IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ AtMSH5 partners AtMSH4 in the class I meiotic crossover pathway in Arabidopsis thaliana, but is not required for synapsis. AtMSH5 partners AtMSH4 in the class I meiotic crossover pathway in Arabidopsis thaliana, but is not required for synapsis. Plant J. (0960-7412) 2008 imex curation Higgins JD., Vignard J., Mercier R., Pugh AG., Franklin FC., Jones GH. g.h.jones@bham.ac.uk orchard Only protein-protein interactions arath-pollen Arabidopsis thaliana pollen Pollen confocal microscopy confocal microscopy immunostaining immunostaining Higgins JD., Vignard J., Mercier R., Pugh AG., Franklin FC., Jones GH. g.h.jones@bham.ac.uk Accepted 2009-AUG-14 by ORCHARD. Plant J. (0960-7412) 2008 orchard Only protein-protein interactions imex curation msh4_arath DNA mismatch repair protein MSH4 FCAALL.423 dl4725w At4g17380 MSH4 MutS protein homolog 4 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEDDGGERSSFVAGLIENRAKEVGMAAFDLRSASLHLSQYIETSSSYQNTKTLLRFYDPSVIIVPPNKLAADGMVGVSELVDRCYSTVRKVVFARGCFDDTKGAVLIQNLAAEEPLALGLDTYYKQHYLSLAAAAATIKWIEAEKGVIVTNHSLTVTFNGSFDHMNIDATSVENLELIDPFHNALLGTSNKKRSLFQMFKTTKTAGGTRLLRANLLQPLKDIETINTRLDCLDELMSNEQLFFGLSQVLRKFPKETDRVLCHFCFKPKKVTEAVIGFENTRKSQNMISSIILLKTALDALPILAKVLKDAKCFLLANVYKSVCENDRYASIRKKIGEVIDDDVLHARVPFVARTQQCFALKAGIDGFLDIARRTFCDTSEAIHNLASKYREEFNLPNLKLPFNNRQGFFFRIPQKEVQGKLPNKFTQVVKHGKNIHCSSLELASLNVRNKSAAGECFIRTETCLEALMDAIREDISALTLLAEVLCLLDMIVNSFAHTISTKPVDRYSRPELTDSGPLAIDAGRHPILESIHNDFVSNSIFMSEATNMLVVMGPNMSGKSTYLQQVCLVVILAQIGCYVPARFATIRVVDRIFTRMGTMDNLESNSSTFMTEMRETAFIMQNVTNRSLIVMDELGRATSSSDGLAMAWSCCEYLLSLKAYTVFATHMDSLAELATIYPNVKVLHFYVDIRDNRLDFKFQLRDGTLHVPHYGLLLAEVAGLPSTVIDTARIITKRITDKENKRIELNCGKHHEIHRIYRVAQRLICLKYSRQTEDSIRQALQNLNESFTEERL E1DCE70401C57A65 rad51_arath DNA repair protein RAD51 homolog 1 RAD51 Rad51-like protein 1 At5g20850 F22D1.20 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTTMEQRRNQNAVQQQDDEETQHGPFPVEQLQAAGIASVDVKKLRDAGLCTVEGVAYTPRKDLLQIKGISDAKVDKIVEAASKLVPLGFTSASQLHAQRQEIIQITSGSRELDKVLEGGIETGSITELYGEFRSGKTQLCHTLCVTCQLPMDQGGGEGKAMYIDAEGTFRPQRLLQIADRFGLNGADVLENVAYARAYNTDHQSRLLLEAASMMIETRFALLIVDSATALYRTDFSGRGELSARQMHLAKFLRSLQKLADEFGVAVVITNQVVAQVDGSALFAGPQFKPIGGNIMAHATTTRLALRKGRAEERICKVISSPCLPEAEARFQISTEGVTDCKD DIP protein P94102 original sequence version: 92 08067F8D85A2E77D q56x17_arath protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MYKEVLRLRELFCFELCIRTSSVQQRTSCISDR 7F5CF031E17127B3 mlh1_arath DNA mismatch repair protein MLH1 MLH1 MutL protein homolog 1 Protein MUTL-HOMOLOGUE 1 F23J3.170 T8A17.9 At4g09140 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MIDDSSLTAEMEEEESPATTIVPREPPKIQRLEESVVNRIAAGEVIQRPVSAVKELVENSLDADSSSISVVVKDGGLKLIQVSDDGHGIRREDLPILCERHTTSKLTKFEDLFSLSSMGFRGEALASMTYVAHVTVTTITKGQIHGYRVSYRDGVMEHEPKACAAVKGTQIMVENLFYNMIARRKTLQNSADDYGKIVDLLSRMAIHYNNVSFSCRKHGAVKADVHSVVSPSRLDSIRSVYGVSVAKNLMKVEVSSCDSSGCTFDMEGFISNSNYVAKKTILVLFINDRLVECSALKRAIEIVYAATLPKASKPFVYMSINLPREHVDINIHPTKKEVSLLNQEIIIEMIQSEVEVKLRNANDTRTFQEQKVEYIQSTLTSQKSDSPVSQKPSGQKTQKVPVNKMVRTDSSDPAGRLHAFLQPKPQSLPDKVSSLSVVRSSVRQRRNPKETADLSSVQELIAGVDSCCHPGMLETVRNCTYVGMADDVFALVQYNTHLYLANVVNLSKELMYQQTLRRFAHFNAIQLSDPAPLSELILLALKEEDLDPGNDTKDDLKERIAEMNTELLKEKAEMLEEYFSVHIDSSANLSRLPVILDQYTPDMDRVPEFLLCLGNDVEWEDEKSCFQGVSAAIGNFYAMHPPLLPNPSGDGIQFYSKRGESSQEKSDLEGNVDMEDNLDQDLLSDAENAWAQREWSIQHVLFPSMRLFLKPPASMASNGTFVKVASLEKLYKIFERC 0497793964CA3700 msh4-msh5-2 1 4 unspecified role unspecified role neutral component neutral component 2 unspecified role unspecified role neutral component neutral component colocalization colocalization Figure 4 (e-h) Figure 5 Dual immunolocalization of AtMSH5 (red) and AtMSH4 (green) antibodies onto meiocytes of the Atmsh5-1 and Atmsh4 mutants at early and mid prophase I. rad51-msh5-1 1 3 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component colocalization colocalization Figure 4 (a-d) Dual immunolocalization ofAtMSH5 protein with AtRAD51, mlh1-msh5-2 1 4 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component colocalization colocalization Figure 4 (i-l) Dual-localization studies in the wild type revealed that at late zygotene 76 � 9.8% (n = 5) of the AtMLH1 foci co-localized with AtMSH5