IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Purification of low-abundance Arabidopsis plasma-membrane protein complexes and identification of candidate components. Purification of low-abundance Arabidopsis plasma-membrane protein complexes and identification of candidate components. Plant J. (0960-7412) 2008 imex curation Qi Y., Katagiri F. The authors used the following criteria to select potential complex members:(1) proteins identified in some replicates of the RPS2::HPB sample but never in any replicates of the control sample, with protein identification probability >50%; (2) identified at least three times with a protein identification probability of 90% or higher; (3) identified at least twice with coverage of two or more peptides (except for RIN4, which was identified once with three peptides and three times with one peptide). All proteins listed as interactors with the tagged protein complied with the confidence mapping criteria set out above. The authors used a tag comprising of the biotin carboxyl carrier protein domain (80 c-terminal amino acids of the Q42523 protein), PreScisson recognition site (GE Healthcare Life Sciences) and a HA epitope tag fused to the c-termini of proteins used in this study. The tagged proteins of interest were expressed in transgenic Arabidopsis plants which were crossed with the mcca mutant (which has a lesion within the Methylcrotonoyl-CoA carboxylase subunit alpha (Q42523) protein). Microsomes were isolated from Leaves and cross-linked with 1Mm DSP (Dithiobis[succinimidyl propionate]) and protein extracts were analysed as described in the text. katagiri@umn.edu 09-April-2009: Contacted by JYOTI. orchard Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress pull down pull down ms participant Identification by mass spectrometry ms participant Qi Y., Katagiri F. The authors used the following criteria to select potential complex members:(1) proteins identified in some replicates of the RPS2::HPB sample but never in any replicates of the control sample, with protein identification probability >50%; (2) identified at least three times with a protein identification probability of 90% or higher; (3) identified at least twice with coverage of two or more peptides (except for RIN4, which was identified once with three peptides and three times with one peptide). All proteins listed as interactors with the tagged protein complied with the confidence mapping criteria set out above. The authors used a tag comprising of the biotin carboxyl carrier protein domain (80 c-terminal amino acids of the Q42523 protein), PreScisson recognition site (GE Healthcare Life Sciences) and a HA epitope tag fused to the c-termini of proteins used in this study. The tagged proteins of interest were expressed in transgenic Arabidopsis plants which were crossed with the mcca mutant (which has a lesion within the Methylcrotonoyl-CoA carboxylase subunit alpha (Q42523) protein). Microsomes were isolated from Leaves and cross-linked with 1Mm DSP (Dithiobis[succinimidyl propionate]) and protein extracts were analysed as described in the text. katagiri@umn.edu Accepted 2009-APR-12 by ORCHARD. 09-April-2009: Contacted by JYOTI. Plant J. (0960-7412) 2008 orchard Only protein-protein interactions imex curation phot1_arath Phototropin-1 Non-phototropic hypocotyl protein 1 Root phototropism protein 1 PHOT1 JK224 NPH1 RPT1 T6D9_110 At3g45780 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEPTEKPSTKPSSRTLPRDTRGSLEVFNPSTQLTRPDNPVFRPEPPAWQNLSDPRGTSPQPRPQQEPAPSNPVRSDQEIAVTTSWMALKDPSPETISKKTITAEKPQKSAVAAEQRAAEWGLVLKTDTKTGKPQGVGVRNSGGTENDPNGKKTTSQRNSQNSCRSSGEMSDGDVPGGRSGIPRVSEDLKDALSTFQQTFVVSDATKPDYPIMYASAGFFNMTGYTSKEVVGRNCRFLQGSGTDADELAKIRETLAAGNNYCGRILNYKKDGTSFWNLLTIAPIKDESGKVLKFIGMQVEVSKHTEGAKEKALRPNGLPESLIRYDARQKDMATNSVTELVEAVKRPRALSESTNLHPFMTKSESDELPKKPARRMSENVVPSGRRNSGGGRRNSMQRINEIPEKKSRKSSLSFMGIKKKSESLDESIDDGFIEYGEEDDEISDRDERPESVDDKVRQKEMRKGIDLATTLERIEKNFVITDPRLPDNPIIFASDSFLELTEYSREEILGRNCRFLQGPETDLTTVKKIRNAIDNQTEVTVQLINYTKSGKKFWNIFHLQPMRDQKGEVQYFIGVQLDGSKHVEPVRNVIEETAVKEGEDLVKKTAVNIDEAVRELPDANMTPEDLWANHSKVVHCKPHRKDSPPWIAIQKVLESGEPIGLKHFKPVKPLGSGDTGSVHLVELVGTDQLFAMKAMDKAVMLNRNKVHRARAEREILDLLDHPFLPALYASFQTKTHICLITDYYPGGELFMLLDRQPRKVLKEDAVRFYAAQVVVALEYLHCQGIIYRDLKPENVLIQGNGDISLSDFDLSCLTSCKPQLLIPSIDEKKKKKQQKSQQTPIFMAEPMRASNSFVGTEEYIAPEIISGAGHTSAVDWWALGILMYEMLYGYTPFRGKTRQKTFTNVLQKDLKFPASIPASLQVKQLIFRLLQRDPKKRLGCFEGANEVKQHSFFKGINWALIRCTNPPELETPIFSGEAENGEKVVDPELEDLQTNVF DIP protein O48963 original sequence version: 137 C9B68812F1B3A04E mik2_arath MDIS1-interacting receptor like kinase 2 MIK2 Probable LRR receptor-like serine/threonine-protein kinase At4g08850 T32A17.160 At4g08850 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MNKTNPERKISLTSFKERMACKEKPRDLQVLLIISIVLSCSFAVSATVEEANALLKWKSTFTNQTSSSKLSSWVNPNTSSFCTSWYGVACSLGSIIRLNLTNTGIEGTFEDFPFSSLPNLTFVDLSMNRFSGTISPLWGRFSKLEYFDLSINQLVGEIPPELGDLSNLDTLHLVENKLNGSIPSEIGRLTKVTEIAIYDNLLTGPIPSSFGNLTKLVNLYLFINSLSGSIPSEIGNLPNLRELCLDRNNLTGKIPSSFGNLKNVTLLNMFENQLSGEIPPEIGNMTALDTLSLHTNKLTGPIPSTLGNIKTLAVLHLYLNQLNGSIPPELGEMESMIDLEISENKLTGPVPDSFGKLTALEWLFLRDNQLSGPIPPGIANSTELTVLQLDTNNFTGFLPDTICRGGKLENLTLDDNHFEGPVPKSLRDCKSLIRVRFKGNSFSGDISEAFGVYPTLNFIDLSNNNFHGQLSANWEQSQKLVAFILSNNSITGAIPPEIWNMTQLSQLDLSSNRITGELPESISNINRISKLQLNGNRLSGKIPSGIRLLTNLEYLDLSSNRFSSEIPPTLNNLPRLYYMNLSRNDLDQTIPEGLTKLSQLQMLDLSYNQLDGEISSQFRSLQNLERLDLSHNNLSGQIPPSFKDMLALTHVDVSHNNLQGPIPDNAAFRNAPPDAFEGNKDLCGSVNTTQGLKPCSITSSKKSHKDRNLIIYILVPIIGAIIILSVCAGIFICFRKRTKQIEEHTDSESGGETLSIFSFDGKVRYQEIIKATGEFDPKYLIGTGGHGKVYKAKLPNAIMAVKKLNETTDSSISNPSTKQEFLNEIRALTEIRHRNVVKLFGFCSHRRNTFLVYEYMERGSLRKVLENDDEAKKLDWGKRINVVKGVAHALSYMHHDRSPAIVHRDISSGNILLGEDYEAKISDFGTAKLLKPDSSNWSAVAGTYGYVAPELAYAMKVTEKCDVYSFGVLTLEVIKGEHPGDLVSTLSSSPPDATLSLKSISDHRLPEPTPEIKEEVLEILKVALLCLHSDPQARPTMLSISTAFS DIP protein Q8VZG8 original sequence version: 103 7C9E44DE99DAE8BF pip12_arath Aquaporin PIP1-2 PIP1-2 PIP1B TMPA Plasma membrane intrinsic protein 1b Transmembrane protein A F4I18.6 At2g45960 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEGKEEDVRVGANKFPERQPIGTSAQSDKDYKEPPPAPLFEPGELASWSFWRAGIAEFIATFLFLYITVLTVMGVKRSPNMCASVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAVYYIVMQCLGAICGAGVVKGFQPKQYQALGGGANTIAHGYTKGSGLGAEIIGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIFNKDNAWDDHWVFWVGPFIGAALAALYHVIVIRAIPFKSRS 80F12775B6809013 rin4_arath RPM1-interacting protein 4 RIN4 MJL12_1 At3g25070 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MARSNVPKFGNWEAEENVPYTAYFDKARKTRAPGSKIMNPNDPEYNSDSQSQAPPHPPSSRTKPEQVDTVRRSREHMRSREESELKQFGDAGGSSNEAANKRQGRASQNNSYDNKSPLHKNSYDGTGKSRPKPTNLRADESPEKVTVVPKFGDWDENNPSSADGYTHIFNKVREERSSGANVSGSSRTPTHQSSRNPNNTSSCCCFGFGGK DIP protein Q8GYN5 original sequence version: 96 DIP protein Q8GYN5 original sequence version: 68 AB391ECF840AEA09 patl1_arath Patellin-1 PATL1 T9N14.1 At1g72150 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAQEEVQKSADVAAAPVVKEKPITDKEVTIPTPVAEKEEVAAPVSDEKAVPEKEVTPEKEAPAAEAEKSVSVKEEETVVVAEKVVVLTAEEVQKKALEEFKELVREALNKREFTAPVTPVKEEKTEEKKTEEETKEEEKTEEKKEETTTEVKVEEEKPAVPAAEEEKSSEAAPVETKSEEKPEEKAEVTTEKASSAEEDGTKTVEAIEESIVSVSPPESAVAPVVVETVAVAEAEPVEPEEVSIWGVPLLQDERSDVILTKFLRARDFKVKEALTMLKNTVQWRKENKIDELVESGEEVSEFEKMVFAHGVDKEGHVVIYSSYGEFQNKELFSDKEKLNKFLSWRIQLQEKCVRAIDFSNPEAKSSFVFVSDFRNAPGLGKRALWQFIRRAVKQFEDNYPEFAAKELFINVPWWYIPYYKTFGSIITSPRTRSKMVLAGPSKSADTIFKYIAPEQVPVKYGGLSKDTPLTEETITEAIVKPAANYTIELPASEACTLSWELRVLGADVSYGAQFEPTTEGSYAVIVSKTRKIGSTDEPVITDSFKVGEPGKIVITIDNQTSKKKKVLYRFKTQ 1F0AC3BB0784FC5D rps2_arath Disease resistance protein RPS2 RPS2 Resistance to Pseudomonas syringae protein 2 F20B18.200 At4g26090 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDFISSLIVGCAQVLCESMNMAERRGHKTDLRQAITDLETAIGDLKAIRDDLTLRIQQDGLEGRSCSNRAREWLSAVQVTETKTALLLVRFRRREQRTRMRRRYLSCFGCADYKLCKKVSAILKSIGELRERSEAIKTDGGSIQVTCREIPIKSVVGNTTMMEQVLEFLSEEEERGIIGVYGPGGVGKTTLMQSINNELITKGHQYDVLIWVQMSREFGECTIQQAVGARLGLSWDEKETGENRALKIYRALRQKRFLLLLDDVWEEIDLEKTGVPRPDRENKCKVMFTTRSIALCNNMGAEYKLRVEFLEKKHAWELFCSKVWRKDLLESSSIRRLAEIIVSKCGGLPLALITLGGAMAHRETEEEWIHASEVLTRFPAEMKGMNYVFALLKFSYDNLESDLLRSCFLYCALFPEEHSIEIEQLVEYWVGEGFLTSSHGVNTIYKGYFLIGDLKAACLLETGDEKTQVKMHNVVRSFALWMASEQGTYKELILVEPSMGHTEAPKAENWRQALVISLLDNRIQTLPEKLICPKLTTLMLQQNSSLKKIPTGFFMHMPVLRVLDLSFTSITEIPLSIKYLVELYHLSMSGTKISVLPQELGNLRKLKHLDLQRTQFLQTIPRDAICWLSKLEVLNLYYSYAGWELQSFGEDEAEELGFADLEYLENLTTLGITVLSLETLKTLFEFGALHKHIQHLHVEECNELLYFNLPSLTNHGRNLRRLSIKSCHDLEYLVTPADFENDWLPSLEVLTLHSLHNLTRVWGNSVSQDCLRNIRCINISHCNKLKNVSWVQKLPKLEVIELFDCREIEELISEHESPSVEDPTLFPSLKTLRTRDLPELNSILPSRFSFQKVETLVITNCPRVKKLPFQERRTQMNLPTVYCEEKWWKALEKDQPNEELCYLPRFVPN DIP protein Q42484 original sequence version: 111 D279B6E30E49D640 esm1_arath GDSL esterase/lipase ESM1 ESM1 At3g14210 Protein EPITHIOSPECIFIER MODIFIER 1 Extracellular lipase ESM1 MAG2.6 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MADNLNLVSVLGVLLVLTIFHNPIIVYAGEGVPNVALFTFGDSYYDAGNKVFLSQRKDLPQTYWPYGKSRDYPNGKFSDGHIVPDFIADFISIPNGVLPPVLKPGVDISRGVSFAVADASILGAPVESMTLNQQVVKFKNMKSNWNDSYIEKSLFMIYIGTEDYLNFTKANPNADASAQQAFVTNVINRLKNDIKLLYSLGASKFVVQLLAPLGCLPIVRQEYKTGNECYELLNDLAKQHNGKIGPMLNEFAKISTSPYGFQFTVFDFYNAVLRRIATGRSLNYRFFVTNTSCCGVGTHNAYGCGKGNVHSKLCEYQRSYFFFDGRHNTEKAQEEMAHLLYGADPDVVQPMTVRELIVYPTGETMREYWEPNNLAIRRRPSRDFYLGLAAYY E49F3AF0EC586E72 ahm4_arath Cadmium/zinc-transporting ATPase 4 HMA3 F6G3.150 At4g30120 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAEGEESKKMNLQTSYFDVVGICCSSEVSIVGNVLRQVDGVKEFSVIVPSRTVIVVHDTFLISPLQIVKALNQARLEASVRPYGETSLKSQWPSPFAIVSGVLLVLSFFKYFYSPLEWLAIVAVVAGVFPILAKAVASVTRFRLDINALTLIAVIATLCMQDFTEAATIVFLFSVADWLESSAAHKASIVMSSLMSLAPRKAVIADTGLEVDVDEVGINTVVSVKAGESIPIDGVVVDGSCDVDEKTLTGESFPVSKQRESTVMAATINLNGYIKVKTTALARDCVVAKMTKLVEEAQKSQTKTQRFIDKCSRYYTPAVVVSAACFAVIPVLLKVQDLSHWFHLALVVLVSGCPCGLILSTPVATFCALTKAATSGFLIKTGDCLETLAKIKIVAFDKTGTITKAEFMVSDFRSLSPSINLHKLLYWVSSIECKSSHPMAAALIDYARSVSVEPKPDIVENFQNFPGEGVYGRIDGQDIYIGNKRIAQRAGCLTDNVPDIEATMKRGKTIGYIYMGAKLTGSFNLLDGCRYGVAQALKELKSLGIQTAMLTGDNQDAAMSTQEQLENALDIVHSELLPQDKARIIDDFKIQGPTMMVGDGLNDAPALAKADIGISMGISGSALATETGDIILMSNDIRKIPKGMRLAKRSHKKVIENVVLSVSIKGAIMVLGFVGYPLVWAAVLADAGTCLLVILNSMMLLRDEREAVSTCYRASTSSPVKLEEDEVEDLEVGLLQKSEETSKKSCCSGCCSGPKDNQQK 963396FD99AA35C7 hir3_arath Hypersensitive-induced response protein 3 P31 T4P13.3 HIR3 T22N4.8 At3g01290 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGNLFCCVLVKQSDVAVKERFGKFQKVLNPGLQFVPWVIGDYVAGTLTLRLQQLDVQCETKTKDNVFVTVVASIQYRVLADKASDAFYRLSNPTTQIKAYVFDVIRACVPKLNLDDVFEQKNEIAKSVEEELDKAMTAYGYEILQTLIIDIEPDQQVKRAMNEINAAARMRVAASEKAEAEKIIQIKRAEGEAESKYLSGLGIARQRQAIVDGLRDSVLGFAGNVPGTSAKDVLDMVMMTQYFDTMRDIGATSKSSAVFIPHGPGAVSDVAAQIRNGLLQANNAS 57A46F2A29CFB74C hir2_arath Hypersensitive-induced response protein 2 HIR2 T17F3.13 At1g69840 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGQALGCIQVDQSNVAIKETFGKFDEVLEPGCHCLPWCLGSQVAGHLSLRVQQLDVRCETKTKDNVFVTVVASIQYRALAESAQDAFYKLSNTRNQIQAYVFDVIRASVPKLDLDSTFEQKNDIAKTVETELEKAMSHYGYEIVQTLIVDIEPDVHVKRAMNEINAASRMREAASEKAEAEKILQIKRAEGEAESKYLSGMGIARQRQAIVDGLRNSVLAFSESVPGTSSKDVMDMVLVTQYFDTLKEIGASSKSNSVFIPHGPGAVRDIASQIRDGLLQGNSAAE 970254272F65D211 phot2_arath Phototropin-2 PHOT2 CAV1 KIN7 NPL1 Defective in chloroplast avoidance protein 1 Non-phototropic hypocotyl 1-like protein 1 K21L19.6 At5g58140 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MERPRAPPSPLNDAESLSERRSLEIFNPSSGKETHGSTSSSSKPPLDGNNKGSSSKWMEFQDSAKITERTAEWGLSAVKPDSGDDGISFKLSSEVERSKNMSRRSSEESTSSESGAFPRVSQELKTALSTLQQTFVVSDATQPHCPIVYASSGFFTMTGYSSKEIVGRNCRFLQGPDTDKNEVAKIRDCVKNGKSYCGRLLNYKKDGTPFWNLLTVTPIKDDQGNTIKFIGMQVEVSKYTEGVNDKALRPNGLSKSLIRYDARQKEKALDSITEVVQTIRHRKSQVQESVSNDTMVKPDSSTTPTPGRQTRQSDEASKSFRTPGRVSTPTGSKLKSSNNRHEDLLRMEPEELMLSTEVIGQRDSWDLSDRERDIRQGIDLATTLERIEKNFVISDPRLPDNPIIFASDSFLELTEYSREEILGRNCRFLQGPETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLFHLQPMRDQKGELQYFIGVQLDGSDHVEPLQNRLSERTEMQSSKLVKATATNVDEAVRELPDANTRPEDLWAAHSKPVYPLPHNKESTSWKAIKKIQASGETVGLHHFKPIKPLGSGDTGSVHLVELKGTGELYAMKAMEKTMMLNRNKAHRACIEREIISLLDHPFLPTLYASFQTSTHVCLITDFCPGGELFALLDRQPMKILTEDSARFYAAEVVIGLEYLHCLGIVYRDLKPENILLKKDGHIVLADFDLSFMTTCTPQLIIPAAPSKRRRSKSQPLPTFVAEPSTQSNSFVGTEEYIAPEIITGAGHTSAIDWWALGILLYEMLYGRTPFRGKNRQKTFANILHKDLTFPSSIPVSLVGRQLINTLLNRDPSSRLGSKGGANEIKQHAFFRGINWPLIRGMSPPPLDAPLSIIEKDPNAKDIKWEDDGVLVNSTDLDIDLF DIP protein P93025 original sequence version: 98 CA163E2596289737 rps2-rin4-rlk 1 7 unspecified role unspecified role bait bait c terminal hpb (HA�PreScission�Biotin) tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 2 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey physical association physical association Table 1