCRNGMAIPLSNDHKPDRPDERARIEAAGGRVLVVDGARVEGILATSRAIGDRYLKPMVAWEPEVTFMRRESGDECLVLASDGLWDVLSSQLACDIARFCLREETPSSLDLNRMAQEDDNDGEQNPSRSVLAATLLTRLALGRQSSDNISVVVIDLKNSSQ DIP protein Q9FLI3 original sequence version: 53 DIP protein Q9FLI3 original sequence version: 98 7272F86C929FF020 pyl5_arath Abscisic acid receptor PYL5 At5g05440 PYL5 ABIP3 RCAR8 PYR1-like protein 5 ABI1-binding protein 3 Regulatory components of ABA receptor 8 K18I23.25 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MRSPVQLQHGSDATNGFHTLQPHDQTDGPIKRVCLTRGMHVPEHVAMHHTHDVGPDQCCSSVVQMIHAPPESVWALVRRFDNPKVYKNFIRQCRIVQGDGLHVGDLREVMVVSGLPAVSSTERLEILDEERHVISFSVVGGDHRLKNYRSVTTLHASDDEGTVVVESYIVDVPPGNTEEETLSFVDTIVRCNLQSLARSTNRQ DIP protein Q9FLB1 original sequence version: 46 DIP protein Q9FLB1 original sequence version: 51 5A446F5E40D5AF11 pyl1_arath Abscisic acid receptor PYL1 At5g46790 PYL1 RCAR12 PYR1-like protein 1 ABI1-binding protein 6 Regulatory components of ABA receptor 9 MZA15.21 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MANSESSSSPVNEEENSQRISTLHHQTMPSDLTQDEFTQLSQSIAEFHTYQLGNGRCSSLLAQRIHAPPETVWSVVRRFDRPQIYKHFIKSCNVSEDFEMRVGCTRDVNVISGLPANTSRERLDLLDDDRRVTGFSITGGEHRLRNYKSVTTVHRFEKEEEEERIWTVVLESYVVDVPEGNSEEDTRLFADTVIRLNLQKLASITEAMNRNNNNNNSSQVR DIP protein Q8VZS8 original sequence version: 33 DIP protein Q8VZS8 original sequence version: 38 DIP protein Q8VZS8 original sequence version: 34 DIP protein Q8VZS8 original sequence version: 39 B64B1A52C43072C2 p2c37_arath Protein phosphatase 2C 37 F24K9.8 At3g11410 PP2CA AHG3 Protein ABA-HYPERSENSITIVE GERMINATION 3 Protein phosphatase 2C A protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAGICCGVVGETEPAAPVDSTSRASLRRRLDLLPSIKIVADSAVAPPLENCRKRQKRETVVLSTLPGNLDLDSNVRSENKKARSAVTNSNSVTEAESFFSDVPKIGTTSVCGRRRDMEDAVSIHPSFLQRNSENHHFYGVFDGHGCSHVAEKCRERLHDIVKKEVEVMASDEWTETMVKSFQKMDKEVSQRECNLVVNGATRSMKNSCRCELQSPQCDAVGSTAVVSVVTPEKIIVSNCGDSRAVLCRNGVAIPLSVDHKPDRPDELIRIQQAGGRVIYWDGARVLGVLAMSRAIGDNYLKPYVIPDPEVTVTDRTDEDECLILASDGLWDVVPNETACGVARMCLRGAGAGDDSDAAHNACSDAALLLTKLALARQSSDNVSVVVVDLRKRRNNQASS DIP protein P49598 original sequence version: 79 DIP protein P49598 original sequence version: 80 DIP protein P49598 original sequence version: 95 DIP protein P49598 original sequence version: 130 83B82E32FEC71D4D pyl2_arath Abscisic acid receptor PYL2 PYL2 RCAR14 PYR1-like protein 2 Regulatory components of ABA receptor 14 T19L18.15 At2g26040 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSSSPAVKGLTDEEQKTLEPVIKTYHQFEPDPTTCTSLITQRIHAPASVVWPLIRRFDNPERYKHFVKRCRLISGDGDVGSVREVTVISGLPASTSTERLEFVDDDHRVLSFRVVGGEHRLKNYKSVTSVNEFLNQDSGKVYTVVLESYTVDIPEGNTEEDTKMFVDTVVKLNLQKLGVAATSAPMHDDE DIP protein O80992 original sequence version: 50 DIP protein O80992 original sequence version: 47 DIP protein O80992 original sequence version: 51 DIP protein O80992 original sequence version: 69 DIP protein O80992 original sequence version: 65 CBB16E64BE2B8164 srk2d_arath Serine/threonine-protein kinase SRK2D SRK2D OSKL3 SNRK2.2 SPK2 Protein ATHPROKIN A OST1-kinase-like 3 SNF1-related kinase 2.2 T20E23.100 At3g50500 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDPATNSPIMPIDLPIMHDSDRYDFVKDIGSGNFGVARLMTDRVTKELVAVKYIERGEKIDENVQREIINHRSLRHPNIVRFKEVILTPSHLAIVMEYAAGGELYERICNAGRFSEDEARFFFQQLISGVSYCHAMQICHRDLKLENTLLDGSPAPRLKICDFGYSKSSVLHSQPKSTVGTPAYIAPEILLRQEYDGKLADVWSCGVTLYVMLVGAYPFEDPQEPRDYRKTIQRILSVTYSIPEDLHLSPECRHLISRIFVADPATRITIPEITSDKWFLKNLPGDLMDENRMGSQFQEPEQPMQSLDTIMQIISEATIPTVRNRCLDDFMADNLDLDDDMDDFDSESEIDVDSSGEIVYAL DIP protein Q39192 original sequence version: 70 DIP protein Q39192 original sequence version: 69 DIP protein Q39192 original sequence version: 72 043E44E476E17119 pyl6_arath Abscisic acid receptor PYL6 T3G21 At2g40330 PYL6 ABIP5 RCAR9 PYR1-like protein 6 ABI1-binding protein 5 Regulatory components of ABA receptor 9 T7M7.15 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MPTSIQFQRSSTAAEAANATVRNYPHHHQKQVQKVSLTRGMADVPEHVELSHTHVVGPSQCFSVVVQDVEAPVSTVWSILSRFEHPQAYKHFVKSCHVVIGDGREVGSVREVRVVSGLPAAFSLERLEIMDDDRHVISFSVVGGDHRLMNYKSVTTVHESEEDSDGKKRTRVVESYVVDVPAGNDKEETCSFADTIVRCNLQSLAKLAENTSKFS DIP protein Q8S8E3 original sequence version: 35 DIP protein Q8S8E3 original sequence version: 40 8B9D3F7BD9E2860D pyl4_arath Abscisic acid receptor PYL4 At2g38310 PYL4 ABIP2 RCAR10 PYR1-like protein 4 ABI1-binding protein 2 Regulatory components of ABA receptor 10 T19C21.20 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLAVHRPSSAVSDGDSVQIPMMIASFQKRFPSLSRDSTAARFHTHEVGPNQCCSAVIQEISAPISTVWSVVRRFDNPQAYKHFLKSCSVIGGDGDNVGSLRQVHVVSGLPAASSTERLDILDDERHVISFSVVGGDHRLSNYRSVTTLHPSPISGTVVVESYVVDVPPGNTKEETCDFVDVIVRCNLQSLAKIAENTAAESKKKMSL DIP protein O80920 original sequence version: 50 DIP protein O80920 original sequence version: 54 DIP protein O80920 original sequence version: 68 118160B35D7066BB pyl7_arath Abscisic acid receptor PYL7 PYL7 ABIP7 RCAR2 PYR1-like protein 7 ABI1-binding protein 7 Regulatory components of ABA receptor 2 F3I3 At4g01026 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEMIGGDDTDTEMYGALVTAQSLRLRHLHHCRENQCTSVLVKYIQAPVHLVWSLVRRFDQPQKYKPFISRCTVNGDPEIGCLREVNVKSGLPATTSTERLEQLDDEEHILGINIIGGDHRLKNYSSILTVHPEMIDGRSGTMVMESFVVDVPQGNTKDDTCYFVESLIKCNLKSLACVSERLAAQDITNSIATFCNASNGYREKNHTETNL 6381793C94A43C32 pyl10_arath Abscisic acid receptor PYL10 PYL10 RCAR4 PYR1-like protein 10 ABI1-binding protein 8 Regulatory components of ABA receptor 4 T13J8.30 At4g27920 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MNGDETKKVESEYIKKHHRHELVESQCSSTLVKHIKAPLHLVWSIVRRFDEPQKYKPFISRCVVQGKKLEVGSVREVDLKSGLPATKSTEVLEILDDNEHILGIRIVGGDHRLKNYSSTISLHSETIDGKTGTLAIESFVVDVPEGNTKEETCFFVEALIQCNLNSLADVTERLQAESMEKKI 896A016CA9D2752F pyl3_arath Abscisic acid receptor PYL3 PYL3 RCAR13 PYR1-like protein 3 Regulatory components of ABA receptor 13 F3N23.20 At1g73000 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MNLAPIHDPSSSSTTTTSSSTPYGLTKDEFSTLDSIIRTHHTFPRSPNTCTSLIAHRVDAPAHAIWRFVRDFANPNKYKHFIKSCTIRVNGNGIKEIKVGTIREVSVVSGLPASTSVEILEVLDEEKRILSFRVLGGEHRLNNYRSVTSVNEFVVLEKDKKKRVYSVVLESYIVDIPQGNTEEDTRMFVDTVVKSNLQNLAVISTASPT DIP protein Q9SSM7 original sequence version: 37 DIP protein Q9SSM7 original sequence version: 43 DIP protein Q9SSM7 original sequence version: 59 4A25A6E47D469890 pyl11_arath Abscisic acid receptor PYL11 At5g45860 PYL11 RCAR5 PYR1-like protein 11 Regulatory components of ABA receptor 5 K15I22.6 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress METSQKYHTCGSTLVQTIDAPLSLVWSILRRFDNPQAYKQFVKTCNLSSGDGGEGSVREVTVVSGLPAEFSRERLDELDDESHVMMISIIGGDHRLVNYRSKTMAFVAADTEEKTVVVESYVVDVPEGNSEEETTSFADTIVGFNLKSLAKLSERVAHLKL C2DC792735D5F200 pyl12_arath Abscisic acid receptor PYL12 PYL12 RCAR6 At5g45870 PYR1-like protein 12 Regulatory components of ABA receptor 6 K15I22.7 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKTSQEQHVCGSTVVQTINAPLPLVWSILRRFDNPKTFKHFVKTCKLRSGDGGEGSVREVTVVSDLPASFSLERLDELDDESHVMVISIIGGDHRLVNYQSKTTVFVAAEEEKTVVVESYVVDVPEGNTEEETTLFADTIVGCNLRSLAKLSEKMMELT BEFDC3CDF077ABD0 p2c77_arath Protein phosphatase 2C 77 Protein ABSCISIC ACID-INSENSITIVE 2 ABI2 At5g57050 MHM17.19 Protein phosphatase 2C ABI2 PP2C77 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDEVSPAVAVPFRPFTDPHAGLRGYCNGESRVTLPESSCSGDGAMKDSSFEINTRQDSLTSSSSAMAGVDISAGDEINGSDEFDPRSMNQSEKKVLSRTESRSLFEFKCVPLYGVTSICGRRPEMEDSVSTIPRFLQVSSSSLLDGRVTNGFNPHLSAHFFGVYDGHGGSQVANYCRERMHLALTEEIVKEKPEFCDGDTWQEKWKKALFNSFMRVDSEIETVAHAPETVGSTSVVAVVFPTHIFVANCGDSRAVLCRGKTPLALSVDHKPDRDDEAARIEAAGGKVIRWNGARVFGVLAMSRSIGDRYLKPSVIPDPEVTSVRRVKEDDCLILASDGLWDVMTNEEVCDLARKRILLWHKKNAMAGEALLPAEKRGEGKDPAAMSAAEYLSKMALQKGSKDNISVVVVDLKGIRKFKSKSLN 67CAAC76DA531A71 pyr1-hab1 4 10 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role bait bait physical association physical association This revealed that the PP2C HAB1 (28, 29) interacts with PYR1 in response to pyrabactin and (+)-ABA, but not the inactive analog apyrabactin or several other plant hormones pyr1-hab1-1 3 7 unspecified role unspecified role bait bait O49686:p.Ser152Leu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain S L Wed May 02 15:47:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser152leu' Tue Jun 30 19:12:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser152Leu' O49686:p.Pro88Ser mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain P S Wed May 02 19:21:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro88ser' Tue Jun 30 18:01:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro88Ser' 10 unspecified role unspecified role prey prey physical association physical association This revealed that the PP2C HAB1 (28, 29) interacts with PYR1 in response to pyrabactin and (+)-ABA, but not the inactive analog apyrabactin or several other plant hormones Pyrabactin. ABA (Abscisic acid). Figure 3A Supplemenatry Figure 4A Dimethyl sulfoxide (DMSO) Apyrabactin Epibrassinolide Gillberellic Acid (GA) Methyl jasmonate MejA Kinetin 2,4-Dichlorophenoxyacetic acid (2,4-D) pyr1-abi1 3 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Pyrabactin. ABA (Abscisic acid). Figure 3A Supplementary Table 2 pyr1-abi2 3 7 unspecified role unspecified role bait bait 24 unspecified role unspecified role prey prey O04719:p.Gly168Asp mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain G D Wed May 02 17:05:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly168asp' Tue Jun 30 16:02:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly168Asp' physical association physical association Pyrabactin. ABA (Abscisic acid). Figure 3A pyr1-hab1-2 2 10 unspecified role unspecified role prey prey region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait O49686:p.Pro88Ser mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain P S Wed May 02 15:43:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro88ser' Tue Jun 30 19:31:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro88Ser' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association ABA (Abscisic acid). Figure 3B pyr1-abi1-1 2 7 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey P49597:p.Gly180Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G D Wed May 02 15:05:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly180asp' Tue Jun 30 17:19:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly180Asp' region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association ABA (Abscisic acid). Figure 3B pyr1-abi2-1 2 7 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 24 unspecified role unspecified role prey prey region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association ABA (Abscisic acid). Figure 3B abi1-pyl4 3 9 unspecified role unspecified role bait bait 18 unspecified role unspecified role prey prey physical association physical association ABA (Abscisic acid). Figure 3C Supplementary Table 2 abi1-pyr1 3 9 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association ABA (Abscisic acid). Figure 3C pyl1-hab1-3 3 10 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait physical association physical association Pyrabactin. ABA (Abscisic acid). Supplemenatry Figure 4A Dimethyl sulfoxide (DMSO) Apyrabactin Epibrassinolide Gillberellic Acid (GA) Methyl jasmonate MejA Kinetin 2,4-Dichlorophenoxyacetic acid (2,4-D) pyl2-hab1 3 15 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Supplemenatry Figure 4A Dimethyl sulfoxide (DMSO) Pyrabactin Apyrabactin Epibrassinolide Gillberellic Acid (GA) Methyl jasmonate MejA Kinetin 2,4-Dichlorophenoxyacetic acid (2,4-D) pyl3-hab1 3 21 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Supplemenatry Figure 4A Dimethyl sulfoxide (DMSO) Pyrabactin reduces interaction as seen by reduced reporter gene activity. Apyrabactin Epibrassinolide Gillberellic Acid (GA) Methyl jasmonate MejA Kinetin 2,4-Dichlorophenoxyacetic acid (2,4-D) pyl4-hab1 3 10 unspecified role unspecified role prey prey 18 unspecified role unspecified role bait bait physical association physical association Supplemenatry Figure 4A Dimethyl sulfoxide (DMSO) Apyrabactin Epibrassinolide Gillberellic Acid (GA) Methyl jasmonate MejA Kinetin 2,4-Dichlorophenoxyacetic acid (2,4-D) Pyrabactin pyl5-hab1 3 12 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Supplemenatry Figure 4A pyl6-hab1-1 3 10 unspecified role unspecified role prey prey 17 unspecified role unspecified role bait bait physical association physical association Supplemenatry Figure 4A pyl7-hab1 3 19 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Supplemenatry Figure 4A pyl10-hab1 3 20 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Supplemenatry Figure 4A pyl9-hab1-1 3 8 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Supplemenatry Figure 4A pyl11-hab1 3 22 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Supplemenatry Figure 4A pyl12-hab1 3 23 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Supplemenatry Figure 4A ahg3-pyr1 3 7 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey physical association physical association ABA (Abscisic acid). Supplementary Figure 4B Supplementary Table 2 ahg3-pyl1 3 13 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey physical association physical association ABA (Abscisic acid). Supplementary Figure 4B ahg3-pyl2 3 15 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey physical association physical association ABA (Abscisic acid). Supplementary Figure 4B ahg3-pyl3 3 21 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey physical association physical association Supplementary Figure 4B Dimethyl sulfoxide (DMSO) Pyrabactin Apyrabactin ahg3-pyl4 3 18 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey physical association physical association Supplementary Figure 4B Supplementary Table 2 Abscisic acid (ABA) ahg3-pyl12 3 14 unspecified role unspecified role prey prey 23 unspecified role unspecified role bait bait physical association physical association Supplementary Figure 4B Abscisic acid (ABA) ahg3-snrk2_2 3 16 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Supplementary Figure 4C abi1-snrk2_2 3 16 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association Supplementary Figure 4C ahg1-snrk2_2 3 16 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association Supplementary Figure 4C pyr1-at5g59220 3 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association ABA (Abscisic acid). Supplementary Table 2 pyl4-at5g59220 3 5 unspecified role unspecified role prey prey 18 unspecified role unspecified role bait bait physical association physical association ABA (Abscisic acid). Supplementary Table 2 pyr1-at4g18340 3 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Supplementary Table 2 pyl4-at4g18340 3 6 unspecified role unspecified role prey prey 18 unspecified role unspecified role bait bait physical association physical association Supplementary Table 2 pyr1-hab1-3 1 7 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 3D ABA (Abscissic acid) pyr1-abi1-2 1 9 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association ABA (Abscisic acid). Figure 3B pyr1-abi2-2 1 24 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association ABA (Abscisic acid). Figure 3D