IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Micropreparative fractionation of the complexome by blue native continuous elution electrophoresis. Micropreparative fractionation of the complexome by blue native continuous elution electrophoresis. Proteomics (1615-9853) 2009 imex curation Huang KY., Filarsky M., Padula MP., Raftery MJ., Herbert BR., Wilkins MR. m.wilkins@unsw.edu.au 10-Jul-2009: Contacted by JYOTI. jyoti Only protein-protein interactions 9 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast bn-page blue native page bn-page fingerprinting peptide massfingerprinting fingerprinting Huang KY., Filarsky M., Padula MP., Raftery MJ., Herbert BR., Wilkins MR. m.wilkins@unsw.edu.au Accepted 2009-JUN-27 by JYOTI. Proteomics (1615-9853) 2009 jyoti Only protein-protein interactions 9 imex curation cysd_yeast Homocysteine/cysteine synthase MET17 MET25 MET15 O-acetylserine/O-acetylhomoserine sulfhydrylase L8003.1 YLR303W protein protein yeast Saccharomyces cerevisiae Baker's yeast MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQLFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNIVSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNVPDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGIIVDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFASFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNGFGGVLSFGVKDLPNADKETDPFKLSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQLNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP F84224625C921B35 adh1_yeast Alcohol dehydrogenase 1 ADH1 ADC1 YOL086C O0947 Alcohol dehydrogenase I YADH-1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSIPETQKGVIFYESHGKLEYKDIPVPKPKANELLINVKYSGVCHTDLHAWHGDWPLPVKLPLVGGHEGAGVVVGMGENVKGWKIGDYAGIKWLNGSCMACEYCELGNESNCPHADLSGYTHDGSFQQYATADAVQAAHIPQGTDLAQVAPILCAGITVYKALKSANLMAGHWVAISGAAGGLGSLAVQYAKAMGYRVLGIDGGEGKEELFRSIGGEVFIDFTKEKDIVGAVLKATDGGAHGVINVSVSEAAIEASTRYVRANGTTVLVGMPAGAKCCSDVFNQVVKSISIVGSYVGNRADTREALDFFARGLVKSPIKVVGLSTLPEIYEKMEKGQIVGRYVVDTSK F14AE24B5D8D12A5 F14AE24B5D8D12A5 ampl_yeast Vacuolar aminopeptidase 1 APE1 API Aminopeptidase yscI LAP4 Leucine aminopeptidase IV Lysosomal aminopeptidase III Polypeptidase Vacuolar aminopeptidase I YSC1 YKL455 YKL103C protein protein yeast Saccharomyces cerevisiae Baker's yeast MEEQREILEQLKKTLQMLTVEPSKNNQIANEEKEKKENENSWCILEHNYEDIAQEFIDFIYKNPTTYHVVSFFAELLDKHNFKYLSEKSNWQDSIGEDGGKFYTIRNGTNLSAFILGKNWRAEKGVGVIGSHVDALTVKLKPVSFKDTAEGYGRIAVAPYGGTLNELWLDRDLGIGGRLLYKKKGTNEIKSALVDSTPLPVCRIPSLAPHFGKPAEGPFDKEDQTIPVIGFPTPDEEGNEPPTDDEKKSPLFGKHCIHLLRYVAKLAGVEVSELIQMDLDLFDVQKGTIGGIGKHFLFAPRLDDRLCSFAAMIALICYAKDVNTEESDLFSTVTLYDNEEIGSLTRQGAKGGLLESVVERSSSAFTKKPVDLHTVWANSIILSADVNHLYNPNFPEVYLKNHFPVPNVGITLSLDPNGHMATDVVGTALVEELARRNGDKVQYFQIKNNSRSGGTIGPSLASQTGARTIDLGIAQLSMHSIRAATGSKDVGLGVKFFNGFFKHWRSVYDEFGEL 702A8C88A2124C24 702A8C88A2124C24 tsa1_yeast Peroxiredoxin TSA1 TSA1 TPX1 TSA YML028W Cytoplasmic thiol peroxidase 1 Thiol-specific antioxidant protein 1 ZRG14 Protector protein Thioredoxin peroxidase type Ia Thioredoxin-dependent peroxide reductase Thioredoxin-dependent peroxiredoxin TSA1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVAQVQKQAPTFKKTAVVDGVFDEVSLDKYKGKYVVLAFIPLAFTFVCPTEIIAFSEAAKKFEEQGAQVLFASTDSEYSLLAWTNIPRKEGGLGPINIPLLADTNHSLSRDYGVLIEEEGVALRGLFIIDPKGVIRHITINDLPVGRNVDEALRLVEAFQWTDKNGTVLPCNWTPGAATIKPTVEDSKEYFEAANK 9ACF40E410F2C04A 9ACF40E410F2C04A idh2_yeast Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial Isocitric dehydrogenase NAD(+)-specific ICDH IDH2 YOR136W YOR3326W O3326 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLRNTFFRNTSRRFLATVKQPSIGRYTGKPNPSTGKYTVSFIEGDGIGPEISKSVKKIFSAANVPIEWESCDVSPIFVNGLTTIPDPAVQSITKNLVALKGPLATPIGKGHRSLNLTLRKTFGLFANVRPAKSIEGFKTTYENVDLVLIRENTEGEYSGIEHIVCPGVVQSIKLITRDASERVIRYAFEYARAIGRPRVIVVHKSTIQRLADGLFVNVAKELSKEYPDLTLETELIDNSVLKVVTNPSAYTDAVSVCPNLYGDILSDLNSGLSAGSLGLTPSANIGHKISIFEAVHGSAPDIAGQDKANPTALLLSSVMMLNHMGLTNHADQIQNAVLSTIASGPENRTGDLAGTATTSSFTEAVIKRL 3A48C999776CE373 3A48C999776CE373 aldh6_yeast Magnesium-activated aldehyde dehydrogenase, cytosolic ALD6 ALDH1 YPL061W LPE9 Mg(2+)-activated acetaldehyde dehydrogenase protein protein yeast Saccharomyces cerevisiae Baker's yeast MTKLHFDTAEPVKITLPNGLTYEQPTGLFINNKFMKAQDGKTYPVEDPSTENTVCEVSSATTEDVEYAIECADRAFHDTEWATQDPRERGRLLSKLADELESQIDLVSSIEALDNGKTLALARGDVTIAINCLRDAAAYADKVNGRTINTGDGYMNFTTLEPIGVCGQIIPWNFPIMMLAWKIAPALAMGNVCILKPAAVTPLNALYFASLCKKVGIPAGVVNIVPGPGRTVGAALTNDPRIRKLAFTGSTEVGKSVAVDSSESNLKKITLELGGKSAHLVFDDANIKKTLPNLVNGIFKNAGQICSSGSRIYVQEGIYDELLAAFKAYLETEIKVGNPFDKANFQGAITNRQQFDTIMNYIDIGKKEGAKILTGGEKVGDKGYFIRPTVFYDVNEDMRIVKEEIFGPVVTVAKFKTLEEGVEMANSSEFGLGSGIETESLSTGLKVAKMLKAGTVWINTYNDFDSRVPFGGVKQSGYGREMGEEVYHAYTEVKAVRIKL 542AA956EFA0E676 542AA956EFA0E676 c1tc_yeast C-1-tetrahydrofolate synthase, cytoplasmic ADE3 G7733 YGR204W protein protein yeast Saccharomyces cerevisiae Baker's yeast MAGQVLDGKACAQQFRSNIANEIKSIQGHVPGFAPNLAIIQVGNRPDSATYVRMKRKAAEEAGIVANFIHLDESATEFEVLRYVDQLNEDPHTHGIIVQLPLPAHLDEDRITSRVLAEKDVDGFGPTNIGELNKKNGHPFFLPCTPKGIIELLHKANVTIEGSRSVVIGRSDIVGSPVAELLKSLNSTVTITHSKTRDIASYLHDADIVVVAIGQPEFVKGEWFKPRDGTSSDKKTVVIDVGTNYVADPSKKSGFKCVGDVEFNEAIKYVHLITPVPGGVGPMTVAMLMQNTLIAAKRQMEESSKPLQIPPLPLKLLTPVPSDIDISRAQQPKLINQLAQELGIYSHELELYGHYKAKISPKVIERLQTRQNGKYILVSGITPTPLGEGKSTTTMGLVQALTAHLGKPAIANVRQPSLGPTLGVKGGAAGGGYSQVIPMDEFNLHLTGDIHAIGAANNLLAAAIDTRMFHETTQKNDATFYNRLVPRKNGKRKFTPSMQRRLNRLGIQKTNPDDLTPEEINKFARLNIDPDTITIKRVVDINDRMLRQITIGQAPTEKNHTRVTGFDITVASELMAILALSKDLRDMKERIGRVVVAADVNRSPVTVEDVGCTGALTALLRDAIKPNLMQTLEGTPVLVHAGPFANISIGASSVIADRVALKLVGTEPEAKTEAGYVVTEAGFDFTMGGERFFNIKCRSSGLTPNAVVLVATVRALKSHGGAPDVKPGQPLPSAYTEENIEFVEKGAANMCKQIANIKQFGVPVVVAINKFETDTEGEIAAIRKAALEAGAFEAVTSNHWAEGGKGAIDLAKAVIEASNQPVDFHFLYDVNSSVEDKLTTIVQKMYGGAAIDILPEAQRKIDMYKEQGFGNLPICIAKTQYSLSHDATLKGVPTGFTFPIRDVRLSNGAGYLYALAAEIQTIPGLATYAGYMAVEVDDDGEIDGLF BC2897EC380CBA6F BC2897EC380CBA6F thil_yeast Acetyl-CoA acetyltransferase ERG10 YPL028W Acetoacetyl-CoA thiolase LPB3 Ergosterol biosynthesis protein 10 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQNVYIVSTARTPIGSFQGSLSSKTAVELGAVALKGALAKVPELDASKDFDEIIFGNVLSANLGQAPARQVALAAGLSNHIVASTVNKVCASAMKAIILGAQSIKCGNADVVVAGGCESMTNAPYYMPAARAGAKFGQTVLVDGVERDGLNDAYDGLAMGVHAEKCARDWDITREQQDNFAIESYQKSQKSQKEGKFDNEIVPVTIKGFRGKPDTQVTKDEEPARLHVEKLRSARTVFQKENGTVTAANASPINDGAAAVILVSEKVLKEKNLKPLAIIKGWGEAAHQPADFTWAPSLAVPKALKHAGIEDINSVDYFEFNEAFSVVGLVNTKILKLDPSKVNVYGGAVALGHPLGCSGARVVVTLLSILQQEGGKIGVAAICNGGGGASSIVIEKI E835A8E040629A5C E835A8E040629A5C idh1_yeast Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial IDH1 YNL037C N2690 Isocitric dehydrogenase NAD(+)-specific ICDH protein protein yeast Saccharomyces cerevisiae Baker's yeast MLNRTIAKRTLATAAQAERTLPKKYGGRFTVTLIPGDGVGKEITDSVRTIFEAENIPIDWETINIKQTDHKEGVYEAVESLKRNKIGLKGLWHTPADQTGHGSLNVALRKQLDIYANVALFKSLKGVKTRIPDIDLIVIRENTEGEFSGLEHESVPGVVESLKVMTRPKTERIARFAFDFAKKYNRKSVTAVHKANIMKLGDGLFRNIITEIGQKEYPDIDVSSIIVDNASMQAVAKPHQFDVLVTPSMYGTILGNIGAALIGGPGLVAGANFGRDYAVFEPGSRHVGLDIKGQNVANPTAMILSSTLMLNHLGLNEYATRISKAVHETIAEGKHTTRDIGGSSSTTDFTNEIINKLSTM 0932E7B3CD685240 0932E7B3CD685240 hsp60_yeast Heat shock protein 60, mitochondrial HSP60 MIF4 YLR259C L8479.10 Stimulator factor I 66 kDa component P66 CPN60 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLRSSVVRSRATLRPLLRRAYSSHKELKFGVEGRASLLKGVETLAEAVAATLGPKGRNVLIEQPFGPPKITKDGVTVAKSIVLKDKFENMGAKLLQEVASKTNEAAGDGTTSATVLGRAIFTESVKNVAAGCNPMDLRRGSQVAVEKVIEFLSANKKEITTSEEIAQVATISANGDSHVGKLLASAMEKVGKEGVITIREGRTLEDELEVTEGMRFDRGFISPYFITDPKSSKVEFEKPLLLLSEKKISSIQDILPALEISNQSRRPLLIIAEDVDGEALAACILNKLRGQVKVCAVKAPGFGDNRKNTIGDIAVLTGGTVFTEELDLKPEQCTIENLGSCDSITVTKEDTVILNGSGPKEAIQERIEQIKGSIDITTTNSYEKEKLQERLAKLSGGVAVIRVGGASEVEVGEKKDRYDDALNATRAAVEEGILPGGGTALVKASRVLDEVVVDNFDQKLGVDIIRKAITRPAKQIIENAGEEGSVIIGKLIDEYGDDFAKGYDASKSEYTDMLATGIIDPFKVVRSGLVDASGVASLLATTEVAIVDAPEPPAAAGAGGMPGGMPGMPGMM 279DC3AF5A2B0A3E 279DC3AF5A2B0A3E dhe4_yeast NADP-specific glutamate dehydrogenase 1 GDH1 URE1 YOR375C NADP-dependent glutamate dehydrogenase 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEPEFQQAYEEVVSSLEDSTLFEQHPEYRKVLPIVSVPERIIQFRVTWENDKGEQEVAQGYRVQYNSAKGPYKGGLRFHPSVNLSILKFLGFEQIFKNSLTGLDMGGGKGGLCVDLKGRSNNEIRRICYAFMRELSRHIGQDTDVPAGDIGVGGREIGYLFGAYRSYKNSWEGVLTGKGLNWGGSLIRPEATGYGLVYYTQAMIDYATNGKESFEGKRVTISGSGNVAQYAALKVIELGGTVVSLSDSKGCIISETGITSEQVADISSAKVNFKSLEQIVNEYSTFSENKVQYIAGARPWTHVQKVDIALPCATQNEVSGEEAKALVAQGVKFIAEGSNMGSTPEAIAVFETARSTATGPSEAVWYGPPKAANLGGVAVSGLEMAQNSQRITWTSERVDQELKRIMINCFNECIDYAKKYTKDGKVLPSLVKGANIASFIKVSDAMFDQGDVF 707F921B6FE5B5B1 707F921B6FE5B5B1 g3p3_yeast Glyceraldehyde-3-phosphate dehydrogenase 3 TDH3 GPD3 YGR192C G7576 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVRVAINGFGRIGRLVMRIALSRPNVEVVALNDPFITNDYAAYMFKYDSTHGRYAGEVSHDDKHIIVDGKKIATYQERDPANLPWGSSNVDIAIDSTGVFKELDTAQKHIDAGAKKVVITAPSSTAPMFVMGVNEEKYTSDLKIVSNASCTTNCLAPLAKVINDAFGIEEGLMTTVHSLTATQKTVDGPSHKDWRGGRTASGNIIPSSTGAAKAVGKVLPELQGKLTGMAFRVPTVDVSVVDLTVKLNKETTYDEIKKVVKAAAEGKLKGVLGYTEDAVVSSDFLGDSHSSIFDASAGIQLSPKFVKLVSWYDNEYGYSTRVVDLVEHVAKA 6CFFFEE7061BC36F 6CFFFEE7061BC36F tdh3 1 13 unspecified role unspecified role neutral component neutral component physical association physical association Table 1 1 (130) TDH3/P00359 Glyceraldehyde-3-phosphate dehydrogenase 3 77 35.7 Homotetramer 143 c1ct 1 8 unspecified role unspecified role neutral component neutral component physical association physical association Table 1 2 (200) ADE3/P07245 C-1-tetrahydrofolate synthase, cytoplasmic 54 102.2 Homodimer 204 adh1-1 1 3 unspecified role unspecified role neutral component neutral component physical association physical association Table 1 3 (280) ADH1/P00330 Alcohol dehydrogenase 1 485 36.8 Homotetramer 147 gdh1-1 1 12 unspecified role unspecified role neutral component neutral component physical association physical association Table 1 4 (320) DHE4/P07262 NADP-specific glutamate dehydrogenase 1 407 49.5 Homohexamer 297 met17-tdh3-erg10 1 13 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component 2 unspecified role unspecified role neutral component neutral component physical association physical association Table 1 5 (200) MET17/P06106 O-acetyl homoserine-O-acetyl serine sulphhydrylase 705 48.7 Homotetramer 195 TDH3/P00359 Glyceraldehyde 3-phosphate dehydrogenase 3 206 35.7 Homotetramer 142 ERG10/P41338 Acetyl-CoA acetyltransferase 82 41.7 Unknown (homotetramera)) 167 dhe4-ald6-tsa1 1 12 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component physical association physical association Table 1 6 (320) DHE4/P07262 NADP-specific glutamate dehydrogenase 1 801 49.5 Homohexamer 297 ALD6/P54115 Magnesium-activated aldehyde dehydrogenase, cytosolic 366 54.4 Unknown (homotetramerb)) 217 TSA1/P34760 Peroxiredoxin TSA1 245 21.6 Unknown (homomultimerc)) ,300 idh1-idh2-5 1 6 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component physical association physical association The complex was a hetero-octomer of the two proteins. Table 1 7 (420) IDH1/P28834 Isocitrate dehydrogenase subunit 1, mitochondrial 43 38 Hetero-octamer of IDH1 and IDH2 304 IDH2/P28241 Isocitrate dehydrogenase subunit 2,mitochondrial 69 37.8 hsp60-2 1 11 unspecified role unspecified role neutral component neutral component physical association physical association Table 1 HSP60/P19882 HSP60, mitochondrial 329 57.8 Heptamer 404 ape1-2 1 4 unspecified role unspecified role neutral component neutral component physical association physical association Table 1 8 (600) APE1/P14904 Vacuolar aminopeptidase 1 519 51.7 Homododecamer 620