IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Elongator controls the migration and differentiation of cortical neurons through acetylation of alpha-tubulin. Elongator controls the migration and differentiation of cortical neurons through acetylation of alpha-tubulin. Cell (0092-8674) 2009 imex curation Creppe C., Malinouskaya L., Volvert ML., Gillard M., Close P., Malaise O., Laguesse S., Cornez I., Rahmouni S., Ormenese S., Belachew S., Malgrange B., Chapelle JP., Siebenlist U., Moonen G., Chariot A., Nguyen L. Data pertaining to alpha-tubulin could not be curated as this could not be associated with a single gene product. alain.chariot@ulg.ac.be, lnguyen@ulg.ac.be 09-April-2009: Contacted by JYOTI. jyoti Only protein-protein interactions 4 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Creppe C., Malinouskaya L., Volvert ML., Gillard M., Close P., Malaise O., Laguesse S., Cornez I., Rahmouni S., Ormenese S., Belachew S., Malgrange B., Chapelle JP., Siebenlist U., Moonen G., Chariot A., Nguyen L. Data pertaining to alpha-tubulin could not be curated as this could not be associated with a single gene product. alain.chariot@ulg.ac.be, lnguyen@ulg.ac.be Accepted 2009-MAY-1 by JYOTI. 09-April-2009: Contacted by JYOTI. Cell (0092-8674) 2009 jyoti Only protein-protein interactions 4 imex curation Elongator controls the migration and differentiation of cortical neurons through acetylation of alpha-tubulin. Elongator controls the migration and differentiation of cortical neurons through acetylation of alpha-tubulin. Cell (0092-8674) 2009 imex curation Creppe C., Malinouskaya L., Volvert ML., Gillard M., Close P., Malaise O., Laguesse S., Cornez I., Rahmouni S., Ormenese S., Belachew S., Malgrange B., Chapelle JP., Siebenlist U., Moonen G., Chariot A., Nguyen L. Data pertaining to alpha-tubulin could not be curated as this could not be associated with a single gene product. alain.chariot@ulg.ac.be, lnguyen@ulg.ac.be 09-April-2009: Contacted by JYOTI. jyoti Only protein-protein interactions 4 human-ht_29 Homo sapiens HT-29 caucasian women with colon adenocarcinoma ht_29 HT-29 confirmed as human with IEF of AST, LDH established from the primary tumor of a 44-year-old Caucasian woman with colon adenocarcinoma in 1964; described to be heterotransplantable forming well-differentiated grade I tumors anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Creppe C., Malinouskaya L., Volvert ML., Gillard M., Close P., Malaise O., Laguesse S., Cornez I., Rahmouni S., Ormenese S., Belachew S., Malgrange B., Chapelle JP., Siebenlist U., Moonen G., Chariot A., Nguyen L. Data pertaining to alpha-tubulin could not be curated as this could not be associated with a single gene product. alain.chariot@ulg.ac.be, lnguyen@ulg.ac.be Accepted 2009-MAY-1 by JYOTI. 09-April-2009: Contacted by JYOTI. Cell (0092-8674) 2009 jyoti Only protein-protein interactions 4 imex curation Elongator controls the migration and differentiation of cortical neurons through acetylation of alpha-tubulin. Elongator controls the migration and differentiation of cortical neurons through acetylation of alpha-tubulin. Cell (0092-8674) 2009 imex curation Creppe C., Malinouskaya L., Volvert ML., Gillard M., Close P., Malaise O., Laguesse S., Cornez I., Rahmouni S., Ormenese S., Belachew S., Malgrange B., Chapelle JP., Siebenlist U., Moonen G., Chariot A., Nguyen L. Data pertaining to alpha-tubulin could not be curated as this could not be associated with a single gene product. alain.chariot@ulg.ac.be, lnguyen@ulg.ac.be 09-April-2009: Contacted by JYOTI. jyoti Only protein-protein interactions 4 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Creppe C., Malinouskaya L., Volvert ML., Gillard M., Close P., Malaise O., Laguesse S., Cornez I., Rahmouni S., Ormenese S., Belachew S., Malgrange B., Chapelle JP., Siebenlist U., Moonen G., Chariot A., Nguyen L. Data pertaining to alpha-tubulin could not be curated as this could not be associated with a single gene product. alain.chariot@ulg.ac.be, lnguyen@ulg.ac.be Accepted 2009-MAY-1 by JYOTI. 09-April-2009: Contacted by JYOTI. Cell (0092-8674) 2009 jyoti Only protein-protein interactions 4 imex curation Elongator controls the migration and differentiation of cortical neurons through acetylation of alpha-tubulin. Elongator controls the migration and differentiation of cortical neurons through acetylation of alpha-tubulin. Cell (0092-8674) 2009 imex curation Creppe C., Malinouskaya L., Volvert ML., Gillard M., Close P., Malaise O., Laguesse S., Cornez I., Rahmouni S., Ormenese S., Belachew S., Malgrange B., Chapelle JP., Siebenlist U., Moonen G., Chariot A., Nguyen L. Data pertaining to alpha-tubulin could not be curated as this could not be associated with a single gene product. alain.chariot@ulg.ac.be, lnguyen@ulg.ac.be 09-April-2009: Contacted by JYOTI. jyoti Only protein-protein interactions 4 human-sk_n_be(2) Human caucasian neuroblastoma cell line sk_n_be(2) Human neuroblastoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Creppe C., Malinouskaya L., Volvert ML., Gillard M., Close P., Malaise O., Laguesse S., Cornez I., Rahmouni S., Ormenese S., Belachew S., Malgrange B., Chapelle JP., Siebenlist U., Moonen G., Chariot A., Nguyen L. Data pertaining to alpha-tubulin could not be curated as this could not be associated with a single gene product. alain.chariot@ulg.ac.be, lnguyen@ulg.ac.be Accepted 2009-MAY-1 by JYOTI. 09-April-2009: Contacted by JYOTI. Cell (0092-8674) 2009 jyoti Only protein-protein interactions 4 imex curation elp3_human Elongator complex protein 3 ELP3 Protein lysine acetyltransferase ELP3 tRNA uridine(34) acetyltransferase protein protein human Homo sapiens Human MRQKRKGDLSPAELMMLTIGDVIKQLIEAHEQGKDIDLNKVKTKTAAKYGLSAQPRLVDIIAAVPPQYRKVLMPKLKAKPIRTASGIAVVAVMCKPHRCPHISFTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPFLQTRHRIEQLKQLGHSVDKVEFIVMGGTFMALPEEYRDYFIRNLHDALSGHTSNNIYEAVKYSERSLTKCIGITIETRPDYCMKRHLSDMLTYGCTRLEIGVQSVYEDVARDTNRGHTVKAVCESFHLAKDSGFKVVAHMMPDLPNVGLERDIEQFTEFFENPAFRPDGLKLYPTLVIRGTGLYELWKSGRYKSYSPSDLVELVARILALVPPWTRVYRVQRDIPMPLVSSGVEHGNLRELALARMKDLGIQCRDVRTREVGIQEIHHKVRPYQVELVRRDYVANGGWETFLSYEDPDQDILIGLLRLRKCSEETFRFELGGGVSIVRELHVYGSVVPVSSRDPTKFQHQGFGMLLMEEAERIAREEHGSGKIAVISGVGTRNYYRKIGYRLQGPYMVKMLK DIP protein Q9H9T3 original sequence version: 124 DIP protein Q9H9T3 original sequence version: 127 60898ADD3DBAD035 elp1_human Elongator complex protein 1 ELP1 IKAP IKBKAP p150 IkappaB kinase complex-associated protein protein protein human Homo sapiens Human MRNLKLFRTLEFRDIQGPGNPQCFSLRTEQGTVLIGSEHGLIEVDPVSREVKNEVSLVAEGFLPEDGSGRIVGVQDLLDQESVCVATASGDVILCSLSTQQLECVGSVASGISVMSWSPDQELVLLATGQQTLIMMTKDFEPILEQQIHQDDFGESKFITVGWGRKETQFHGSEGRQAAFQMQMHESALPWDDHRPQVTWRGDGQFFAVSVVCPETGARKVRVWNREFALQSTSEPVAGLGPALAWKPSGSLIASTQDKPNQQDIVFFEKNGLLHGHFTLPFLKDEVKVNDLLWNADSSVLAVWLEDLQREESSIPKTCVQLWTVGNYHWYLKQSLSFSTCGKSKIVSLMWDPVTPYRLHVLCQGWHYLAYDWHWTTDRSVGDNSSDLSNVAVIDGNRVLVTVFRQTVVPPPMCTYQLLFPHPVNQVTFLAHPQKSNDLAVLDASNQISVYKCGDCPSADPTVKLGAVGGSGFKVCLRTPHLEKRYKIQFENNEDQDVNPLKLGLLTWIEEDVFLAVSHSEFSPRSVIHHLTAASSEMDEEHGQLNVSSSAAVDGVIISLCCNSKTKSVVLQLADGQIFKYLWESPSLAIKPWKNSGGFPVRFPYPCTQTELAMIGEEECVLGLTDRCRFFINDIEVASNITSFAVYDEFLLLTTHSHTCQCFCLRDASFKTLQAGLSSNHVSHGEVLRKVERGSRIVTVVPQDTKLVLQMPRGNLEVVHHRALVLAQIRKWLDKLMFKEAFECMRKLRINLNLIYDHNPKVFLGNVETFIKQIDSVNHINLFFTELKEEDVTKTMYPAPVTSSVYLSRDPDGNKIDLVCDAMRAVMESINPHKYCLSILTSHVKKTTPELEIVLQKVHELQGNAPSDPDAVSAEEALKYLLHLVDVNELYDHSLGTYDFDLVLMVAEKSQKDPKEYLPFLNTLKKMETNYQRFTIDKYLKRYEKAIGHLSKCGPEYFPECLNLIKDKNLYNEALKLYSPSSQQYQDISIAYGEHLMQEHMYEPAGLMFARCGAHEKALSAFLTCGNWKQALCVAAQLNFTKDQLVGLGRTLAGKLVEQRKHIDAAMVLEECAQDYEEAVLLLLEGAAWEEALRLVYKYNRLDIIETNVKPSILEAQKNYMAFLDSQTATFSRHKKRLLVVRELKEQAQQAGLDDEVPHGQESDLFSETSSVVSGSEMSGKYSHSNSRISARSSKNRRKAERKKHSLKEGSPLEDLALLEALSEVVQNTENLKDEVYHILKVLFLFEFDEQGRELQKAFEDTLQLMERSLPEIWTLTYQQNSATPVLGPNSTANSIMASYQQQKTSVPVLDAELFIPPKINRRTQWKLSLLD DIP protein O95163 original sequence version: 140 DIP protein O95163 original sequence version: 116 5BAC580433CC8641 elp1-elp3-1 1 5 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association likely because of its inability to efficiently bind ELP3 in contrast to full-length ELP1 Fig. 4B elp1-elp3-2 4 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association ELP3 constitutively binds to ELP1 in multiple cell types. Supp. Fig. 10 elp1-elp3-3 2 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association ELP3 constitutively binds to ELP1 in multiple cell types. Supp. Fig. 10 elp1-elp3-4 3 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association ELP3 constitutively binds to ELP1 in multiple cell types. Supp. Fig. 10