MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it dMec: a novel Mi-2 chromatin remodelling complex involved in transcriptional repression. dMec: a novel Mi-2 chromatin remodelling complex involved in transcriptional repression. EMBO J. (0261-4189) 2009 imex curation Kunert N., Wagner E., Murawska M., Klinker H., Kremmer E., Brehm A. Only protein-protein interactions spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Kunert N., Wagner E., Murawska M., Klinker H., Kremmer E., Brehm A. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation dMec: a novel Mi-2 chromatin remodelling complex involved in transcriptional repression. dMec: a novel Mi-2 chromatin remodelling complex involved in transcriptional repression. EMBO J. (0261-4189) 2009 imex curation Kunert N., Wagner E., Murawska M., Klinker H., Kremmer E., Brehm A. Only protein-protein interactions drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kunert N., Wagner E., Murawska M., Klinker H., Kremmer E., Brehm A. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation dMec: a novel Mi-2 chromatin remodelling complex involved in transcriptional repression. dMec: a novel Mi-2 chromatin remodelling complex involved in transcriptional repression. EMBO J. (0261-4189) 2009 imex curation Kunert N., Wagner E., Murawska M., Klinker H., Kremmer E., Brehm A. Only protein-protein interactions drome-kc Drosophila melanogaster embryo KC cells kc Drosophila embryo KC cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kunert N., Wagner E., Murawska M., Klinker H., Kremmer E., Brehm A. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation dMec: a novel Mi-2 chromatin remodelling complex involved in transcriptional repression. dMec: a novel Mi-2 chromatin remodelling complex involved in transcriptional repression. EMBO J. (0261-4189) 2009 imex curation Kunert N., Wagner E., Murawska M., Klinker H., Kremmer E., Brehm A. Only protein-protein interactions drome-kc Drosophila melanogaster embryo KC cells kc Drosophila embryo KC cells biochemical biochemical fingerprinting peptide massfingerprinting fingerprinting Kunert N., Wagner E., Murawska M., Klinker H., Kremmer E., Brehm A. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation dMec: a novel Mi-2 chromatin remodelling complex involved in transcriptional repression. dMec: a novel Mi-2 chromatin remodelling complex involved in transcriptional repression. EMBO J. (0261-4189) 2009 imex curation Kunert N., Wagner E., Murawska M., Klinker H., Kremmer E., Brehm A. Only protein-protein interactions drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Kunert N., Wagner E., Murawska M., Klinker H., Kremmer E., Brehm A. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation dMec: a novel Mi-2 chromatin remodelling complex involved in transcriptional repression. dMec: a novel Mi-2 chromatin remodelling complex involved in transcriptional repression. EMBO J. (0261-4189) 2009 imex curation Kunert N., Wagner E., Murawska M., Klinker H., Kremmer E., Brehm A. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Kunert N., Wagner E., Murawska M., Klinker H., Kremmer E., Brehm A. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation dMec: a novel Mi-2 chromatin remodelling complex involved in transcriptional repression. dMec: a novel Mi-2 chromatin remodelling complex involved in transcriptional repression. EMBO J. (0261-4189) 2009 imex curation Kunert N., Wagner E., Murawska M., Klinker H., Kremmer E., Brehm A. Only protein-protein interactions drome-kc Drosophila melanogaster embryo KC cells kc Drosophila embryo KC cells cosedimentation cosedimentation western blot western blot Immuno blot Immuno blot Kunert N., Wagner E., Murawska M., Klinker H., Kremmer E., Brehm A. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation chdm_drome Chromodomain-helicase-DNA-binding protein Mi-2 homolog Mi-2 CG8103 ATP-dependent helicase Mi-2 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MASEEENDDNFQEEEEAQEDNAPAAELSNDSDAPLKPNNDEDDDYDPEDSRRKKKGKKRKTRKGEEKGRKKKKRKKNESEEDSDFVQHDEEVEYPSTSKRGRKRKEEKQAAKEKESASSGMPSVEDVCSAFSVCNVEIEYSEEELQSLTTYKAFMHHVRPILQKENPKIAAPKLVMLVAAKWREFCESNPHIQQEGGAAGSGGSAGQARSVTGDEPEEPRSSRSSRNEKPDDIYEEAVEEEEEEEEEEKKPRRKRSGRGKKGRRPSGKVPTLKIKLLGKRKRDSSDEEQDASGASERDSDLEFERMLQKSDDSADEKEAPVSSKADNSAPAAQDDGSGAPVVRKKAKTKIGNKFKKKNKLKKTKNFPEGEDGEHEHQDYCEVCQQGGEIILCDTCPRAYHLVCLEPELDEPPEGKWSCPHCEADGGAAEEEDDDEHQEFCRVCKDGGELLCCDSCPSAYHTFCLNPPLDTIPDGDWRCPRCSCPPLTGKAEKIITWRWAQRSNDDGPSTSKGSKNSNSRVREYFIKWHNMSYWHCEWVPEVQLDVHHPLMIRSFQRKYDMEEPPKFEESLDEADTRYKRIQRHKDKVGMKANDDAEVLEERFYKNGVKPEWLIVQRVINHRTARDGSTMYLVKWRELPYDKSTWEEEGDDIQGLRQAIDYYQDLRAVCTSETTQSRSKKSKKGRKSKLKVEDDEDRPVKHYTPPPEKPTTDLKKKYEDQPAFLEGTGMQLHPYQIEGINWLRYSWGQGIDTILADEMGLGKTIQTVTFLYSLYKEGHCRGPFLVAVPLSTLVNWEREFELWAPDFYCITYIGDKDSRAVIRENELSFEEGAIRGSKVSRLRTTQYKFNVLLTSYELISMDAACLGSIDWAVLVVDEAHRLKSNQSKFFRILNSYTIAYKLLLTGTPLQNNLEELFHLLNFLSRDKFNDLQAFQGEFADVSKEEQVKRLHEMLGPHMLRRLKTDVLKNMPSKSEFIVRVELSAMQKKFYKFILTKNYEALNSKSGGGSCSLINIMMDLKKCCNHPYLFPSAAEEATTAAGGLYEINSLTKAAGKLVLLSKMLKQLKAQNHRVLIFSQMTKMLDILEDFLEGEQYKYERIDGGITGTLRQEAIDRFNAPGAQQFVFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQANKVMIYRFVTRNSVEERVTQVAKRKMMLTHLVVRPGMGGKGANFTKQELDDILRFGTEDLFKEDDKEEAIHYDDKAVAELLDRTNRGIEEKESWANEYLSSFKVASYATKEEEEEEETEIIKQDAENSDPAYWVKLLRHHYEQHQEDVGRSLGKGKRVRKQVNYTDGGVVAADTTRDDSNWQDNGSEYNSEYSAGSDEDGGDDDFDDQNGAERKAKRRLERRDDRPLPPLLARVGGNIEVLGFNARQRKSFLNAIMRYGMPPQDAFNSQWLVRDLRGKSERNFKAYVSLFMRHLCEPGADNAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHINGYYSMPELILKPCEPVRSALKQDVAALEAPPTGGNVDKSATTSNSVTPATSAAPSPAPASEKGEDKDKDSEKEKDKTSAEKSEVKQEQEAEEDKKPGDVKQENPVEEAAGDTKPSDAEVKTEVAKTEPKEETKDPEVKEEPKTEEKEKEKVDDKKPIPPTTVIDDDDDDVMIVKEDGELEKPSASSPKDQKAVAAATSAATGATGKGAEDSLEVLKRKFMFNIADGGFTELHTLWLNEEKAAVPGREYEIWHRRHDYWLLAGIVTHGYGRWQDIQNDIRFAIINEPFKMDVGKGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNLAQDPSHPAMSLNARFAEVECLAESHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKSDVSRLPATLARIPPVAQRLQMSERSILSRLAATAGNASNAAQLMAQFPAGFQGTTLPAFTSGPAGNFANFRPQFSVPGQLSNNSGV ED8E256D1AD0AC2F ei3d1_drome Eukaryotic translation initiation factor 3 subunit D-1 eIF3d1 Eukaryotic translation initiation factor 3 subunit 7-1 Eukaryotic translation initiation factor 3 subunit p66 eIF-3p66 CG10161 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSETINTAAQFPSFEKPTVQFNEKGWGPCELPDTFKDVPYQPFSKNDRLGKICDWTNTSNNDKKYQNKYASSFGTGIQYSYYHEEDETTFHLVDTARVQKPPHQRGRFRNMRNSRSGRGRNARGGLNTHGMTTLSGKNVKARDPRHGRGMGKKFGHRGPPPKMRESSVAVRADWASIEEMDFPRLIKLSLPNIKEGVDIVTCGTLEYYDKTYDRINVKNEKPLQKIDRIVHTVTTTDDPVIRRLSKTVGNVFATDAILATIMCSTRSNYSWDIVIEKVGDKVFMDKRDHTEFDLLTVNESSVEPPTDDDSSCNSPRNLAIEATFINHNFSQQVLKTGDQEPKYKFEESNPFISEDEDIQVASVGYRYKKWELGSDIVLVARCEHDGVLQTPSGEPQFMTIKALNEWDSKLANGVEWRQKLDTQRGAVLANELRNNACKLAKWTVQAVLAGSDQLKLGYVSRINPRDHSRHVILGTQQFKPHEFATQINLSMDNAWGILRCIIDLVMKQKDGKYLIMKDPNKPIIRLYDIPDNTFDSDDSDDGEGDDEGFQQVYNYAHNKI B8A0E0C8977BA737 hdac1_drome Histone deacetylase HDAC1 HDAC1 Rpd3 CG7471 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQSHSKKRVCYYYDSDIGNYYYGQGHPMKPHRIRMTHNLLLNYGLYRKMEIYRPHKATADEMTKFHSDEYVRFLRSIRPDNMSEYNKQMQRFNVGEDCPVFDGLYEFCQLSAGGSVAAAVKLNKQASEICINWGGGLHHAKKSEASGFCYVNDIVLGILELLKYHQRVLYIDIDVHHGDGVEEAFYTTDRVMTVSFHKYGEYFPGTGDLRDIGAGKGKYYAVNIPLRDGMDDDAYESIFVPIISKVMETFQPAAVVLQCGADSLTGDRLGCFNLTVKGHGKCVEFVKKYNLPFLMVGGGGYTIRNVSRCWTYETSVALAVEIANELPYNDYFEYFGPDFKLHISPSNMTNQNTSEYLEKIKNRLFENLRMLPHAPGVQIQAIPEDAINDESDDEDKVDKDDRLPQSDKDKRIVPENEYSDSEDEGEGGRRDNRSYKGQRKRPRLDKDTNSNKASSETSSEIKDEKEKGDGADGEESTASNTNSNNNSNNKSDNDAGATANAGSGSGSGSGAGAKGAKENNI DIP protein Q94517 original sequence version: 67 B0F6503D42A1BCE9 q0e8j0_drome MEP-1 Dmel\CG1244 MEP1 Q0E8J0_DROME anon-EST:Liang-1.37 anon-WO0118547.584 clone 1.37 dMEP-1 Dmel_CG1244 CG1244 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTEVDVVLPEGVAEPTTKLASASSSPKQSKPSADDVEQQVTTVEDDEEQVVEEQQEEDVHLPEAPSNEKLELLSAGGKDISKEDVAQASPQAPVADEIMEVDGTEDVEENEVENDDEDSQITSSSSKEPKLDEEDEEATTNGDGDHEPEDEDDAQKIGSTAENSCEAEDPLGAVTVPDDELASGKKAHLDGEESVASEGVVEEEPPAKQENGFGASPNQKENGQASIAEARAAIAPSDGGVVNLDEDSDEEMEDITEHKEPAKPTEKAAKPASSSGGESSDDAVLIASDSDTESAAPPPPVKKLPPVVKAIPPPPVQDDEDDDDDDDCVVIEDDTPLSISPSGKRKSDLDDLQLQQPSHKRQRSSTPSGMGQLTIKDARSLMPLDGSPSSVSGPRDSIYPVTITNAVTGAATNLGVVPPKLVPMAGGMSASPVQLNPPTLSLTGLPSMTNNANLLPGLTDDMFVLEAPSFIVPYIYEKPPRENICEVVKAIETKYALSAEDLAEADKGDEFDMMTEQNKEDKPADQADESGKKKKKKRGDDESWSEQSDDDDDEDDDDDSESGVRTKVLIKEANDDLSALKVAIKPAAALATAGGVSVNNPGGNKPGQENYFESPLGKFFMDIGVGLVQEYVQSDLLRLQKRKMRKAQVQNSKEFEMAINALSGNLQASKTKNAPFKFRMKRCEFCNFKSESAMAMANHYETPHMNGVLYKCNFCTFEIRNATEIVYHMEAVHNIKARLIKPLPYHQCPNCGFEDNGKAKLARHQPVCAKKFRPELNLAPPNDWEAPAKIPRIKPRHGLVGTATAYQAMAAQAAAQKAALANIQQQQAAAQARNNLQAAALAAQNAAKMRQRAPQPPKQNIVRNPAPVRGGNAMNAGLSLPNSYQLAAGQLVQASKKPMAGQPSISITPLPRQSSVGAGAGASSSKAPQAAAGMKPGQSPSGNNKAQFVICEICDGYIKDLEQLRNHMQWMHKVKIHPKMIYNRPPLNCQKCQFRFFTDQGLERHLLGSHGLVTSSMQEAANKGKDAGRCPVCGRMYQWKLLNHVSRDHHMTLKPAHLSYKCTVCTATFGMYKQFETHVYTAHSTVARKAMDSKKNSAQSSGSGSGAGMSRSSLGAANDSLLKPLKINDEITIIPQPASKPRITNMESHVID 646324FE7D92954D mi_2-mep_1 4 dMi-2 8 fingerprinting peptide massfingerprinting fingerprinting predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level dMEP-1 11 fingerprinting peptide massfingerprinting fingerprinting predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association mint f1 st1 mep_1-mi_2 6 dMEP-1 11 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level dMi-2 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization f1c mint mep_1-mi_2-1 1 dMEP-1 11 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain flag tagged flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined dMi-2 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell physical association physical association f2c mint mep_1-mi_2-2 5 dMi-2 8 immunostaining immunostaining predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell dMEP-1 11 immunostaining immunostaining predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization F3 mint mep_1-mi_2-3 2 dMi-2 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level dMEP-1 11 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association mint The same interaction occurs in ovaries cells. f3d f5c mi_2-mep_1-1 2 dMi-2 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level dMEP-1 11 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f3d mint mi_2-eif_3p66 3 dMEP-1 11 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level dRpd3 10 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level dp66 9 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level dMi-2 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association homomint mint F5C rpd3-eif_3p66 3 dMi-2 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level dp66 9 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level dRpd3 10 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association homomint mint F5C eif_3p66-mi_2 3 dRpd3 10 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level dp66 9 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level dMi-2 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association homomint mint F5C eif_3p66-mep_1 7 dRpd3 10 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level dMi-2 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level dMEP-1 11 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level dp66 9 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization f5a homomint mint