IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A cyclin-dependent protein kinase, CDKC2, colocalizes with and modulates the distribution of spliceosomal components in Arabidopsis. A cyclin-dependent protein kinase, CDKC2, colocalizes with and modulates the distribution of spliceosomal components in Arabidopsis. Plant J. (0960-7412) 2008 imex curation Kitsios G., Alexiou KG., Bush M., Shaw P., Doonan JH. john.doonan@bbsrc.ac.uk orchard Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Kitsios G., Alexiou KG., Bush M., Shaw P., Doonan JH. john.doonan@bbsrc.ac.uk Accepted 2009-AUG-14 by ORCHARD. Plant J. (0960-7412) 2008 orchard Only protein-protein interactions imex curation cdkc2_arath Cyclin-dependent kinase C-2 CDKC-2 MXK3.19 At5g64960 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAAAAFGQLNLEEPPPIWGSRSVDCFEKLEQIGEGTYGQVYMAKEIKTGEIVALKKIRMDNEREGFPITAIREIKILKKLHHENVIHLKEIVTSPGRDRDDQGKPDNNKYKGGIYMVFEYMDHDLTGLADRPGLRFTVPQIKCYMKQLLTGLHYCHVNQVLHRDIKGSNLLIDNEGNLKLADFGLARSYSHDHTGNLTNRVITLWYRPPELLLGATKYGPAIDMWSVGCIFAELLNGKPILPGKTENEQLNKIYELCGSPDESNWPGVSKMPWYNQMKSSRPLKRRVREIYRHFDRHALELLEKMLVLDPSQRICAKDALDAEYFWTDPLPCDPKSLPTYESSHEFQTKKKRQQMRHNEEAAKKQKLQHPQQQHSRLPPQQHGVGQSHAAPLWPAGPNHPMNNNAPPPQIPAGGHYYGGKPRGGAPVPNRYPPSGNQTGGYNNQSRGGYSSGAYPPQGRGAPYGAGPRGPSGGYGVGPPNYSQGGGQYGGSGGSGRGQNPMGGARNQQYGWQP F348F40377CA3756 cyp63_arath Peptidyl-prolyl cis-trans isomerase CYP63 CYP63 CYPRS64 Cyclophilin-63 Cyclophilin-like protein CypRS64 MAA21.30 At3g63400 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTKKKNPNVFLDVSIGGDPVQRIVIELFADVVPKTAENFRALCTGEAGVGKSTGKPLHFKGSSFHRVIKGFMAQGGDFSNGNGTGGESIYGGKFSDENFRLDHDGAGVLSMANCGPNTNGSQFFILFKRQPHLDGKHVVFGKVVEGMAVIKKMELVGTSDGKPTSPVKIIDCGETSQIRAHDAAEREKGKSKKSNKNFSPGDVSDREAKETRKKESNEKRIKRKRRYSSSDSYSSSSDSDSDSESEAYSSSSYESSSSSDGKHRKRKSTTRHKGRRGERKSKGRSGKKKARPDRKPSTNSSSDTESSSSSDDEKVGHKAIKSVKVDNADQHANLDDSVKSRSRSPIRRRNQNSRSKSPSRSPVRVLGNGNRSPSRSPVRDLGNGSRSPREKPTEETVGKSFRSPSPSGVPKRIRKGRGFTERYSFARKYHTPSPERSPPRHWPDRRNFQDRNRDRYPSNRSYSERSPRGRFRSPPRRRSPPRYNRRRRSTSRSPDGYRRRLRDGSRSQSPRHRSRSQSPRKRQPISQDLKSRLGPQRSPIRGGRTSPAESLSPSHSPSPPGKRGLVSYAD 1137880ED360102B sr34_arath Serine/arginine-rich-splicing factor SR34 SR34 Pre-mRNA-splicing factor SF2 SF2 SR1 SR1 protein SRP34 F22D16.16 F22D16_30 At1g02840 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSSRSSRTVYVGNLPGDIREREVEDLFSKYGPVVQIDLKVPPRPPGYAFVEFDDARDAEDAIHGRDGYDFDGHRLRVELAHGGRRSSDDTRGSFNGGGRGGGRGRGDGGSRGPSRRSEFRVLVTGLPSSASWQDLKDHMRKGGDVCFSQVYRDARGTTGVVDYTCYEDMKYALKKLDDTEFRNAFSNGYVRVREYDSRKDSRSPSRGRSYSKSRSRSRGRSVSRSRSRSRSRSRSPKAKSSRRSPAKSTSRSPGPRSKSRSPSPRRSRSRSRSPLPSVQKEGSKSPSKPSPAKSPIHTRSPSR 6530F9CB628B8EFA cdck2-srp34 1 4 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 2 unspecified role unspecified role neutral component neutral component n-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Figure 4 Figure 5 CDKC2 co-localizes in vivo with the splicing factor SRp34 and cyclophilin CypRS64. The authors concluded that " CDKC2 localizes in the same regions of the nucleus as SRp34, but co-expression of the two proteins modifies the distribution of each protein". 5,6-dichloro-1-beta-D-ribobenzimidazole (DRB) cdck2-cyprs64 1 3 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 2 unspecified role unspecified role neutral component neutral component n-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Figure 4 CDKC2 co-localizes in vivo with the splicing factor SRp34 and cyclophilin CypRS64.